Top producing beamlines of the world

Top producing beamlines of the world

0

20

40

60

80

100

120

140

160

180

AP

S 19ID

AL

S 8.3.1

ES

RF

ID14-1

ES

RF

ID14-4

PF

(all 5)

ES

RF

ID14-2

NS

LS

X4A

AP

S 22ID

ES

RF

ID29

AP

S 19B

M

CH

ES

S (all 4)

AL

S 8.2.1

AL

S 5.0.2

HA

SY

LA

B X

11

HA

SY

LA

B B

W6

AL

S 8.2.2

SL

S (b

oth

)

ES

RF

BM

14

SP

RIN

G8 26B

1

AP

S 14B

MC

NS

LS

X25

AL

S 5.0.1

SS

RL

BL

9-1

NS

LS

X12C

20022003

2004

http://asdp.bnl.gov/asda/Libraries/pdb_statis/latest/bml/ALL.html

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Top producing beamlines of the world

0

0.51

1.5

22.5

3

3.54

4.5

5

AP

S 19ID

ES

RF

ID14-4

ES

RF

ID14-1

ES

RF

ID29

HA

SY

LA

B B

W6

AL

S 8.3.1

ES

RF

ID14-2

AP

S 22ID

AP

S 14B

MC

SL

S X

06SA

AL

S 8.2.1

AP

S 19B

M

NS

LS

X4A

SS

RL

BL

9-1

HA

SY

LA

B X

11

AL

S 8.2.2

SR

S 14.2

NS

LS

X25

SP

RIN

G8 26B

1

ES

RF

BM

14

AL

S 5.0.2

SS

RL

BL

11-1

MA

XL

AB

I711

NS

LS

X12C

NS

LS

X8C

20022003

2004

http://asdp.bnl.gov/asda/Libraries/pdb_statis/latest/bml/ALL.html

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Top producing beamlines of the world

0

200

400

600

800

1000

1200

1400

1600

1800

2000

NS

LS

X26C

NS

LS

X12C

AP

S 14B

MD

NS

LS

X8C

NS

LS

X4A

NS

LS

X12B

AL

S 5.0.1

NS

LS

X9B

AL

S 5.0.3

SS

RL

BL

9-1

AP

S 14B

MC

SS

RL

BL

7-1

AL

S 5.0.2

SS

RL

BL

1-5

AL

S 8.3.1

SS

RL

BL

9-2

AL

S 8.2.1

SS

RL

BL

11-1

AP

S 14ID

B

NS

LS

X25

AP

S 19B

M

AL

S 8.2.2

CH

ES

S F

2

CH

ES

S F

1

CH

ES

S A

1

AP

S 17B

M

NS

LS

X9A

AP

S 17ID

AP

S 19ID

20022003

2004

http://asdp.bnl.gov/asda/Libraries/pdb_statis/latest/bml/ALL.html

http://biosync.sdsc.edu/

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s

Number Description Percent

446028 Images (~7 TB) 33%

2346 Data sets 47%

449 MAD/SAD (1:2) 19%

48 Published 2%

8.3.1 in 2003

Beamline Efficiency

Seconds Description Percent

104490 Assigned and available 91%

42093 Shutter open 40%

52684 Collecting (3026 images) 50%

51806 Something else 50%

Beamline Efficiency“representative” 8.3.1 user

Seconds Description Percent

51806 Something else 100%

247s 45

Mounting 22%

229s 37

Centering 16%

179s109

Strategizing 38%

309s 37

Prepping 24%

Beamline Efficiency“representative” 8.3.1 user

Seconds Description Percent

16820 Something else 100%

10s 45 Mounting 3%

30s 37 Centering 7%

140s109

Strategizing 90%

0s 37 Prepping 0%

Beamline Efficiency“expert” user

Touch screen

Traffic light

firewire

DVD data archive

Beamline pager

Ice-B-gone

Foam dewars

Foam dewars

Zero-parallax optics

pinhole

prism

microscope

backstop

Zero-parallax optics

pinhole

prism

microscope

backstop

Zero-parallax optics

pinhole

prism

microscope

Styrofoam™ backlight

backstop

Zero-parallax optics

pinhole

prism

microscope

Apr 6 – 24 at ALS 8.3.1

Elven Automation

27,686 images collected

148 datasets (15 MAD)

31 investigators

56 unique cells

5 KDa – 23 MDa asymmetric unit

0.94 – 32 Å resolution (3.2 Å)

Apr 6 – 24 at ALS 8.3.1

Elven Automation

148 datasets

117 succeded

~3.5 (0.1-75) hours

31 failed

~61 (0-231) hours

2 / 15 MAD structures

Number Description Percent

446028 Images (~7 TB) 33%

2346 Data sets 47%

449 MAD/SAD (1:2) 19%

48 Published 2%

8.3.1 in 2003

Beamline Efficiency

Hampton Pin

Syrrx Pin

plastic Pin

Yale Pin

SuperPin

SuperTongSuper Tong

Hampton PinSuper Tong

Syrrx PinSuper Tong

plastic PinSuper Tong

Yale PinSuper Tong

SuperTong

thaw

Radiation Damage

Incremental strategy

incremental_strategy.com merged.mtz auto.mat

Incremental strategy

incremental_strategy.com merged.mtz auto.mat

OTIS

shoot nextsample

OTIS

Expe

rim

ent

recentered snapshot at 0º put away worse than 2A molecular replacement using 1ZTA

using 1 Å x-rays detector at 2 Å resolution

OTIS

Expe

rim

ent

recentered snapshot at 0º put away worse than 2A molecular replacement using 1ZTA

using 1 Å x-rays detector at 2 Å resolution

OTIS

Expe

rim

ent

recentered snapshot at 0º put away worse than 2A molecular replacement using 1ZTA

using 1 Å x-rays detector at 2 Å resolution

OTIS

Expe

rim

ent

recentered snapshot at 0º put away worse than 2A molecular replacement using 1ZTA

using 1 Å x-rays detector at 2 Å resolution

OTIS

Expe

rim

ent

recentered snapshot at 0º put away worse than 2A molecular replacement using 1ZTA

using 1 Å x-rays detector at 2 Å resolution

OTIS

Expe

rim

ent

recentered snapshot at 0º put away worse than 2A molecular replacement using 1ZTA

using 1 Å x-rays detector at 2 Å resolution

OTIS

Expe

rim

ent

recentered snapshot at 0º put away worse than 2A molecular replacement using 1ZTA

using 1 Å x-rays detector at 2 Å resolution

OTIS

Expe

rim

ent

recentered snapshot at 0º put away worse than 2A molecular replacement using 1ZTA

using 1 Å x-rays detector at 2 Å resolution

OTIS

Expe

rim

ent

recentered snapshot at 0º put away worse than 2A molecular replacement using 1ZTA

using 1 Å x-rays detector at 2 Å resolution

OTIS

Expe

rim

ent

recentered snapshot at 0º put away worse than 2A molecular replacement using 1ZTA

using 1 Å x-rays detector at 2 Å resolution

OTIS

recentered snapshot at 0º put away worse than 2A molecular replacement using 1ZTA

using 1 Å x-rays detector at 2 Å resolution

OTIS

recentered data set from 50º to 150º with 1º oscillations using 1 Å x-rays detector at 2.5 Å resolution