02 Cell Structure & Function

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    2Cellular Level of Organization

    Detailed study of the cell began in the 1830s; some of the scientists contributing tothe understanding of cell structure and function were Robert Brown, MatthaisSchleiden, Theodor Schwann, and Rudolph Virchow.

    The cell theory states that all organisms are composed of cells, that cells are thestructural and functional unit of organisms, and that cells come only frompreexisting cells.

    Prokaryotic VS Eukaryotic Cells

    Prokaryotic cells lack a nucleus and are smaller and simpler than eukaryotic cells(which have a nucleus). Prokaryotic cells are placed in two taxonomic domains:Bacteria and Archaea. Organisms in these two domains are structurally similar butbiochemically different.

    Eukaryotic cells are members of the domain Eukarya, which includes the protists,fungi, plants, and animals. A membrane-bounded nucleushouses DNA; the nucleusmay have originated as an invagination of the plasma membrane. Eukaryotic cellsare much larger than prokaryotic cells, and therefore have less surface area per

    volume. Eukaryotic cells are compartmentalized; they contain small structures calledorganelles that perform specific functions. Some eukaryotic cells (e.g., plant cells)have a cell wallcontaining cellulose; plasmodesmata are channels in a cell wall thatallow cytoplasmic strands to extend between adjacent cells.

    The Structure of Eukaryotic Cells

    o Plasma membrane-

    porous, elastic structure that separates the contents of the cell from its

    surroundings;-

    acts as a boundary layer to contain the cytoplasm (fluid in cell)interlocking surfaces bind cells together

    -

    selectively permeable to select chemicals that pass in and out of cells

    Cell Structure & Function

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    o Endoplasmic reticulum-

    sheets of unit membrane with ribosomes on the outside; forms a tubulanetwork throughout the cell

    -

    transports chemicals between cells and within cells-

    provides a large surface area for the organization of chemical reactionsand synthesis

    -

    o Ribosomes-

    non-membraneous, spherical bodies composed of RNA (ribonucleic acidand protein enzymes

    -

    site of protein synthesis-

    o Golgi Apparatus-

    stacks of flattened sacs of unit membrane (cisternae) ; vesicles pinch offthe edges

    -

    modifies chemicals to make them functional; secretes chemicals in tinyvesicles ; stores chemicals ; may produce endoplasmic reticulum

    -

    o Lysosomes-

    membrane bound bag containing hydrolytic enzymes ; hydrolytic enzyme =(water split biological catalyst)

    -

    main function of these microbodies is digestion; lysosomes break downcellular waste products and debris from outside the cell into simplecompounds, which are transferred to the cytoplasm as new cell-buildingmaterials

    -

    o Mitochondria

    -

    composed of modified double unit membrane (protein, lipid) ; innemembrane infolded to form cristae-

    site of cellular respiration ie. the release of chemical energy from foodGlucose + Oxygen ------> Carbon Dioxide + Water + Energy (ATP)

    -

    o Peroxisomes-

    membrane-bounded vesicles that contain specific enzymes. Their actionresults in production of hydrogen peroxide.

    -

    Hydrogen peroxide (H2O2) is broken down to water and oxygen by

    catalase.Peroxisomes in the liver produce bile salts from cholesterol andalso break down fats. Peroxisomes also occur in germinating seeds where

    they convert oils into sugars used as nutrients by the growing plant.-

    o Vacuoles-

    a single layer of unit membrane enclosing fluid in a sac-

    produces turgor pressure against cell wall for support ; stores water andvarious chemicals ; may store insoluble wastes

    -

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    o Chloroplasts-

    membranous organelles (a type of plastid) that serve as the site ofphotosynthesis. The chloroplast is bound by a double membrane organizedinto flattened disc-like sacs called thylakoids formed from a thirdmembrane; a stack of thylakoids is a granum.

    -

    Chlorophyll and other pigments capture solar energy, and the enzymes

    which synthesize carbohydrates are located in the chloroplasts. Othetypes of plastids, which differ in color, form, and function fromchloroplasts, include chromoplasts and leucoplasts.

    -

    o Microfilaments-

    solid rods made of globular proteins called actin- these filaments are primarily structural in function and are an importan

    component of the cytoskeleton-

    o Microtubules-

    straight, hollow cylinders are found throughout the cytoplasm of aleukaryotic cells (prokaryotes don't have them) and carry out a variety offunctions, ranging from transport to structural support

    -

    o Cilia and Flagella-

    cilia and flagella are hair-like organelles that project from the surface ofsome cells

    -

    they cause cells to move through their medium or cause fluids or particlesto move over the cell surface

    -

    o Controsome and Centrioles

    -

    centrosome is the structure that occurs outside the nucleus of animal cellsis composed of two centrioles set at right angles to each other-

    centrosome plays a critical role in organizing the tubule system (mitoticspindle) that is involved in chromosome movement during cell division

    -

    centrosomes also organize the array of microtubules and microfilaments-

    o Nucleus-

    consists of the nuclear envelope, nucleolus, chromatin, and nucleoplasm-

    nuclear envelope is composed of two unit membranes with a fluid-filledspace ; nuclear pores present ; outer membrane may be continuous withendoplasmic reticulum ; selectively permeable to control movement in o

    out-

    chromatin is composed of long thin strands of DNA ; contains instructionsthat control cell metabolism and heredity

    -

    nucleolus is a composed of non-membraneous matrix of RNA (ribonucleicacid) and protein ; instructions in DNA are copied here ; works withribosomes in the synthesis of protein

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