Genetic engineering Course Overview

Course Overview

Mr. Veerendra Singh NagoriaAssistant Professor Biotechnology, School of Life Science, Rai University AhmedabadVeerendra.nagoria@gmail.com, veerendra@outlook.com

Minimum Attendance requirement to sit for final semester examination is 75%

No Assignment after defined time limit Open to discussion on Web over Google

group. PPTs can be taken from my Slideshare

account but it is limited in nature consult books

Participation during lecture session will be having additional weightage

CONTINUOUS EVALUATION 30 MarksMid-Term Assessment As per examination

Assignment 1 10 MarksViva on Assignment 1 5 Marks

Assignment 2 10 MarksViva on Assignment 2 5 Marks

Class Presentation 15 Marks

Class Participation 5 Marks

END SEMESTER EVALUATION 70 Marks

Introduction to concepts, Definitions, Discoveries

Overview of fundamental processes: Replication, Transcription, Translation

Basic Methodology Restriction modification system and

Restriction Enzymes DNA modification enzymes:

Nucleases, Polymerases, Enzymes modifying the ends of DNA molecule

Vectors: Plasmids, Bacteriophages, Cosmid, Phagemid, Artificial chromosomes

Host cell types- prokaryotic and eukaryotic

Transfer of vector into host cell: Transformation, Transfection, Packaging of phage DNA, Alternative DNA delivery methods

Cloning from mRNA, Genomic DNACloning after restriction digestion;

blunt and cohesiveCreation of cloning site by PCRCloning using Linkers and AdaptersCloning by recombination; GATEWAY

technologyTA Cloning, TOPO Cloning, Ligation

free cloning

Screening methods; Chromogenic substance, Insertional inactivation, Complementation of defined mutation

Selecting recombinant clones; Reporter gene, elimination of non-transformed cells, identification of clones having Recombinant DNA

Sequence Dependent screening; Colony hybridization, Chromosome walking, Screening by PCR, Gene Tagging

Genetic Engineering & Biotechnology; protein production, protein engineering, scale up

Medicinal & Forensic Application of Gene Manipulation; Diagnosis, Treatment and DNA profiling