Git Diagnostic Tests

Diagnostic Procedures in GIT Diseases: Diagnostic Procedures in GIT Diseases:

Introduction:Introduction:

• Major cause of morbidity & mortality.• 10% of GP consultations are for indigestion.• ¼ of GP consultations for diarrhea.• Infective diarrhea is a major cause of ill heath & death in developing countries.• GIT is one of the most common sites for cancer.• Major advances had occurred in the field of GE;• PUD proved to be an infective condition due to HP & Nobel prize had been

given recently to its discoverer, Marshal.• Molecular events in the CRC development had been discovered & from this

effort became successful in its prevention by NSAIDs.• GIT endoscopy made diagnosis of GIT diseases very easy.• Therapeutic endoscopy made it possible to replace surgery for many GIT

conditions as GI bleeding, bilairy stone removal & stenting, palliative cancer stenting, polyp removal, PEG & endoscopic mucosal resection.

History:History:

• Dysphagia: difficult swallowing• Odynophagia: painful swallowing.• Aphagia: can not swallow.• Heart burn.• Non cardiac chest pain.• Regurgitation.• Aerophagia: eructation.• Hematemesis.• Melena.• Hematochesia: fresh bleeding per rectum.• Dyspepsia: abnormal digestion.• Anorexia.• Flatulence.• Alteration in bowel habits.• Bleeding per rectum.• Abd pain.

Physical exams:Physical exams:

Diagnostic Procedures in GI Diseases

• The gastrointestinal system includes the GI tract and its associated glands

• Esophagus• Stomach• Small intestine• Colon• Liver & Biliary tree• Pancreas

Diagnostic Procedures in GI Diseases

• The diagnostic tests can be divided into several categories:

• Structural tests• Functional tests• Tests for Helicobacter pylori• Special blood tests• Special stool tests

Diagnostic Procedures in GI Diseases

Structural Tests

Diagnostic Procedures in GI Diseases

Structural Tests

• Radiography• Ultrasonography• Nuclear Isotope Scanning • Magnetic Resonance Imaging• Gastrointestinal Endoscopy• Endoscopic Ultrasonography

Plain X Ray:

• Show gas within bowel for diagnosis of Int obst if there are dialated loops or fluid levels in the erect position.

• Soft tissue of the liver, spleen& kidneys & calcifications in these organs, pancrease,blood vessels, LNs,calculi.

• Chest XR in erect position show air under diaphragm in perforated viscus.

Contrast studies:

• Barium & double-cnotrast barium using air with barium, will show filling defects, strictures, erosions & ulcers & even motlity disorders if under fluroscopy.

Tests of structure: Imaging• Contrast studies: uses & limitations.

Indications Major uses LimitationsBa swallow:

Dysphagia,rfelux,chest pain,motility disorder

Stricture, HH,GERD,Achalasia.

Aspiration risk

Poor mucosal detail

No biopsy

Ba meal:

Dyspepsia,epigastric pain,vomiting,anemia,perforation.

DU,GU,Gastric Ca,GOO,

Gastric emptying disorder.

Low sensitivity for early cancer.

No biopsy

No HP assessment.

Ba follow through:

Diarrhea,abd pain, Obst by stricture

Malabsorption.

Chrons.

Time consuming.

Radiation exposure.

Ba enema

Abn bowel habit,Rectal bleeding,anemia,abd pain

Ca, diverticuli,stricture,megacolon.

Difficult in elderly.

incontinance.,

No biopsy.

Tests of structure: Imaging• U/S,CT,MRI:Increasingly used for abd diseases,

noninvasive & offer detailed images of abd contents.

Indications Major uses LimitationsUS: Masses,abscess,organs,ascites,bil

iary dilation,gall stones,guided needle aspiration & biopsy.

Low sensitive for small ls.

Little functional info.

Operator dependent.

Gass & obesity interfer with it.

CT: Pancreatic dis,liver tumor deposits, tumor staging, vascular lesions.

Expensive,high radiation, may underestimate stage of cancers as esophagus.

MRI: Tumor stage,MRCP,pelvic/perineal, chrons fistula.

Time consuming.

Clusterphobia,role not fully established,limited availability.

Tests of structure: endoscopy• Endoscpy: UGI, LGI, Enteroscopy, ERCP,EUS , Double balloon endoscopy, capsule video endoscopy.• Increasingly used for abd diseases, noninvasive & offer detailed images of abd contents.

