No: BT/PR3130/INF/22/139/2011, dated 15/10/2013

DEPARTMENT OF BIOTECHNOLOGY,MINISTRY OF SCIENCE & TECHNOLOGY

GOVERNMENT OF INDIA, NEW DELHI

PROGRESS REPORT(Infrastructure Programme)

A. PROJECT SUMMARY

1. Project Title: JNU-DBT National Training Facility for State-of-the-Art Equipment Used in Biotechnology Research

2. Date of Start of the project (as per sanction order): BT/PR3130/INF/22/139/2011, dated 15/10/2013

3. Report Duration (Date wise e.g. 02.05.2016 to 01.05.2017): 01.05.2016 to 5.12.20174. Total cost of the project (including revision): Rs. 6,18,72,000 (Six Crore Eighteen Lakh

and Seventy Two Thousand)5. Coordinator Detail: Prof. Rakesh K. Tyagi

Director,Advanced Instrumentation Research Facility (AIRF)Jawaharlal Nehru UniversityNew Delhi-110067

6. Sanctioned objectives and objectives achieved (Tabular format):Sanctioned objectives Objectives achieved1) To provide classroom theoretical and practical training by conducting seminars/workshops/ advanced courses for researchers/faculty members.

1) Classroom theoretical and practical training were provided by conducting 17 seminars/workshops/ advanced courses for researchers/faculty members.

2) To contribute to the intellectual input in research to aid innovation/ process.

2) We have facilitated visit by students/researchers/faculties and foreign delegates under the educational visit programme to enhance the user base and application of sophisticated techniques and designing future experiments.

3) To expand, upgrade and maintain a state-of-the-art instrumentation facility catering to the scientific community of JNU and other universities/research organizations, to ensure quality research at affordable cost.

3) The support from the DBT has resulted in CMC/AMC of 26 sophisticated instruments, reduction in downtime of the instruments and upgradation of some of the instrument facilities at AIRF. Consequently two new facilites under the Imaging programme have also been upgraded and added to the existing set of instruments.

7. Reason for non-achievement of objectives (Bullet points only): NA

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8. Infrastructure established (as per sanction order): NA9. Manpower trained (as per sanction order, plz include the break up with figures only

e.g. number of M.Sc. Students/JRF/SRF/RA and Assistant Professor, etc.): 6 nos. ( 3 Technical Officers and 3 Research Associate - M.Sc.1, M. Tech.2 & Ph D. 3)

10. Number of SC/ST/OBC category people trained (Plz provide figures separately for each category): NA

11. Research outcome: Publications (figures only; Kindly include only those publications having DBT’s acknowledgement as per the sanction order and comes out of this project ONLY in a year): More than18 papers published with the support of DBT. Patents (filed and granted separately; kindly also include the patent numbers/reference number): NilTechnology developed, transferred or commercialized (kindly include the name of the company to which the technology has been commercialized): NilStatus of commercialized technology (whether in market or not; if yes the name of the country): Nil

12. Non-recurring grant remaining unspent along with justification: Nil

B. PROGRESS REPORT IN DETAIL

1. Status of the Civil Works/Building Construction (kindly provide justification, if required): NA

2. Facilities Established (including the up-gradation of existing laboratories and facilities):

Details of upgradation of Imaging facilities are as follows:

Atomic Force Microscope (AFM) with Confocal Raman spectroscopy (Installed in October 2016).

Field Emission Scanning Electron Microscope (FESEM) and FESEM based e-beam lithography (Installed in October 2016).

ION Milling system for TEM/SEM samples Preparation Lab for Physical Sciences/ Material Science (Installed in October, 2016).

Upgradation of TIRF in Live Cell Imaging Laboratory: STORM is ready for installation.

3. Equipments purchased (as per sanction order): NA4. Research Details (not more than four pages including some good figures; kindly note

that this information should not be mere reproduction of research articles): AIRF provided classroom theoretical and practical training by conducting

seminars/workshops/ advanced courses for researchers/faculty members. Details of the seminars/workshops/ advanced courses as follows:

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S. No.

