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MICROBE/MICROORGANISM Small living organism
Not visible to the naked eye Found everywhere Part of your normal flora Some microorganisms cause infection—
called pathogens or germs At times a microorganism that is beneficial
in one area can cause problems in another area
Microorganisms like: Warm environment Darkness Source of food and moisture
MICROORGANISMS: Bacteria
One-celled, multiply rapidlyCocci
Diplococci, Streptococci, StaphlococciBacilliSpirilla
AntibioticsSome strains of bacteria have become
antibiotic resistant Illnesses:
Strep throat, pneumonia, pertussis, tetanus, UTI, gonorrhea, syphilis
MICROORGANISMS: Protozoa
Found in decayed materials, bird feces, contaminated water, insect bites
Malaria, trichomonas, African sleeping sicknes Fungi
Plant like organisms that live on dead organic matter Yest and Molds
Athletes foot, thrush, yeast infections, ring worm
Antifungal Rikettsia
Parasitic organisms Found on fleas, lice, ticks, mice
MICROORGANISMS: Viruses
Smallest microorganismCan’t reproduce unless in another living sellSpread by body secretionsDifficult to kill
Diseases:Cold, measles, chicken pox, warts, herpes,
polio, influenza, mumps
VIRUSES Able to mutate and change genetic
information SARS
Variant of the common cold Flu like symptoms to respiratory failure and death
West Nile Virus Mosquito are the carriers Mild fevers to death
MonkeypoxEbolaH5N1 Virus (Bird Flu)
Contact with infected poultry or surfaces Death rate is 50-60%
VIRUSES IN HEALTH CARE Hepatitis B
Destroys and scars liver cells Hepatitis C
Serious liver damage Acquired immunodeficiency syndrome
(AIDS)Caused by human immunodeficiency virus
(HIV)No cure or vaccine Suppresses the immune system
Individuals are not able to fight off infections Important to take precautions
MICROORGANISMS: Helminths
Multicellular parasitic organismsTransmitted through eggs or larvae in
contaminated foods, contaminated meat or infected insects
Some can penetrate the skin to enter the body
Types:HookwormsTrichinella spiralisEntrobiasisTape worm (taenio solium)
INFECTIONS Pathogenic microorganisms cause infections
in different waysProduce toxinsCause allergic reactionsAttack and destroy living cells
Classifications:Endogenous: originated within the body
Tumors, metabolic disorders, congenital abnormalitiesExogenous: originated outside the body
Trauma, radiation, chemical agentsNonsocomial: Infections from a health care
facilityOpportunistic: infections that occur when body
defenses are weakened
CHAIN OF INFECTION Factors must exist for disease to occur and
spread Causative agent: pathogen that can cause a
disease Reservoir: area where the agent can live Portal of exit: way to escape the reservoir Mode of transportation: way for the causative
agent to be transmitted to another host Portal of entry: Way to get into the host Susceptible host: individual who can contact the
disease Ending the chain:
If any part is eliminated, the spread will be stopped
ASEPTIC TECHNIQUES Ways to maintain cleanliness and eliminate
or prevent contamination Hand washing Disposable gloves Proper cleaning of instruments and equipment Thorough cleaning of the environment
3 Levels Antisepsis: prevent or inhibit growth
Alcohol, betadine Disinfection: destroys or kills
spores and viruses may not die with disinfection Bleach solutions
Sterilization: destroys all Autoclaving
BIOTERRORISM Use of microorganisms or biological agents
as weapons CDC has identified major bioterrorism threats
Small poxAnthraxPlagueBotulismTalaremiaFilovirus
Bioterrorism act of 2002Requires development of a plan against
bioterrorism to increase security in the US
STANDARD PRECAUTIONS Blood Born pathogen Standards
1991 by OSHAMust be followed by all health care facilitiesRegulations
Develop a written exposure control plan Identify all employees who have potential exposure Provide Hep B vaccinations Provide personal protective equipment Provide hand washing supplies and areas Ensure work site is clean Dispose of infectious materials properly Label biohazard wastes and post signs Provide training
NEEDLE STICKS Safety and prevention Act
Passed in 2000CDC estimated 600,000-800,000 needle
sticks a yearRequires employees to:
Identify and use effective and safer medical devices
