Western Blotting

Complete and Innovative Western Workflow Solutions Western Blotting

Separate tranSfer Detect

In the United StatesFor customer service call 1-800-766-7000To fax an order use 1-800-926-1166To order online wwwfisherscicom

In CanadaFor customer service call 1-800-234-7437To fax an order use 1-800-463-2996To order online wwwfisherscica

The NuPAGEreg SDS-PAGE Gel Systemsimulates the denaturing conditions of the traditional Laemmli systems

(Tris-glycine SDS-PAGE gels) NuPAGE gels use a unique buffer formulation

to maintain a neutral operating pH during electrophoresis minimizing the

ldquosmilesrdquo and poor resolution seen with Tris-glycine SDS-PAGE gels

NuPAGE Bis-Tris and Tris-acetate gels also offer

bull Superior protein band resolution and stability

bull Faster sample run times at 35 to 50 minutes

bull Long ambient product shelf life - 16 months

bull More efficient western blot transfer

Product Quantity Cat NoSeparateMini Gel Tank 1 unit A25977Boltreg Welcome Pack 10 well 1 kit NW0412ABoltreg Welcome Pack 15 well 1 kit NW0412BMagicMarktrade XP Protein Standard Varies VariesNativeMarktrade Unstained standard 5 x 50 μL LC0725PageRulertrade Unstained Low Range Protein Ladder 2 x 250 μL PI-26632PageRulertrade Unstained Protein Ladder 2 x 250 μL PI-26614PageRulertrade Prestained Protein Ladder Varies PI-26616PageRulertrade Plus Prestained Protein Ladder Varies PI-26619Spectratrade Multicolor Broad Range Protein Ladder Varies PI-26634Spectratrade Multicolor High Range Protein Ladder Varies PI-26625HiMarktrade Pre-stained Protein Standard 250 μL LC5699Boltreg Bis-Tris Plus Precast Gels Varies VariesXCell4 SureLocktrade Midi-Cell 1 each WR0100NuPAGEreg Bis-Tris Precast Gels Varies VariesNuPAGEreg Tris-Acetate Precast Gels Varies VariesNovexreg Tris-Glycine Precast Gels Varies VariesPowerEasereg 90W Power Supply (115 VAC) 1 each PS0090PowerEasereg 300W Power Supply (115 VAC) 1 each PS0300Separate and transferBoltreg Welcome Pack with iBlotreg 2 Dry Blotting System 1 kit NW0412AIB2Mini Gel Tank and Blot Module Set 1 kit NW2000

The Mini Gel Tank is designed for more intuitive use and convenience compared to traditional electrophoresis tanks

bull Versatilemdashcompatible with NuPAGEreg

Boltreg or Novexreg gels

bull Easy sample loadingmdashwith forward-facing

well configuration

bull Simultaneous visualization of both gelsmdashstreamlined side-byside tank configuration

bull Simplified monitoring of prestained protein markersmdashwith white tank stand

bull Less running buffer requiredmdashtwo separate gel chambers so you only need to load

sufficient buffer for each gel to the specified fill line

The XCell4 SureLocktrade Midi-Cell allows simultaneous vertical electrophoresis of 1 to 4 midi

gels without leaking enabling consistent performance It

uses proprietary technology to make electrophoresis easier

and more reliable than ever and is designed to dissipate

heat effectively and evenly and enable high-resolution

results when using Novexreg midi gels

For more information visit wwwfisherscicomlife or wwwfisherscicalife

Western Blotting

14_1957 LWlp 1114 BN1113142

Transferbull Mini Blot Wet Transfer Module

for seamless transfer in the Mini Gel Tank fits inside the tank and requires less methanol-based transfer buffer

bull Thermo Scientifictrade Piercetrade Power Blotter designed specifically for rapid semi-dry transfer of 10 to 300 kDa proteins from polyacrylamide gels to nitrocellulose or PVDF membranes in 5 to 10 minutes

bull iBlotreg 2 Dry Blotting System for self-contained reproducible and flexible gel transfer in only 7 minutes compatible with multiple gel chemistries membrane types and gel sizes

DetectThe iBindtrade Western System is an automated western processing device Itrsquos hands-free reproducible and compatible with all western detection protocols

Primary and Secondary Antibodies and ConjugatesPolyclonal antibodies for many species of IgG and other immunoglobulins offered in purified (unconjugated) forms and as conjugates with HRP AP biotin and popular fluorophores Fisher Scientific offers market leading antibodies such as Thermo Scientific BD Biosciences and RampD Systems

Browse antibodies

Additional Products

SubstratesChemiluminescent HRP substrates for excellent performance in western blotting Thermo Scientifictrade Piercetrade ECL Piercetrade ECL 2 and SuperSignaltrade Chemiluminescent Substrates deliver high sensitivity long signal duration strong signal intensity and low background

Product Quantity Cat No

Piercetrade ECL Substrate 500mL kit PI-32106

Piercetrade ECL 2 Substrate 100mL kit PI-80196

SuperSignaltrade West Pico Chemiluminescent Substrate 500mL kit PI-34080

SuperSignaltrade West Dura Extended Duration Substrate 200mL kit PI-34076

SuperSignaltrade West Femto Maximum Sensitivity Substrate 200mL kit PI-34096

myECLtrade Imager for one-touch image capture and analysis of western blots a powerful and easy-to-use blot and gel documentation instrument for sensitive multimode image capture and analysis that works with chemiluminescent colorimetric or UV lightndashactivated fluorescent substrates or stains

