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Biodiesel: Renewable fuel produced from biological oils. Microalgae are known to accumulate 20-50% triglycerides (DW). Transesterification Oil + Alcohol --> Fatty acid alkyl ester (FAME) Catalyst (Acid/ Base)
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NAME : VIJENDREN KRISHNAN
SUPERVISOR : PROF. DR. YOSHIMITSU UEMURA
CO-SUPERVISOR : DR. NURLIDIA MANSOR
DATE : 22 SEPT 2014
BIODIESEL PRODUCTION BY
SIMULTANEOUS EXTRACTION AND
CONVERSION OF TOTAL LIPIDS FROM
MICROALGAE, CYANOBACTERIA, AND
WILD MIXED-CULTURES
PAPER REVIEW
Biodiesel: Renewable fuel produced from biological
oils.
Microalgae are known to accumulate 20-50%
triglycerides (DW).
Transesterification
Oil + Alcohol Fatty acid alkyl ester (FAME)
Catalyst (Acid/ Base)
INTRODUCTION
Type Strains Media Source
Marine microalgae Chaetoceros gracilis Artificial seawater UTEX*
Marine microalgae Phaedactylum tricornutum Artificial seawater UTEX*
Marine microalgae Tetraselmis suecica Artificial seawater UTEX*
Freshwater
microalgaeNeochloris oleoabundans Bristol Media UTEX*
Freshwater
microalgaeChlorella sorokiniana Bristol Media UTEX*
Cyanobacteria Synechocystis sp pcc 6803 BG -11 Hu et al., 2000
Cyanobacteria Synechocystis elongatus BG-11 Hu et al., 2000
EXPERIMENTAL STRAINS
* Culture collection of algae from the University of Texas, Austin.
Lipid extracted using organic solvent
Solvents removed by distillation
TG transesterified
CONVENTIONAL OIL
EXTRACTION
DIRECT TRANSESTERIFICATION
100mg lyophilized biomass
Reacted with MeOH + H2SO4
Reaction stopped by adding chloroform
Phase separation by adding water 5mL , centrifuged
Residual biomass recovered with 2mL chloroform
TOTAL LIPID ANALYSIS
TAG CONTENT DETERMINATION BY GAS
CHROMATOGRAPHY
FAME CONTENT ANALYSIS BY HAS
CHROMATOGRAPHY
ANALYSES
RESULTS AND DISCUSSION
RESULTS AND DISCUSSION
RESULTS AND DISCUSSION
Direct transesterification is applicable to
diatoms, green algae, cyanobacteria, and a
wild mixed culture
High biodiesel yield.
CONCLUSION
THE END
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