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( . 2550) 1. 1.1
65 3 sample mill ( 1 )
1.2
305 450 (1 : 15 w/v) 24
1.3 5 300
(1 : 10 w/v) 85 – 90 30
1.4 8.8
0.925 100 6
1.5 40 (1 : 50 w/v)
120 60
2.IR (KBr disc)
66
1. Merck
2. 70%, 95% Merck
3. Fisher Scientific
4. VWR International
5. Labscan Asia
6. Ajak Finechem
7. glucose – D Boot Manufacturing
8. acetic acid Merck
1. National
2. (rotary evaporator) Buchi
3. TLC (Silica Gel F 254 TLC
Aluminium Sheet)
Merck
4. tank 20 x 5 x 20 cm. -
5. Digital Balance Mettler – Toledo AB 204 – S
6. -
7. micro hematocrit tube Biomed
8. Haemacytometer -
9. Nippon Kayaku
67
Haemacytometer
Inoculum
Haemacytometer Haemacytometer
H
2 H scale
1 ( ) Haemacytometer
cover slip scale loop spore suspension
loop slide spore
suspension scale dropper spore
suspension Haemacytometer spore suspension
scale
spore suspension 1 – 2
68
2 A, B, C D E (Counting areas)
Haemacytometer
1. E
(small squares) 25
(smallest squares) 16
2. E 25 x 16 x 1/400
3. ( 1/10 ) 25 x 16 x 1/400 x 1/10
0.1
4. E Y 0.1
5. 1 1 1
1000
0.1 = Y
1000 (1 .) = Y x 1000 x 1/0.1
= Y x 1 x 104 /
.
69
6. A B C D
E 5
Z 1
= Z/5 x 1 x 10 4 /
7. Suspension
5-10
Wetting agent Tween 20
( )
Dropper Loop Suspension Scale Haemacytometer 1
Cover slip Spore suspension
8.
10 5 -10 6 /
70
(I)
(I) 0.0010 1 ml
spot TLC spray
inhibition area A mm calibration curve
(I) B µg
(I) C g 100 g
(I) 0.0010 g (100 x 0.0010) /C g
(I) B g (100 x 0.0010) /C g
1 g (I) BC/0.1 µg
A = inhibition area
B = (I) µg calibration curve
C = (I) g
(I)
90 chitosan 0.5
(I) 0.0010 g 1 ml spot
TLC TLC spray
inhibition area 14.5 mm calibration curve
(I) 1048.97 µg
(I) 0.1210 g 100 g
(I) 0.0010 g (100 x 0.0010) / 0.1210 g
(I) 1048.97 µg (100 x 0.0010) / 0.1210 g
1 g (I) = (1048.97 x 0.1210) / 0.1 µg
= 1269.25 µg
71
1 (I) 100PTLC 90, 100,110 120
.
( )
(I)
( )
0.50% 0.2478
0.2469
0.2462
0.2464
0.1210
0.1197
0.1190
0.1193
0.75% 0.2479
0.2482
0.2464
0.2470
0.1214
0.1209
0.1199
0.1195
1.00% 0.2485
0.2480
0.2469
0.2476
0.1217
0.1210
0.1208
0.1209
0.10%
+ 0.50%
0.2500
0.2493
0.2502
0.2489
0.1229
0.1220
0.1218
0.1220
0.10%
+ 0.75%
0.2491
0.2502
0.2497
0.2488
0.1228
0.1230
0.1225
0.1220
0.10%
+ 1.00%
0.2514
0.2501
0.2497
0.2500
0.1235
0.1230
0.1227
0.1229
72
1 ( ) (I) 100PTLC 90, 100, 110
120
.
( )
(I)
( )
0.2462
0.2454
0.2456
0.2450
0.1210
0.1187
0.1181
0.1160
73
2 (I) (I) 0.0010C. gloeosporioides
( ) inhibition area (mm)
(I)
0.50% 90
100
110
120
14.5
13.0
11.5
9.0
1048.97
933.58
818.20
625.89
0.75% 90
100
110
120
15.5
14.0
12.5
10.0
1125.89
1010.51
895.12
702.81
1.00% 90
100
110
120
16.0
15.0
13.5
12.0
1164.35
1087.43
972.05
856.66
0.10% + 0.50%
90
100
110
120
17.5
16.0
1535
14.5
1279.74
1164..35
1087.43
1048.97
0.10%
+ 0.75%
90
100
110
120
19.0
18.0
16.5
15.0
1395.12
1318.20
1202.82
1087.43
0.10%
+ 1.00%
90
100
110
120
20.5
19.0
17.5
16.0
1510.51
1395.12
1279.74
1164.35
74
2 ( ) (I) (I) 0.0010C. gloeosporioides
( ) inhibition area (mm)
(I)
90
100
110
120
14.0
11.5
9.5
7.0
1010.51
818.20
664.35
472.05
75
3 (I) 1
( )
(I) ( g)
1
0.50% 90
100
110
120
1269.25
1117.50
973.66
746.69
0.75% 90
100
110
120
1366.83
1221.71
1073.25
839.86
1.00% 90
100
110
120
1417.01
1315.79
1174.24
1035.70
0.10%
+ 0.50%
90
100
110
120
1572.80
1420.51
1324.49
1279.74
0.10%
+ 0.75%
90
100
110
120
1713.21
1621.39
1473.45
1326.66
0.10%
+ 1.00%
90
100
110
120
1865.47
1716.00
1570.24
1430.99
76
3 ( ) (I) 1
( )
(I) ( g)
1
90
100
110
120
1222.72
971.20
784.60
547.57
–
– – 6 2525 22/4 3 . . . 23120
6. 2543
2547
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