Web viewThe Nature of Metabolic Pathways; Industrial Microbiological Products as Primary and Secondary Metabolites; Trophophase-idiophase Relationships in the Production of Secondary

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HEMCHANDRACHARYA NORTH GUJARAT UNIVERSITY, PATANM.SC. ( MICROBIOLOGY) CBCS Syllabus 2011

Document code : SYLLABUS MSMIC 2011Revision No: 0Name of Faculty SCIENCEFaculty Code SCProgramme Code : MSMICProgramme Name : MASTER OF SCIENCE (MICROBIOLOGY)Effective from: June 2011

2

Sem

Combination Code

Old Paper Code

Paper Code

Paper Type

Paper Name

Marking schemeCredit

Total Remarks External Internal

The

Pra

The

Pra

1 MSMIC101CC CC

Cell Biology and Genetics (CBG)

70 30 4 100

1 MSMIC102CC CC

Diversity of Life Forms and Applied Microbiology (DAM)

70 30 4 100

1 MSMIC103CC CC

Biochemistry and Analytical Techniques (BAT)

70 30 4 100

1 MSMIC101ES ES

Advanced Bioinformatics(ABI)

50 2 50Any one from MSMIC 101 ES- 106ES

1 MSMIC102ES ES

Microbial Genomics and Proteomics (MGP)


1 MSMIC103ES ES

Nanotechnology and Systems Biology (NSB)

50 2 50Any one from MSMIC 101 ES -106ES

1 MSMIC104ES ES

Environmentally Sound Technologies (EST)


1 MSMIC105ES ES

Entrepreneurship and Management for Microbiology Based Industry (EMM)

50 2 50Any one from MSMIC 101 ES - 106ES

1 MSMIC106ES ES

Microbiology of Extreme Environment (MEE)


1 MSMIC101PR PR

Practical-I (Practical based on MB 101 to 103 and selected elective paper ) (LB1)

150 6 150

3

Sem

Combination Code

Old Paper Code

Paper Code

Paper Type

Paper Name


Total Remarks Externa

l InternalThe

Pra

The

Pra

2 MSMIC201CC CC

Molecular Genetics of Bacteria and Bacteriophages(MBB)

70 30 4 100

2 MSMIC202CC CC

Research Methodology & Professional Practices(RMP)

70 30 4 100

2 MSMIC203CC CC

Bioprocess and Biochemical Engineering (BBE)

70 30 4 100

2 MSMIC201ES ES

Quality Control , Assurance and Total Quality Management(TQM)


2 MSMIC202ES ES

Microbial Physiology and Biochemistry(MPB)


2 MSMIC203ES ES

Medical Microbiology and Immunology(MMI)


2 MSMIC204ES ES

Agriculture Microbiology(AMB)


2 MSMIC205ES ES

Food and Dairy Microbiology(FDM)


4

2 MMIC206ES ES

Soil and Water Microbiology(SWM)

50 2 50Any one from MMIC 201 ES to 206

2 MSMIC201PR PR

Practical-II (Practical based on MB 201 to 203 and selected elective paper) (LB2)

150 6 150

Sem

Combination Code

Old Paper Code

Paper Code

Paper Type

Paper Name


Total Remarks Externa

l InternalThe

Pra

The

Pra

3 MSMIC301CC CC

Genetic Engineering and Protein engineering(GEP)

70 30 4 100

3 MSMIC302CC CC

Pharmaceutical Microbiology and Fermentation Technology(PFT)

70 30 4 100

3 MSMIC303CC CC

Omics, Inter Phase Microbiology and Recent Advances in Microbiology(RAM)

70 30 4 100

3 MSMIC301ES ES

Enzyme Technology(ETL) 50 2 50

Any one from MSMIC 301 ES- 305ES

3 MSMIC302ES ES

Drug Discovery and Clinical Research(DCR)


5

3 MMIC303ES ES

Microbial Production of Recombinant Molecules(MPR)


3 MSMIC304ES ES

Microbial Ecology(MEL) 50 2 50

Any one from MSMIC 301 ES- 305ES

3 MSMIC305ES ES

Contemporary Microbiology(CMB)


3 MSMIC301PR PR

Practical-III (Practical based on MB 301 to 303 and selected elective paper) (LB3)

150 6 150

6

Sem Combination Code

Old Paper Code

Paper Code Paper Type

Paper Name


Total

Remarks External Internal

The

Pra

The

Pra

4 MSMIC401MP MP

Project Work (PRW)

18

7

Paper Style

Time 3hrs Marks 100

Section IQue 1 Question from From Unit I [14]

ORQue 1 [A] Question from From Unit I [07]

[B] Question from From Unit I [07]

Que 2 Question from From Unit II [14] OR

Que 2 [A] Question from From Unit II [07][B] Question from From Unit II [07]

Que 3 Answer specifically ( Any seven ) [07][A] Question from From Unit I or Unit II[B] Question from From Unit I or Unit II[C] Question from From Unit I or Unit II[D] Question from From Unit I or Unit II[E] Question from From Unit I or Unit II[F] Question from From Unit I or Unit II[G] Question from From Unit I or Unit II[H] Question from From Unit I or Unit II

Que 4 Question from From Unit III [14]

Que 4 [A] Question from From Unit III [07][B] Question from From Unit III [07]

Que 5 Question from From Unit IV [14]OR

Que 5 [A] Question from From Unit IV [07][B] Question from From Unit IV [07]

Que 6 Answer specifically ( Any seven ) [07][A] Question from From Unit III or Unit IV[B] Question from From Unit III or Unit IV[C] Question from From Unit III or Unit IV[D] Question from From Unit III or Unit IV[E] Question from From Unit III or Unit IV[F] Question from From Unit III or Unit IV[G] Question from From Unit III or Unit IV

SAMPLE OF PRACTICAL SLIP

X

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[BASED ON TOPICS COVERED IN CBO-401]

[BACTERIOLOGY, PHYCOLOGY, MYCOLOGY AND PLANT PATHOLOGY]

Date: / / 201_ [In force from June 2011] Place:

Time: 5 Hours] [Maximum Marks: 50

Instruction: Students are requested to follow instructions given by the examiners.

1. Proceed to perform simple staining/special staining from the given sample A. Write principle of staining technique. Show your preparation to the examiner. 05

OR

1. Perform Bacterial mobility/Protozoan motility of the given water sample A by hanging drop method. Show your preparation to the examiner. 05

2. Identify and describe the structural peculiarities observed in the given specimens B, C, D and E. 16

3. Make temporary slide of the reproductive organ from the specimen F and show your preparation to the examiner. 05

4. Expose and mount the pathogen from the specimen G. Draw a labeled diagram of observed structure under microscope and show your preparation to the examiner. 05

5. Identify and describe peculiarities seen in slides H, I, and J. 06

6. (a) Journal 03

(b) Submission 05

(c) viva-voce 05

HEMCHANDRACHARYA NORTH GUJARAT UNIVERSITY, PATANProgramme code : MSMIC Programme Name : M.Sc. MICROBIOLOGY Course Code MSMIC101CC Semester : 1

CELL BIOLOGY AND GENETICS (CBG)

9

Course type : Core Compulsory Total Credit : 04Teaching time

(hours) Examination Marking scheme

Theory (hrs)

Practical (hrs)

Internal(Marks)

External(Marks)

Total(Marks)

4 X 15 = 60 30 70 ( Paper of 3 hrs) 100

Unit No.

