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DISSERTATION ON DISSERTATION ON
The purpose of this present study was to study effect of various concentration of TDZ on the Diploid and Tetraploid cotton on multiple shoot induction. The objectives are. . .
•In-vitro germination of seeds•Isolation of different types of explants from different age group of seedlings. •Culture of different types of explants on various concentration of phytohormone.•Regeneration of plant by multiple shoots induction or callus induction
Macro Element Quantity (gm/L)
NH4 NO3
KNO3
CaCl2. H2O
MgSO4. H2O
KH2PO4
16.5
19.0
4.4
3.7
1.7
Micro Element Quantity (gm/L)
KI
H3BO4
MnSO4.4H2O
ZnSO4.7H2O
Na2MnO4.2H2O
CuSO4.5H2O
CoCl2. 6H2O
0.083
0.62
2.23
0.86
0.025
0.0025
0.0025
Lot No. No. Of Seeds
Inoculated
No. Of Seeds
Germinated
Germination (%)
1 94 67 71.27
2 89 69 77.52
3 120 83 69.16
4 92 72 78.26
5 79 51 64.55
6 130 98 75.38
7 87 71 81.60
8 140 103 73.57
9 77 54 70.12
10 116 84 72.41
TOTAL 1024 752 73.38 %
Media Combinati
on(Mg/L
Replication Explants Cultured Explants Responded
Proliferation %
Multiple Shoots Observed
11 DAYS 25 DAYS
0.02 TDZ
R I 8 7 2.57 2.85R II 9 9 1.77 2.77
Total 17 16 94.11% 2.17 2.810.05 TDZ
R I 9 8 4.25 5.87R II 9 9 3.55 5.33
Total 18 17 94.44% 3.90 5.660.08 TDZ
R I 7 7 3.85 7.57R II 9 9 2.66 5.77
Total 16 16 100% 3.25 6.670.1 TDZ R I 8 6 2.50 5.00
R II 8 8 1.50 3.16Total 16 14 87.50% 2.00 4.08
Media Combinati
on(Mg/L
Replication Explants Cultured Explants Responded
Proliferation %
Multiple Shoots Observed
11 DAYS 25 DAYS
0.02 TDZ
R I 7 7 4.14 6.42R II 9 9 3.00 4.44Total 16 16 100% 3.57 5.43
0.05 TDZ
R I 6 5.66 8.33R II 8 6 4.66 9.66Total 14 12 85.71% 5.16 8.99
0.08 TDZ
R I 6 6 4.33 9.33R II 8 8 3.75 5.00Total 14 14 100% 4.04 7.16
0.1 TDZ
R I 4 3 3.00 4.66R II 7 4 5.00 6.00Total 11 7 63.63% 4.00 5.33
Media Combinati
on(Mg/L
Replication Explants Cultured Explants Responded
Proliferation %
Multiple Shoots Observed
11 DAYS 25 DAYS
0.02 TDZ R I 8 5 4.50 7.00R II 6 6 3.80 5.40R III - -Total 20 17 85% 4.15 6.20
0.05 TDZ R I 7 5 3.25 5.00R II 6 5 3.40 7.40R III 6 4 - -Total 19 14 73.68% 3.32 6.20
0.08 TDZ R I 6 5 2.25 3.75R II 6 5 3.00 3.50R III 7 6 - -Total 19 16 84.21% 2.62 3.62
0.1 TDZ R I 7 4 2.66 4.33R II 6 6 2.80 5.00R III 6 3 - -Total 19 13 68.42% 2.73 4.66
Media
Combination
Replication Explants
Cultured
Explants
responded
Proliferation
Percentage
0.02 TDZ R I 9 9100%RII 12 12
Total 21 210.05 TDZ R I 8 6
76.47%RII 9 7Total 17 13
0.08 TDZ R I 10 348.14%R II 17 10
Total 27 130.1 TDZ R I 11 5
45%R II 9 4Total 20 9
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BISECT ½ VERTICAL SECTION OF SHOOT TIP
BISECT ¾ & ¼ SECTION OF SHOOT TIP
SHOOT TIP
SHOOT TIP
¼ SECTION ¾ SECTION
½ SECTION
THE END
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