Water structures carrying DNA information, Application to HIV/AIDS and Autism L.Montagnier,...

Water structures carrying DNA information,

Application to HIV/AIDS and Autism

L.Montagnier, Borovets, October 23, 2013

The Evolution of Medicine

Toward 4 P Qualities

• P1 Preventive

• P2 Predictive

• P3 Personalized

• P4 Participative

Emergence or reemergence of new epidemics due to :

Globalization of exchanges and travels Demography: concentration in large cities Nutrition (pesticides, water) Environmental factors Climate changes, electromagnetic radiations Contacts with wild and farm animals Decline of immune defenses

- Cancers- Cardiovascular- Neurodegenerative- Arthritic- Autoimmune- Multifactorial, but in common:

oxidative stressinfectious agents (?)

- Cancers- Cardiovascular- Neurodegenerative- Arthritic- Autoimmune- Multifactorial, but in common:

oxidative stressinfectious agents (?)

The most important burdenChronic Diseases

Radiations (, X, UV, visible, hertzian)

Air chemical pollution

Inadequate Food

Excessive physical exercize

tobacco smoke

alcohol

ischemia

Parasitic infections

Bacterial infections

Viral infections

Various environmental factors effects accumulate Various environmental factors effects accumulate

+++++

++

+

= DISEASE+

Lipid hyperoxydation (plasma, cell membranes)

Protein Oxydation --> destruction, agregates

DNA oxydation --> Mutations, Chromosomal

Breakage

Pathological effects of Oxydative Stress

Oxidative stressWeakens the immune system

Activates transcription factors(NF-kappa B)

Activates genes involved in cell division, inflammatory cytokines,

lymphocytes activation

Immune dysfunction, apoptosis(TH1 TH2)

The Human Genome

Genes (+ introns +promoters) 3%Retroelements: retroviruses 8%

retrotransposons 13%LTR 23%Total 47%

Repeats 50%LINE 22%SINE 13%

Unknown ?

Mucosa

Skin

Mucosa

Skin

Our microbial ecosystem :

Constant exposure to microbial agents and immune protection

Extreme genetic plasticityof microorganisms:

virus : HIV, Influenza Bacteria (plasmids) Parasites (retrotransposons)

against reaction of the immune system

- Biofilms- Mobile antibiotic resistant genes- Nanoforms- Nanostructures- Endosymbiontes

- Biofilms- Mobile antibiotic resistant genes- Nanoforms- Nanostructures- Endosymbiontes

How bacteria have learned how to persist despite the immune system

Parasite Bacterium Virus

(Worm) intracellular

Filarial nematode Wolbachia

Persistent cold infections

Antibiotics affect them only when they go out of sanctuaries

•Inability of the immune system to eradicate them (tolerance, oxidative stress)•Non-multiplicative forms of « classical » bacteria•intracellular bacteria •Sanctuaries(bone marrow, joints, intestine, brain, ….)•Vectors (Parasites) •Remote effects (toxin, nanostructures)

EXPLORING THE ROLE OF LATENT INFECTION IN CHRONIC DISEASESA physical and molecular approach

Two technologies for detecting bacteria and viral DNA’s

•A new technology based on the production of electromagnetic waves

•The Polymerase Chain Reaction (PCR) using the 16S ribosomal DNA variability

oligo

oligo

Taqpolymerase

Classical model of PCR

Resonance emission of low frequency electromagnetic waves by high water dilutions

of DNA.

A newly discovered property of DNA :

Molecular recognition

without close contact(by waves and resonance)

Capture of the signals

Amplifier Computer

X 500 Signal Analysis software

Sensor coil

Sample

Detection of Ultra Low Frequencies Waves (ULF 500-2000 hertz) in certain dilutions of filtrates (100nM, 20nM, 15nM) from cultures of micro-organisms (virus, bacteria) or from the plasma of humans infected with the same agents.

Detection of Ultra Low Frequencies Waves (ULF 500-2000 hertz) in certain dilutions of filtrates (100nM, 20nM, 15nM) from cultures of micro-organisms (virus, bacteria) or from the plasma of humans infected with the same agents.

