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The insects will tell you everything, The insects will tell you everything, people lie, but insects don't lie.” people lie, but insects don't lie.” Murray Marks ---forensic anthropologist, Murray Marks ---forensic anthropologist, University of Tennessee University of Tennessee

“ The insects will tell you everything, people lie, but insects don't lie.” Murray Marks ---forensic anthropologist, University of Tennessee

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“ “ The insects will tell you The insects will tell you everything, people lie, but insects everything, people lie, but insects

don't lie.”don't lie.”

Murray Marks ---forensic anthropologist, Murray Marks ---forensic anthropologist, University of TennesseeUniversity of Tennessee

Blowfly Life CycleBlowfly Life Cycle

The Role of Arthropods (Phylum The Role of Arthropods (Phylum Arthropoda) in Animal DecompositionArthropoda) in Animal Decomposition

The Role of Arthropods (Phylum The Role of Arthropods (Phylum Arthropoda) in Animal DecompositionArthropoda) in Animal Decomposition

Key Points:Key Points:

The Role of Arthropods (Phylum The Role of Arthropods (Phylum Arthropoda) in Animal DecompositionArthropoda) in Animal Decomposition

Key Points:Key Points:

• Following death, blow flies (Calliphoridae) and flesh flies Following death, blow flies (Calliphoridae) and flesh flies (Sarchophagidae) are the first animals to locate and colonize the (Sarchophagidae) are the first animals to locate and colonize the carcass – arriving within ten minutes of death.carcass – arriving within ten minutes of death.

(Order: DIPTERA, Family: CALLIPHORIDAE)(Order: DIPTERA, Family: CALLIPHORIDAE)

Green Bottle FlyGreen Bottle Fly

Phaenicia sericataPhaenicia sericata, and , and Phaenicia cuprinaPhaenicia cuprina

(Order: DIPTERA, Family: CALLIPHORIDAE)(Order: DIPTERA, Family: CALLIPHORIDAE)

Hairy Maggot Blow FlyHairy Maggot Blow Fly

(Chrysomya rufifacies(Chrysomya rufifacies))

(Order: DIPTERA, Family: CALLIPHORIDAE)(Order: DIPTERA, Family: CALLIPHORIDAE)

Oriental Latrine FlyOriental Latrine Fly

Chrysomya megacephalaChrysomya megacephala

(Order: DIPTERA, Family: CALLIPHORIDAE)(Order: DIPTERA, Family: CALLIPHORIDAE)

Blue Bottle FlyBlue Bottle Fly

((Calliphora vomitoriaCalliphora vomitoria))

(Order: DIPTERA, Family: CALLIPHORIDAE)(Order: DIPTERA, Family: CALLIPHORIDAE)

Black Blow FlyBlack Blow Fly

Phormia reginaPhormia regina

(Order: Diptera, Family: Sarcophagidae)(Order: Diptera, Family: Sarcophagidae)

Flesh FliesFlesh Flies

SarcophagaSarcophaga spp. spp.

(Order: Diptera, Family: Muscidae)(Order: Diptera, Family: Muscidae)

House flyHouse fly

(Musca domestica(Musca domestica))

(Order: Diptera: Family: Calliphoridae)(Order: Diptera: Family: Calliphoridae)

Secondary Screwworm FlySecondary Screwworm Fly

(Cochliomyia macellaria(Cochliomyia macellaria))

The Role of Arthropods (Phylum The Role of Arthropods (Phylum Arthropoda) in Animal DecompositionArthropoda) in Animal Decomposition

Key Points:Key Points:

The Role of Arthropods (Phylum The Role of Arthropods (Phylum Arthropoda) in Animal DecompositionArthropoda) in Animal Decomposition

Key Points:Key Points:

• Following death, blow flies (Calliphoridae) and flesh flies Following death, blow flies (Calliphoridae) and flesh flies (Sarchophagidae) are the first animals to locate and colonize the (Sarchophagidae) are the first animals to locate and colonize the carcass – arriving within ten minutes of death.carcass – arriving within ten minutes of death.

• The decay process is highly predictable with respect to: 1) the The decay process is highly predictable with respect to: 1) the associated external insect fauna and 2) the internal bacterial associated external insect fauna and 2) the internal bacterial fauna – passing through various stages of ecological succession fauna – passing through various stages of ecological succession from the “fresh” stage to the “skeletal” phase. from the “fresh” stage to the “skeletal” phase.