Topic No. Content Hour

sMarks Credit

Section - I1 Cell Biology - I 15

1.1 The Origin and evolution of cell, cell theory, Cell as experimental model

1.2 Ultra-structure and function of Bacteria : Bacterial Nucleoids, Nucleosomes, Mitochondria, Microbial Cell Surfaces, Cytoplasmic Membranes, Capsules, Organs of Locomotion , Pili or Fimbriae

1.3 Major structural and functional features of eukaryotic cell: Cell membrane and transport across the cell membrane, Plasma membrane , Cell wall, Extracellular matrix and cell interaction, Nucleus, Endoplasmic reticulum, Golgi complex, Lysosome, Mitochondria, Chloroplast, Peroxisome, Plastids, Vacuoles, Cyto skeleton and Cell movement,

1.4 Cell singling : Singling at cell surface, Singling pathway

2 Cell Biology – II 2.1 Cell death and Cell renewal : Programmed cell death, Stem cell and

maintenance of adult tissue, Embryonic stem cell and therapeutic cloning

15

2.2 Eukaryotic and prokaryotic cell division and cell cycle2.3 Host Parasite Relationship : Structures and Functions involved in

Host–Parasite interaction,pathogenesis of viral diseases, pathogenesis of bacterial diseases, Microbial mechanism for escaping host defense by bacteria or pathogens

2.4 Cancer : the development and cause of cancer, Tumor viruses, Onco genes, Tumor suppressor genes, Molecular approach to cancer treatment

Section - II3 Genetics - I 15

3.1 The organization and sequence of cellular Genomes3.2 Mendel’s Principles, Concept of gene, Extensions and Modifications

of Basic Principles, Sex Determination and Sex-Linked Characteristics

3.3 Linkage and Mapping in Eukaryotes, Prokaryotes and Bacterial viruses

3.4 DNA replication in prokaryotes and eukaryotes; DNA amplification and rearrangement

4 Genetics – II 154.1 Gene transcription in prokaryotes and eukaryotes; post

transcriptional modification

10

4.2 Translation in prokaryotes and eukaryotes; post translational modifications; protein processing

4.3 Gene Expression control in Eukaryotes4.4 Population and Evolutionary Genetics, Pedigree Analysis and

Applications, Chromosome Variation

List of experiment 1 Simple staining and Negative staining, 2 Gram’s staining and Acid fast staining3 Special staining : Cell wall staining, Capsule staining, Spore staining, Flagella staining, Metachromatic

granule staining, Nucleus staining4 Polytene chromosome5 Mitosis and Meiosis6 Study of plant cell and animal cell 7 Demonstration of Bar body and drum stick8 Isolation of mitochondria9 Isolation of chloroplast 10 Isolation of DNA from plant animal and microbial cell 11 Enzyme Induction 12 Determination of G+C ratio

Reference Book :-

No Book Name 1 Introductory mycology Alexoplous2 Viruses Biswas3 Introduction to Fungi Dubey4 Virology Flint5 Virology Luria6 Microbiology Prescott7 Principles of Microbiology R. M. Atlas8 Microbiology Tortora9 Microbial life Perry10 Virology Principle and application Carter

11


Diversity of Life Forms and Applied Microbiology (DAM) Course type : Core Compulsory Total Credit : 04

Teaching time(hours) Examination Marking scheme

Theory (hrs)

Practical (hrs)

Internal(Marks)

External(Marks)

Total(Marks)

4 X 15 = 60 30 70 ( Paper of 3 hrs) 100

Unit No.


sMarks Credit

Section - I1 Concept of Biodiversity 15

1.1 Biodiversity Basics; Biodiversity: Origin, speciation and extinction and ecological role of biodiversity; Types of biodiversity: alpha, beta, and gamma diversity.

1.2 Techniques for molecular identification: Morphological methods, Biochemical, serological, Molecular methods, Fatty acid profiling, metabolic fingerprinting ( Biolog ), DGGE and TGGE

1.3 Taxonomy methods: Taxonomy and classification: Taxonomic Hierarchies; Classical approach to classification; Numerical taxonomy; Molecular taxonomy; modern methods of taxonomy and systematic.

1.4 Overview of plant and Animal biodiversity: Taxonomic criteria of classification; Classification system; Distinguishing and significant characteristics of representative group

2 Eukaryotic Microorganism and Archaebacteria2.1 Mycology : Taxonomic criteria of fungal classification; Distinguishing

and significant characteristics of Mastigomycotina, Ascomycotina, Basidiomycotina, Zygomycotina and Deuteromycotina; Fungal growth and differentiation;

15

2.2 Protozoa: Structure, classification, reproductive strategies and economic importance; Algae : structure, classification, reproductive strategies and economic importance; algal ecology ; lichens

2.3 Archaebacteria : Phenotypes of Archea : Methanogenic bacteria, extremophiles : thermophilic, halophilic bacteria; Cell structure & composition; Eco-physiology; Taxonomy & nomenclature; Salient features of representative group

2.4 Virology : Structure, classification, cultivation and economic importance of plant viruses and animal viruses; Structure, classification, cultivation and economic importance of Bacteriophage; Viroids; Prions

Section - II3 Prokaryotic Microorganismsand Viruses 15

3.1 Microbial taxonomy : Microbial evolution and diversity, Taxonomic ranks, Classification system, Major characteristics used in taxonomy,

12

Assessing Microbial Phylogeny, The Major Divisions of Life, Bergey’s Manual of Systematic Bacteriology, A Survey of Procaryotic Phylogeny and Diversity

3.2 Overview of the Deinococci and Nonproteobacteria Gram Negatives3.3 Overview Proteobacteria3.4 Overview of The Low G+C and High G+C Gram Positives bacteria

4 Applied Microbiology 154.1 Application of Microbiology and Role of Microbiologist in Dairy and

Food industry4.2 Application of Microbiology and Role of Microbiologist in Clinical and

Diagnostic field 4.3 Application of Microbiology and Role of Microbiologist in Agriculture 4.4 Application of Microbiology and Role of Microbiologist in Environment

science

List of experiment 1 Isolation and identification of bacteria belonging to Enterobacteriaceae, Bacillaceae,

PseudomonadaceaeAzotobactereceaefamily2 Isolation and identification of industrially important molds and fungi.3 Isolation of actinomyctes4 Isolation of bacteriophage5 Mitosis and Meiosis6 Study of Protozoan and algal permanent slide 7 Growth curve of E. coli and determination of growth rate and generation time, Effect of pH, temperature,

salt and glucose on growth8 Identification of Microorganism with 16S rRNA homology technique9 Microbiological analysis of clinical sample 10 Identification of bacteria by rapid kit11 Microbiological examination of food12 Microbiological examination of water13 Microbiological analysis of Air14 Microbiological analysis of Milk

Reference Book :-

No Book Name 1 Pollard, Cell Biology.2 Alberts, Molecular Biology of Cell.3 Coopper, The cell.4 Prescott, Microbiology.5 Lodish, Molecular Cell Biology.6 Griffth, An Introduction to Genetic analysis.7 Moat, Microbial Physiology.8 David White, The Physiology and Biochemistry of Prokaryotes9 Pierce Genetics A Conceptual Approach.10 Benjamin, Gene VIII

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11 Syeer and Champness. Molecular genetics of bacteria12 Nancy, Fundamental Bacterial Genetics13 Tamarin, Principles of Genetics.14 J. D. Watson, Molecular Biology of the Genes.15 Prescott, Microbiology16 Griffith, Genetic Analysis

14


BIOCHEMISTRY AND ANALYTICAL TECHNIQUES (BAT)Course type : Core Compulsory Total Credit : 04


Theory (hrs)

Practical (hrs)

Internal(Marks)

External(Marks)

Total(Marks)

4 X 15 = 60 30 70 ( Paper of 3 hrs) 100

Unit No.


sMarks Credit

Section - I1 Biochemistry - I 15

1.1 Basics of Chemistry for biologist 1.2 Protein Structure and Function : Amino Acids,Structureof Proteins,

enzyme 1.3 Intermediary Metabolism: Bioenergetics and Oxidative

Phosphorylation, Introduction to Carbohydrates, Glycolysis, TCA Cycle, Gluconeogenesis, Glycogen Metabolism, Metabolism of Monosaccharides and Disaccharides, PPP Pathway and NADPH, Glycosaminoglycans and Glycoproteins and systematic.