Title of seminars/workshops/ advanced courses Dates

1. Workshop on Electron Paramagnetic Resonance Spectroscopy

11th March, 2016

2. Workshop on F. A. C. S (I) 12th – 13th April, 2016

3. Workshop on Schrodinger Software 04th – 5th July, 2016

4. Workshop on Confocal Microscopy and its Application 11th – 12th August, 2016

5. Workshop on F. A. C. S. (II) 30th – 31st August, 2016

6. Workshop on Physical Property Measurement System and its Applications

28th September, 2016

7. Workshop on TEM for Material Sciences 24th – 25th November, 2016

8. Workshop on Metabolomics (Screening / Targeted Metabolomics)

18th -20th December, 2016

9. Workshop on Biological Sample Preparation for TEM/ SEM 24 - 25th January, 2017

10. Workshop on Combined Confocal Raman- AFM System 2nd - 3rd February, 2017

11. Workshop on Gas Chromatography - Mass Spectrometry: Principles and Applications

28th April, 2017

12. Workshop on Field Emission Scanning Electron Microscopy Based Lithography

4th - 5th May, 2017

13. Workshop on Combined Confocal Raman - AFM System 30th June, 2017

14. Workshop on Energy Dispersive X-Ray Fluorescence Spectrometer (EDXRF) and its applications

12th July, 2017

15. Lecture-cum-Demonstration on Emerging Applications on Potentiostat-Galvanostat

8th August, 2017

16. Workshop on Time Resolved Fluorescence Spectroscopy & Applications

6th October, 2017

17. Workshop on Circular Dichroism Spectroscopy and its Research Applications

24th November, 2017

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FIGURE 1.

A view of Workshop on Metabolomics

Seminars/workshops/ advanced courses have created awareness and training among the research community for the effective utilization of research facility leading to the substantial increase in the number of efficient users. The Workshops and Training Programmes are conducted to provide efficient and trained professionals to maintain a pace in the development of research in the country. The increased manpower has helped in maintaining the smooth functioning of facility. During the past year the number of publications have also improved the quality of the research publications has also seen marked change.

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Detail of participants attended Workshop/Training in AIRF

No: BT/PR3130/INF/22/139/2011, dated 15/10/2013

AIRF contributed the intellectual input in research to aid innovation/ process. We have facilitated visit by students/researchers/faculties and foreign delegates under the educational visit programme to enhance the user base and application of sophisticated techniques and designing future experiments. The details of students/researchers/faculties/foreign delegates and spectrum of user base from different Universities/Institutes are as follows:

Participants of 1st Interdisciplinary Course in Contemporary Studies organized by Academic Staff College visited on 26th February 2016

Faculty members from Mississippi State University, USA visited on 11th March 2016 Students of Amity University Haryana, Manesar visited on April 05th, 2016 Participants of HRDC visited on August12th, 2016. Students of Botany Dept, Sri Aurbindo College, (DU), visited on October 18th, 2016. Participants of 2nd Refresher Course (organized by UGC-HRDC, JNU), visited on

October 20th, 2016. Students of Dept. of Biochemistry, Shivaji College, (DU), visited on October 26th,

2016. Students of K R Mangalam University, Gurgaon, visited on November 4th, 2016. Foreign delegates from the EACEA (an agency of the European Commission), visited

on November 15th, 2016. Participants of course on Structural Biology under GIAN is being held at SLS, JNU

visited on November 18th, 2016. Live demonstration of instruments housed at AIRF for students of Amity University

Haryana, Manesar, on April 05th, 2016 by Dr. Gajender Saini, AIRF Live demonstration of instruments housed at AIRF for HRDC participants to AIRF on

August12th 2016. Live demonstration with class room theoretical of instruments housed at AIRF for

students of Botany department, Sri Aurbindo College, Delhi University, on October 18th, 2016 by Dr. Gajender Saini, AIRF.

Visit of participants of 2nd Refresher Course in Life Sciences & Biotechnology organized by UGC HRDC, JNU, on October 20th, 2016.

