Varian 900-LC Series i HPLC. 2 What is the i HPLC? 2 models 920-LC and 940-LC Compact, stylish,...

Varian 900-LC Series iHPLC

2

What is the iHPLC?

• 2 models 920-LC and 940-LC

• Compact, stylish, automated analytical and semi prep HPLC

• Pre-plumbed and factory tested for the highest confidence

• Easy to order, install and use

– Single Ethernet cable connection

• Focused on improved productivity through automation

• Updated premium performance hardware

• Autosampler, Fraction Collector and Scale up Module for semi-prep

• Quaternary pump, precision Column Heater,RI, Fluor, ELSD and autosampler for analytical

• Updated Integrated Instrument Software Interface

• Eliminate repetition, modernise UI

• Consistent

• Easy to use with added functionality

• Adds to modular range

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Varian 920-LC Integrated Analytical HPLC

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Varian 920-LC What Is It?

• Compact, stylish automated analytical HPLC

– Pre-plumbed and factory tested for the highest confidence

– Single cable communications

• Virtually pulse-free dual piston pump

– Does not require a pulse damper

• State-of-the-art low pressure quaternary gradient module with built-in degasser

• Autosampler with cooling option

– Synchronised injection for outstanding retention time reproducibility

– Compatible with 200 x 1.6 ml HPLC vials or 3 microplates

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Varian 920-LC What Is It?

• Either dual UV-Vis or PDA detector

– Optional second detector - Fluorescence or RI

• High precision (<0.15oC) dual column heater available

– Low delay solvent pre-heater for improved heat transfer

– Optional column switching and electric injection valve

• NEW improved instrument interface on GalaxieTM software

• External CVM, ELSD or 356-LC RI can be plugged directly into the back of the unit.

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Varian 920-LC Silver Bullets

• Premium analytical pump performance and reliability

• Superb Fast LC performance optimised for sub 3 um columns

– Improved throughput without compromising robustness

• High sensitivity/top line performance detection systems across UV/PDA/RI/Fluor and ELSD – unparalleled strength

• Unbeatable Injection performance and carryover statistics with 2008 loopless technology autosampler

• Full system level computer controlled AIQ (validation)

• Updated driver for enhanced user experience

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920-LC from Conventional to Fast HPLC

25cm x 4.6mm Pursuit XRS 5um 1.0ml/min

25cm x 4.6mm Pursuit XRS 5um 2.2ml/min

15cm x 4.6mm Pursuit XRS 5um 1.0ml/min

15cm x 4.6mm Pursuit XRS 5um 3.75ml/min

10cm x 2.0mm Pursuit XRS 2.8um Ultra 0.8ml/min

5cm x 2.0mm Pursuit XRS 2.8um Ultra 0.4ml/min

5cm x 2.0mm 2.4um 1.3ml/min16 x Speed Enhancement

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Varian 920-LC Fast LC PAHs

Varian 920-LC6 minute Analysis

Waters Acquity7 Minute Analysis

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Varian 920-LC Solvent Delivery System

• Dual piston quaternary pump produces fast gradients – even at 200 l/min

• Virtually pulse-free pumping without pulse damper

• Outstanding gradient compositional accuracy give retention time precision at the <1% level

• Built-in vacuum degas maintains pump prime and enhanced injection reliability

• Accurate flow from 0.1 to 10 mL/min for 2 to 4.6 mm columns

• Overlay of 10 chromatograms

• 10% steps at 1ml/min

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Varian 920-LC Autosampler

• Needle-in-flow path geometry for low carry-over (<0.01%)

• Typical inj precision (< 0.3% at 10 l)

• Optimized flow paths for fast injection turn-around

• Synchronised injection for best RT reproducibility

• Programmable wash setting for low carry-over

• Injection volumes from 1ul to 50L

• Cooled option available

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Varian 920-LC Washout Performance

Blank injection <0.01% carryover

125 ug/ml Caffeine

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Varian 920-LC Injection Synchronization

-20%

0%

20%

40%

60%

80%

100%

120%

0 0.2 0.4 0.6 0.8 1 1.2 1.4 1.6 1.8 2 2.2

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Sensitive UV-Vis Detectors

Typical Agilent VWD S/N ~ 25

Typical Varian 920-LC UV/Vis S/N > 36

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Information Rich Diode Array Detection

• 1024 pixels provides full coverage from 190 – 950 nm

• Full spectra collection• Enhanced purity

• Automatic peak identification with customised libraries• Greater confidence on compound identification

• Choice of flowcells• 4 mm path length semi-micro • 9mm path length analytical

• Front panel access• Lamps and flowcells

• D2 and Vis lamps self aligned• Easily user maintained

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Information Rich Diode Array Detection

• Automatic peak identification with customised libraries• Use spectra as well as RT for compound ID

• Greater confidence on compound identification

• Peak search of unknown yields 998.7 match for naphthalene

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Varian 920-LC Fluorescence Detector

• Sensitive Fluorescence detector (S/N > 600 Raman H2O peak baseline method)

• Time programmable for increase sensitivity• Automatic validation with built-in Hg lamp• Low 12 l dead volume flowcell for improved

chromatography

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Varian 920-LC Refractive Index Detector

• Based upon 356-LC RI detector• Low volume, flow efficient cell for superior peak shape• Stable, long life and high intensity LED

• Minimises drift

• Provides excellent detection limits

• Enhanced life-time

• Temperature controlled optics minimises baseline drift and provides outstanding detection limits

• Complete automation thru Galaxie software

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Varian 380-LC and 385-LC ELSD

