TILED DATA RECONSTRUCTION AND CORRECTION

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TILED DATA RECONSTRUCTION AND CORRECTION

ASHWINI NIKKAM

JITHU JOSESHRAVYA RAO

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INTRODUCTIONWhen a large biological specimen

has to be pictured in high resolution, it must be done in tiles. 

Tiles must be fit or stitched together to form one image. 

Overlap  between each tile and its neighbors.

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OUR GOAL

OLYMPUS Data Conversion

Accurately stitch multiple images

Correct the brightness differences across the image

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BIO-FORMAT IMPORTER

Bio-format Importer is a plugIn to load images into Fiji or ImageJ.

Opens proprietary formats that contains batches of images and converts into OME data model.

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OLYMPUS DATA CONVERSIONApproach: A custom plugIn that

calls the Bio-formats Importer PlugIn

The custom plugIn launches the Bio-format importer to select and read the images

Converts and saves images in .tiff format

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OLYMPUS DATA CONVERSION PLUGIN

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OLYMPUS DATA CONVERSION

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STITCHING OF IMAGESStitching PlugIn is used to

reconstruct big images from arbitrary number of tiled input images.

Stitching has two plugIns:1.Pairwise stitching2.Grid/Collection stitching

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STITCHING OF IMAGESPairwise stitching –Stitches, loads

two images loads and saves to hard disk.

Grid/collection stitching- Stitches an arbitrary collection or grid images(2d,3d,4d,5d) of same type .

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STITCHING OF IMAGES

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STITCHING OF IMAGES

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STITCHING OF IMAGES

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CORRECTING BRIGHTNESS

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CORRECTING BRIGHTNESS

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REFERENCES

http://fiji.sc/Bio-Formats

http://fiji.sc/Image_Stitching

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THANK YOU