FACTS

2ng/1ml 10-2 10-3 10-4 10-5 10-6 10-7 10-8 10-9 10-10 10-12 10-13 10-14 10-15 10-16

Filtration 0.1

0.02

7-100 Hz

1000 -3000 Hz

Time (sec)

Am

plitu

de

Noise

(+)

Spectral Frequency Analysis

Fourier Tranformation

A positive signal is defined by:

amplitude increaseShift to higher frequencies (500-2000 Hertz)

Noise

(+)

Frequency (1-20000 Hertz)

Tim

e (s

ec)

Suspensions of pure culture of bacteria with pathogenic potential are producing electromagnetic signals (EMS) in the range of dilution usually 10-8 10-13, sometime (E.coli) up to 10-18

They are: E.coli, Streptococcus, Salmonella, Staphylococcus, B.subtilis, Clostridium, Pseudomonas, etc.

Micro-organisms involved in EMS induction

1.DNA from main pathogenic bacteriaStreptococcusStaphylococcusPseudomonasMycoplasma pirumSalmonellaClostridiumProteus mirabilisB. SubtilisBorrelia burgdorferiSutterella

- From viruses HIV1Influenza group AHBVHCV

- Genes involved M.pirum adhesinHIV genes

Free polymerself maintained

by EMS

Water excitated statecrystal like gel

no EMS

10-2 10-9

DNA

Interpretation

I – DNA’s emit EMS

II – EMS are produced by water nanostructures (naneons)

III – EMS are producing naneons

IV – Naneons and EMS carry specific DNA information

EMS are produced by water nanostructures (naneons)

Evidence :

from filtration

Size : between 20 and 100 nM for bacterial sequences

Smaller that 20µM for viral sequences

from biophysical studies

indicating spectrometral changes in the dilutions

producing EMS

Naneons and EMS carry

specific DNA information

Natural and digital transmission

oligo

oligo

Taqpolymerase

Classical model of PCR

PCR on water nanostructuresoligo

Taq

oligo

EMS

DNA Water

Naneons

EMS EMS

DNA Water

NaneonsPCR

7 Hz

-6

µmetal

Generator 7Hz

+ + +

-2 -3 -4 -5 -6

18hrs

water-6

Tube 2

water

EMS

Tube 1

DNA

D-4 LTR HIV DNA (104bp) 7Hz, 18 Hrs and then PCR (35 cycles) from D-2 to D-15 after filtration 450 and 20 nM

Transmission in water of D-4 LTR HIV DNA (104bp) 7Hz, 18 Hrs and then PCR (35 cycles) from D-2 to D-15 after filtration 450 and 20 nM

DW: Distilled Water / FD2: Dilution 10-2 after filtration 450and 20 nM

DNA Water Naneons

EMS

DNA

Water Naneons

PCR

ComputerDigitized

Receiver

ComputerEMS Analog

Water-mediated photonic transmission of DNA

Reproduction of DNA transduction

in other laboratories

File EMS of 194 bp DNA from HIV1 LTR

Sent to Benevento University,

Molecular Biology Laboratory

DNA reproduced and sequenced

100 % identical to original

File EMS of 499 bp DNA from Borrelia burgdorferi

Sent to Laboratory of Chronix Biomedicals

University of Gottingen

Reproduction of DNA transduction in other laboratoriesFile EMS of 194 bp DNA from HIV1 LTRSent to Benevento University,Molecular Biology LaboratoryDNA reproduced and sequenced100 % identical to originalFile EMS of 499 bp DNA from Borrelia burgdorferiSent to Laboratory of Chronix BiomedicalsUniversity of Gottingen

Water-mediated photonic transmission of DNA

Gel electrophoresis of the PCR DNA product (Borrelia Burgdorferi)E.Schutz et al. Goettingen, 2011

How pathogenic information can persist, and escape immune defence and treatment.

