Enzyme

1. Enzyme Notes 2. Enzyme lab pre & post questions (2

stamps) 3. Catalase lab data collection and rough draft 4. Notes on Enzyme Activity

Table of Contents

1. What indicator is used to test for the disaccharide sucrose?

2. When sucrose undergoes hydrolysis, what are the products of the reaction?

3. What kind of molecule speeds up the hydrolysis of sucrose?

4. What indicator can be used to detect the presence of the monomers produced?

Pre Lab Quiz

1. What indicator is used to test for the disaccharide sucrose? NONE!

2. When sucrose undergoes hydrolysis, what are the products of the reaction?Glucose and Fructose (monosaccharides)

3. What kind of molecule speeds up the hydrolysis of sucrose? Enzymes

4. What indicator can be used to detect the presence of the monomers produced? Benedicts (with heat)

Pre Lab Quiz

2–4 Chemical Reactions and Enzymes

A. Chemical ReactionsB. Energy in Reactions

1. Energy Changes2. Activation Energy

C. EnzymesD. Enzyme Action

1. The Enzyme-Substrate Complex2. Regulation of Enzyme Activity

Section 2-4

Section Outline

Chemical Reactions

Anatomy of a Chemical Equation

+ +Reactant Reactant Product Product

“Reacts with”“and”, etc.

YIELDS(“To produce”)

“and”

Chemical ReactionsExample

+ +Sucrose Water Glucose Fructose

“Reacts with”“and”, etc.

YIELDS(“To

produce”)

“and”

Enzyme

Reaction pathwaywithout enzyme Activation energy

without enzyme

Activationenergywith enzymeReaction pathway

with enzyme

Reactants

Products

Section 2-4

Effect of Enzymes

Enzyme/Substrate Complex

ENZYMES ARE PROTEINS

More examples of enzymes and substratedENZYME SUBSTRATE

Lactase Lactose

Sucrase

Amylase

Bromelin Pectin

Starch

Sucrose

Make a Hypothesis Draw the experimental setup Label beakers (DO NOT WRITE ON WHITE PART) Label test tubes (DO NOT WRITE ON WHITE

PART) Label pipettes Come to the front to get your chemicals After adding the Benedicts to the test tubes,

finger vortex for 30 seconds before putting in water bath.

Lab Advice

Return any unused solutions to the stock bottles up front

Rinse all test tubes and place them upside down to dry

Return evertyhing to the white basket.

Clean Up Instructions

You cut yourself – mom pours hydrogen peroxide on the cut.

What happens?Why?

Equation:

CATALASE AND H2O2

It Bubbles

There is a chemical reaction (and an enzyme)

+H2O2 H2O (l) O2 (g)

Catalase

You will be using a jumbo test tube instead of a cup

Pour your peroxide in s l o w l y! Otherwise it will overflow and you will not get a reading.

DO NOT POUR YOUR REACTION WASTE DOWN THE SINK. It is raw chicken liver and it will smell bad for months. There is a waste container up front for disposal.

Catalase Lab Advice

Average your times for each 30 second interval Get averages of other groups that had the

same amount of catalase as you. Average all those times for each 30 second

interval together. Make a rough draft graph of your now much

more accurate data Divide your graph into 2 sections (where the

graph slope changes drastically) HW) Find the rate of the first section in units of

degrees Celcius / second

Data and Graph rough draft

4a. Do your increase in rate calculations support your hypothesis concerning enzyme concentration? Explain how you calculated the rate.

4b. Why did the temperature decrease/plateau after reaching a peak?

Analysis Question Number 4

Enzyme Activity depends on factors such as

◦Temperature◦pH

Enzyme Activity

Explain this graphEnzyme works fastest

Enzyme Denatures (breaks down)

pH Scale and Enzymes

Explain this graph

What is the effect of increased catalase concentration on the breakdown of peroxide measured by increasing temperature of reaction?

Question for catalase lab

Energy-Absorbing Reaction Energy-Releasing Reaction

Products

Products

Activation energy

Activation energy

Reactants

Reactants

Section 2-4

Figure 2-19 Chemical Reactions

Energy-Absorbing Reaction Energy-Releasing Reaction

Products

Products

Activation energy

Activation energy

Reactants

Reactants

Section 2-4

Figure 2-19 Chemical Reactions