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Transcriptomic characterization of Quahog Parasite Unknown (QPX) and the effects of temperature on gene expression. Annie Page-Karjian, Maya Groner, Jamie Sziklay, Ana Elisa Garcia, Ashton Griffin, Gregor-Fausto Siegmund, Sonia Singhal, Steven Roberts. Thraustochytrids. - PowerPoint PPT Presentation
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Transcriptomic characterization of Quahog Parasite Unknown
(QPX) and the effects of temperature on gene expression
Annie Page-Karjian, Maya Groner, Jamie Sziklay, Ana Elisa Garcia, Ashton Griffin, Gregor-Fausto Siegmund,
Sonia Singhal, Steven Roberts
- Unicellular protists - Poorly described taxonomic group- Mostly saprophytes, but includes some highly virulent pathogens
THRAUSTOCHYTRIDS
QPX PATHOLOGY
- Massive inflammatory response
- Swelling and nodules mantle and labial palps
- Slower growth
Perrigault M et al. 2010
Environment Host
Pathogen
Disease
Brothers et al. 2000
OBJECTIVES OF OUR STUDY
1. Sequence and annotate the QPX transcriptome
2. Evaluate the effects of temperature on QPX gene expression
3. Identify biological function of enriched genes
10 ˚C 21 ˚C
Illumina Sequencing
De novo assembly
RNA seq
Enrichment analysis
2x
613 genes are differentially expressed and associated with
enriched processes!
45,082,610 reads
30,035,397 reads
11,280 contigs
OBJECTIVES OF OUR STUDY
1. Sequence and annotate the QPX transcriptome
2. Evaluate the effects of temperature on QPX gene expression
3. Identify biological function of enriched genes
RESULTS: TRANSCRIPTOME CHARACTERIZATION
RESULTS: TRANSCRIPTOME CHARACTERIZATION
OBJECTIVES OF OUR STUDY
1. Sequence and annotate the QPX transcriptome
2. Evaluate the effects of temperature on QPX gene expression
3. Identify biological function of enriched genes
RESULTS: DIFFERENTIAL GENE EXPRESSION
OBJECTIVES OF OUR STUDY
1. Sequence and annotate the QPX transcriptome
2. Evaluate the effects of temperature on QPX gene expression
3. Identify biological function of enriched genes
RESULTS: ENRICHMENT ANALYSISFo
ld in
crea
se
Different biological processes
Developmental processes (4)
Signal transduction (6)
RNA metabolism (110)
Protein metabolism (67)
DNA metabolism (9)
Stress response (13)
Cell organization and biogenesis
(10)
Cell cycle and proliferation (14)
Transport (49)
Cell adhesion (1)
RESULTS: GENETIC CONNECTIONS BETWEEN PROCESSES
Death (2)
Brothers et al. 2000
RESULTS: THE PHYSIOLOGICAL LINK
Protein FoldingRibosomal BiogenesisLipid Biosynthesis
BONUS!
BONUS! GENOME CHARACTERIZATION
QPX
Phytophthora
CONCLUSIONS
Environment Host
Pathogen
Disease
ACKNOWLEDGEMENTS
Questions?