1

20

16

-06

CNIM Group

CORIOLIS µ, AN INNOVATIVE AIR SAMPLER

2

20

16

-06

CNIM Group

BERTIN INSTRUMENTS, MONITOR THE INVISIBLE

Bertin Instruments is a brand of CNIM Group

dedicated to innovative measurement and sampling solutions

for key worldwide markets

Lab Equipment

(sample preparation, air sampler)

Nuclear Instrumentation

(environmental radiation monitoring systems, radon professional monitoring, radiation portal monitors, health physics

equipment)

Defense, Security

and Safety (CBRN threat detection, gas

detection, optronics)

With the expertise of:

3

20

16

-06

CNIM Group

CONTAMINATION SOURCES & TRADITIONAL CONTROL

Sources of contamination • People • Raw materials • Water • Air

Monitoring by - Passive impaction - Active impaction - Filtration - Impinger

Hospital

Waste

Agriculture

Industry

Air in Building Pharma

4

20

16

-06

CNIM Group

10 YEARS OF WET CYCLONE TECHNOLOGY IMPLEMENTATION

MonaLisa Project 2006-2009

Validation of a new method for pollen and allergen detection

French Army Project Since 2006

Portable air sampler for airborne pathogens detection

Coriolis µ

Since 2009

Air Sampler for bio-contamination

quality control

5

20

16

-06

CNIM Group

CORIOLIS µ, COLLECT MICRO ORGANISMS INTO A LIQUID!

6

20

16

-06

CNIM Group

COMPLEMENTARY SOLUTION: CORIOLIS TECHNOLOGY

• Particles from 0.5 to 20 µm

• Bacteria, fungi, spores, viruses, pollens, allergens, endotoxins…

1- Pre-filled sterile cone with adapted collection liquid

3- Particles are concentrated into the collection liquid

2- Air & particles enter into the cone and form a vortex: aspirated particles are centrifuged with the collection liquid on the wall

Air

Collection liquid

Particles

7

20

16

-06

CNIM Group

HOW CORIOLIS GET OVER LIMITATIONS OF TRADITIONAL METHODS?

Limitation of traditional technologies

Only cultivable flora

Limited Air flow rate

Limited time of collection

Coriolis advantages

Allergens, virus, fungi…

High Air Flow rate (300 L/min)

Long time monitoring available (6h)

Result in days or weeks

Short response time in hours

• High air flow rate: 300 L/min

• Collection from 1 to 10 min,

• Up to 6 hours sampling with Long Time Monitoring

• Light: 3 kg

• Easy decontamination

• Battery operated

8

20

16

-06

CNIM Group

QUALIFICATION BY HPA - UK

Physical and biological efficiency of the Coriolis® technology are qualified according to ISO 14698-1 Annex 1- July 2008 requirements

• Physical efficiency: Bacillus atrophaeus NCTC 10073

• Biological efficiency of 78% for Staphylococcus epidermidis ATCC 14990

Size of interest

HPA Porton Down (UK)

d50 <0.5µm

100% for particles > 4 µm

9

20

16

-06

CNIM Group

COMPATIBLE WITH BIOLOGICAL ANALYSIS

10

20

16

-06

CNIM Group

DETAILED WORKFLOW FOR IDENTIFICATION BY CULTURE

Culture and results

24 hours

Sampling

10 min

Sample Preparation

10 min

11

20

16

-06

CNIM Group

DETAILED WORKFLOW FOR IDENTIFICATION BY QPCR

Sample prep

1 min

DNA extraction

30 min

qPCR reaction & analysis

60-90 min

Sampling

10 min

12

20

16

-06

CNIM Group

CORIOLIS RANGE

13

20

16

-06

CNIM Group

CORIOLIS µ PRODUCT

Biological Air Sampler for bio-contamination quality control • High air flow rate: 300 L/min

• Collection from 1 to 10 min,

• Up to 6 hours sampling with Long Time Monitoring

• Light: 3 kg

• Easy decontamination

• Battery operated

14

20

16

-06

CNIM Group

CORIOLIS

Ref. 05027-662-RD001

LTM Option for Coriolis µ air sampler includes :

