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INTERLEUKIN-6 PLAYS A PROTECTIVE ROLE IN DEVELOPMENT OF CISPLATIN-INDUCED ACUTE RENAL
FAILURE THROUGH UPREGULATION OF ANTI-OXIDATIVE STRESS FACTORS
Daniel Saldarriaga
Melissa Zapata
III SEMESTER –MEDICINE
UPB
Satoru Mitazaki, Shigeyoshi Honma, Minwako Suto, Naho Kato, Kouichi Haraiwa, Makoto Yoshida, Sumiko Abe
INTRODUCTION
INTRODUCTION
Function is to regulate events such as activation, differentiation
or proliferation, antibody secretion, chemotaxis, regulation of cytokines
and other factors.
Synthesized primarily by leukocytes or endothelial cells, stromal thymus or
bone marrow.
Set of proteins that act as chemical
messengers within walking distance.
INTERLEUKIN
INTRODUCTION
Is a glycoprotein
Secreted by macrophages, T cells, endothelial cells and fibroblasts
inflammatory and proinflammatory activity
Involved in the production of immunoglobulins
the differentiation of B cells, activated cytotoxic T lymphocytes, plasma cells, modulates hematopoiesis and fibrinógeno
IL-6 with IL1 act with proteins in the acute phase
INTERLEUKIN-6
INTRODUCTION
Abrupt and sustained deterioration
of glomerular filtration expressed
initially by inability to excrete nitrogenous
products and a tendency to oliguria
Has a high mortality - on average 50%
INTRODUCTION
Alterations of the glomerular filtration
Intrarenal vasoconstriction
Tubular lumen obstruction
Backscatter glomerular filtration
rate
CAUSES
INTRODUCTION
Oxidative stress is caused by an imbalance between production of reactive
oxygen and the ability of a biological system to
detoxify reactive intermediates or quickly repa
ir the damage resulting.
OXIDATIVE STRESS
Can cause toxic effects through the production of
peroxides and free radicals that damage all
components of the cell, including proteins, lipids
and DNA.
INTRODUCTION
INTRODUCTION
BAD HABITS ALIMENTICION
STRESSSMOKE SNUFF
RADIATIONALCOHOL
DEPRESSIONASIEDAD SEDENTARY
INTRODUCTION
Platinum-based drug used in
chemotherapy to treat various types of
cancer, which include sarcomas, some
carcinomas lymphomas and germ cell tumor
CISPLATIN
CORRELATION
OBJECTIVE
MAIN OBJETIVE
Asses the role of the interleukin-6 like a
protective factor in acute renal failure through some experiment, to check the
hypotesis
MATERIALES Y METODOS
MATERIALES Y METODOS
ANIMALES
ACCION DE LA IL – 6 KNOCKOUTSIMULACION DEL HUMANO
MATERIALES Y METODOS
WESTERN BLOTTING
DETECTA PROTEINAS ESPECIFICAS POR ELECTROFORESIS EN GEL.
MATERIALES Y METODOS
INMUNOHISTOQUIMICA
METODO QUE UTILIZA DE ANTICUERPOS ESPECIFICOS APLICADA A UN TEJIDO
MATERIALES Y METODOS
PCR TIEMPO REAL
Amplificar y cuantificar a la misma ves producto de la amplificación ADN
RESULTADOS
RESULTADOS
• Fig 1
RESULTADOS
• Fig 3
RESULTADOS
• Fig 5
RESULTADOS
• Fig 6
DISCUSSION
RESEARCHER ENDS YES NO
Mitazaki et al., 2009 IL-6 produced in renal tubular cells is thought to prevent progression of acute renal failure at the early stage.
Matsushima et al., 1998 lameire et al., 2005
IL-6 plays a protective role in the development of cisplatin- induced acute renal failure through upregulation of anti-oxidativ stress factors.
RESEARCHER ENDS YES
NO
Dixit et al., 2009 Increase of A-HNE modified prteins is commonly used as an indicator of lipid peroxidationt. Therefore, lack of IL-6 migh be responsible for the iincrease of lipid peroxidation.
James et al., 2005 Unregulated cox-2 expression and prostaglandin synthesis influence inflammation
CONCLUSIONS
CONCLUSIONS
Such activities promote student skills in developing experimental and innovative research to generate awareness investigative.
It is important to apply the findings to clinical practice, because they feed the knowledge learned in formal education.
CONCLUSIONS
The current development of medicine has been marked by many discoveries about the metabolic function of cells and the development of multiple types of analysis in biological samples, these are of great importance of medical diagnostic.
CONCLUSIONS
The importance of molecular biomarkers such as IL-6, the protective acute renal failure are useful elements for its association to diseases.
Daniel Saldarriaga Arango
Melissa Zapata Durán
THANKS!
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