WaterEnvironment&ReuseFoundation

ResearchOverviewNYWEA/NEWEASpringMeeting

Mystic,CTJune5–8,2016

* WhoisWE&RF?* RegulatoryChanges* EmergingConcerns* NewTechnologies

Outline

Ø  Conveneexpertsandsupportresearchtoacceleratetheadop2onofnewwatertechnologies

Ø  35-40reportspublishedannuallythatarehousedinanonline,searchabledatabase

Ø  Serveasaresearchhubforthewaterqualitycommunity(u2li2es,policymakers,consultants,universi2es,andindustry)

WhatdoesWERFdo?

DeliverBalancedResearch

FosterInnovation

DisseminateResults

CreateCollaboration

Ø  Managepeer-reviewedresearchtodeliver2mely,ac2onableresults

Recreational Water Quality Criteria

*  2012RecreationalWaterQualityCriteria*  2004BacteriaRuleforCoastalandGreatLakesRecreation*  1986RecreationalWaterQualityCriteria

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Allassociatedwithbacterialindicatorsoffecalcontamination(Fecalcoliform,E.coli,Enterococci)

BEACHAct(2010)

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Requiresstates,territories,andtribesthathavecoastalrecreationwaterstoadoptneworrevisedwaterqualitystandardsforpathogensandpathogenindicatorsforwhichEPAhaspublishedcriteriaunderCWAsection304(a).

Requiresanupdateevery5years–lastupdatewasin2012.

MovetoViralRecreationalWaterQualityCriteria

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NewCriteria

Epidemiology

Treatability

AnalyticalMethods

ResearchNeeds

• Istherearelationshipbetweenmalespecificand/orsomaticcoliphagewithentericvirusesinrecreationalwaters?

RelationshipBetweenViralLoadsandIndicator

• Istherearelationshipbetweenmalespecificand/orsomaticcoliphagewithhumanhealthinrecreationalwaters?Ifso,whatisthatrelationship?

• Istherearelationshipbetweenentericvirusesandhumanhealthinrecreationalwaters?Ifso,whatisthatrelationship?

• Doanyofthesepaperslinkcoliphageorvirusesoriginatingfromwastewaterthatisdischargedbycentralizedfacilitiestohumanhealth?

RelationshipBetweenIndicatorandHumanHealth

Outcomes

ReviewofColiphagesasPossibleIndicatorsofFecalContaminationforAmbientWaterQuality(WERF9C15)

EvaluatingFateofColiphagesinWRRFsandPotentialCoststoReduceColiphagesinWRRFEffluents(U3R15)ProjectObjectives

v  Gapanalysisonfate&treatabilityof

coliphagesinWRRFs.v  Fateofcoliphagesandfecalindicator

bacteriaatnineWRRFsusingdifferenttreatmentconfigurations&disinfectants.

v  Disinfectionofcoliphages&fecalindicatorbacteriaatbenchscale.

v  EconomicimpactsonWRRFsofcoliphage-basedRecreationalWaterQualityCriteria.

EmergingConcerns

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AntibioticResistanceCarbapenem-resistantenterobacteriaceae(CRE)

EmergingPathogensEbolaLegionellaZika

PrincipalInvestigator: AmyPruden,JohnNovakVirginiaTechAdditionalPartners:DCWater,HamptonRoadsSanitationDistrict,Dynamita,LuminUltraTech

WE&RFARResearch

WERFProjectWERF1C15Occurrence,Proliferation,andPersistenceofAntibioticsandAntibioticResistanceduring

WastewaterTreatment

*2-yearprojectstartedMay2015Finalreport-April2017

PrincipalInvestigators Dr. Daniel Gerrity, Assistant Professor Civil and Environmental Engineering and Construction University of Nevada, Las Vegas (UNLV) Co-Principal Investigators: Dr. Channah Rock, Associate Professor and Water Quality Specialist Soil, Water & Environmental Science University of Arizona (U of A) Dr. Jean McLain, Associate Director Water Resources Research Center University of Arizona (U of A) Dr. Eric Dickenson, R&D Project Manager Applied Research & Development Center Southern Nevada Water Authority (SNWA) Dr. Jacimaria Batista, Professor Department of Civil and Environmental Engineering and Construction University of Nevada, Las Vegas (UNLV)

FrameworkforAntibioticResistanceResearch

GoalUltimately,optimizebiologicaltreatmentsystemstosimultaneouslyreduceconcentrationsofTOrCs,includingantibiotics,andalleviatethespreadofARwithinthemicrobialcommunity.

ResearchQuestions

* Whatisthecurrentstateofthesciencerelatedtoantibioticresistanceinwastewater?* Howdoessolidsretentiontime(SRT)affectantibioticresistanceduringbiologicalwastewatertreatment?* Howdoantibioticconcentrationsaffectantibioticresistanceduringbiologicalwastewatertreatment?* Howdoesantibioticresistancechangethroughoutawastewatertreatmenttrainwithmultipleformsoffiltrationanddisinfection?