1 x Platform for Coriolis µ

1 x LTM Tripod

1 x Air intake for LTM

1 x Collection liquid in bottle

1 x Tubing kit

1 x Shipment box

• Ref. 05027-030-RD003

• Coriolis µ air sampler includes :

• 1 x Coriolis body

• 1 x Air intake

• 1 x Cane

• 4 x Cones & Caps

• 1 x Battery & 1 Charger

• 1 x Power Supply

• 1 x User manual

• 1 x Shipment box

15

20

16

-06

CNIM Group

ACCESSORIES

Air Intake & LTM Air Intake

Autoclavable

Ref. 05027-600-003

Ref. 05027-662-PE103

Cane

Autoclavable

Ref. 05027-600-PE101

Air Intake with 25 mm

Connection

Autoclavble – adapted for pipe

connection

Ref. 05027-600-PE104

Exhaust Pipe connection

Adapted for clean rooms

Ref. 05027-030-ExPi

NiMH Battery and charger

Ref. 05027-622-RD001

Ref.05027-600-PL010

Coriolis µ platform and tripod

For outdoor collection

Ref. 05027-672-RD001

16

20

16

-06

CNIM Group

CONSUMABLES

Tubing kit

The tubing kit contains 1 meter

of tubing and a specific cap with

0,25µm filter for collection

liquid bottle.

Ref. 05237-1-204

Collection liquid in bottle

1x 250 mL bottle prefilled with

200 mL of collection liquid

Ref. 05237-1-203

Collection liquid doses sterile

(by 50)

50 units of sterile collection

liquid doses. 15mL each.

Ref. 05237-1-202

Sterile cones & caps -

Single use (by 50)

50 units of sterile cones

and caps. Each cone and

cap are packed in a single

sterile pouch.

Ref. 05237-1-101

Cones & caps - Single use (by

50)

50 units of cones and caps.

Ref. 05237-1-003

17

20

16

-06

CNIM Group

SERVICES

• Calibration – Once a Year

Specific calibration bench at our Support Center (Aix en Provence – FR)

Ref. FLOW CTRL

• Flow control – to control the flow by itself

Ref. 05027-340-RD001

After sales services – diagnostic and repair

Price on request

• Application Center

Accessible on the web site

18

20

16

-06

CNIM Group

APPLICATION & CASES STUDIES

19

20

16

-06

CNIM Group

POLLUTION & ENVIRONNENT

Outdoor surveillance / Bioburden

Indoor Air Quality: public health, prevention of diseases and allergies (e.g. in offices, child-care-centers, schools…)

Pollen dispersal / cross pollination / Crop diseases

• Example : Kiwi crop (bacteria disease PSA-V (Pseudomonas syringae pv actinidiae - virulent)

• Greenhouse cultivation • Field experiment

Epidemiology study (ex.: Oak oidium Inra 33 Cestas Dynamique temporelle de dépôts de spores)

20

20

16

-06

CNIM Group

CASE STUDY: COMPOSTING SITE

Investigation on the simultaneous presence of amoebae and Legionella in the compost piles and in the bioaerosols developed from pile fermentation

• 250 L/min, 4 min. sampling (1m3)

• Culture analysis

• The protozoa were cultured on non-nutritive agar plates covered with E.coli as a food source.

• Legionella spp. were cultivated on GVPC agar plates and by co-culture with axenic Acanthamoaba polyphaga for two weeks.

21

20

16

-06

CNIM Group

CASE STUDY: BARCELONA SUBWAY

Investigation of the air quality in the Barcelona Subway system during a cold period:

• 200L/min for 10 min

• Inside trains, platforms and lobbies

• Detection by QPCR & sequencing

• Low concentration of Influenza A&B

• High level of bacterial bioburden (104 bacteria/m3)

22

20

16

-06

CNIM Group

ANIMAL SCIENCE & VETERINARY

Coriolis can be used to monitor the air for potential contaminants that are a health risk

Areas with High animal densities / livestock houses / CAFO (Confined animal feeding operations) • Swine confinement building (barns), Turkey confinement house, Poultry barns,