Visit of students of Department of Biochemistry, Shivaji College, University of Delhi on October 26th, 2016.

Visit of students of School of Basic & Applied Sciences, K R Mangalam, Gurgaon, on November 4th, 2016.

Live demonstration of instruments housed at AIRF for delegates from the EACEA (an agency of the European Commission), BRAVE co-ordinator - Dr. Andreas Voloudaikis and other European and Indian Scientists on November 15th, 2016.

Professor Carw Potti, University of HILAN, ITAY visited on November 15th, 2016 Visit of participants of course on Structural Biology under GIAN is being held at SLS,

JNU on November 18th, 2016. Visit of participants of workshop on Urban Air Pollution in India and UK Cities:

Characterization and Prediction of Chemically Reactive Air Pollutants in association with British Council on November 30th, 2016.

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Professor Debu Chakrabarti, from North –Western University, Chiago, IL, USA on December 9th, 2016

Live demonstration of instruments housed at AIRF for Human Resource Development Centre (HRDC) participants, 1st Refresher Course in Chemistry, January 11th, 2017

Handique Girl’s College, Guwahati, Post Graduate 16 students visited AIRF on January 16th, 2017

Live demonstration of instruments housed at AIRF for the students of Gragi College, University of Delhi visited AIRF on January 18th, 2017

Live demonstration of instruments housed at AIRF for 23 students, Department of Environmental Sciences, University of Jammu, February 10th ,2017

Visit by the participants of the workshop on Urban Air Pollution in

India and UK Cities

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No: BT/PR3130/INF/22/139/2011, dated 15/10/2013

60%19%

1%

18%

1%

Relative Number of AIRF Users (%)JNU UsersDelhi Academic UsersDelhi Industry usersOutside Delhi Academic UsersOutside Delhi Industry users

FIGURE 2. Relative Number of AIRF users

AIRF has about 4000 users coming from different parts of India. Our effort has lead to higher number of users and more application of sophisticated techniques leading to improvement into data quality.

AIRF expanded, upgraded and maintainted the state-of-the-art instrumentation facility catering to the scientific community of JNU and other universities/research organizations, to ensure quality research at affordable cost.The support from the DBT has resulted in reduction in downtime of the instruments and upgradation of some of the instrument facilities at AIRF. Consequently four new facilites have been added to the existing set of instruments. Details of upgradation of facilities are as follows:

Atomic force microscope (AFM) with Confocal Raman spectroscopy (Installed in October 2016).

Field Emission Scanning Electron Microscope (FESEM) and FESEM based e-beam lithography (Installed in October 2016).

ION Milling system for TEM/SEM samples Preparation Lab for Physical Sciences/ Material Science (Installed in October, 2016).

Upgradation of TIRF in Live Cell Imaging Laboratory: STORM is ready for installation.

Due to upgradation of the facilities and reduction in downtime of the instruments there is marked improvement in revenue generation. The detail of revenue generated during 2015-16 is given below :

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No: BT/PR3130/INF/22/139/2011, dated 15/10/2013

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No: BT/PR3130/INF/22/139/2011, dated 15/10/2013

JNU45%

External53%

Industrial1%

Revenue share 2016-2017

FIGURE 4: Revenue Share 2016-2017

Conclusions of the Progress made so far:With DBT support AIRF has achieved its objectives with following accomplishments during current year of the project:

Helped in the advancement of research by providing state-of-art equipments to JNU scientific community, outside researchers from other institutes/Universities and R & D labs in Industries.