1.4 Lipid Metabolism: Metabolism of Dietary Lipids, Fatty Acid and Triacylglycerol Metabolism, Complex Lipid Metabolism, Cholesterol and Steroid Metabolism

2 Biochemistry - II2.1 Nitrogen Metabolism: Amino Acids: Disposal of Nitrogen, Amino Acid

Degradation and Synthesis, : Conversion of Amino Acids to Specialized Products

15

2.2 Integration of Metabolism : Metabolic Effects of Insulin and Glucagon, The Feed/Fast Cycle, Diabetes Mellitus, Obesity, Nutrition, Vitamins

2.3 Nucleotide metabolism:Characteristics and types of Nucleic acids, Biosynthesis and catabolism of purines and pyrimidines,

2.4 Molecular biology techniques: Principle and technique of nucleic acid hybridization and cot curve; sequencing of proteins and nucleic acids; Southern , Northern and South-Western blotting techniques; Polymerase Chain Reaction; Methods for measuring nucleic acid and protein interactions

Section - II3 Analytical Technique - I 15

3.1 Electrochemistry: pH and buffers, Potentiometric and Conductometric titration, Agarose electrophoresis, Native PAGE, SDS PAGE, isoelectric focusing (IEF), two dimensional gel electrophoresis, gels

15

3.2 Purification and Identification of Bio molecules : Introduction to Chromatography, Column Chromatography, Ion-Exchange Chromatography, Gel Exclusion Chromatography, Affinity Chromatography and Immunoadsorption, Perfusion Chromatography, GC. HPLC, FPLC.

3.3 Histochemical and immunotechniques: Antibody generation, detection of molecules using ELISA, RIA, western blot, immunoprecipitation, flowcytometry and immunofluorescence microscopy, detection of molecules in living cells, in situ localization by techniques such as FISH and GISH.

3.4 Radiolabeling techniques: Properties of different types of radioisotopes normally used in biology, their detection and measurement,Autoradiography

4 Analytical Technique – II 154.1 Biophysical methods: Analysis of biomolecules using UV/visible, IR,

Fluorescence, CD, ESR spectroscopy, structure determination using X-ray diffraction and NMR; analysis using light scattering, different types of mass spectrometry and surface plasma resonance methods, MALDI TOF, Atomic spectroscopy

4.2 Microscopic techniques: Light, Phase Contrast, Fuorescence, Scanning and Transmission Electron Microscopy and other Advanced Microscopy

4.3 Electrophysiological methods: Single neuron recording, patch-clamp recording, ECG, Brain activity recording, lesion and stimulation of brain, pharmacological testing, PET, MRI, fMRI, CAT.

4.4 Centrifugation technique, Biosensors

List of experiment 1 Estimation of carbohydrates ( Coles, DNS and Anthron method)2 Estimation proteins ( Folin, Bradford and Biurates)3 Estimation of lipid4 Estimation of inorganic phosphorus5 Estimation of inorganic nitrogen6 Determination of Km and V max for Enzyme7 Enzyme inhibition study8 Identification of Microorganism with 16S rRNA homology technique9 SDS PAGE analysis for Protein10 2D gel electrophoresis technique ( Demonstration)11 Determination of pK value12 Separation of amino acid by paper chromatography13 Separation of sugars/ fatty acid by thin layer chromatography14 Determination of molecular weight of protein by Column chromatography

Reference Book :-

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No Book Name 1 Lehninger, Principles of Biochemistry . 2 Lippincotts, Illustrated Reviews Biochemistry3 David Holme, Analytical Biochemistry4 Rodney Boyer, Modern Experimental Biochemistry5 Hames and Hooper, Instant Notes in Biochemistry6 Wilson and Walker, Principles and Techniques of Biochemistry and Molecular Biology7 Debnath, Tools And Techniques Of Biotechnology8 Voet and Voet, Bichemister

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HEMCHANDRACHARYA NORTH GUJARAT UNIVERSITY, PATANProgramme code : MSMIC Programme Name : M.Sc. MICROBIOLOGY Course Code MSMIC101ES Semester : 1

ADVANCED BIOINFORMATICS (ABI)Course type : Elective Subject Total Credit : 02


Theory (hrs)

Practical (hrs)

Internal(Marks)

External(Marks)

Total(Marks)

4 X 15 = 60 50 ( Paper of 3 hrs) 50


MICROBIAL GENOMICS AND PROTEOMICS (MGP)Course type : Elective Subject Total Credit : 02


Theory (hrs)

Practical (hrs)

Internal(Marks)

External(Marks)

Total(Marks)

4 X 15 = 60 50 ( Paper of 3 hrs) 50


NANOTECHNOLOGY AND SYSTEMS BIOLOGY (NSB)Course type : Elective Subject Total Credit : 02


Theory (hrs)

Practical (hrs)

Internal(Marks)

External(Marks)

Total(Marks)

4 X 15 = 60 50 ( Paper of 3 hrs) 50

18


ENVIRONMENTALLY SOUND TECHNOLOGIES (EST)Course type : Elective Subject Total Credit : 02


Theory (hrs)

Practical (hrs)

Internal(Marks)

External(Marks)

Total(Marks)

4 X 15 = 60 50 ( Paper of 3 hrs) 50


ENTREPRENEURSHIP AND MANAGEMENT FOR MICROBIOLOGY BASED INDUSTRY (EMM)Course type : Elective Subject Total Credit : 02


Theory (hrs)

Practical (hrs)

Internal(Marks)

External(Marks)

Total(Marks)

4 X 15 = 60 50 ( Paper of 3 hrs) 50


MICROBIOLOGY OF EXTREME ENVIRONMENT (MEE)Course type : Elective Subject Total Credit : 02


Theory (hrs)

Practical (hrs)

Internal(Marks)

External(Marks)

Total(Marks)

4 X 15 = 60 50 ( Paper of 3 hrs) 50

HEMCHANDRACHARYA NORTH GUJARAT UNIVERSITY, PATAN

19

Programme code : MSMIC Programme Name : M.Sc. MICROBIOLOGY Course Code MSMIC101PR Semester : 1

PRACTICAL – I (PRACTICAL BASED ON MB 101 TO 103 AND SELECTED ELECTIVE PAPER) (LB1)

Course type : Practical Total Credit : 06Teaching time


Theory (hrs)

Practical (hrs)

Internal(Marks)

External(Marks)

Total(Marks)

4 X 15 = 60 150 ( Paper of 3 hrs) 150

20


MOLECULAR GENETICS OF BACTERIA AND BACTERIOPHAGES(MBB)Course type : Core Compulsory Total Credit : 04


Theory (hrs)

Practical (hrs)

Internal(Marks)

External(Marks)

Total(Marks)

4 X 15 = 60 30 70 ( Paper of 3 hrs) 100

Unit No.


sMarks Credit

Section - II1 DNA Damage, Repair, Recombination and Gene Regulation 15

1.1 Mutations and Mutagenesis: Biochemical Basis of Mutation, Spontaneous Mutation, Fluctuation Test, Useful Phenotypes in bacterial genetics, Mutation Rate, Expression and Selection of Mutant Cell, Types of Mutation, Mutagens, Reversion and suppression of mutation

1.2 DNA Repair Evidences of DNA Repair, Specific repair pathway, General repair mechanisms, Repair pathway in E. coli, Bacteria phage repair pathway

1.3 Recombination: Overview of recombination, Molecular Model Recombination, Molecular basis for recombination in E. coli, Phage recombination , illegimate recombination, Genetic and Functional Analysis of Recombination

1.4 Regulation of gene expression: Overview of gene regulation, Transcriptional regulation in bacteria, Negative regulation, Positive regulation, Regulation by attenuation, Feedback inhibition, Translational control.

