© 2013 Pearson Education, Inc. Chapter 3: Observing Microorganisms Through a Microscope $100 $200 $300 $400 $500 $100$100$100 $200 $300 $400 $500 Compound

© 2013 Pearson Education, Inc.

Chapter 3: Observing Microorganisms Through a Microscope

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Compound Microscope

MicroscopyElectron

Microscopy

Preparing Smears for

StainingStaining

FINAL ROUND


What is the function of the diaphragm on a compound microscope?

a. magnifies the specimen

b. focuses light through the specimen

c. holds the microscope slide in position

d. controls the amount of light entering the condenser

Topic 1: Compound Microscope

$100 Question

ANSWER

BACK TO GAME



$100 Answer

What is the function of the diaphragm on a compound microscope?

a. magnifies the specimen

b. focuses light through the specimen

c. holds the microscope slide in position

d. controls the amount of light entering the condenser

BACK TO GAME



$200 Question

The ocular lens is used to

a. magnify the image from the objective lens.

b. improve resolution.

c. increase the light.

d. decrease the refractive index.

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ANSWER



$200 Answer

The ocular lens is used to

a. magnify the image from the objective lens.

b. improve resolution.

c. increase the light.

d. decrease the refractive index.

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$300 Question

Brightfield illumination is produced by the

a. condenser.

b. objective.

c. lens.

d. annular diaphragm.

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ANSWER



$300 Answer

Brightfield illumination is produced by the

a. condenser.

b. objective.

c. lens.

d. annular diaphragm.

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$400 Question

Most ocular lenses magnify specimens by a factor of

a. 100.

b. 10.

c. 1000.

d. 2000.

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ANSWER



$400 Answer

Most ocular lenses magnify specimens by a factor of

a. 100.

b. 10.

c. 1000.

d. 2000.

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$500 Question

The ability of the lenses to distinguish fine detail and structure is called

a. illumination.

b. magnification.

c. refractive index.

d. resolution.

BACK TO GAME

ANSWER



$500 Answer

The ability of the lenses to distinguish fine detail and structure is called

a. illumination.

b. magnification.

c. refractive index.

d. resolution.

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Which microscope would you choose to view the cellular contents of a microbe in its natural state?

a. compound light microscope

b. phase-contrast microscope

c. darkfield microscope

d. electron microscope

Topic 2: Microscopy

$100 Question

BACK TO GAME

ANSWER


Topic 2: Microscopy

$100 Answer

Which microscope would you choose to view the cellular contents of a microbe in its natural state?





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Which microscope uses an ultraviolet light source?



c. fluorescence microscope


Topic 2: Microscopy

$200 Question

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ANSWER


Which microscope uses an ultraviolet light source?



c. fluorescence microscope


Topic 2: Microscopy

$200 Answer

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Which microscope achieves the highest magnification and the greatest resolution?





Topic 2: Microscopy

$300 Question

BACK TO GAME

ANSWER


Which microscope achieves the highest magnification and the greatest resolution?





Topic 2: Microscopy

$300 Answer

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Topic 2: Microscopy

$400 Question

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ANSWER

Which microscope is especially useful in studying the surface structures of intact cells and viruses?

a. transmission electron microscope

b. scanning electron microscope

c. phase-contrast microscope

d. darkfield microscope


Topic 2: Microscopy

$400 Answer

BACK TO GAME

Which microscope is especially useful in studying the surface structures of intact cells and viruses?

a. transmission electron microscope

b. scanning electron microscope


d. darkfield microscope


Topic 2: Microscopy

$500 Question

What microscope should be used to view three-dimensional images?

a. differential interference contrast microscope

b. darkfield microscope


d. transmission electron microscope

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ANSWER


Topic 2: Microscopy

$500 Answer

What microscope should be used to view three-dimensional images?

a. differential interference contrast microscope

b. darkfield microscope


d. transmission electron microscope

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Topic 3: Electron Microscopy

$100 Question

Objects smaller than about 0.2 micrometers, such as viruses, must be examined with a(n)

a. compound microscope.

b. electron microscope.

c. phase-contrast microscope.

d. simple microscope.

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ANSWER



$100 Answer

Objects smaller than about 0.2 micrometers, such as viruses, must be examined with a(n)

a. compound microscope.

b. electron microscope.

c. phase-contrast microscope.

d. simple microscope.

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$200 Question

In electron microscopy, the specimen is placed on a

a. copper mesh grid.

b. glass slide.

c. plastic slide.

d. magnesium mesh grid.

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ANSWER



$200 Answer

In electron microscopy, the specimen is placed on a

a. copper mesh grid.

b. glass slide.

c. plastic slide.

d. magnesium mesh grid.

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Shadow casting is useful

a. for the study of the very smallest specimens, such as virus particles.

b. to provide a general idea of size and shape of the specimen.

c. to increase the electron opacity of the surrounding field.

d. to enhance the contrast between the specimen and the background.


$300 Question

BACK TO GAME

ANSWER


Shadow casting is useful


$300 Answer

a. for the study of the very smallest specimens, such as virus particles.

b. to provide a general idea of size and shape of the specimen.

c. to increase the electron opacity of the surrounding field.

d. to enhance the contrast between the specimen and the background.

BACK TO GAME



$400 Question

In a scanning electron microscope, objects are generally magnified

a. 100 to 100,000x.

b. 100 to 10,000x.

c. 1000 to 100,000x.

d. 1000 to 10,000x.

