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Center for Biomedical Research Support
THE UNIVERSITY OF TEXAS AT AUSTIN
Enabling Cutting-Edge Research that Changes the World
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Crystallography allows us to determine the three-dimensional structures of macromole-cules. Knowing molecular structure provides valuable insight into how molecules function in biological processes. Would knowing the molecular structure of a protein or nucleic acid help you in your research?
Why use X-ray Crystallography?
CrystallizationObtaining quality crystals that diffract well in the X-ray beam is key to solving a crystal structure. For rapidly preparing large numbers of crystallization experi-ments, the Facility has an Art Robbins Instruments Phoenix crystallization robot. A plate containing from 96 to 288 experiments can be prepared in about 15 minutes. Leading commercial crystalliza-tion screening kits are available for use. For the automated review of crystalliza-tion experiments, the Facility is equipped with the Art Robbins CrysCam. High power stereo microscopes are also available for manual review.
Macromolecular samples may be submit-ted to the facility for crystallization experi-
Macromolecular Crystallography Facility
CONTACTDR. ART [email protected]
ments. Training and assistance is available for interested users on the crystallization instruments.
We also offer programmed imaging of a crystallization plate with images taken on the CrysCam on multiple days following the plate set-up. Images may be viewed online.
biomedsupport.utexas.edu/
Fall ShortCourseIntroduction to PyMolwill be offered on WednesdayOctober 2nd, from 9 a.m. to 12 p.m. in FNT 1.104.
The course is free but registration is required.
To enroll, visit:ccbb.utexas.edu/shortcours-es.html
Our Facility features two complete X-ray crystallography systems for collecting crystal diffraction data, both mounted on the high flux Rigaku MicroMax 007HF X-ray generator. Our Facility also spon-sors and coordinates monthly shipments of crystals to the Advanced Light Source synchrotron for remote data collection. Training and assistance is available for interested users of the X-ray data collec-tion equipment. Data collection by expert staff is also available.
To support data processing and structure determination, our Facility has four dual core HP workstations installed with
Data Collection & Processingcurrent popular crystallographic software, including HKL2000, CCP4, Phenix, Coot, O, PyMOL, and more. The workstations are available for use by X-ray users. Data processing and structural analysis by expert staff are available.
Services
CENTER FOR BIOMEDICAL RESEARCH SUPPORT 2MACROMOLECULAR CRYSTALLOGRAPHY FACILITY
SERVICE DESCRIPTION
Phoenix Usage Use of the Art Robbins Instruments Phoenix crystallization robot to set up a plate of crystallization experiments.
X-ray usage Use of an RAXIS-IV++ detector on the Rigaku MicroMax-007 HF X-ray generator for the measurement of the diffraction of crystals.
SynchotronUsage
Shipment of crystals to a synchrotron beamline for data collection. Service includes the transfer and storage of diffraction data.
CrysCamUsage
Use of the Art Robbins CrysCam for automated visualization of crystallization experiments.
Programmed CrysCamImaging
LightCycler Usage
Imaging of a crystallization plate on days 1, 2, 4, and 8 following experiment set-up.
Experiment by Staff
Training
Workshop
Use of the Roche LightCycler 480 for Differential Scanning Fluorimetry or other application.
Experiment performed by staff expert. May include crystallization, X-ray data collection, data processing, and analysis.
Training of a user on an instrument.
Instruction and tutorials covering a particular method used in crystallography.