Diagnostic Procedures in GI Diseases

Functional Tests

Diagnostic Procedures in GI Diseases

Functional Tests

• Tests for motility• 24 hour pH monitoring • Tests for acid output• Tests for malabsorption• Tests for pancreatic function

Gastrointestinal Endoscopy

Gastrointestinal Endoscopy

• Direct method to examine and biopsy the mucosal lining of the gastrointestinal tract

• Various accessories are available to take biopsies and provide therapy

Gastrointestinal Endoscopy

Types of Gastrointestinal Endoscopy

Gastrointestinal Endoscopy

Types of Gastrointestinal Endoscopy

• Esophagogastroduodenoscopy (Upper GI Endoscopy)

• Small Bowel Enteroscopy (Jejunoscopy)

• Colonoscopy (Lower GI Endoscopy)

• Sigmoidoscopy

• Endoscopic Retrograde Cholangiopancreatogram (ERCP)

Upper Gastrointestinal Endoscopy

• Heartburn

• Dysphagia or odynophagia

• Hematemesis or melena

• Dyspepsia or upper abdominal pain

• Unexplained weight loss or anemia

• Evaluation of abnormal Barium meal X-ray

• Suspected malabsorption

• Control of bleeding

• Dilation of stricture

• Removal of foreign bodies

• Removal of polyps

• Tumor ablation

Diagnostic Indications Therapeutic Indications

Upper Gastrointestinal Endoscopy

Contraindications to Upper GI Endoscopy

• Uncooperative patient

• Hemodynamically unstable patient: Recnet AMI, Unstable angina or arrhythmia,Severe resp dis.

• Suspected perforation

• Severe RA of the cervical spine

• Severe shock.• Atlanto axial sublaxation.• These may be relative in experienced hands.

Upper Gastrointestinal Endoscopy

Examples of therapeutic techniques in endoscopy.

Upper Gastrointestinal Endoscopy

Normal Esophagus Normal Stomach Normal Duodenum

Duodenal UlcerGastric UlcerEsophagitis

Lower Gastrointestinal Endoscopy

• Chronic diarrhea• Rectal bleeding• Iron deficiency anemia• Unexplained abdominal pain• Constipation, change in bowel

habits or stool caliber• Unexplained weight loss• Evaluation of abnormal Barium

enema x-ray• Personal or family history of

colon cancer• Personal history of IBD

• Control of bleeding

• Removal of polyps

• Tumor ablation

• Dilation of stricture

• Colonic decompression

• Reduction of sigmoid volvulus

Diagnostic Indications Therapeutic Indications

Lower Gastrointestinal Endoscopy

Contraindications to Lower GI Endoscopy

• Uncooperative patient

• Hemodynamically unstable patient

• Suspected perforation

• Suspected colonic obstruction

• Suspected diverticulitis

• Soon after a myocardial infarction

Lower Gastrointestinal Endoscopy

Lower Gastrointestinal Endoscopy Normal Colon Colon Cancer

Colon Polyp and Polypectomy

Endoscopic Retrograde Cholangiopancreatogram

Indications

Endoscopic Retrograde Cholangiopancreatogram

Indications

• Obstructive jaundice (benign or malignant)

• Ascending cholangitis • Gallstone pancreatitis• Unexplained jaundice or

elevated LFT’s• Bile duct injury or leak after

cholecystectomy• Chronic pancreatitis• Pancreatic cancer• Suspected Sphincter of Oddi

dysfunction

Endoscopic Retrograde Cholangiopancreatogram

Gallstone impacted at ampulla, sphincterotomy being done and stones removed

Endoscopic Retrograde Cholangiopancreatogram

Pancreatic cancer with dilated bile duct and pancreatic duct (Double Duct sign)

Endoscopic Ultrasound

• The ultrasound probe is placed at the tip of the endoscope

• Allows ultrasonography of organs from a close distance

• Allows close evaluation of the bowel wall

• Can be used to take fine needle aspiration samples from adjoining regions/organs

Endoscopic Ultrasound

A T3 Rectal Tumor on EUS

Diagnostic Procedures in GI Diseases

Capsule Endoscopy

Capsule Endoscopy

Capsule Endoscopy

• Capsule endoscopy is intended for visualization of the small bowel mucosa

• It may be used as a tool in the detection of abnormalities of the small bowel in adults and children from 10 years of age and up

Diagnostic Indications

Capsule Endoscopy

• Capsule endoscopy is contraindicated for use under the followingconditions:

– In patients with known or suspected gastrointestinal obstruction, strictures, or fistulas based on the clinical picture or pre-procedure testing and profile

– In patients with cardiac pacemakers or other implanted electromedical devices

– In patients with swallowing disorders

Contraindications

Diagnostic Procedures in GI Diseases

Functional Tests

Diagnostic Procedures in GI Diseases

Functional Tests

• Tests for motility• Tests for pH • Tests for acid output• Tests for malabsorption• Tests for pancreatic function

Functional Tests in GI Diseases

Tests for motility

Functional Tests in GI Diseases

Tests for motility

• Esophageal Manometry

• 24 Hour pH Monitoring

• Anorectal Manometry

Functional Tests in GI Diseases

Indications for Esophageal Manometry

• Oropharyngeal dysphagia with normal structural studies- Cricopharyngeal achalasia, Pharyngeo-UES dyscoordination

• Esophageal dysphagia with normal structural studies - Primary esophageal body dysmotility e.g. Achalasia, Diffuse esophageal spasm, Nutcracker esophagus, Hypertensive LES, Collagen vascular disease, Amyloidosis, Hypothyroidism, etc.