Longer SRTs achieve lower TOrC concentrations, but longer SRTs also provide prolonged bacterial exposure to influent levels of antibiotics and antibiotic resistance in an environment that is conducive to horizontal gene transfer… 1.  Do longer SRTs lead to greater

relative abundance of AR bacteria and genes?

2.  Do longer SRTs lead to greater extent of antibiotic resistance (i.e., resistance to antibiotics at higher concentrations)?

TOrC AR

SRT Pr

eval

ence

Critical Value

RemainingQuestions

WhatProjectSuccessWillLookLike!!!GENERALAnexpandedARknowledgebase/toolboxinwastewatertomitigatepotentialecological&publichealtheffectsupondischargeoruseoftreatedeffluent

Astudytocharacterizetherelevanceofwastewater-derivedARonecological&publichealth

StableoperationoftheSBRsduringeachphaseoftestingasindicatedbycontinuousautomation&anticipatedchangesinwaterqualityparameters

EXPERIMENTALHighqualitydatasetthatdemonstrateswhetherarelationshipexistsbetweenSRT,antibioticresistance,&effluentTOrCHighqualitydatathatdemonstratesifarelationshipexistsbetweeninfluentantibioticconcentrations,antibioticresistance,&effluentTOrCHighqualitydatasetthatillustrateshowprevalenceofARchangesatafull-scalewastewatertreatmenttrain

*  WEF/WE&RFWebcastonAntibioticResistance&theGlobalStatusofResearchJune22,2016(GWRC,SingaporePUB,andWE&RF)

*  WEF/WE&RFWebcastonAddressingConcernsonCarbapenem-resistanceEnterobacteriaceaeinWastewater-

July14,2016at1pmET(UNC,CDC,EPA)*  Seekingopportunitiestoclosetheresearch/knowledgegaps

on*  ABRhotspotsinwater(hospitalwastewatereffluent,overflow,etc.)inrelationtootherhotspots(soil,sludge,reuse,irrigation)*  Casestudies(results,methods,matrix)

UpcomingActivities

EmergingPathogensEbolaLegionellaZika

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EbolaResearchinWastewater

InpartnershipwiththeNationalScienceFoundation(NSF),theCaliforniaAssociationofSanitationAgencies(CASA),andothersFourProjects:1.  PotentialforExposuretoEbolaVirusSurrogatesAerosolizedfrom

WastewaterSystems(WERF2C15)2.  ProtectingWastewaterTreatmentPlantOperatorsfromEmerging

Pathogens(WERF3153.  RiskofEbolaDischargefromHospitaltoSewerWorkers(WERF4C15)4.  SurvivalofEbolaSurrogatesinWastewaterSystems(WERF5C15)

RisksfromEbolaDischargefromHospitals

toSewerWorkers

PrincipalInvestigators: CharlesHaasandTaylorRycroft,DrexelUniversity;KyleBibbyandLeonardCasson, UniversityofPittsburgh

EbolaResearchNextSteps

v  Summer2016–Allprojectswrappingup

v  June2016-WorkshoponscienceandsolutionstoprotectworkersfrominfectiousdiseasebeyondEbola

v  August2016-WE&RF/WEFWebinaronPersonalProtectiveEquipmentPPE

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DisinfectionTechnologiesUVPAA

www.werf.org/liH

ProgramComponents

1.  TechnologyEvalua2onProgram

2.  PeopleandPolicy3.  Communica2on4.  InformalForumfor

R&DManagers

•  DesignandImplementationofPeraceticAcidforMunicipalWaterandWastewaterRelatedProcesses(LIFT14T16)

•  ~2yearduration.Currentlyundernegotiation

•  DesignandValidationProtocolforUVDisinfectionSystemsUsedinMunicipalWastewaterTreatmentandReuseApplications(ENER16C15)

•  2yearproject;PurdueUniversity

LIFTProjects

GetInvolved

•  ParticipateintheLIFTDisinfectionWorkGroupCall(June15,20163PMET)

•  Joinupcomingwebcasts•  WEF/WE&RFWebcastonAntibioticResistance&theGlobalStatusof

Research(June22,2016)•  WEF/WE&RFWebcastonAddressingConcernsonCarbapenem-resistance

EnterobacteriaceaeinWastewater(July14,2016at1pmET)•  WE&RF/WEFWebinaronPersonalProtectiveEquipmentPPE(August

2016)•  SignupforWERFUpdates

•  Newsletters•  TargetedEmails

•  BringnewresearchtoWERF•  UnsolicitedProgram•  TCRProgram

ThankYou!

LolaOlabode,MPHProgramDirector(571)384-2109

Lolabode@werf.org

AllisonDeinesDirectorofSpecialProjects

(571)384-2116adeines@werf.org

www.werf.org

Questions