Incorporate changes in annual update of exposure control plan
Get input from nonmanagerial employees Maintain a sharps injury log Make sure every employee uses standard
precautions at all times to prevent contact of body fluids
HAND WASHING Major aspect of standard precautions Most important for aseptic technique Prevents spread of pathogens and
protects worker Regular Hand washing
Plain soap and water—routine cleaning Antiseptic Hand washing
Antimicrobial soap and water—invasive procedures
Antiseptic Hand rubsWaterless hand washing using alcohol-based
hand cleaners
WHEN TO WASH When you arrive and before leaving Before and after every patient contact Before and after putting on gloves If gloves rip Before and after handling a specimen
Basics Use soap Warm water Use friction to clean all surfaces Point fingertips downwards Use paper towels to dry off and shut off Clean nails
MORE INFO ABOUT HANDS Waterless hand cleaners
Proven safeRecommended when the hands are not
visibly dirty Gloves
When ever in contact with blood, body fluids, secretions, or specimens they should be worn
Change after contact with patientsWash hands after removing
Gowns/Masks/Eyeware/FaceshieldsWorn during procedures that are likely to
cause splashing or spraying
SHARPS Use care while handling sharp objects Needles
Place in a leakproof, puncture resistant container labeled with a red biohazard symbol
Surgical blades, razors, and other sharp objects must also be discarded in the sharps container
Dispose of sharps appropriately according to law
Spills or splashesWiped up immediately Gloves need to be warnDisinfect per protocol
WASTE AND SOILED LINEN DISPOSAL Wear gloves while handling waste and
soiled linen Follow policy Infectious wastes must be placed in
special biohazard bags Soiled linen should be placed in laundry
bagsGloves should be wornBags containing contaminated linen need to
be labeled Reporting cuts and injuries
Required to report
METHODS OF INFECTION CONTROL Various methods are used to achieve
infection control Different methods lead to different
levels of aseptic controlAntisepsis: prevents or inhibits growth of
pathogens, but not effective against spores and viruses
Disinfection: destroys or kills pathogenic organisms, but not always effective against spores and viruses
Sterilization: destroys all microorganisms both pathogenic and non pathogenic as well as spores and viruses
AUTOCLAVE Uses steam under pressure or gas Most efficient method of sterilization Process:
Items are washed first with soapy water to remove any residue
Items are wrapped for autoclaving Special wrapping technique to ensure no open
edges to allow contaminationAutoclave is properly loaded Indicator allows you to know when items are
sterilized—time period can varyCare of items after autoclaving
CHEMICAL DISINFECTION Chemicals used for aseptic control Disinfects, but doesn’t sterilize Used for instruments that don’t penetrate the body Preparation
Items are cleaned and debris is removedChemical solution is used
Requirements:Large enough container to fit all items Items must be separate from one anotherTight fitting lid to prevent contamination
Solution must be changed according to manufacture's instructions
ULTRASONIC UNIT Sound waves are used to produce bubbles, when the bubble strikes the item being cleaned it explodes removing the dirt and residue Aseptic Control Special cleaning solutions are used in the
unit, some are permanent solution tanks Instruments, impression trays, glass
products, jewelry can be cleaned Permanent tanks need to be drained and
cleaned according to manufacturers instructions
SUMMARY Different methods provide different
levels of aseptic control Method used depends on equipment
available and level of aseptic control required
Read manufacture's instructions before using any equipment
Make sure all items to be sterilized or disinfected are clean, rinsed, and dry before they are processed
Handle items carefully after processing to maintaining level of aseptic control
USING STERILE TECHNIQUES Many procedures require sterile
techniques to protect patient from infection
Surgical Asepsis: Procedures that keep an object or area free from living organisms
Sterile: Free from all organisms, including spores and viruses
Contaminated: organisms and pathogens are present on an article or area