The Role of Arthropods (Phylum The Role of Arthropods (Phylum Arthropoda) in Animal DecompositionArthropoda) in Animal Decomposition

Key Points:Key Points:

• Following death, blow flies (Calliphoridae) and flesh flies Following death, blow flies (Calliphoridae) and flesh flies (Sarchophagidae) are the first animals to locate and colonize the (Sarchophagidae) are the first animals to locate and colonize the carcass – arriving within ten minutes of death.carcass – arriving within ten minutes of death.

• The decay process is highly predictable with respect to: 1) the The decay process is highly predictable with respect to: 1) the associated external insect fauna and 2) the internal bacterial associated external insect fauna and 2) the internal bacterial fauna – passing through various stages of ecological succession fauna – passing through various stages of ecological succession from the “fresh” stage to the “skeletal” phase. from the “fresh” stage to the “skeletal” phase.

• see http://www.deathonline.net/decomposition/index.htmsee http://www.deathonline.net/decomposition/index.htm

The Role of Arthropods (Phylum The Role of Arthropods (Phylum Arthropoda) in Animal DecompositionArthropoda) in Animal Decomposition

Key Points:Key Points:

• Following death, blow flies (Calliphoridae) and flesh flies Following death, blow flies (Calliphoridae) and flesh flies (Sarchophagidae) are the first animals to locate and colonize the (Sarchophagidae) are the first animals to locate and colonize the carcass – arriving within ten minutes of death.carcass – arriving within ten minutes of death.

• The decay process is highly predictable with respect to: 1) the The decay process is highly predictable with respect to: 1) the associated external insect fauna and 2) the internal bacterial associated external insect fauna and 2) the internal bacterial fauna – passing through various stages of ecological succession fauna – passing through various stages of ecological succession from the “fresh” stage to the “skeletal” phase. from the “fresh” stage to the “skeletal” phase.

• By studying these stages of decay and the abiotic conditions at By studying these stages of decay and the abiotic conditions at the site (temperature, habitat conditions, etc…), forensic the site (temperature, habitat conditions, etc…), forensic scientists can accurately predict the time of death or post-scientists can accurately predict the time of death or post-mortem interval (PMI). mortem interval (PMI).

The egg mass – the advantage of a well-The egg mass – the advantage of a well-placed larvaplaced larva

The egg mass – the advantage of a well-The egg mass – the advantage of a well-placed larvaplaced larva

The egg mass – the advantage of a well-The egg mass – the advantage of a well-placed larvaplaced larva

The egg mass – the advantage of a well-The egg mass – the advantage of a well-placed larvaplaced larva

The egg mass – the advantage of a well-The egg mass – the advantage of a well-placed larvaplaced larva

The egg mass – the advantage of a well-The egg mass – the advantage of a well-placed larvaplaced larva

The egg mass – the advantage of a well-The egg mass – the advantage of a well-placed larvaplaced larva

The egg mass – the advantage of a well-The egg mass – the advantage of a well-placed larvaplaced larva

The egg mass – the advantage of a well-The egg mass – the advantage of a well-placed larvaplaced larva

The egg mass – the advantage of a well-The egg mass – the advantage of a well-placed larvaplaced larva

The egg mass – the advantage of a well-The egg mass – the advantage of a well-placed larvaplaced larva

The egg mass – the advantage of a well-The egg mass – the advantage of a well-placed larvaplaced larva

The egg mass – the advantage of a well-The egg mass – the advantage of a well-placed larvaplaced larva

The egg mass – the advantage of a well-The egg mass – the advantage of a well-placed larvaplaced larva

The egg mass – the advantage of a well-The egg mass – the advantage of a well-placed larvaplaced larva

The egg mass – the advantage of a well-The egg mass – the advantage of a well-placed larvaplaced larva

The egg mass – the advantage of a well-The egg mass – the advantage of a well-placed larvaplaced larva

The Role of Dipterans (true flies) in The Role of Dipterans (true flies) in Determining Time of DeathDetermining Time of Death

• Flies are Flies are ectothermsectotherms, thus growth , thus growth rates for a particular species are rates for a particular species are primarily a function of time and primarily a function of time and temperature.temperature.

The Role of Dipterans (true flies) in The Role of Dipterans (true flies) in Determining Time of DeathDetermining Time of Death

• Flies are Flies are ectothermsectotherms, thus growth , thus growth rates for a particular species are rates for a particular species are primarily a function of time and primarily a function of time and temperature.temperature.

• If you know the temperature and the If you know the temperature and the size of the larvae (maggot), you can size of the larvae (maggot), you can determine the time of initial determine the time of initial colonization.colonization.

The Adult Fly -- FeedingThe Adult Fly -- Feeding