2 Plasmid, Transposition and Conjugation 2.1 Plasmid Molecular Biology: What is plasmid, Types and properties

of plasmid: Replication, Control of Copy Number, Partitioning, Incompatibility, Maintaining Plasmid belonging to the same incompatibility group. Conjugal Functions, Pilus Production, Transfer DNA Replication

15

2.2 Properties of Particular Plasmids :Major Chromosome-Mobilizing other than F, Bacteriocins, Resistance Plasmids, Agrobacatrium

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plasmid Ti, Broad Host Range Plasmids 2.3 Conjugation: Discovery of Conjugation, Mechanism of DNA transfer

during conjugation in Gram Negative Bacteria, Chromosome transfer by Plasmid, Genetic mapping with Hfr crosses, Chromosome mobilization, Prime factors, Transfer systems of gram Positive bacteria.

2.4 Transposition and site specific recombination : Insertion sequences, Detection of transposition in bacteria, Types of bacterial Transposon, Transposition, Transposon Mutagenesis, Site specific recombination, Phage Mu

Section - II3 Bacteriophage Genetics and Transformation 15

3.1 Genetics of intemperate bacteriophages:T4 Bacteriophage as a Model Genetic System,Morphology and Composition, Experimental Methods Used to Study Phage Infection, Genetic Organization of T4, DNA Replication, Molecular Biology of T4 Phage Infection, Morphogenesis and Maturation;Properties of some other common intemperate bacteriophages.

3.2 Genetics of Temperate Bacteriophages:Overview of various temperate bacteriophages, General Nature of the Temperate Response, Bacteriophage Lambda as the Archetypal Temperate Phage, Genetic Map of Lambda. Lytic Cycle, Lysogeny Cycle, Lysogenic phage and bacatrial pathogenesis.

3.3 Transduction: Bacteriophage Lambda: A Specialized Transducing Phage, Production of Transducing Particles, Physiology and Genetic Consequences of Transduction, Specialized Transducing Phages Other than Lambda, Generalized Transduction, Cotransduction.

3.4 Genetic Transformation:Standard Genetic Transformation Systems¸ Discovery of Genetic Transformation, Competent Cells, DNA Uptake and Entry, Establishment of Donor DNA in the Recipient Cell, Other Transformation Systems, Transfection, Genetic Mapping Using Transformation

4 Advances in Microbial Genetics 154.1 Bacterial and Bacteriophage Evolution : What Is Evolution,

Expression of Evolutionary Relationships, Specific Examples of Evolution, Genetic Structure of the Chromosome, Bacteria, Bacteriophage

4.2 Advanced Regulatory Topics : Global Regulatory Mechanism: Catabolic sensitive Operon, Regulation of Nitrogen assimilation, Porin synthesis, Heat shock protein, Virulence genes, Endospore formation, Ribosome and t RNA synthesis; Two component regulation system; Inteins, Small RNA Molecules

4.3 Strain Construction: Construction of Bacterial strains, Operon and Gene fusion, Construction of Phage Mutants

4.4 Genetic Methods for Investigating Bacteria: Metabolic pathways,

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Microbial physiology¸ Bacterial virulence, Specific mutagenesis, Taxonomy, evolution and epidemiology

List of experiment 1 Ultraviolet irradiation survival curve2 Fluctuation test, rapid - plate technology.3 Isolation of auxotrophic mutant4 Isolation Respiratory deficient mutant5 Isolation of temperature sensitive mutant 6 Isolation of streptomycin resistant mutant by gradient plate technique7 Ames test8 Conjugation in E. coli9 Transduction10 Plasmid curring11 Phage titration

Reference Book :-

No Book Name 1 Fundamental Bacterial genetics Trun and Trempy2 Molecular genetics of bacteria Syndeer and Champness3 Microbial genetics. Maloy4 Bacterialand Bacteriophage Genetics. Edward5 Molecular Genetics of Bacteria. Dale6 Molecular Genetics of Bacteria. Streips7 Principles of Molecular Virology Alan

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RESEARCH METHODOLOGY & PROFESSIONAL PRACTICES (RMP)Course type : Core Compulsory Total Credit : 04


Theory (hrs)

Practical (hrs)

Internal(Marks)

External(Marks)

Total(Marks)

4 X 15 = 60 30 70 ( Paper of 3 hrs) 100

Unit No.


sMarks Credit

Section - II1 Introduction to Scientific Research 15

1.1 Characteristics and types of scientific research, Basics of research methodology, Research and Experimental design, Method of Data collection

1.2 Scientific Deliveries and Communications: Writing Research proposal, Paper, Thesis, Report and Citations, Presenting scientific research: Power point presentations, Posters, Flyers, etc.

1.3 Publication processes, Review Processes and Significance of scientific communications

1.4 Study of Critical review and scientific Paper in Microbiology

2 Statistical Methods and Data analysis2.1 Definition and scope, Organizing a statistical survey and presentation

of statistically analyzed information, Basic statistical methods: Measures of central tendency, dispersion and standard error; Probability distributions: binomial, poisson and normal distribution

15

2.2 Statistical significance: Hypothesis testing, types of error, level of significance, Student’s t test, F test and Chi square goodness of fit

2.3 Comparing Parametric and Non parametric statistics, Rank test, F-max test, Mann –Whitney (U) test, and Sign test, Applications of non parametric statistics in biological research

2.4 Simple linear regression and correlation analysis,

Section - II3 Computer Science and Bioinformatics 15

3.1 Basic computing: MS Office ®, Internet, , Use of computers in statistical analysis, Database and Data base management system, Biological database : Sequence, Structure and classification

3.2 Sequence Analysis : concepts of sequence similarity, identity and homology, Global and Local alignment, Scoring matrices, BLAST, FASTA

3.3 Multiple Sequence Alignments (MSA): The need for MSA, basic

24

concepts of various approaches for MSA (e.g. progressive, hierarchical etc.); Introduction to CLUSTALW and PileUp ; concept of dendrogram and its interpretation.