• Noncardiac chest pain

Functional Tests in GI Diseases

Esophageal Manometry Tracings

Esophageal Manometry

Functional Tests in GI Diseases

24 Hour pH Monitoring

Indications

• Patients with non cardiac chest pain

• Refractory acid reflux symptoms: evaluate treatment efficacy

• Pre and post-operative evaluation of antireflux surgery

• Patients with atypical presentations of acid reflux (ENT, pulmonary)

Functional Tests in GI Diseases24 Hour pH Monitoring

Functional Tests in GI Diseases

Anorectal Manometry

• Used in the clinical assessment of patients in whom a problem with defecation is suspected

• This technique is helpful in evaluating the anorectal sphincter mechanism

• Contraindicated in the presence of an anal fissure, since the resting anal pressure is abnormally high

Functional Tests in GI Diseases

Anorectal Manometry

Tests of structure: Biopsy• Obtained through endoscpy or percutanously & sent for histopath exam.• Reasons for biopsy or cytological exams:• Brash cytology of suspected malignant lesions.• Histological assessment of mucosal abns.• Diagnosis of infections( candida, HP,Giardia).• Measure enzymes as disacharidases.• Analysis of genetic mutations as oncogenes , tumor suppressor genes.

2.Tests of infection: Bacterial cultures• For identifying causes of diarrhea sp if acute or bloody.• Causes of infective diarrhea:• Viruses: Rota, adeno, entero, requires EM or viral cultures.• Bacteria: Campylo jej, EC,Salmonella,clostridium difficile( ned toxine

isolation).• Protozoa: Giardia,ameba, cryptosporidium & moicrospora.

Functional Tests in GI Diseases

Tests for Malabsorption

Functional Tests in GI Diseases

Tests for Malabsorption• Serum screening tests for malabsorption

S. Calcium, Albumin, Iron, Vitamin B12, Folate, Carotene, Prothrombin time

• Quantitative fecal fat determination• D-xylose absorption test• Small bowel biopsy• Schilling test for Vitamin B12

absorption• Hydrogen Breath Test for lactose intolerance

Diagnostic Procedures in GI Diseases

Liver Biopsy

• Liver biopsy is a diagnostic procedure used to obtain a small amount of liver tissue which can be examined under a microscope to help identify the cause or stage of liver disease

• The most common way a liver biopsy is obtained is by inserting a needle into the liver percutaneously

• Other ways to biopsy the liver are transjugular, laparoscopic and surgical

• In case of a localized lesion in the liver a US or CT guided biopsy is performed

Diagnostic Procedures in GI Diseases

Diagnostic Procedures in GI Diseases

Diagnostic Procedures in GI Diseases

Tests for Helicobacter pylori

Diagnostic Procedures in GI Diseases

Tests for Helicobacter pylori

Non-invasive• C13 or C14 Urea Breath Test• H. pylori IgG titer (serology)• Stool antigene for H Pylori.

Invasive• Gastric mucosal biopsy• Rapid Urease test

Diagnostic Procedures in GI Diseases

Tests for Helicobacter pyloriC13 or C14 Urea Breath Test

Diagnostic Procedures in GI Diseases

Tests for Helicobacter pyloriMucosal Biopsy

Diagnostic Procedures in GI Diseases

Tests for Helicobacter pylori

Rapid Urease Test

• This test is based on the urease enzyme present in the H. pylori

• Urea is split into NH3 and CO2

• The change in pH causes a color change in the medium

Diagnostic Procedures in GI Diseases

Blood tests

• Liver function tests (LFT’S)

• Hepatitis serology

• S. Amylase & Lipase

• Alfa-Feto Protein (AFP)

• Carcino-Embryonic Antigen (CEA)

Stool tests

• Stool microscopy

• Stool ova & parasites

• Stool culture

• Stool C. difficile toxin

• Stool occult blood

Diagnostic Procedures in GI Diseases

Diagnostic Procedures in GI Diseases

Diagnostic Procedures in GI Diseases

Diagnostic Procedures in GI Diseases

Diagnostic Procedures in GI Diseases

Diagnostic Procedures in GI Diseases

Diagnostic Procedures in GI Diseases

Diagnostic Procedures in GI Diseases

Diagnostic Procedures in GI Diseases

Diagnostic Procedures in GI Diseases