Facilitated inter-disciplinary academic and research programs within JNU and outside. Conducted 17 workshops for Graduate students, Post Graduate Students, Research

Scholars, Post Docs and Faculties on the state-of-art equipments at AIRF. Trained more than 300 graduate students, Post Graduate students, Research Scholars,

Post Docs and Faculty on the state-of-art equipments. Facilitated in the publication of more than 19 research papers in reputed scientific

journals. More than 500 Graduate students, Post Graduate students, Research Scholars, Post Docs,

Faculties and foreign deleagates visited AIRF under the educational visit programme. Four new sophisticated instruments have been installed during 2016-2017:

Atomic force microscope (AFM) with Confocal Raman spectroscopy (Installed in October 2016)

Field Emission Scanning Electron Microscope (FESEM) and FESEM based

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No: BT/PR3130/INF/22/139/2011, dated 15/10/2013

e-beam lithography (Installed in October 2016) TEM/SEM samples Preparation Lab for Physical Sciences/ Material Science (ION

Milling installed in October, 2016) Upgradation of TIRF in Live Cell Imaging Laboratory: STORM is ready for

installation.

AIRF has successfully organized the training program on Electron Microscopy-I in 2015 and is planning to extend the professional training program/certificate course for Microscopy-2, Mass spectroscopy and X-Ray Diffraction (XRD) in view of negligible number of trained personals in these areas for an effective running and managing of research facility in different institutes, universities and industries.

To strengthen the research in interdisciplinary sciences, up gradation and expansion of afew instruments have been strategized and initial steps have been taken as following -

Super Resolution for Live Cell imaging (under process) Transmission Electron Microscope(TEM) for Biological Samples (under process) Inductively Coupled Plasma Mass Spectroscopy (ICPMS) (under process) Superconducting Quantum User interference device (SQUID) (under process) X-Ray Photoelectron Spectroscopy (XPS) (under process)

During the project current period almost 90% of the instruments have been covered under CMC & AMC.

During the project current period AIRF generated ˜ 86 lakhs as revenue. AIRF have also taken two initiatives to fulfill the obligation of acknowledging DBT

in publications and other forums- AIRF as research facility which attarcts Researchers form across the world

including Nepal, Srilanka, Bhutan and Europe has been highlighted in Asian Age Newspaper. In the newpaper article it was clearly mentioned that AIRF is jointly supported and maintained by funds from JNU and the Department of Biotechnology, Govt. of India. (Annexure A - copy of the news article attached).

In the AIRF requisition forms, it has specifically mentioned that DBT should be acknowledged in the publications arising out of the usage of the AIRF Instrument (Annexure B - copy of the AIRF requisition forms attached).

In order to disseminate the information about AIRF, fliers detailing AIRF activities have been published and distributed amongst users. In the flier it has been explicitly acknowledged that AIRF is supported and maintained by DBT (Annexure C - copy of the flier enclosed).

5. Research Outcome details - List of Publications Nirbhay Kumar Kushwaha, Mansi Bhardwaj, Supriya Chakraborty (2017). The

replication initiator protein of a geminivirus interacts with host monoubiquitination machinery and stimulates transcription of the viral genome. PLOS Pathogens. doi.org/10.1371/journal.ppat.1006587.

Monisha Gopalani, Alisha Dhiman, Amit Rahi, Divya Kandari, Rakesh Bhatnagar (2016). Identification, Functional Characterization and Regulon Prediction of a Novel Two Component System Comprising BAS0540- BAS0541 of Bacillus anthracis. PLOS ONE. 10.1371/journal.pone.0158895.

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Y Bagbi, A Sharma, HB Bohidar, PR Solanki (2016). Immunosensor based on nanocomposite of nanostructured zirconium oxide and gelatin-A International journal of biological macromolecules

PK Gupta, ZH Khan, PR Solanki (2016). One-Step Electrodeposited Porous ZnO Thin Film Based Immunosensor for Detection of Vibrio cholerae Toxin Journal of The Electrochemical Society.

Arun Singh Patel, HarekrushnaSahoo, T. Mohanty (2016) Investigating the Energy Transfer from Dye Molecules to DNA Stabilized Au Nanoparticles. J. Fluoresc. 2016, 26(5):1849-55.

Jyoti Shakya, Arun Singh Patel, Fouran Singh, and Tanuja Mohanty (2016). Composition dependent Fermi level shifting of Au decorated MoS2 nanosheets. Applied Physics Letters. 2016, 108, 013103.

