HS-SDME

Analysis of Nitrosodiethylamin and Nitrosodiprophylamin in Sausage using Single Drop

Microextraction Coupled with GC

Zarah Nur Intissar

(080710150)

Counsellor : Reviewer :

1.Dra. Aning Purwaningsih, M.Si. Dra. Usreg Sri Handajani, M.Si

2.Yanuardi Raharjo, S.Si., M.Sc.

Introduction

CHAPTER I

BACKGROUND

Food Preservative

Nitrite Carcinogen

NitrosamineNitrosodiethylamine

(NDEA)Nitrosodiprophylamine

(NDPA)

Sausage

NitrosamineLow Concentration

( ppb )Sample preparation

(extraction)

Single Drop Microextraction

(SDME)

Direct-SDME

Headspace-SDME

BACKGROUND

PROBLEMS OF RESEARCH

o Are the Nitrosodiethylamine and Nitrosodiprophylamin can be analyzed using HS-SDME?

o How the optimation result of speed of squealer, volume of organic phase and time of extraction for HS-SDME applicated to the analysis of NDEA and NDPA?

o Are the HS-SDME can be used for analysis of NDEA and NDPA in sausage?

OBJECTIVE OF RESEARCH

o To analysis of NDEA and NDPA using HS-SDME.

o To determine the optimum for speed of squealer, volume of organic phase and time of extraction for anaysis NDEA and NDPA using HS-SDME.

o To know that the result of optimum parameters can be used for analysis of NDEA and NDPA in sausage.

BENEFIT OF RESEARCH

To produce a new method to the analysis NDEA and NDPA quickly, selective and cheap in sausage.

To know the concentration of nitrosamine in sausage so that we can anticipation for the negative effect from nitrosamine.

BAB II

Tinjauan Pustaka

• Karsinogenik• Nitrit yang bereaksi pada

kondisi sangat asam• Salah satu senyawa

turunan nitrosamin yakni NDEA dan NDPA

Nitrosamin

• Makanan favorit• Pembuatan sosis• Macam sosis yakni sosis

mentah dan sosis siap makan

Sosis

Single-Drop Microextraction

(SDME)

Direct-SDME

Headspace-SDME(HS-SDME)

Drop pelarut organik di rendam langsung di dalam

larutan sampel

Drop pelarut organik di letakkan di atas

larutan sampel (menggantung)

Gas Chromatography (GC)

Jenis kromatografi untuk memisahkan dan menganalisa campuran zat

Komponen instrumen kromatografi gas

BAB III

Metode Penelitian

ALAT DAN BAHAN PENELITIAN

Alat Penelitian :

•Gelas beaker 25 ml

•Botol kaca 15 ml

•Botol kaca 2 ml

•Botol kaca 100 ml

•Mortar

•GC

•Microsyringe 25 µl

•Mikropipet 1000 µl

•Tube mikropipet

•Labu ukur 10 ml

•Shaker

•Saringan

Bahan Penelitian

•Metanol 70 %

•NDEA 99,99 %

•NDPA 99,99 %

•Sosis

•n-heksan

SKEMA KERJA

Syringe berisi pelarut organik

Jarum syringe

Larutan pengekstraksi (drop pelarut organik)

Pengaduk magnetik

Larutan Standar/sampel

Proses Ekstraksi HS-SDME

Setelah proses ekstraksi selesai, pelarut organik ditarik ke dalam syringe kemudian diinjeksikan ke GC

Botol kaca

Penutup