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OO
OO
yx
O OO
Hn
O
N
OCH
CH3CH3
NH m
ONH
Ol
O OO
Hn
(PDMA-g-PDLLA)
1 �m
dp = 320 nm CV = 12.9 %
Biomass H O O
O
H
nLactic acid Polylactide (PLA)
O/W
30
25
20
15
10
5
0Ave
rage
par
ticle
dia
met
er (�
m)
100806040200Continuous phase flow (�L/min)
30
25
20
15
10
5
0
CV
(%)
CV
NH
O
ONal
O
NH
O
O
ONaO
O
Isopropylamine modified poly(succinimide) (IPA-PSI)
IPA-PSI
Heating Cooling
1 �m
Drying up
Aqueous IPA-PSI solution
Dehydrated IPA-PSI
Protein
Hydrated IPA-PSI
Heating Particle formation
0.12
0.10
0.08
0.06 0.04
0.02
0.00 0.20 0.15 0.10 0.05 0.00
Initial concentration of proteins (wt%)
Phycocyanin Hemoglobin Cytochrome c
Enc
apsu
late
d pr
otei
n (g
/ g-
poly
mer
)
100
80
60
40
20
0 Enc
losi
ng e
ffici
ency
(%)
0.20 0.15 0.10 0.05 0.00
Initial concentration of proteins (wt%)
Phycocyanin Cytochrome c < Hemoglobin
PAspNa
PSI
PSI
PSI
PSI
PSI
50 �m
R-NH2
pH
PSIPAsp
L-Aspartic acid Poly(aspartic acid) (PAsp)
Polysuccinimide (PSI)
(�m)
0.01 1 10 0.1 100
20 �m
20 �m
20 �m
PFOB
PFOB
PLA
PFOB
; perfluorooctyl bromide (PFOB)
PLA
30 �m 20 �m
: PAspNa
400 nm
TEM SEM
http://www.process.ecm.okayama-u.ac.jp/
PLA
1 �m
Enclosing efficiency
PSI
�
Y
nm →
80 nm
-
Melamine-formaldehyde Isoper G Gelatin-Acacia
Decane
PEG gel Water Agarose gel
Particles
Ca
→
0 0.05 0.1 0.15 0
20
40
60
80
100
120
Dro
plet
dia
met
er (�
m)
Capillary number of the continuous phase flow
SDS 0.01 wt% SDS 0.1 wt% SDS 0.2 wt%
Elix
PVA 1.2 wt% PVA 2.4 wt%
SDS 0.5 wt% SDS 1.0 wt%
Dispersed phase
Continuous phase
100�m
Top View Side View
Generation of monodisperse emulsion droplets
300 �m
1 mm 100 �m 10 �m 1 �m 100 nm 10 nm 1 nm 1
George M Whitesides, nature biotechnology, 21, 1161 (2003)
A coakroach A human hair Polygonum pollen grain Red blood cells
An aggregate of half-shells on
palladium
Cobalt nanocrystal superlattice Aspirin molecule
500 �m 500 �m
(Exposure time 1/5 s) 200 �m
(Exposure time 1/5 s) 200 �m
W: glycerol O: Egg lecithin, Tween80, Tricaprylin, Tricaproin, Paclitaxel
•••
•
••
5
(
0 1 2 4 7 10 14 day
0 2 3 4 7 10 14 day
5 6 7
AA/�-
actin
)
mRNA variants
••••
2008-136880
PCT/JP2009-058996
086 235 7379 E-mail ouchidam md.okayama-u.ac.jp
16
14
12
10
8
6
4
2
50%
(g
)
0
Biofertilizer utilizing plant-growth promoting symbiotic bacteria