Tyagiet al. (2016). Mechanistic evaluation of the size dependent antimicrobial activity of water soluble QDs. Analytical Methods, 2016, 8, 1060-1068.

Nisha Pawar, Kamla Rawat and   H. B. Bohidar (2016). Self-assembly of synthetic liposome-like curcumin nanoparticles. RSC Adv., 6, 73677-73682.

Kishan Das,   KamlaRawat and   H. B. Bohidar (2016). Morphology Dependent Interaction of Anisotropic Nanoparticles with Globular Plasma Proteins. RSC Adv., 6, 104117-104125

Nidhi Joshi,   Abhimanyu Sharma,   KamlaRawat, K. Asokan,   P. R. Solanki,   G. B. V. S. Lakshmi,  D. Kanjilal and   H. B. Bohidar (2016). Comparative Evaluation of Enzyme-Free Nanoclay-Ionic liquid based Electrodes for detection of bioanalytes. RSC Adv., 6, 66120 – 66129.

Kishan Das,  KamlaRawat, Rajan Patel and  H. B. Bohidar (2016). Size Dependent CdSe Quantum Dot-Lysozyme Interaction and Effect on Enzymatic Activity. RSC Adv., 6, 46744-4675.

Jyoti Shakya, Harekrushna Sahoo, and TanujaMohanty (2016). A study on the interaction between molybdenum disulfide and rhodamine B by spectroscopic methods. Journal of Material Science. DOI 10.1007/s10853-016-0640-y.

P. Neha, P Srivastava (2016). Synthesis and characterization of Bi deficient Bi3Ni superconductor, AIP

Sudesh, P. Kumar,P. Neha,T. Das, A. K. Rastogi, and S. Patnaik1, (2016). Evidence of trivial Berry phase and absence of chiral anomaly in semimetal NBP,arXiv:1608.06587.

Rawal, MK, Banerjee A, Shah AH, Khan MF, Sen S, Saxena AK, Monk BC, Cannon RD, Bhatnagar R, Mondal AK, Prasad R (2016). Newly identified motifs in Candida albicans Cdr1 protein nucleotide binding domains are pleiotropic drug resistance subfamily-specific and functionally asymmetric. Nature Sci Rep, 6, 27132.

Yogendra Kumar, Sharvan Kumar, Sudhir Kumar Keshri, Jyoti Shukla, Shiv Shankar Singh, Tejender S. Thakur, Mitchell Denti, Antonio Facchetti, and Pritam Mukhopadhyay (2016). Synthesis of OctabromoperyleneDianhydride and Diimides: Evidence of Halogen Bonding and Semiconducting Properties.Org. Lett., 18, 472–475.

Moirangthem Kiran Singh, Him Shweta and Sobhan Sen (2016). Dispersed Dynamics of Solvation in G-Quadruplex DNA: Comparison of Dynamic Stokes Shifts of Probes Bound to Parallel and Antiparallel Quadruplex Structures. Methods Appl. Fluoresc, 4, 034009.

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Srikanta Dana, Sudhir Kumar Keshri, Jyoti Shukla, KunwarSomeshVikramdeo, Neelima Mondal, Pritam Mukhopadhyay, and Suman Kumar Dhar (2016). Design, Synthesis and Evaluation of BifunctionalAcridinine−Naphthalenediimide Redox-Active Conjugates as Antimalarials. ACS Omega, 1, 318–333.

Vikrant Jayant and Dinabandhu Das (2016). 1,4-Dioxane-Specific Organic Hosts and Their Polymorphism.Cryst. Growth Des., 16 (8), 4183–4189.

- List of Patents (filed or granted): Nil- List of Technology Developed/Transferred/Commercialized : Nil

6. Recommendations of the last year Task Force /Site Visit /Expert Committee and their implementation status: NA

7. Challenges/Issues related to the project (needs Department of Biotechnology Consideration; Bullet points ONLY): NA

8. Enclosures, if any: Annexure-A, B & C.

(PI/coordinator of the Programme) (Head of the Institution)Director/AIRF

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Annexure-A

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Annexure-A

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Annexure-B

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Annexure-C

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