BACK TO GAME

ANSWER



$400 Answer

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In a scanning electron microscope, objects are generally magnified

a. 100 to 100,000x.

b. 100 to 10,000x.

c. 1000 to 100,000x.

d. 1000 to 10,000x.



$500 Question

In a transmission electron microscope, objects are generally magnified

a. 1000 to 100,000x.

b. 1000 to 10,000x.

c. 10,000 to 100,000x.

d. 10,000 to 1,000,000x.

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ANSWER



$500 Answer

In a transmission electron microscope, objects are generally magnified

a. 1000 to 100,000x.

b. 1000 to 10,000x.

c. 10,000 to 100,000x.

d. 10,000 to 1,000,000x.

BACK TO GAME


Why are smear preparations fixed?

a. to kill and color the microorganisms

b. to color the microorganisms and fix them to the slide

c. to kill the microorganisms and fix them to the slide

d. to kill and color the microorganisms and fix them to the slide

Topic 4: Preparing Smears for Staining

$100 Question

BACK TO GAME

ANSWER


Why are smear preparations fixed?


$100 Answer

a. to kill and color the microorganisms

b. to color the microorganisms and fix them to the slide

c. to kill the microorganisms and fix them to the slide

d. to kill and color the microorganisms and fix them to the slide

BACK TO GAME



$200 Question

Basic dyes are _________ ions and are _________ to most types of bacteria.

a. positive; attracted

b. negative; attracted

c. positive; not attracted

d. negative; not attracted

ANSWER

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$200 Answer

Basic dyes are _________ ions and are _________ to most types of bacteria.

a. positive; attracted

b. negative; attracted

c. positive; not attracted

d. negative; not attracted

BACK TO GAME


a. observing shapes, sizes, and capsules of microorganisms

b. killing the microorganisms and fixing them to the slide

c. spreading a thin film of material containing microorganisms on a slide

d. coloring the microorganisms with a dye that emphasizes certain structures

What is staining?


$300 Question

BACK TO GAME

ANSWER


What is staining?


$300 Answer

a. observing shapes, sizes, and capsules of microorganisms

b. killing the microorganisms and fixing them to the slide

c. spreading a thin film of material containing microorganisms on a slide

d. coloring the microorganisms with a dye that emphasizes certain structures

BACK TO GAME



$400 Question

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ANSWER

To stain an organism correctly, one must first

a. kill the organism.

b. stain the slide.

c. prepare a smear.

d. fix the slide.



$400 Answer

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To stain an organism correctly, one must first

a. kill the organism.

b. stain the slide.

c. prepare a smear.

d. fix the slide.



$500 Question

Distortions of cell size and shape are minimized in

a. negative staining.

b. Gram staining.

c. acid-fast staining.

d. endospore staining.

BACK TO GAME

ANSWER



$500 Answer

Distortions of cell size and shape are minimized in

a. negative staining.

b. Gram staining.

c. acid-fast staining.

d. endospore staining.

BACK TO GAME


Topic 5: Staining

$100 Question

What is the mordant in the Gram stain?

a. iodine

b. safranin

c. alcohol-acetone

d. crystal violet

BACK TO GAME

ANSWER


Topic 5: Staining

$100 Answer

What is the mordant in the Gram stain?

a. iodine

b. safranin

c. alcohol-acetone

d. crystal violet

BACK TO GAME


Topic 5: Staining

$200 Question

Flagella are stained with a(n)

a. carbolfuchsin stain.

b. malachite green stain.

c. methylene blue stain.

d. acid-fast stain.

BACK TO GAME

ANSWER


Topic 5: Staining

$200 Answer

Flagella are stained with a(n)

a. carbolfuchsin stain.

b. malachite green stain.

c. methylene blue stain.

d. acid-fast stain.

BACK TO GAME


Topic 5: Staining

$300 Question

Capsules are stained with a(n)

a. negative stain.

b. Gram stain.

c. Schaeffer-Fulton stain.

d. acid-fast stain.

BACK TO GAME

ANSWER


Topic 5: Staining

$300 Answer

BACK TO GAME

Capsules are stained with a(n)

a. negative stain.

b. Gram stain.


d. acid-fast stain.


Topic 5: Staining

$400 Question

Endospores are stained with a(n)

a. negative stain.

b. Gram stain.


d. acid-fast stain.

BACK TO GAME

ANSWER


Topic 5: Staining

$400 Answer

Endospores are stained with a(n)

a. negative stain.

b. Gram stain.


d. acid-fast stain.

BACK TO GAME


Topic 5: Staining

$500 Question

What color are gram-negative bacteria at the completion of the Gram staining process?

a. pink

b. purple

c. colorless

d. brown

BACK TO GAME

ANSWER


Topic 5: Staining

$500 Answer

What color are gram-negative bacteria at the completion of the Gram staining process?

a. pink

b. purple

c. colorless

d. brown

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FINAL ROUND Question

The appearance of Mycobacterium leprae after they are stained with an acid-fast stain is

a. purple.

b. red.

c. colorless.

d. brown.

BACK TO GAME

ANSWER


FINAL ROUND Answer

The appearance of Mycobacterium leprae after they are stained with an acid-fast stain is

a. purple.

b. red.

c. colorless.

d. brown.

BACK TO GAME

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© 2013 Pearson Education, Inc. Chapter 3: Observing Microorganisms Through a Microscope $100 $200 $300 $400 $500 $100$100$100 $200 $300 $400 $500 Compound