Western Blotting

14_1957 LWlp 1114 BN1113142

Transferbull Mini Blot Wet Transfer Module

for seamless transfer in the Mini Gel Tank fits inside the tank and requires less methanol-based transfer buffer

bull Thermo Scientifictrade Piercetrade Power Blotter designed specifically for rapid semi-dry transfer of 10 to 300 kDa proteins from polyacrylamide gels to nitrocellulose or PVDF membranes in 5 to 10 minutes

bull iBlotreg 2 Dry Blotting System for self-contained reproducible and flexible gel transfer in only 7 minutes compatible with multiple gel chemistries membrane types and gel sizes

DetectThe iBindtrade Western System is an automated western processing device Itrsquos hands-free reproducible and compatible with all western detection protocols

Primary and Secondary Antibodies and ConjugatesPolyclonal antibodies for many species of IgG and other immunoglobulins offered in purified (unconjugated) forms and as conjugates with HRP AP biotin and popular fluorophores Fisher Scientific offers market leading antibodies such as Thermo Scientific BD Biosciences and RampD Systems

Browse antibodies

Additional Products

SubstratesChemiluminescent HRP substrates for excellent performance in western blotting Thermo Scientifictrade Piercetrade ECL Piercetrade ECL 2 and SuperSignaltrade Chemiluminescent Substrates deliver high sensitivity long signal duration strong signal intensity and low background

Product Quantity Cat No

Piercetrade ECL Substrate 500mL kit PI-32106

Piercetrade ECL 2 Substrate 100mL kit PI-80196

SuperSignaltrade West Pico Chemiluminescent Substrate 500mL kit PI-34080

SuperSignaltrade West Dura Extended Duration Substrate 200mL kit PI-34076

SuperSignaltrade West Femto Maximum Sensitivity Substrate 200mL kit PI-34096

myECLtrade Imager for one-touch image capture and analysis of western blots a powerful and easy-to-use blot and gel documentation instrument for sensitive multimode image capture and analysis that works with chemiluminescent colorimetric or UV lightndashactivated fluorescent substrates or stains

Western Blotting - Fisher Scientific...• iBlot® 2 Dry Blotting System for self-contained,...

Documents

Fig. 20-11 TECHNIQUE Nitrocellulose membrane (blot) Alkaline solution DNA transfer (blotting) Sponge Gel Heavy weight Paper towels Preparation of restriction

Blotting Techniques

Protein Blotting Guide - Wolfson Centre Home Pagewolfson.huji.ac.il/.../BIORAD_ProtBlottingGuide.pdf · Protein Blotting Guide Theory and Products ... western blotting (immunoblotting),

WESTERN BLOTTING Lab. 7. Introduction Blotting is a technique by which a macromolecule such as DNA, RNA, or protein is resolved in a gel matrix, transferred

Western Blotting Handbook and Troubleshooting … 1 Tel: 800-874-3723 or 815-968-0747 The term “blotting” refers to the transfer of biological samples from a gel to a membrane

Phyto85n11 1449 - APS Home › publications › phytopathology › back...gel electrophoresis followed by Southern blotting. Agroinoculation and analysis of plants infected with the

Chapter 6 -Molecular Genetic Techniques · 06/04/2017 · Molecular genetic technologies! •DNA gel electrophoresis •Southern blotting •DNA sequencing •Automation •Northern

Kelompok 6 Kimia B (Southern Blotting dan Northern Blotting)

THIS IS YOUR PRESENTATION TITLE - fac.ksu.edu.sa · Disadvantage of Northern blotting. 3. WESTERN BLOTTING . Western blotting, also known as immunoblotting“ or protein blotting,

iBlot 2 Dry Blotting System - Thermo Fisher Scientifictools.thermofisher.com/content/sfs/manuals/iblot2_device_man.pdf · 2 Dry Blotting System User Guide 7 System overview The iBlot

Blotting techniques1

Macromolecule Blotting - e-PGPathshalaepgp.inflibnet.ac.in/epgpdata/uploads/epgp_content/S000002BI/P... · Macromolecule Blotting Blotting Southern Northern Western. 5 Macromolecule

Northern blotting. Electrophoresis We can separate DNA and RNA molecules by size using agarose gel electrophoresis

iBlot - AIEprogram P3 for 7–8 minutes. using the iBlot® Gel Transfer device with Blotting Roller Instructions are provided below to assemble the iBlot® Gel Transfer Stack to perform

iBlot Dry Blotting System Online Specials · Part no. 25-0912 MAN0003281 iBlot® Dry Blotting System Cat. no. IB1001, IB1001EU, IB1001UK Rev. Date: 2 December 2011 Instructions for

Western blot · 3. Place one sheet of blotting paper on top of sponge. Make sure it wet evenly and completely 4. Place the SDS gel onto the surface of the blotting paper 5. Place

Simply Better - bts-biotech.com€¦ · Enjoy an ultra user friendly pre-cast horizontal Polyacrylamide Gel Electrophoresis system. Featuring outstanding resolution and blotting sensitivity

Northern blotting

PCR, Gel Electrophoresis, and Southern Blotting