• Universal HPLC detector• Compatible with gradients

• More sensitive than RI

• NEW Glass Nebuliser for Improved Reproducibility• 2 to 3 Times More Sensitive

• 40Hz Data Rate for Fast LC• Complete Galaxie compatibility

• Program changes thru a run

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Heated Column Compartment

• Temperature range from ambient + 10oC up to 65oC

• Excellent temperature precision +0.15oC

– Ensures retention time reproducibility

– Eliminates re-runs

• Good temperature accuracy <1oC

– Low delay volume pre-heater for efficient heat transfer to mobile phase

• Rapid warm-up

– Typically from ambient to 40oc in <15 minutes

• Spacious - easily accommodates 2 x 250mm columns and 2 valves

• Option with electrically actuated column switching valve and injection valve

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Excellent Long term Stability

20

22

24

26

28

30

32

34

36

38

40

42

44

46

48

50

11:4

6:00

12:2

2:00

12:5

8:00

13:3

4:00

14:1

0:00

14:4

6:00

15:2

2:00

15:5

8:00

16:3

4:00

17:1

0:00

17:4

6:00

18:2

2:00

18:5

8:00

19:3

4:00

20:1

0:00

20:4

6:00

21:2

2:00

21:5

8:00

22:3

4:00

23:1

0:00

23:4

6:00

0:22

:00

0:58

:00

1:34

:00

2:10

:00

2:46

:00

3:22

:00

3:58

:00

4:34

:00

5:10

:00

5:46

:00

6:22

:00

6:58

:00

7:34

:00

8:10

:00

8:46

:00

9:22

:00

Time

Te

mp

era

ture

(d

eg

ree

s C

)

Mobile phase input Temp

Mobile phase temp inside column

Oven Temp

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Quick Warmup - Outstanding Stability

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22

24

26

28

30

32

34

36

38

40

42

44

8:00

:00

8:10

:00

8:20

:00

8:30

:00

8:40

:00

8:50

:00

9:00

:00

9:10

:00

9:20

:00

9:30

:00

9:40

:00

Time

Te

mp

era

ture

(d

eg

ree

s C

)

Ambient Room

Column heater

Column

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Reproducibility of 50 Injections

Reproducibility <0.8% over 50 injections and 12 hours

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Varian 920-LC Software Usability

• User friendly GalaxieTM software

• Simplified, modern, consistent Instrument interface

• Modular 11 pages

• 920-LC 4 pages!!

• One page real-time status and chromatogram

• Advanced integration and calibration

• New IMS capabilities

• Automated instrument validation

• Reduces downtime and operating costs

• Fusion AE

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Varian 920-LC Integrated HPLC

Special features of the Varian 920-LC• Leak detector

• Increases confidence with unattended automated runs

• Single waste point• Ensures capture of all wastes

• Easy to connect to waste vessel

• Built-in Solvent bottle storage• Improved safety and convenience

• Lower hight than modular systems

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Varian 920-LC Integrated HPLC

Special features of the Varian 920-LC• Clear front doors

• Stylish

• Provides access to autosampler without opening

• Safety increased as lab staff shielded from solvent spray and leaks

• Useful, convenient storage compartments• Column rack in door

• Tool drawer

• Operation log drawer

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Varian 920-LC AIQ (Validation)

AIQ (Analytical Instrument Qualification)• Fully PC controlled IQ and OQ (not PQ)

• Puts the Varian 920-LC at the forefront of regulated capabilities

• Consistent, reproducible methodology• Includes pumps, autosampler, detectors, column

heater

• Chargeable option

• Fully documented

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900 LC -Series AIQ

• Full system test designed and implemented as part of the 900-LC Series Analytical Instrument Qualification package.

• Verifies the entire LC system by systematically running the following

• Flow Rate Accuracy Test

• Wavelength Detector Accuracy Test

• Auto-sampler Injector Accuracy Test (also measures Detector Linearity)

• Auto-sampler Injector Precision Test

• Auto-sampler Injector Carryover Test

• Proportioning Accuracy Test

• Detector Noise Test

• Detector Drift Test

• Auto-sampler Vial Identification Accuracy Test

• Proportioning Linearity

• Compositional Ripple

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Varian 920-LC Configurations

pump inject detector Oven Detector 2

option

Options

Isocratic

00-100890-00Dual piston pump 10 ml head

Auto Dual UV/Vis

Opt FL, RI ELSD

Gradient

00-100891-00Quat, Grad, Degas Auto Dual

UV/VisOpt FL, RI ELSD opt

High Performance Gradient

00-100892-00

Quat, Grad, Degas Auto cooled Dual UV/Vis

Opt FL, RI ELSD opt

Development

00-100893-00Quat, Grad, Degas Auto PDA Std FL, RI ELSD opt

Research

00-100894-00Quat, Grad, Degas Auto cooled PDA Std FL, RI ELSD opt

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Varian 920-LC Consumables

• Excellent range of HPLC Columns providing industry leading resolution and robustness

• Pursuit

• Pursuit XRS

• Polymeric Phases - PL

• From 2mm to 2” ID Prepacked

• Other Speciality Phases

• NEW 2.8 Ultra Column AND 2.4um Columns for Fast or High Res LC

• 2.8um Ultra is optimised for Speed

• 2.4um gives further enhancements in Resolution

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2.8um Vs 2.4 um – High Res or Extra Speed

2.8um 2mm x 5cm for speed

2.4um 3mm x 5cm for RESOLUTION

Questions??