Embed Size (px)
DESCRIPTION
1. Expression of ectopic HCVc174 with fusion tags. 2. Affinity purification and comparative SDS-PAGE for identification of prominent differential protein bands. 4. C onfirmation of hnRNPH1, NF45, and C14orf166 as HCVc174-interacting candidates. 7. Cytoplasmic confinement of - PowerPoint PPT Presentation
Citation preview
1. Expression of ectopic HCVc174 with fusion tags
2. Affinity purification and comparative SDS-PAGE for identification of prominent differential protein bands
3. LC-MS/MS analysis of 10 selected protein bands for HCVc174-interacting candidates
4. Confirmation of hnRNPH1, NF45, and C14orf166 as HCVc174-interacting candidates
6. Subcellular distributions of the 3 candidates and prominent nuclear co-localization of HCVc174
7. Cytoplasmic confinement of the 3 candidates with HCVc during Con1-1b or JFH1-2a replication
5. Interaction of HCVc174 and NF45 is RNA-dependent