DNA

DNA

DNA

DNA

DNA

WATER(plasma)

ELF

- The viral DNA reservoir- The Higher sensitivity of African people to HIV infection

- The viral DNA reservoir- The Higher sensitivity of African people to HIV infection

Two main problems for AIDS researchin 2013

The dual origin of EMS in HIV/AIDS

EMS plasma RBC

HIV < 20 nM ++ +/-

Agent X < 100 nM - ++

Evidence that the agent X is a bacterium

- Presence of a sequence close to 16 S ribosomal DNA of Rickettsiales- Sensitive to antibiotics ( in vitro and in vivo)- Growth in cell line- Electron microscopy- Associated with Red Blood Cells

Human sequences associated with the agent X

237 bp (human chromosome 1)213 bp (human chromosome 7)

and more…

Human sequences associated with the agent X

237 bp (human chromosome 1)213 bp (human chromosome 7)

and more…

Strong correlation with HIV infection

- Contains human DNA sequences emitting EMS only in pathogenic HIV infection

-Sequence 213 bp from Chromosome 7

EMS in HIV + patients

no EMS in HIV - patients

Risk factor 2RBC

Risk factor 1RBC

HIV plasmaRBC

DNAPCR EMS+ +

++

-+

RISK FACTORS OF HIV INFECTION

(all HIV) (20 nM)

(213/237 bp) (100 nM) (HIV+) ( all HIV+, a few HIV-)

( all HIV+, many HIV-) (HIV- & HIV+) (100 nM)

(LTR 195 bp)

« « A new intracellular bacteriumA new intracellular bacterium has has been found in human red blood been found in human red blood cells. A variant bearing extra DNA cells. A variant bearing extra DNA sequences is present in all HIV sequences is present in all HIV infected patients and also in some infected patients and also in some healthy HIV negative African healthy HIV negative African individuals”individuals”..

« « A new intracellular bacteriumA new intracellular bacterium has has been found in human red blood been found in human red blood cells. A variant bearing extra DNA cells. A variant bearing extra DNA sequences is present in all HIV sequences is present in all HIV infected patients and also in some infected patients and also in some healthy HIV negative African healthy HIV negative African individuals”individuals”..

CONCLUSION 1

CONCLUSION 2

Origin of EMS :

not necessarily depending on DNA sequence

- depending on transmissible modification of DNA (free radicals of water ?)

- Association with pathogenicity

AUTISM :

THE GUT, BLOOD and BRAIN MICROBIAL CONNECTION

GUT BLOOD BRAIN

20 -

30 -

10 -

40 -

% E

MS/

nois

e

50 100 133270 - days

Correlation between EMS, antibiotic treatmentand clinical signs in an autistic child

AZITHROMYCIN CEFUROXIM

Acc

ept

vari

ou

s fo

od

Mo

re p

rese

nt

wit

h

Fam

ily

mem

ber

s

Sta

rts

spea

kin

g

Environmental factorsgenetic

susceptibility

immunosuppression

Oxidative stress

Bacterial agents

Oxidative stress

« prion » effect

Less and less reversible

Reversible

Somatic mutations

In press

Electromagnetic detection of HIV DNA in the blood of AIDS patients treated by antiretroviral therapy.

Luc MONTAGNIER†×^, Jamal AISSA†, Claude LAVALLEE×, Mireille MBAMY , Joseph VARON⁰ #, and Henri CHENAL⁰

× World Foundation for AIDS research and Prevention (UNESCO), 1 rue Miollis, 75015, Paris, France† Nanectis Biotechnologies, France

⁰ CIRBA Centre Intégré de Recherches Biocliniques d’Abidjan, Ivory Coast# The University of Texas Health Science Center, Houston, USA.

Abstract : Electromagnetic signals of low frequency have been shown to be durably produced in aqueous dilutions of the Human Imunodeficiency Virus DNA. In vivo, HIV DNA signals are detected only in patients previously treated by antiretroviral therapy and having no detectable viral RNA copies in their blood. We suggest that the treatment of AIDS patients pushes the virus towards a new mode of replication implying only DNA, thus forming a reservoir insensitive to retroviral inhibitors. Implications for new approaches aimed at eradicating HIV infection are discussed.Key words: DNA, Electromagnetic signals, bacteria 

Centre Intégré de Recherche Bioclinique d’Abidjan

World Foundation of AIDS Research and PreventionR. Olivier, Cl. Lavallee,H.Chenal, M. Mbamy,

Nanectis Biotechnologies SAJ.Aissa, Cl.Lavallee, R.Olivier

Goettingen University and Chronix BiomedicalsE. Schutz, H.Urnovitz

University of Washington, SeattleGerald H. Pollack

“Absence of evidence is not

evidence of absence”

Carl Sagan