Carbon dioxide mitigation by Nannochloropsis oculata towards biodiesel production

Name : Vijendren Krishnan

Chairman : Prof. Dr. Y. Uemura

(30 April 2014)

Research Progress

Carbon dioxide mitigation by

Nannochloropsis oculata

towards biodiesel production

Domain : Eukaryota

Kingdom : Chromalveolata

Phylum : Heterokontophyta

Class : Eustigmatophyceae

Family : Eustigmataceae

Genus : Nannochloropsis

Species : oculata

(Hibbert, 1981)

Taxonomy :

Nannochloropsis oculata (Hibbert)

• Small spheres with diameter of 2 – 4 µM

• One single Chloroplast without pyrenoid, butcontain several lamella, each with 3thylakoids.

• Has polysaccharide cell walls

• Do not accumulate starch

• Non-motile microalgae (non flagellate)(Boussiba et al., 1987)

Morphology

• Not distinguishable by morphologicalcharacteristics, only by molecular technique.

• Marine environment, but other species are alsofound in fresh water and brackish water

(Fawley and Fawley, 2007)

• Contain pigments; Chl α, β-carotene,xantophylls, violaxanthin and vaucherxantinbut lack chl β. (Cohen 1999)

Location of sampling

Teluk Batik Beach

Marina Cove Resort

Titi Panjang

(Lumut Jetty)

Sample Location Google GPS coordinate

1 Teluk Batik Beach N 4o 11’ 20.4”, E 100o 36’ 20.8”

2 Marina Cove Resort N 4o 12’ 48.06”, E 100o 36’ 08.1”

3 Titi Panjang (Lumut Jetty) N 4o13’ 56.0”, E 100o 38’ 31.6”

Location Coordinates

Table 1 : Location and coordinates of microalgae sampling.

A

B

C

Figure 1 : Growth of microalgae in F2 medium. A: 1 day, B: 4 days, C: 6 days

Figure 3 : Growth of microalgae in F2 medium after subculture (8 days)

Figure 2 : Nannochloropsis oculata Observation on microscope at

1000x magnification.

Day 1

Day 2

Day 3

Day 4

Day 5

Day 6

Day 7

Day 8

Day 9

Day 10

Day 11

Day 12

1. Hibberd, (1981). Notes on the taxonomy and nomenclature ofthe algal classes Eustigmatophyceae and Tribophyceae(Synonym Xanthophyceae). Botanical journal of the Linneansociety, 82: 93-119.

2. Boussiba, S., Vonshak, A., Cohen, Z., Avissar, I., and Richmond,A. (1987). Lipid and biomass production by the halotolerantmicroalga Nannochloropsis salina. Biomass, 12: 37-47.

3. Fawley, K. P., and Fawley, M. W. (2007). Observations on thediversity and ecology of freshwater Nannochloropsis(Eustigmatophyceae) with descriptions of new taxa. Protist, 158(3) : 325 -336.

4. Cohen, Z., (1999). Chemicals from microalga, Taylor andFrancis, London, UK.

References