Production of insulin from microbial culture

PRODUCTION OF INSULIN FROM MICROBIAL CULTURE

RUI BAIMUHAMMAD AKLIL BIN AYOB

YANGJIE CUI

CHEN 60351 : CORE BIOTECHNOLOGY 1

CONTENT1. Abstract2. Introduction3. Main process of insulin production4. Significance of human insulin5. Conclusion

Abstract Hormone secreted by pancreas Control blood sugar level Lack of insulin caused diabetes

mellitus Industrial production by

manipulating genetic engineering and microbial culture technology

Introduction Protein generated

by beta cell of pancreas (islet of Langerhans)

Exist in animal: porcine and bovine

Build up of 51 amino acids

30 amino acids form chain B

21 amino acids form chain A

2 chain linked by disulfide bonds

(Introduction)…cont

www.bio.davidson.edu

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(Introduction)…cont Diabetes mellitus

(DM) lack of insulin to control blood sugar level

Need to insulin therapy to keep healthy

www.som.uq.edu.au

Main process of insulin production Microorganisms

Fungi Yeast

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Microorganisms

Kinds of bacteria E. coli

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Genetic Engineering

http://blogs.swa-jkt.com/swa/10822/

Microbial Fermentation

Significance of human insulin 1978: insulin from animal; pig and cow Switch to microbial culture. Why?

Quantity & quality is better No allergy and side effect

E. coli culture is convenient Cheaper for commercial

Predictable Problems easily solved

Conclusion Insulin market grown at

7% annual rate Company: Novo

Nordisk, Eli Lilly & Sanofi Aventis

Cost : £75 – 200 per vial Different delivery ways

www.novonordisk.com

http://www.bd.com

Thank you!