3.4 Application of Bioinformatics: Gene finding, PCR Primer designing, Microbial identification, Comparative genomics, Secondary and tertiary protein structure prediction

4 Professional Practices 154.1 Concept of Quality Control and Assurance in life science research

and industry, Concept of GMP, GLP, ISO, WHO 4.2 The Business of biotechnology: Sciences and Business,

Biotechnology company fundamentals, funding, research development and marketing

4.3 Legal and regulatory issues for Biotechnology based business 4.4 Survey on Microbiology and Biotech Industry in State and Nation

List of experiment 1 Standard operation procedure and validation of autoclave, pH meter, UV visible spectrophotometer and

laminar air flow2 Computation of Mean, Mode and Median3 Computation of Standard deviation and Co-efficient of variation4 Calculation of confidence limit for the population mean.5 Student's 't' test. (Paired and unpaired)6 ANOVA.7 Regression and correlation analysis8 Chi square goodness of fit9 perform non parametric tests10 Application statistical software ( SPSS/ minitab/ metlab)11 Use of Word, Excel, Power Point, Access and internet12 Submission of scientific Review in relevant topics 13 Internet gene bank search and SRS14 BLAST and FASTA15 Local Global and Multiple sequence alignment 16 Protein structure visualization17 Primer designing

Reference Book :-

No Book Name 1 Building Biotechnology Yali Friedman2 ISO 9000 Quality Systems Handbook David Hoyle3 Handbook of Microbiological Quality Control Denyer4 Mapping your future: Exploring Careers In biomanufacturing The North Carolina Association for Biomedical

Research5 Introductory Biostatistics Chap6 Biostatistical Analysis. Zar

25

7 Developing Bioinformatics computer skill Gibas8 Bioinformatics Principle and application Ghosh9 Applied Bioinformatics Selzer10 Bioinformatics Baxevanis11 Bioinformatics for dummies Claverie12 Bioinformatics: sequence and genome analysis Moun13 Bioinformatics, Gene, Proteins and Computers. Oren14 Bioinformatics Rastogi15 Instant notes on Bioinformatics, Twyman16 Essential Bioinformatics Jin

26


BIOPROCESS AND BIOCHEMICAL ENGINEERING (BBE)Course type : Core Compulsory Total Credit : 04


Theory (hrs)

Practical (hrs)

Internal(Marks)

External(Marks)

Total(Marks)

4 X 15 = 60 30 70 ( Paper of 3 hrs) 100

Unit No.


sMarks Credit

Section - II1 Bioprocess Microbiology – I 15

1.1 Introduction: Scope of Biotechnology and Industrial Microbiology : Nature of Biotechnology and Industrial Microbiology; Characteristics of Industrial Microbiology; Patents and Intellectual Property Rights in Industrial Microbiology and Biotechnology; The Use of the Word ‘Fermentation’ in Industrial Microbiology; Organizational Set-up in an Industrial Microbiology Establishment

1.2 Screening for Productive Strains and Strain Improvement in Biotechnological Organisms: Sources of Microorganisms used in Biotechnology; Selection from naturally occurring variants; Manipulation of the genome of industrial organisms in strain improvement

1.3 Metabolic Pathways for the Biosynthesis of Industrial Microbiology Products: The Nature of Metabolic Pathways; Industrial Microbiological Products as Primary and Secondary Metabolites; Trophophase-idiophase Relationships in the Production of Secondary Products; Role of Secondary Metabolites in the Physiology of Organisms Producing Them; Pathways for the Synthesis of Primary and Secondary Metabolites of Industrial Importance; Carbon Pathways for the Formation of Some Industrial Products Derived from Primary Metabolism; Carbon Pathways for the Formation of Some Products of Microbial Secondary Metabolism of Industrial Importance.

1.4 Overproduction of Metabolites of Industrial Microorganisms: Mechanisms Enabling Microorganisms to Avoid Overproduction of Primary Metabolic Products Through Enzyme Regulation; Derangement or Bypassing of Regulatory Mechanisms for the Over-production of Primary Metabolites; Regulation of Overproduction in Secondary Metabolites; Empirical Methods Employed to Disorganize Regulatory Mechanisms in Secondary Metabolite Production

2 Bioprocess Microbiology – II2.1 Growth Kinetics : Introduction; Kinetics of batch culture;

Disadvantages of batch culture ; Advantages of continuous culture ; Growth kinetics for continuous culture; Material balance for CSTR: Rate of product formation, Growth kinetics, biomass and product

15

27

yields, YX/S and YP/S, Biomass balances (cells) in a bioreactor, Material balance in terms of substrate in a chemostat, Modified chemostat, Fed batch culture

2.2 Industrial Media and the Nutrition of Industrial Organisms : The Basic Nutrient Requirements of Industrial Media; Criteria for the Choice of Raw Materials Used in Industrial Media; Some Raw Materials Used in Compounding Industrial media; Growth Factors; Water; Some Potential Sources of Components of Industrial media, Carbohydrate sources, Protein sources; The use of plant waste materials in Industrial Microbiology media: Saccharification of Polysaccharides, Starch, Cellulose, hemi-celluloses and lignin in plant materials

2.3 Sterility in Industrial Microbiology : Introduction; The basis of loss by contaminants; Physical and Chemical Methods of Achieving Sterility : Hot plates; High temperature sterilization; Dry heat sterilization; Sterilisation with filtration; Microwave sterilization; Electron beam sterilization; Chemical sterilization; Batch sterilization; Continuous sterilization; The sterilization of the fermentor and its accessories; Media sterilization; Viruses (Phages) in Industrial Microbiology

2.4 Bioprocess Scale-up: Introduction; Scale-up procedure from laboratory scale to plant scale( Scale-up for constant KLa, for shear forces, constant mixing time); Bioreactor design criteria ; CSTR chemostat versus tubular plug flow; Dynamic model and oxygen transfer rate in activated sludge; Aerobic wastewater treatment; Fermentation economics

Section - II3 Biochemical Engineering 15

3.1 Bioreactor : Introduction; Background; Bioreactor for batch type fermentation : The Aerated Stirred Tank, Anerobic Batch, Airlift bioreactors, Bubble column, Surface or Solid State; Bioreactor Configurations for Fed-batch Cultivation and Continuous fermentations; Calculation for bioreactor: Heat transfer; Design equations for CSTR fermenter; Monod model for a chemostat ; Temperature effect on rate constant; Scale-up of stirred-tank bioreactor

3.2 Dissolved Oxygen Measurement and Mixing: Introduction; Measurement of dissolved oxygen concentrations; Oxygen transfer rate (OTR); Respiration quotient (RQ); Agitation rate studies ; Gas and Liquid System (Aeration and Agitation): Introduction; Aeration and agitation; Effect of agitation on dissolved oxygen; Air sparger; Oxygen transfer rate in a fermenter; Mass transfer in a gas–liquid system; Gas hold-up; Agitated system and mixing phenomena; Characterisation of agitation; Types of agitator; Gas–liquid phase mass transfer (Oxygen transport, Diameter of gas bubble formed D0 )

3.3 Material and Elemental Balance: Introduction; Growth of stoichiometry and elemental balances; Energy balance with example of continuous ethanol fermentation; Conservation of mass principle with example of Acetic acid fermentation process

3.4 Fermentation Process Control: Introduction; Bioreactor controlling probes; Characteristics of bioreactor sensors; Temperature measurement and control; DO measurement and control; pH/Redox

28

measurement and control; Detection and prevention of the foam; Biosensors

4 Downstream Processing, Scale up and Economics 154.1 Extraction of Fermentation Products: Solids (Insolubles) Removal:

Filtration, Centrifugation, Coagulation and flocculation, Foam fractionation, Whole-broth treatment; Primary Product Isolation: Cell disruption, Liquid extraction (Continuous extraction column process, rotating disk contactors), Dissociation extraction, Adsorption ( Ion-exchange, Langmuir isotherm, Freundlich isotherm and Fixed-bed), Precipitation; Purification: Chromatography, Carbon decolorization, Crystallization; Product Isolation: Crystalline processing, Drying.

4.2 Membrane Separation Processes: Introduction; Types of membrane; Membrane processes; Nature of synthetic membranes; General membrane equation; Cross-flow microfiltration; Ultrafiltration; Reverse osmosis; Membrane modules.

4.3 Advanced Downstream Processing in Biotechnology: Introduction; Protein products; Cell disruption; Protein purification; General problems associated with conventional techniques; Fluidised bed adsorption; Design and operation of liquid fluidised beds; Interfaced and integrated fluidised bed/expanded bed system.

