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Overview of Blastocyst Culturing System in Assisted Reproductive Technologies. Dr. Minh N. Ho, Board Ceritified in Reproductive Endocrinology @ www.ivfdoc.com
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To blast or not to blastTo blast or not to blast
Blastocyst culture and Blastocyst culture and transfertransfer
Minh N. Ho, M.D., Minh N. Ho, M.D., F.A.C.O.G.F.A.C.O.G.
XPERT FERTILITY CAREXPERT FERTILITY CAREOf CaliforniaOf California
www.ivfdoc.comwww.ivfdoc.com
Board Certified in Reproductive Endocrinology
OutlineOutline
► OverviewOverview► Why blastocysts ?Why blastocysts ?► How ?How ?► Plus and minusPlus and minus
BlastocystBlastocyst DevelopmentDevelopment
Blastocyst DevelopmentBlastocyst Development
Ovulation InductionOvulation Induction
►2 cell theory2 cell theory►Agonist or antagonistAgonist or antagonist►Flare or not flareFlare or not flare►Patient selectionPatient selection►Embryo transferEmbryo transfer►Luteal supportLuteal support
Criteria for blastocyst c&tCriteria for blastocyst c&t
►Number of embryoesNumber of embryoes►Quality of embryoesQuality of embryoes►Age FactorAge Factor►Uterine FactorsUterine Factors►Reproductive historyReproductive history
Blastocyst CultureBlastocyst Culture
► Co-cultureCo-culture► Blastocyst mediaBlastocyst media
Plus and MinusPlus and Minus
► Reduce multigestationReduce multigestation► Increase implatationIncrease implatation► Not for everyoneNot for everyone► Issue with Issue with
cryopreservationcryopreservation
Ecetera…Ecetera…
►Spontaneous monozygotic twinsSpontaneous monozygotic twins►Sex ratioSex ratio► ICSI ‘ed blastocystsICSI ‘ed blastocysts►2- step ET2- step ET►Day 5 or day 6?Day 5 or day 6?
What’s nextWhat’s next
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