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© 2007, YCMOU. All Rights Reserved. 1
Online Counseling ResourceYCMOU ELearning Drive…
School of Architecture, Science and TechnologyYashwantrao Chavan Maharashtra
Open University, Nashik – 422222, India
© 2007, YCMOU. All Rights Reserved. 2
OC-SBT081-CP01-01
Introduction
Programmes and Courses SEP – SBT081 – Unit 01
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Credits
Academic Inputs by Arun Punaji More.
M.Sc. (Microbiology) Experience: 11 Years [email protected]
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How to Use This Resource
Counselor at each study center should use this presentation to deliver
lecture of 40-60 minutes during Face-To-Face counseling.
Discussion about students difficulties or tutorial with assignments should
follow the lecture for about 40-60 minutes.
Handouts (with 6 slides on each A4 size page) of this presentation should
be provided to each student.
Each student should discuss on the discussion forum all the terms which
could not be understood. This will improve his writing skills and enhance
knowledge level about topics, which shall be immensely useful for end
exam.
Appear several times, for all the Self-Tests, available for this course.
Student can use handouts for last minutes preparation just before end
exam.
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Learning Objectives
After studying this module, you should be able to: Describe Morphological features of bacteria. State examples of bacteria showing different
types of morphology. Classify bacteria depending upon their
morphology.
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Introduction Bacteria exhibit variety of morphological
forms. The morphological forms of bacteria can
be used in identification of the bacterial species.
Identification of bacteria is essential to classify bacteria, to diagnose a pathogenic bacteria in a particular disease for proper treatment of the disease.
It is therefore interesting to know the different morphological features of bacteria.
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Morphology of Bacteria-1 Bacteria exhibit various shapes and range from
1μ to 10μ. The bacteria show three basic shapes such as Rod shaped bacteria or bacilli, spherical or cocci shaped bacteria and Spiral shaped bacteria. Bacteria show some slight variations in these
shapes. For examples rod shaped bacteria show slight
curved ends giving them comma shaped appearance; this is called as comma shaped bacteria.
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Morphology of Bacteria-2 The typical morphological features of
bacteria are shown in the following figure.
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Morphology of Bacteria-3 Vibrio cholerae is example of comma
shaped bacteria. Some bacteria show shapes
intermediate between spherical and rods; these are called as cocco-bacilli.
Spirilla and spirochetes are examples of spiral bacteria.
Besides these shapes, bacterial morphology is also described with their some special appendages such as pilli, flagella, and resistant forms, spores.
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Morphology of Bacteria-4 The motility of bacteria can be seen
by hanging drop technique and is used to identify and classify bacteria into motile and non motile bacteria.
Flagellum is organ of motility and give bacteria capability to move toward nutrient riched environment for their survival
Fig: Bacterial cell with polar flagella
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Morphology of Bacteria-5 Morphology of bacteria can be observed
after staining them with Gram's stains under compound microscope.
Here the bacilli are arranged in chains and endospores can also be seen.
Fig: Bacilli stained with Gram's stain.
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Morphology of Bacteria-6 Spherical or cocci shaped bacteria:-
The bacteria which show spherical morphology are called as cocci.
Cocci show different cell arrangements which help further to classify them into two genus i. e. staphylococci and streptococci.
The cocci which appear in bunches are called as Saphylococci.
The cocci which appear in chains are called as Streptococci.
Streptococci and Staphylococci are Gram positive bacteria.
Many species of streptococci possess capsule which is a major virulence factor of these bacteria.
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Morphology of Bacteria-7
Some bacteria show intermidiate morphology between rod and cocci; these are called as coccobacilli.
Enterobacter earogenes are coccobacilli; they one of the major causative organisms of nosocomial infections.
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Morphology of Bacteria-8 Gram staining of bacteria culture
differentiate the rod shaped bacteria from the spherical shaped bacteria.
Fig: Gram stained smear of rod shaped and spherical shaped bacteria.
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Morphology of Bacteria-9 Corynebacteria show pleomorphic
morphology. Following Figure shows Corynebacteria
diphtheria arranged in chines letter forms.
Fig: Corynebacteria diphtheriae
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Morphology of Bacteria-10 Though the majority of cocci are Gram
positive by nature there are few exception.
The species of Niesseria are Gram negative bacteria which appear in pairs; therefore called as Gram negative diplococci.
This typical morphology of Niesseria in urethral pus formed a primitive diagnosis of disease, gonorrhea.
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Morphology of Bacteria-11 The genus Niesseria include two
medically important pathogens i.e. N. gonococci and N. meningitidis.
The gonococci show typical kidney shaped or bean shaped morphology as shown below.
Fig: Bean shaped gonococci arranged as dipococci.
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Morphology of Bacteria-12 Spiral bacteria:- Spirilla are spiral stiff
bacteria.
Fig: spirilla
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Morphology of Bacteria-13 Spiral shaped bacteria:-
Spirochete is another example of spiral shaped bacteria.
Unlike the spirilla they are flexible. The spirochete has three genera:
Treponema Borrelia Leptospira
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Morphology of Bacteria-14 Coccobacilli:-
Bacilli of yersenia pestis are causative agents of bubonic plague. Their another characteristic feature is that they show bipolar staining giving themSafety pin like appearance as shown in the figure.
Fig : yerseneia pestis, coccobacilli
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Morphology of Bacteria-15 Bacilli of Mycobacteria appear as long
slender rods as shown below.
Fig: Mycobacteria tuberculosis
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What We Learn... Different morphological formed of
bacteria. How morphology of bacteria help to
classify bacteria into different groups. How the morphology of bacteria help in
the diagnosis of some diseases. How to study morphology of bacteria.
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Critical Thinking Questions Why bacteria are stained to study their
morphology?
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Hints For Critical Thinking Questions Bacterial cells are colorless, and having no
contrast with their surrounding.
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Study Tips Book
Title:Title: Microbiology Author:Author: Robert W Bauman Publication:Publication: Benjamin Cummings, San Francisco
Book Title:Title: Microbiology, An Introduction Author:Author: Tortora and et al. Publication:Publication: Pearson education, Singapore
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End of the Presentation
Thank You!