4.4 Immobilized Enzymes and Immobilized Cells: Advantages of immobilized biocatalysts in general; Methods of immobilizing enzymes; Methods for the immobilization of cells; Practical Application of Immobilized Biological Catalyst Systems; Bioreactors Designs for Usage in Biocatalysis ; Immobilised cell reactor(ICR) experiments and ICR rate model.

List of experiment 1 Amylase Production: Screening, Optimization, production and Purification (affinity chromatography)2 Protease Production: Screening, Optimization, Production and Purification (Salt precipitation, ion exchange

chromatography)3 Immobilization of enzyme 4 Estimation aeration efficiency5 Reverse micelle formation 6 Screening of Organic acid producer microorganism7 Screening of Antibiotic producer microorganism 8 Operation of fermentation 9 Sterility testing

Reference Book :-

No Book Name

1 Modern Industrial Microbiology and biotechnology. Okafor2 Biochemical Engineering and Biotechnology. Najafpour3 Biochemical engineering. Shigeo4 Principles of fermentation technology. Whittaker5 Microbial Biotechnology. Alexander

29

6 Techniques in Applied Microbiology Sikyta7 Fermentation and Biochemical Engineering Handbook. Vogel8 Practical Fermentation Technology. Mcneil9 Bioprocess engineering Principle. Doran10 Modern Biotechnology. Nathan12 Fermentation microbiology and Biotechnology, Taylor and Francis Mansi13 Industrial Microbiology: An Introduction, Blackwell publication Waites14 Bioprocess Engineering Basic Concept, Prentica Hall of India Michal15 A text book of Industrial microbiology. Crueger16 Microbial Fundamentals of Biotechnology Volkmar17 Microbial technology: fermentation technolog Peppler

30


QUALITY CONTROL , ASSURANCE AND TOTAL QUALITY MANAGEMENT (TQM)Course type : Elective Subject Total Credit : 02


Theory (hrs)

Practical (hrs)

Internal(Marks)

External(Marks)

Total(Marks)

4 X 15 = 60 50 ( Paper of 3 hrs) 50


MICROBIAL PHYSIOLOGY AND BIOCHEMISTRY (MPB)Course type : Elective Subject Total Credit : 02


Theory (hrs)

Practical (hrs)

Internal(Marks)

External(Marks)

Total(Marks)

4 X 15 = 60 50 ( Paper of 3 hrs) 50


MEDICAL MICROBIOLOGY AND IMMUNOLOGY (MMI)Course type : Elective Subject Total Credit : 02


Theory (hrs)

Practical (hrs)

Internal(Marks)

External(Marks)

Total(Marks)

4 X 15 = 60 50 ( Paper of 3 hrs) 50

31


AGRICULTURE MICROBIOLOGY (AMB) Course type : Elective Subject Total Credit : 02


Theory (hrs)

Practical (hrs)

Internal(Marks)

External(Marks)

Total(Marks)

4 X 15 = 60 50 ( Paper of 3 hrs) 50


FOOD AND DAIRY MICROBIOLOGY (FDM)Course type : Elective Subject Total Credit : 02


Theory (hrs)

Practical (hrs)

Internal(Marks)

External(Marks)

Total(Marks)

4 X 15 = 60 50 ( Paper of 3 hrs) 50


SOIL AND WATER MICROBIOLOGY (SWM)Course type : Elective Subject Total Credit : 02


Theory (hrs)

Practical (hrs)

Internal(Marks)

External(Marks)

Total(Marks)

4 X 15 = 60 50 ( Paper of 3 hrs) 50

32

HEMCHANDRACHARYA NORTH GUJARAT UNIVERSITY, PATANProgramme code : MSMIC Programme Name : M.Sc. MICROBIOLOGY Course Code MSMIC201PR Semester : 2

PRACTICAL – II (PRACTICAL BASED ON MB 201 TO 203 AND SELECTED ELECTIVE PAPER) (LB2)



Theory (hrs)

Practical (hrs)

Internal(Marks)

External(Marks)

Total(Marks)

4 X 15 = 60 150 ( Paper of 3 hrs) 150

33


GENETIC ENGINEERING AND PROTEIN ENGINEERING (GEP)Course type : Core Compulsory Total Credit : 04


Theory (hrs)

Practical (hrs)

Internal(Marks)

External(Marks)

Total(Marks)

4 X 15 = 60 30 70 ( Paper of 3 hrs) 100

Unit No.


sMarks Credit

Section - II1 Concepts and tools and technique for genetic engineering 15

1.1 Concepts and application: Introduction to gene cloning; Application of Recombinant microorganisms; Application of transgenic Plant Technology; Application of animal cloning and Transgenic animal technology.

1.2 Enzyme used in genetic engineering: Restriction endonuclease; DNA Polymerase: Reverse transcriptase; RNA polymerase; Alkaline Phosphatase; Polynucleotide Kinase; DNA ligase; Deoxyribonuclease; Ribonuclease; Phosphodiesterase; β Agarase; Uracil – DNA Glycoylase; Proteinase K; Lysosome; Topoisomerase

1.3 Cutting of DNA: Host Controlled Restriction Modification system; Nomenclature of Restriction Endonuclease; Types of Restriction Endonuclease; Recognition sites; Cleavage by Restriction endonuclease; variants of Restriction Endonuclease; Application of Restriction Endonuclease.

1.4 Joining of DNA Fragments: Introduction; Ligation of DNA fragment using DNA ligase; ligation using homopolymer Tailing; Increasing versatility and Efficiency of ligation by modification of the Ends of Restriction Fragments; Ligation of PCR products.

2 Vectors 2.1 Plasmid as a vector: pSC101; pSF124; Col E1; pBR 322 series;

pUCSeries; pGEM series; pET,pBAD15

2.2 Bacteriophage as a vector:lambda phage; M13; Cosmid;Phagemids; Phasmids;Fosmid;

2.3 Advanced vector: Shuttle vector; Expression vector; Advanced gene trapping vector; Specialized vector for making SS DNA; facilitate Purification of cloned product; promotes solubilization of expressed product; promotes export of cloned product; PAC, YAC, BAC, HAC;

2.4 Other vectors : Chimeric vector; Gram negative bacteria other than E. coli as cloning vector; Gram positive bacteria as cloning vector; Plant and Animal Vectors; Fungi system other than yeast.

34

Section - II3 15

3.1 Introduction of DNA in to Host : Introduction; Introduction of DNA in to bacterial cells; Introduction of DNA in to yeast cells; Genetic transformation of Plants; Introduction DNA in to insect

3.2 Construction of Genomic and c DNA Libraries: Introduction; Genomic Library ; cDNA Library; PCR as an alternative to library Construction; Functional cloning; Positional cloning; Differential cloning

3.3 Techniques for Selection, Screening and characterization of trans formants: Introduction; Selectable Marker gene; Reporter genes; Screening of clone(s) of interest; Nucleic Acid Blotting and Hybridization; Protein structure/ Function Fusion- based techniques

3.4 Safety regulation related to genetic engineering: Introduction; National regulatory Mechanism for implementation of biosafety guideline for handling GMOs; Salient features , revised Guidelines for Research in transgenic Plant and risk assessment; regulation of Gm Plant; Regulation of steam Cell research and human cloning; Patenting for Molecular Biotechnology; Ethical issues

4 154.1 Site directed mutagenesis; Concept tools, technique of and

application 4.2 Concept of protein engineering; Evolutionary Methods for Protein

Engineering; Phage Display Systems for Protein Engineering; Cell Surface Display Systems for Protein Engineering; Cell-Free Display Systems for Protein Engineering.

4.3 Protein engineering in basic and applied biotechnology; Enhanced recovery and folding of recombinant proteins using Fusion protein strategies; Protein engineering for affinity purification; Stabilization of industrial enzymes by protein engineering; Engineering of Therapeutic Proteins.

