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BrandeWulffJohnInnesCentreNorwich,UK
2
3
Edgar McFadden 1891-1956
“Itrequiresaboutthesamelengthof4metodevelopahybridstrainofwheatasitdoestoraisea[child]”
A.W.Erickson,1945
R1.X
.R1
Introducing resistance genes by wide crossing
R1.
Stacking cloned resistance genes
R2.
R3.R1R2R3
The three challenges of wild grasses
Agronomy
MutaGonalgenomics GenomecomplexityreducGonAlienintrogressions
.R1
Giantgenomes
Arabidopsis0.12GB
Wildgrass~7Gb
Wheat17Gb
GenesRgenes
SuppressedrecombinaGon
Recombina
Gon
Mutagenesis & sequencing
EMS
R1 R2 R3 R4 R5 R6 R7 R8 R9 R10 R11 R12 R13 R14 R2500Resistantparent ...
x x x xMutant1 ...
xx x x xxxxxx
xx
xx
xx
Mutant2
Mutant3
Mutant4
Mutant5
...
...
...
...
MutRenSeq:resistancegeneenrichmentsequencingofmutants
Baits
GenesRgenes
GenomicDNA
MutantHunter
1.Assemblewildtype
2.Mapmutants
3.DetectmutaGons
4.DeterminemutaGonoverlap
Candidategene
Wheatstemrust
1999
Wheatgrowingarea
2015 2008
2006
2013
IsolaGonofNB-LRRsbyMutRenSeq
1S 2S 3S1 5S1 6S 7S1Sr22Mutants
**
7,5005,0002,500
CC NB LRR5’UTR 3’UTR
RenSeqcon7g10555_1
RenSeqcon7g3841_1
bp0
FielderTransgenics
InancientRomanreligion,theRobigaliawasafes7valheldApril25.Itsmainritualwasadogsacrificetoprotectgrainfieldsfromdisease.Thesacrificialofferingwasthebloodandentrailsofanunweanedpuppy.
EnrichedDNATotalgDNA
2a Targetedenrichment
1 Mutagenesisandscreening
EMS
3 Bioinforma7csSequencing
R1 R2 R3 R4 R5 R6 R7 R8 R9 R10 R11 R12 R13 R14 R2500Resistantparent ...
x x x xMutant1
xx x x xxxxxx
xx
xx
xx
Mutant2
Mutant3
Mutant4
Mutant5 ..
...
...
...
...
1 2 3 4 5 6 7
12
345
6
7
Rela7veDNAcontentGAA-FITC
fluo
rescen
ce
2b Chromosomeflowsor7ngandamplifica7on
MutChromSeq
αβ-hydrolasefold5’UTR 3’UTR
ChromSeqcon7g_946_1
10 110 kb
2 **
Barleyeceriferum-q
ChromSeqcon7g_7526_1
010
**
NB-ARC LRRsCC
7
2
689
***
5’UTR 3’UTR
5kb
Wheatpowderymildewresistance2
Mutate RenSeq BioinformaGcs TransgenicScreenSr22
Sr45
Sr1644
Sr43
Sr44
Sr2020
Sr40
Sr25
Sr51
Sr53
Clone Stack Deploy
TradiGonalBreedingWildwheat
Breadwheat
Introgression
MutSeq
Clonedgene
ClonedgenesWildwheatdiversitypanel
AgRenSeq
Genepyramid
Engineering
OpenWildWheat
AliMehrabi,IlamUniversity,IranAlisonBentley,Na7onalIns7tuteofAgriculturalBotany,UKAmirSharon,Ins7tuteofCerealCropImprovement,IsraelBrandeWulff,JohnInnesCentre,UK BrianSteffenson,UniversityofMinnesota,USA BurkhardSteuernagel,JohnInnesCentre,UK CarolinaPaolaSansaloni,CIMMYT,MexicoEvansLagudah,CSIRO,AustraliaFiruzaNasyrova,AcademyofSciences,TajikistanHananSela,Ins7tuteofCerealCropImprovement,IsraelJanDvorak,UCDavis,USAJessePoland,KansasStateUniversity,USAKlausMayer,HelmholtzZentrumMünchen,GermanyKseniaKrasileva,EarlhamIns7tute,UKMarioCaccamo,Na7onalIns7tuteofAgricultualBotany,UKMarGnMascher,IPKGatersleben,GermanyMikeAmbrose,GermplasmResourcesUnit,UKMinghengLuo,UCDavis,USAParveenChhuneja,PunjabAgriculturalUniversity,India
AsyrafHacaBurkhardSteuernagelDeepikaNarangGuotaiYu LucyMcCannNgoniKangaraSanuAroraSreyaGhoshYajuanYue
Acknowledgements
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