Correct techniques MUST be followed to maintain sterility and prevent contamination
STERILE SUPPLIES Clean free working area
Sterile + non-sterile=unsterileSterile+ sterile=sterileSterile+ wet surface= unsterile
Handling Sterile suppliesTouching skin or clothing=unsterileBelow the waste=unsterileHold items away and in front of the body
Sterile field: area used for placement of sterile supplies
STERILE FIELD BASICS Keep the sterile field in constant view Never reach over the top of a sterile field Never turn your back to a sterile field Avoid coughing, sneezing or talking over a
sterile field Keep sterile fields clean and dry 2-inch area around sterile field =unsterile Check all items before use Use appropriate methods for removing
articlesDropMitten
STERILE GLOVES Worn while doing sterile procedures Make sure sterile tray is open and all
sterile items are ready before putting gloves on
Observe correct technique When gloves are on hands hold away
from the body and above waist Handle only sterile objects while
wearing sterile gloves Change gloves if contamination occurs
STERILE DRESSING Proper authorization Use an infectious waste bag for
contaminated dressings Wear disposable gloves while removing a
dressing Note type, color and amount of drainage
on dressings Put on sterile gloves to cleanse area and
apply new dressing Cleanse wound with proper technique If contamination occurs, start over
http://www.youtube.com/watch?v=uyNm47QKW_c&feature=related
TRANSMISSION BASED ISOLATION PRECAUTIONS Many different diseases in the healthcare
fieldSome diseases are communicable or caused by
an organism that is easily transmitted Epidemic
Occurs when the communicable disease spreads rapidly from person to person
Affects a large number of people at the same time Pandemic
Exists when the outbreak of the disease occurs over a wide geographical area
Affects high proportion of the populationTravel is a concern
TRANSMISSION BASED ISOLATION Required for communicable diseases
TuberculosisWound infectionsPertussis
Standard precautions do not eliminate the need for specific transmission based isolation precautionsTransmission based provides extra
protection against the spread of specific diseases or pathogens
TRANSMISSION ISOLATION Type of transmission-based isolation
depends on what the causative organism is and how it is transmittedPersonal Protective Equipment (PPE)
Gowns, gloves, face shields, masks
Terms:Contaminated/Dirty
Items that contain disease-producing organisms Must not be touched unless your protected
Clean Items that don’t contain organisms Effort needs to be given to prevent contamination
to clean parts
CLASSIFICATIONS OF PRECAUTIONS
Standard: Used for all patientsGloves, gowns, face masks when neededPrivate room if patient is infectious
Airborne precautions: patients with known or suspected infections transmitted by droplets in the airVaricella, tuberculosis, herpes zoster, measlesUse standard precautions as well as
respiratory precautions Private room with door closed Air in room should be filtered or discharged
outdoors Everyone must wear a respiratory filtering mask
(HEPA) Patient should not be transported from room, if
necessary mask needs to be worn
CLASSIFICATIONS OF PRECAUTIONS Droplet Precautions
Patients with known or suspected infections with pathogens transmitted by large droplet particles Influenza, meningitis, pneumonia, mumps, diphtheria
Standard precautionsPatient should be in private room or with patient of
the same infectionMask when working within 3 feet
Contact PrecautionsPatients with suspected infection with rapidly
spreading microorganisms that can be transmitted by direct or indirect contact GI diseases, respiratory, skin,wound infections,
pediculosisStandard precautionsPrivate room, gloves, gown if contactEquipment should be left in room
CLASSIFICATIONS OF PRECAUTIONS
Protective or Reverse IsolationMethod to protect patients from certain
organisms present in the environment Immunocompromised
Burns, malnourished, chemotherapy, radiation, bone marrow transplant, immune system failure
Precautions depend on patient’s condition Frequent disinfecting
Exact procedures vary depending on facilityType of units provided varySupplies or isolation equipment vary
Learn specific facility precautions, but basic principles remain the same.