4.4 DNA Microarray technology: Concepts, tools and techniques, data generation and analysis, application; Microarrays for Bacterial Typing; Overview of protein Microarray technology .

List of experiment 1 Isolation of genomic DNA from Bacteria2 Isolation of genomic DNA from Plant3 Isolation of genomic DNA from Blood4 Isolation of genomic from fungi5 Agarose gel electrophoresis and recovery of DNA from gel 6 Isolation of plasmid7 RFLP8 RAPD9 PCR amplification

35

10 Cloning in bacteria11 Transformation of plants 12 Protein denaturation and in vitro Protein folding13 BT cotton testing

Reference Book :-No Book Name 1 An Introduction to Genetic Engineering Nicholl2 Analysis of Genes and Genomes Reece3 Principle of gene Manipulation Primrose4 Gene cloning and DNA Analysis Brown5 Gene Cloning and Manipulation Howe6 The ABC of gene cloning Wong7 Recombinant DNA genes and genomics Watson8 Engineering the Genetic Code Budisa9 Protein Engineering and Design Sheldon J. Park10 Enzyme Functionality Design, Engineering, and Screening Allan Svendsen11 Protein engineering in industrial Biotechnology by Lilia Alberghina Lilia Alberghina12 Protein Arrays, Biochips, and Proteomics The Next Phase of Genomic Joanna S. Albala13 Discovery by Microarrays for an Integrative Genomics Isaac

36


PHARMACEUTICAL MICROBIOLOGY AND FERMENTATION TECHNOLOGY (PFT)Course type : Core Compulsory Total Credit : 04


Theory (hrs)

Practical (hrs)

Internal(Marks)

External(Marks)

Total(Marks)

4 X 15 = 60 30 70 ( Paper of 3 hrs) 100

Unit No.


sMarks Credit

Section - II1 Pharmaceutical Microbiology 15

1.1 Microbial aspect of Pharmaceutical processing : Microbial spoilage and prevention of pharmaceutical product; Principle and practice of sterilization, Sterile pharmaceutical product , Factory and hospital hygeny and good Manufacturing practice.

1.2 Drug Discovery: Targets and Receptors; Drug Discovery: Small Molecule Drugs; Drug Discovery: Large Molecule Drugs: Enzymes, Vaccines (Attenuated, DNA, Subunit, vector), Antibodies( Monoclonal antibodies) , Cytokines, Hormones, Gene Therapy, Stem Cells;

1.3 Drug Development and Preclinical Studies; Clinical Trial : Overview, Role of microbiologist in CRO;

1.4 Bio safety: Principle of bio safety , Laboratory Bio safety Level Criteria; Bioterrorism; Biomedical waste management

2 Novel Microbial products2.1 Probiotics and Prebiotic : Concept, methods and application . 152.2 Production of Antibiotics and Anti-Tumor Agents: Classification and

Nomenclature of Antibiotics; General production methods; Penicillin, Cephalosporins, Tetrcycline, Bacitracin production; Problem of Antibiotic Resistance; The Search for New Antibiotics; Combating Resistance and Expanding the Effectiveness of Existing Antibiotics; Anti-Tumor Antibiotics; Newer Methods for Searching for Antibiotic and Anti-tumor Drugs; Synthesis of commercial products by recombinant microorganism: Small Biological molecules, Antibiotics, Biopolymers;

2.3 Biocatalysis in Organic Chemistry: Nature and Use of Steroids and Sterols, Uses of Steroids and Sterols, Types of microbial transformations in steroids and sterols, Fermentation conditions used in steroid transformation, Asymmetric Catalysis in the Pharmaceutical and Agrochemical Industries

2.4 Production of Microbial Insecticides: Alternatives to Chemical Insecticides; Biological Control of Insects; Bacillus thuringiensis

37

Insecticidal toxin ; Production of Biological Insecticides; Bioassay of Biological Insecticides; Formulation and Use of Bioinsecticides; Safety Testing of Bioinsecticides; Search and Development of New Bioinsecticides.

Section - II3 Primary metabolites production 15

3.1 Amino Acids: Introduction, Microbial strain employed in aminoacid production, process control in amino acid fermentation, Production of Glutamic Acid by Wild Type Bacteria, Production of Amino Acids by Mutants, Improvements in the Production of Amino Acids Using Metabolically Engineered Organisms; Vitamin: Vitamin B12, Riboflavin, Carotenodis

3.2 Production of Organic Acids: Citric, acetic lactic, Gluconic and Itaconic acid

3.3 Production of Microbial enzyme: Introduction, Development of new enzymes, Fermentation process, Recovery and finishing, Regulations and specification, Survey of enzyme and application

3.4 Production of Fermented Foods: Introduction; Fermented Food from Wheat: Bread; Fermented Foods Made from Milk; Fermented Foods from Corn; Fermented Vegetables; Fermentations for the Production of the Stimulant Beverages: Tea, Coffee, and Cocoa; Fermented Foods Derived from Legumes and Oil Seeds; Production of Beer, Wines and Spirits

4 Production of Secondary metabolites and other 154.1 Microbial Polysaccharides and Polyesters : Polysaccharides,

Xanthan Gum, Polyesters 4.2 Production of Ergot Alkaloids: Nature of Ergot Alkaloids, Uses of

Ergot Alkaloids and their Derivates, Production of Ergot Alkaloids, Physiology of Alkaloid Production

4.3 Microbial Production of Nucleocide and Nucelotides: Introduction, Methods for production

4.4 Single Cell Protein (SCP): Substrates for Single Cell Protein Production; Microorganisms Used in SCP Production; Use of Autotrophic Microorganisms in SCP Production; Safety of Single Cell Protein; Nutritional Value of Single Cell Protein; Yeast Production: Production of Baker s Yeast; Food Yeasts; Feed Yeasts; Alcohol ‟Yeasts; Alcohol Yeasts

List of experiment 1 Production of Amino acid (Glutamic acid)2 Production of Vitamins (Vitamin B2 /Vitamin B12)3 Production of Solvents (Ethanol, Acetone/ Butanol)4 Production of Organic acid (Citric acid/ Gluconic acid) 5 Production of extra-cellular polysaccharide 6 Production of Antibiotics (Penicillin/ Tetracycline/ Chlormphanicol/ Streptomycin)7 Effectiveness of antimicrobial preservatives8 Microbial limit test

38

9 Physicochemical test of extracts: Preparation of extract, appearance, light absorption, pH, nonvolatile matter, residues on ignition, heavy metal, buffering capacity, oxidizable substances

10 Lal test for bacterial endotoxins

Reference Book :-No Book Name 1 Fermentation microbiology and Biotechnology, Taylor and Francis Mansi2 Industrial Microbiology: An Introduction, Blackwell publication Waites3 Bioprocess Engineering Basic Concept, Prentica Hall of India Michal4 Operational Models for Bioreactor, Biotol series5 Product recovery in Bioprocess technology, Biotol series6 Principles of fermentation technology. Whittaker7 A text book of Industrial microbiology. Crueger8 Modern Industrial Microbiology and biotechnology Okafor9 Applied Bioinformatics Najafpour10 Microbial Biotechnology Alexander11 Microbial Fundamentals of Biotechnology Volkmar12 Techniques in Applied Microbiology Sikyta13 Applied Microbiology, Volume 56 Laskin14 Advances in Applied Microbiology Volume 59 Laskin15 Advances in Applied Microbiology Volume 62 Laskin16 Microbial Processes and Products Walker17 Manual of Industrial and Biotechnology Demain18 Microbial technology: fermentation technology Peppler19 Industrial Microbiology: An Introduction Waites20 Bioprocess Engineering Basic Concept Michal21 A multivolume Comprehensive Treaties: Biotechnology Rehm

39


OMICS, INTER PHASE MICROBIOLOGY AND RECENT ADVANCES IN MICROBIOLOGY (RAM)Course type : Core Compulsory Total Credit : 04


Theory (hrs)

Practical (hrs)

Internal(Marks)

External(Marks)

Total(Marks)

4 X 15 = 60 30 70 ( Paper of 3 hrs) 100

Unit No.


sMarks Credit

Section - II1 Proteomics: Microbiology 15

1.1 Holistic Biology of Microorganisms: Genomics, Transcriptomics and Proteomics; Understanding genes, genomes, “otheromes”; Introduction and basic concept of systems biology.

