Computer 24B 24B - 2 Biology with Vernier 1 Figure 1 MATERIALS computer 600 mL beaker (for water bath) Vernier computer interface dropper or Beral pipet Logger Pro cooking oil (canola, olive, or corn) Vernier Gas Pressure Sensor three 18 150 mm test tubes 1-hole rubber stopper and tubing assembly hot and cold water or a water bath lactase solution stopwatch 5% galactose solution Tes-Tape or other glucose test paper 5% glucose solution test-tube rack 5% lactose solution thermometer 10 mL graduated cylinder yeast suspension PROCEDURE Testing for the Production of Glucose 1. You will determine if lactase produces glucose during the conversion of lactose. 2. Obtain two test tubes and label them test tube 1 and test tube 2. Table 1 Test tube Lactose sugar solution Lactase 1 2.5 mL 2 drops 2 2.5 mL none 3. Obtain the lactose sugar solution. Add 2.5 mL of the sugar solution to both test tubes, as listed in Table 1. Note: Do not add the lactase to the test tube until Step 7. 4. Prepare a water bath for the sugar solutions. The water level in the tank should cover 3/4 or more of the test tube while maintaining a temperature between 35 o C and 37 o C. If a water bath is not available, combine some warm and cool water in the 600 mL beaker to establish and maintain a 35 o C37 o C bath. If you need to add more hot or cold water to maintain a constant temperature, first remove about as much water as you will be adding, or the beaker may overflow. Use a basting bulb or a Beral pipet to remove excess water.
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