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Fungi Propagation for DIY Domestic Mycologists
@Gil_Lopez, Cofounder Smiling Hogshead Ranch
Brooklyn Botanic Garden
Fall 2015
PRESENTATION OUTLINE
• Caution in Identification &
Harvesting
• Kingdoms
• Mushroom Anatomy & Lifecycle
• Three Classes of Fungi
• Cultivating Mushrooms at Home
• Cardboard Demo & Hands-on
• Supply List & Instructions
• Milk Crate Demonstration
THIS IS NOT A MUSHROOM IDENTIFICATION CLASS
There are lots of ways to ID Mushrooms
including:
Smell it
Touch it
Make a Spore Print
Look for a Cup, a Ring or Warts
Look at the Shape of the Cap
Look at How the Gills Attach to the Cap
Look at the Shape of the Stem
Look at How the Stem Emerges from the Cap
Look at the Colors and Markings
Look Through a Microscope
SOME MUSHROOMS ARE TOXICNever eat any wild fungi you cannot ID 100% correctly
Honey Mushroom = Delicious Death Cap = Deadly
FIVE KINGDOM CLASSIFICATION
Evidence strongly
suggests that animals &
fungi are sister groups
while plants evolutionary
lineage diverged over 1.1
billions years ago.
Fungi and Animals break
down and their own food
sources.
Animals and Fungi share
4 unique protein
sequences in their DNA.
MUSHROOM ANATOMYFungal Fruit & Mycelium
Fru
itin
g B
od
y
My
celi
um
Hyphae
Hyphae
Underground Hyphae
Stalk
Cap
Gills
MUSHROOM ANATOMYFungal Parts
Scale
Tubes
Pores
Stipe/Stalk
Scales
Cap
Gills
Ring
Volva
MUSHROOM LIFECYCLESexual & Asexual Reproduction
TYPES OF FUNGI
MutualistsParasites
Saprophytes
MUTUALISTICTypes of Fungi
Mycorrhizae grow on plant
roots, expanding surface area
and digesting nutrients. It can
also transfer photosynthesized
nutrients from plant to plant.
Lichens are a symbiotic relationship
between fungi and photosynthesizing
organisms (like algae).
PARASITICTypes of Fungi
Corn Smut Athlete’s FootCordyceps
SAPROTROPHYTICTypes of Fungi
Mold on bread
Mushrooms on wood
Mushrooms on
coffee grinds
MAKING CARDBOARD MUSHROOM SPAWN
Materials List:
Water
Scissors or knife
Corrugated cardboard
Mushroom stem butts
of: Oyster, Garden
Giants, Turkey Tails or
primary decomposers.
Small container with
fabric cover
http://www.mushroom-appreciation.com/mushroom-spawn-cardboard.html
MILK CRATE FUNGI PROPAGATIONUpcycling urban waste products to grow healthy mushrooms at home
SUPPLY LIST
Gloves & Rubbing alcohol
Starter Kit/Spawn
Spent Coffee Grinds
Sawdust/wood shavings
Milk Crate
Cardboard
Trash Bag
Knife
SUBSTRATE INOCULATION
Gather materials
Sterilize tools
Prepare your
workstation
Mix the materials
SPAWN RUN
Put your inoculated substrate in an environment with the recommended conditions for colonization (e.g., 75° F forPleurotus ostreatus)
Allow the substrate to become fully colonized by the mushroom mycelium. (called “the spawn run”)
When the little mushrooms (called “primoidia”) first appear (called pinning), put the substrate with primoidia in an environment with the recommended conditions to promote fruiting. This will mean controlling temperature, light, humidity, and air flow that your chosen species likes.
Harvest your mushrooms! Ideally, just before spores are released.
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