1 April, 2005

Chapter C4.1 and C5.1

DNA Microarrays and Cancer

Overview• DNA microarrays allow the researcher to assay the

transcription of a number of genes simultaneously.

• This technique is especially informative regarding large-scale changes in gene expression.

• Global analysis of gene expression is of particular interest in the understanding of cancer - a disease of gene expression.

• Cancer cell type and progression status can be probed using mmicroarray methods.

Differentially labeled

cDNA sets:

green = control,

red = experimental

Hybridization

Microarray Data

Microarray and Northern Data

Clustering of Microarray Data

Global Shift in Gene Expression

Global Shift in Gene Expression

DLBCL Microarray Experiment

Clustering in the DLBCL Experiment

Discovery of DLBCL Subtypes

Clinical DLBCL Outcomes

Expression Changes in

Breast Cancer

Cluster Analysis

Microarray Detection of Genomic Rearrangements

Southern Confirmation