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Š ï U V · 1 m dp = 320 nm CV = 12.9 % Biomass H O O O H Lactic acid n Polylactide (PLA) 0 ö ? Ê Â í º / < à Ç i É æ L Ñ S P y È%&>Ô4 )F%&>Õ>Ì %&>Ì>Ô

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Page 1: Š ï U V · 1 m dp = 320 nm CV = 12.9 % Biomass H O O O H Lactic acid n Polylactide (PLA) 0 ö ? Ê Â í º / < à Ç i É æ L Ñ S P y È%&>Ô4 )F%&>Õ>Ì %&>Ì>Ô

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Page 2: Š ï U V · 1 m dp = 320 nm CV = 12.9 % Biomass H O O O H Lactic acid n Polylactide (PLA) 0 ö ? Ê Â í º / < à Ç i É æ L Ñ S P y È%&>Ô4 )F%&>Õ>Ì %&>Ì>Ô

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Page 3: Š ï U V · 1 m dp = 320 nm CV = 12.9 % Biomass H O O O H Lactic acid n Polylactide (PLA) 0 ö ? Ê Â í º / < à Ç i É æ L Ñ S P y È%&>Ô4 )F%&>Õ>Ì %&>Ì>Ô

OO

OO

yx

O OO

Hn

O

N

OCH

CH3CH3

NH m

ONH

Ol

O OO

Hn

(PDMA-g-PDLLA)

1 �m

dp = 320 nm CV = 12.9 %

Biomass H O O

O

H

nLactic acid Polylactide (PLA)

O/W

30

25

20

15

10

5

0Ave

rage

par

ticle

dia

met

er (�

m)

100806040200Continuous phase flow (�L/min)

30

25

20

15

10

5

0

CV

(%)

CV

NH

O

ONal

O

NH

O

O

ONaO

O

Isopropylamine modified poly(succinimide) (IPA-PSI)

IPA-PSI

Heating Cooling

1 �m

Drying up

Aqueous IPA-PSI solution

Dehydrated IPA-PSI

Protein

Hydrated IPA-PSI

Heating Particle formation

0.12

0.10

0.08

0.06 0.04

0.02

0.00 0.20 0.15 0.10 0.05 0.00

Initial concentration of proteins (wt%)

Phycocyanin Hemoglobin Cytochrome c

Enc

apsu

late

d pr

otei

n (g

/ g-

poly

mer

)

100

80

60

40

20

0 Enc

losi

ng e

ffici

ency

(%)

0.20 0.15 0.10 0.05 0.00

Initial concentration of proteins (wt%)

Phycocyanin Cytochrome c < Hemoglobin

PAspNa

PSI

PSI

PSI

PSI

PSI

50 �m

R-NH2

pH

PSIPAsp

L-Aspartic acid Poly(aspartic acid) (PAsp)

Polysuccinimide (PSI)

(�m)

0.01 1 10 0.1 100

20 �m

20 �m

20 �m

PFOB

PFOB

PLA

PFOB

; perfluorooctyl bromide (PFOB)

PLA

30 �m 20 �m

: PAspNa

400 nm

TEM SEM

http://www.process.ecm.okayama-u.ac.jp/

PLA

1 �m

Enclosing efficiency

PSI

Page 4: Š ï U V · 1 m dp = 320 nm CV = 12.9 % Biomass H O O O H Lactic acid n Polylactide (PLA) 0 ö ? Ê Â í º / < à Ç i É æ L Ñ S P y È%&>Ô4 )F%&>Õ>Ì %&>Ì>Ô

Y

nm →

[email protected]

80 nm

-

Melamine-formaldehyde Isoper G Gelatin-Acacia

Decane

PEG gel Water Agarose gel

Particles

Ca

0 0.05 0.1 0.15 0

20

40

60

80

100

120

Dro

plet

dia

met

er (�

m)

Capillary number of the continuous phase flow

SDS 0.01 wt% SDS 0.1 wt% SDS 0.2 wt%

Elix

PVA 1.2 wt% PVA 2.4 wt%

SDS 0.5 wt% SDS 1.0 wt%

Dispersed phase

Continuous phase

100�m

Top View Side View

Generation of monodisperse emulsion droplets

300 �m

1 mm 100 �m 10 �m 1 �m 100 nm 10 nm 1 nm 1

George M Whitesides, nature biotechnology, 21, 1161 (2003)

A coakroach A human hair Polygonum pollen grain Red blood cells

An aggregate of half-shells on

palladium

Cobalt nanocrystal superlattice Aspirin molecule

500 �m 500 �m

(Exposure time 1/5 s) 200 �m

(Exposure time 1/5 s) 200 �m

W: glycerol O: Egg lecithin, Tween80, Tricaprylin, Tricaproin, Paclitaxel

Page 5: Š ï U V · 1 m dp = 320 nm CV = 12.9 % Biomass H O O O H Lactic acid n Polylactide (PLA) 0 ö ? Ê Â í º / < à Ç i É æ L Ñ S P y È%&>Ô4 )F%&>Õ>Ì %&>Ì>Ô
Page 6: Š ï U V · 1 m dp = 320 nm CV = 12.9 % Biomass H O O O H Lactic acid n Polylactide (PLA) 0 ö ? Ê Â í º / < à Ç i É æ L Ñ S P y È%&>Ô4 )F%&>Õ>Ì %&>Ì>Ô
Page 7: Š ï U V · 1 m dp = 320 nm CV = 12.9 % Biomass H O O O H Lactic acid n Polylactide (PLA) 0 ö ? Ê Â í º / < à Ç i É æ L Ñ S P y È%&>Ô4 )F%&>Õ>Ì %&>Ì>Ô
Page 8: Š ï U V · 1 m dp = 320 nm CV = 12.9 % Biomass H O O O H Lactic acid n Polylactide (PLA) 0 ö ? Ê Â í º / < à Ç i É æ L Ñ S P y È%&>Ô4 )F%&>Õ>Ì %&>Ì>Ô
Page 9: Š ï U V · 1 m dp = 320 nm CV = 12.9 % Biomass H O O O H Lactic acid n Polylactide (PLA) 0 ö ? Ê Â í º / < à Ç i É æ L Ñ S P y È%&>Ô4 )F%&>Õ>Ì %&>Ì>Ô

•••

••

Page 10: Š ï U V · 1 m dp = 320 nm CV = 12.9 % Biomass H O O O H Lactic acid n Polylactide (PLA) 0 ö ? Ê Â í º / < à Ç i É æ L Ñ S P y È%&>Ô4 )F%&>Õ>Ì %&>Ì>Ô

5

(

0 1 2 4 7 10 14 day

0 2 3 4 7 10 14 day

5 6 7

AA/�-

actin

)

mRNA variants

••••

2008-136880

PCT/JP2009-058996

086 235 7379 E-mail ouchidam md.okayama-u.ac.jp

16

14

12

10

8

6

4

2

50%

(g

)

0

Page 11: Š ï U V · 1 m dp = 320 nm CV = 12.9 % Biomass H O O O H Lactic acid n Polylactide (PLA) 0 ö ? Ê Â í º / < à Ç i É æ L Ñ S P y È%&>Ô4 )F%&>Õ>Ì %&>Ì>Ô

Biofertilizer utilizing plant-growth promoting symbiotic bacteria

Page 12: Š ï U V · 1 m dp = 320 nm CV = 12.9 % Biomass H O O O H Lactic acid n Polylactide (PLA) 0 ö ? Ê Â í º / < à Ç i É æ L Ñ S P y È%&>Ô4 )F%&>Õ>Ì %&>Ì>Ô