Wild Type Drosophila

Wild Type Drosophila

Ommatidium

8 Photoreceptor cells (R1-R8)4 Cone cells (secrete lens)8 Pigment cells

Wild Type Eye Phenotype

Eyeless Mutant Phenotype (hypomorphic allele)

DNA

Paired Domain Protein

Figure 1A

LacZ

No Transcription of LacZ

Gal4 Enhancer-Trap Method

Figure 1A

LacZ

Enhancer that controls expression in specific tissues

Gal4 Enhancer-Trap Method

Figure 1A

LacZ

Tissue-specific Expressionof Gal4

Gal4 Enhancer-Trap Method

Tissue-specific Expressionof LacZ

Figure 1

Eye-Antennal disc wing disc leg disc

Figure 1A

LacZ

Gal4 Enhancer-Trap Method

Enhancer that controls expression in imaginaldisc cells

Transcription of LacZ in imginal disc cells

Figure 1A

Enhancer that controls expression in imaginaldisc cells

Transcription of eyeless in imginal disc cells

Figure 2

Figure 3

DNA

Paired Domain Protein

Drosophila ey, mouse Pax-6 (sey) and Human Pax-6 (Aniridia) genesare ~ 94% similar in Paired domain

Enhancer that controls expression in imaginaldisc cells

Transcription of Pax-6 in imginal disc cells

Pax-6

Ectopic Expression of Mouse Pax-6 Gene

Figure 5

Ectopic Expression of Mouse Pax-6 Gene

Aniridia -- Human Pax-6 Mutation

Absence of the iris

Xenopus Oocyte

Animal Pole

Vegetal Pole

Xwnt-11 mRNA Localization

Xwnt-11 is Drosophila wingless homogue

Normal

Ventralized

Embryo Grafting Experiment

Goosecoid ProteinExpression

Embryo Grafting Experiment

InjectmRNAs

ForChordin etc.

Formation of ventral structures

Normal

Dorsalized