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it;s about tuberculin test
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TUBERCULIN TEST
AMAN MAURYA
ROLL NO.-10
INTRODUCTION• HISTORY• ROBERT KOCH PREPARED A PROTEIN
EXTRACT OF TUBERCLE BACILLUS BY
CONCENTRATING TENFOLD BY EVAPORATION
, A 6-8 WEEK CULTURE FILTRATE OF THE
BACILLUS GROWN IN 5% GLYCEROL BROTH.
THIS WAS CALLED “ORIGINAL” OR “OLD
TUBERCULIN”
• FIRST USED FOR ALLERGING TESTING BY
VON PIRQUET (1906)
ROBERT KOCH
VON PIRQUET
PRINCIPLE
• RESPONSES
ANTITUBERCULAR IMMUNITY
TUBERCULIN HYPERSENSTIVITY
BOTH RESPONSES MEDIATED BY T-
LYMPHOCYTE SENSITISED TO THE
BACTERIAL ANTIGEN .
REAGENTS
• OLD TUBERCULIN (OT)
• ROBERT KOCH
• 6-8 WEEK CULTURE
• CONCENTRATED BY EVAPORATION ON A
HEATED WATER BATH.
• NOW RARELY USED
REAGENTS PURIFIED PROTEIN DERIVATIVE (PPD)
SEIBERT (1941)
Prepared by M.tuberculosis In semisynthetic
medium
1 TU = 0.01 ml of OT
MANTOUX Test-
0.1 ml of PPD is injected intradermally
into flexor aspect of forearm.
METHOD
METHODHEAF Test-
• multiple puncture apparatus
• A drop of undiluted PPD is spread on the
area of skin
• The apparatus is pressed against this
area of skin.
RESULT
In MANTOUX test the site of injection is
examined after 48-72 hrs
RESULT
Positive test-
local oedema- 10 mm diameter
confirms past infection
helpful in children under 5 years
indicate active infection
becomes positive 4-6 weeks after
infection or BCG vaccination.
FALSE negative
Miliary tuberculosis
Infection of measles
Hodgkin”s disease
Sarcoidosis
Lepromatous leprosy
Malnutrition
Administration of immunosuppressive
agents and corticosteroids
RESULT
RESULT
FALSE positive
atypical mycobacteria
USES To measure prevalence of infection in a
community
To diagnose active infection in young
children
It is used as an indicator of successful
BCG vaccination
Recommended