O157 O157 SNP

clade 1) clade

clade 81) clade 12 O157 2)

clade O157

O157 clade 32 SNP

O157 insertion sequence IS

629 IS-printing IS 3) 2002

2009 O157 clade IS6294)

O157 IS629 clade

O157 clade

1 clade IS629

1996~2013 O157

1050 IS SNP clade

IS629

2 IS clade

1 5)

Lx clade x x clade 2 3 6 7a

7b 8a 8b 12 Ni IS i IS629

1 0 Pi, x IS i clade x IS629

RLx clade x

IS clade RLx RL2 RL3 RL6 RL7a RL7b

RL8a RL8b RL12 RLx x clade

RLx 0.75 ND

3 IS clade

1996~2010 2002~2007 O157

O157 IS

clade clade clade

CRx clade x NCx SNP clade x

O157 NPx NPND SNP clade x O157

clade x ND

O157 clade 2 3 6 7a 7b 8a 8b 12 major clade

IS629 Table 1

O157 IS clade

SNP clade Table 2

clade 3 6 8 97-100% clade

7 88% clade 12 90% Table 2 clade 2

96.6% 81.3%

clade 2 clade 3 3 clade 3 IS629

Fig. 1 clade 3

IS629 clade IS629

IS629 O157 clade

IS629 minimum spanning tree MST clade

2 3 clade 2 3 IS629

Fig. 1 clade 2 3

IS629 clade 3

clade 1 descendant ancestral clade 4/5 clade 9 putative clade 10 putative

clade 11 putative clade 13 minor clade minor clade

IS629 Table 1 minor clade

O157 IS major clade IS629 clade

major clade ND

minor clade O157 IS major clade

clade Table 3 22 clade 1 14

ND 7 clade 3 1 clade 2 46

descendant clade 4/5 10 ND 24 clade 3 12

clade 2

clade major minor clade clade

93.2% 93.4% minor clade

O157 clade

1 clade 3 descendant clade 4/5 clade 2 3 SNP 1

clade 2 3 ND O157 1

SNP clade 9

- clade

putative clade 10 11 13 4) clade O157

6)

O157 clade IS

clade O157 IS

clade

O157

IS IS clade

O157 clade

O157 IS629

O157

1) Manning, S. D. et al., Proc. Natl. Acad. Sci. USA 105: 4868-4873 (2008)

2) Etoh, Y. et al. Infect. Genet. Evol. 24: 140-145 (2014)

3) Ooka, T. et al. J. Clin. Microbiol. 47: 2888-2894 (2009)

4) Hirai, S. et al. Infect. Genet. Evol. 18: 94-99 (2013)

5) Willcox, W. R. et al. J. Gen. Microbiol. 77: 317-330 (1973)

6) Hirai, S. et al. J. Appl. Microbiol. 117: 1191-1197 (2014)

Hirai, S., Yokoyama, E., Etoh, Y., Seto, J., Ichihara, S., Suzuki, Y., Maeda, E., Sera, N., Horikawa, K.,

Sato, S., and Yamamoto, T. Putative classification of clades of enterohemorrhagic Escherichia coli

O157 using an IS-printing system. Lett. Appl. Microbiol. 61: 267-273 (2015)