1.2 Tools and technique for proteomics 1.3 Exploring and Exploiting Bacterial Proteomes; Strategies for

Measuring Dynamics: The Temporal Component of Proteomics; Quest for Complete Proteome Coverage

1.4 Proteomics of Corynebacteriumglutamicum: Essential Industrial Bacterium; Analyzing Bacterial Pathogenesis at Level of Proteome; Structural Proteomics and Computational Analysis of a Deadly Pathogen: Combating Mycobacterium tuberculosis from Multiple Fronts

2 Genomics: Microbiology2.1 Tools and technique for Genomics 152.2 Bacterial Genomes for the Masses2.3 Comparative Genomics for Microorganisms; Microbial Genome

Sequencing and Annotation2.4 Pharmacogenomics : Overview, concept and application of

Individualized Therapy; RNA Interference: Targeted Medicine

Section - II3 Metagenomics 15

3.1 Metagenomics: What and Why for metagenomics3.2 Metagenomics: a new light on biology 3.3 Metagenomics: From Genomics to Metagenomics3.4 Designing a successful metagenomics project

4 Recent Advances in Microbiology 154.1 Overview of clinical laboratory diagnosis (Hematology, Cardiac,

Renal, Liver testing’s) ; Culturing of pathogens; Immunological Diagnostic Procedure; Monoclonal Antibodies; DNA diagnosis systems; Molecular Diagnosis of genetic disease.

4.2 Overview and Current status of Anti HIV, Anti Malaria, Anti Tuberculosis and Anti Cancer treatment; Multidrug resistance : Introduction, development, detection and treatment

4.3 Discovering New Pathogens; New disease: SARS, bird flu , swine flu

40

etc.; Pharmacogenomics : Overview, concept and application of Individualized Therapy; RNA Interference: Targeted Medicine; Introduction to synthetic biology; Overview of Artificial Cells

4.4 Study of Selected recent review/ research paper in field of Microbiology ( Minimum four)

List of experiment 1 DNA extraction from Soil2 DNA extraction from water3 Library creation from metagenome4 Isolation of m RNA5 RT PCR6 Immunological testing : Widal test; VDRL test; Enzyme Linked Immuno Sorbent assay (ELISA)7 Hematology : RBC Count; Total WBC Count; Differential WBC Count; E.S.R. determination; Hb

estimation; 8 Bleeding time and clotting time9 Blood Grouping: Slide technique; Tube technique; Reverse and forward grouping/ Cross

matching: Major and Minor/Coombs test: Direct coomb’s; Indirect coomb’s10 Isolation and identification of Pathogens11 Biochemistry : Cardiac Profile testing; Live Profile testing; Renal Profile testing 12 Study of genome database and tools13 Study of Metagenomics database and tools14 Study of proteomics database and tools

Reference Book :-No Book Name 1 Genomics, Proteomics and clinical bacteriology Woodford2 Computing for Comparative Microbial Genomics Andreas3 Microbial Proteomics Humphery-Smith4 Protein Biochemistry and Proteomics Rehm5 Introduction to Proteomics Daniel6 Industrial Pharmaceutical Biotechnology Heinrich7 Bio safety in Microbiological and Biomedical Laboratories Richmond8 Drugs: From Discovery to Approval Rick9 Handbook of Pharmaceutical Biotechnology Gad10 Biopharmaceuticals Biochemistry and Biotechnology Walsh11 Pharmaceutical Microbiology, Blackwell scientific Publications Hugo12 Molecular Biotechnol Glick


ENZYME TECHNOLOGY (ETL)Course type : Elective Subject Total Credit : 02

41


Theory (hrs)

Practical (hrs)

Internal(Marks)

External(Marks)

Total(Marks)

4 X 15 = 60 50 ( Paper of 3 hrs) 50


DRUG DISCOVERY AND CLINICAL RESEARCH (DCR)Course type : Elective Subject Total Credit : 02


Theory (hrs)

Practical (hrs)

Internal(Marks)

External(Marks)

Total(Marks)

4 X 15 = 60 50 ( Paper of 3 hrs) 50


MICROBIAL PRODUCTION OF RECOMBINANT MOLECULES (MPR)Course type : Elective Subject Total Credit : 02


Theory (hrs)

Practical (hrs)

Internal(Marks)

External(Marks)

Total(Marks)

4 X 15 = 60 50 ( Paper of 3 hrs) 50

HEMCHANDRACHARYA NORTH GUJARAT UNIVERSITY, PATAN


MICROBIAL ECOLOGY (MEL)Course type : Elective Subject Total Credit : 02

42


Theory (hrs)

Practical (hrs)

Internal(Marks)

External(Marks)

Total(Marks)

4 X 15 = 60 50 ( Paper of 3 hrs) 50


CONTEMPORARY MICROBIOLOGY (CMB)Course type : Elective Subject Total Credit : 02


Theory (hrs)

Practical (hrs)

Internal(Marks)

External(Marks)

Total(Marks)

4 X 15 = 60 50 ( Paper of 3 hrs) 50


SOFT SKILL (SSK)Course type : Elective Subject Total Credit : 02


Theory (hrs)

Practical (hrs)

Internal(Marks)

External(Marks)

Total(Marks)

4 X 15 = 60 50 ( Paper of 3 hrs) 50

43

HEMCHANDRACHARYA NORTH GUJARAT UNIVERSITY, PATANProgramme code : MSMIC Programme Name : M.Sc. MICROBIOLOGY Course Code MSMIC101PR Semester : 1

PRACTICAL – III (PRACTICAL BASED ON MB 101 TO 103 AND SELECTED ELECTIVE PAPER) (LB1)



Theory (hrs)

Practical (hrs)

Internal(Marks)

External(Marks)

Total(Marks)

4 X 15 = 60 150 ( Paper of 3 hrs) 150

44

HEMCHANDRACHARYA NORTH GUJARAT UNIVERSITY, PATANProgramme code : MSMIC Programme Name : M.Sc. MICROBIOLOGY Course Code MSMIC401MP Semester : 4

Project Work (PRW)Course type : Minor project Total Credit : 02


Theory (hrs)

Practical (hrs)

Internal(Marks)

External(Marks)

Total(Marks)

4 X 15 = 60 50 ( 2 hrs ) 50

Unit No.


sMarks Credit

1 Project Work (Any paper of students’ choice to develop technical skill)

2

Students have to take up a small research project under the supervision of a teacher from the department or may carry the work in an industry / NGO / private laboratory with required facility/other university or institution. If a student is doing project out side the university then the chemicals to carry out experiment shall be replaced to the institute where he/she carry out the experimental work. All other arrangements are to be made by the student. Student has to submit the dissertation before the last date for the submission as declared by the university.

Documents

Web viewThe Nature of Metabolic Pathways; Industrial Microbiological Products as Primary and Secondary Metabolites; Trophophase-idiophase Relationships in the Production of Secondary