Dairy barns, Duck-fattening unit, Cattle industries

Animal disease outbreak • Disease transmission among animals • ex.: foot and mouth (FAM) disease, avian influenza, Newcastle disease…)

23

20

16

-06

CNIM Group

CASE STUDY: POULTRY CONTAMINATION

Aflatoxin B₁ detection in Poultry units with qPCR. Aflatoxin B₁ has been recognized to produce cancer in human liver. • 300 L/min • 3 indoors samples from 2 different poultry

units • DNA extraction • Specific qPCR to validate detection

24

20

16

-06

CNIM Group

CASE STUDY: GLUTEN DETECTION

Number of gluten-free consumers has increased greatly in recent years. Goal: create a specific area in the production workshop dedicated to the production of gluten free products

• A1: 300L/min for 10 and 20 min when the machines were stopped and during production time

• A2: 300L/min for 10 and 20 min and at 200L/min for 5 min during production time

• Detection by ELISA Kit

Sampling parameters

[Gluten]

(mg of gluten/kg of

liquid collection)

[Gluten]

(mg of gluten/m3 of air)Results

Control (distilled water) <5 ppm - OK

A1-300L/min-20 min

Machines stopped<5 ppm

< 0,075 mg de gluten/6000L of air

< 12,5 µg de gluten/m3 of air

A1-300L/min-10 min

Machines stopped<5 ppm

< 0,075 mg de gluten/3000L of air

< 25 µg de gluten/m3 d'air

A1-300L/min-20 min

Production time<5 ppm

< 0,075 mg de gluten/6000L of air

< 12,5 µg de gluten/m3 of airLow gluten contamination

A1-300L/min-10 min

Production time8 ppm

0,12 mg de gluten/3000L of air

40 µg de gluten/m3 of air

Increase of the level of gluten

during the production

Low gluten contamination

Sampling parameters

[Gluten]

(mg of gluten/kg of

liquid collection)

[Gluten]

(mg of gluten/m3 of air)Results

A2-300L/min-20 min

Production time35 ppm

0,525 mg de gluten/6000L of air

87,5 µg de gluten/m3 of air

54 ppm0,81 mg de gluten/3000L of air

270 µg de gluten/m3 of air

44 ppm0,66 mg de gluten/3000L of air

220 µg de gluten/m3 of air

A2-200L/min-5 min

Production time18 ppm 270 µg de gluten/m3 d'air

A2-300L/min-10 min

Production time

Detection of gluten at a high

concentration -> the

production of gluten-free

products close to this area is

not possible

25

20

16

-06

CNIM Group

BIOMEDICAL & HEALTH

Coriolis can contribute to detect : • Virus and infectious agent such as Mers, H1N1, SRAS …

• Bacteria

• Fungi: Aspergillus

Early detection of pathogen can help limit the spreading in a hospital unit

Coriolis in routine can sustain high-quality of air and limit the risk of hospital-based infections

26

20

16

-06

CNIM Group

CASE STUDY: HOSPITAL CONTAMINATION

RSV (Respiratory Synticial Virus) detection in the surrounding patients • 300 L/min - 5min • Filtration and concentration of the sample • 30 air samples, at most 2 days after the diagnostic • Positive results by RT-PCR RSV real time for 6 samples for 3 children.

27

20

16

-06

CNIM Group

RESEARCH & DEVELOPMENT

Every topic related to bio-aerosol: • Lascaux Cave

• Archeologic Museum

• Amazon forest in Guyana

• Artic

• Coral reef barrier...

28

20

16

-06

CNIM Group

Epidemiological

investigation of MERS-CoV infections in bat or

camel flocks

Coriolis bio-aerosol

sampling surrounding

patients & medical staff

infected with MERS-

CoV

CASE STUDY: MERS SYNDROME-CORONAVIRUS DATA & PERSPECTIVES

Use of Coriolis

technology

Laboratory studies

with Coriolis μ

29

20

16

-06

CNIM Group

CONCLUSION

Innovative and comprehensive system

• High Air Flow Rate

• LTM Platform

• Easy to use

• Liquid Sampling Molecular Analysis

• Compatible with all Market and Application

30

20

16

-06

CNIM Group

www.bertin-instruments.com