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第七章 白细胞分化抗原和黏附分子
Leukocyte Differentiation Antigen
第一节 白 细 胞 分 化 抗 原
Leukocyte Differentiation Antigen
Cell surface molecules expressed (or disappeared) during different developmental and differential phases, activation or inactivation process of blood cells.
LDAs on different types of cells
Endothelial cell
Tumor cells Glial cell
Neuron
Erythrocyte
Monocyte
Eosinophil
Platelets
Lymphocyte
Neutrophil Basophil
LDA in T cell development
Fayard E. Sci. Signal. 2010
Cluster of Differentiation
The same antigen recognized by different monoclonal antibody from different labs are called CD.
Cell
AntigenX
A B C D E
CD1xx
ImmunophenotypingType of cell CD markers
stem cells CD34+, CD31-
all leukocyte groups CD45+
GranulocyteCD45+, CD15+, CD24+, CD114+, CD182+
MonocyteCD45+, CD14+, CD114+, CD11a, CD91+
T lymphocyte CD45+, CD3+
T helper cell CD45+, CD3+, CD4+
T regulatory cell CD4, CD25, and Foxp3
Cytotoxic T cell CD45+, CD3+, CD8+
B lymphocyteCD45+, CD19+ or CD45+, CD20+, CD24+
Thrombocyte CD45+, CD61+
Natural killer cell CD16+, CD56+, CD3-, CD31, CD30
Adhesion Molecules 第二节 粘附分子
Cell Movement-Chasing
Chemokine gradient
Cell Movement-Basics
Definition--Adhesion Molecule
Cell adhesion molecules (CAMs) are cell surface proteins involved in the interaction of cell-cell or cell-extracellular matrix.
CAMs take effect by the binding of receptor and ligand.
Classification
Integrin familySelectin family Immunoglobulin (Ig) superfamilyCadherin family Mucin-like familyOther adhesion molecules
I. Integrin Family
Consist of α and β chains.
According to β subunits, Integrins are divided into eight groups: β1- β8
b1 Integrin Family
Interaction with ECM
b2 Integrin Family
Integrins promote immune response
II Selectin family
Selectins consist of one peptide chain.
The three family members include: E-selectin, L-selectin, and P-selectin.
Structure
Function
Cell Recruitment Cascade
III Immunoglobulin superfamily(IgSF)
The structure of these adhesion molecules resemble that of Ig;
CD4, CD8, CD2(LFA-2), TCR, MHC I, II molecules;
IgSF family and their ligands
粘附分子的功能
Cell
movement;
Cell activation.
I. CDs in T cell activation
LFA-1ICAM-1
II. CDs in cell movement
CDs and AMs in Medicine第三节临床医学中的分化抗原和粘附分子
I. Anti-CD3 Ab in transplantation
T cell activation in transplantation
Clinical use of humanized CD3-specific antibodies
II. Anti-CD20 and non-Hodgkin’s lymphoma
CD20 expression in B-cell development
III. CD4, co-receptor for HIV
AIDS
The replicative cycle of HIV
CD4/CD8 ratio
I. Targeting Adhesion molecules on tumor cells
Metastatic Process
Dissociation of tumor cells
Intravasation
Survival in circulation
Tumor cell proliferation at metastatic site
Extravasation
Yoon KJ Can. Biol. Ther 2009
Cell adhesion molecules involved in breast cancer metastasis
Drugs target CAMs
II Blocking a4b1 integrin as therapy for MS
Pathophysiology of MS
α4β1 integrin in cell adhesion
Steinmen L Nat. Rev. Drugdisc 2005
Blockade of α4β1 integrin
Key points in the development of Natalizumab
本章重点
名词解释: CD ;
粘附分子的定义;
粘附分子和分化抗原的功能。