The Use of Animals in Research Is a Privilege We Wish to Retain Animal Welfare Versus Animal Rights...

The Use of Animals in Research Is a Privilege We Wish to Retain

Animal Welfare Versus Animal Rights

How are Animal Welfare Concerns Reported?

Regulatory Oversight

APHIS1: Animal Welfare Act

NIH: Guide for the Care and Use of Laboratory Animals

AAALAC2: Ongoing Accreditation Inspections

Inspectors Select Animals and their Protocols for Review

Their Review is Based on What is Written

1. Animal and Plant Health Inspection Service (USDA)

2. Association for Assessment and Accreditation of Laboratory Animal Care

Animal Protocols

A Research Facility Must Have an Institutional Animal Care and Use Committee (IACUC)

An IACUC Approved Animal Protocol Is Required Before Any Animal Experiments Can Be Requested Or Begun

New NIH Just In Time Process

Who Can Apply for Animal Protocols at TSRI ?

Research staff (Professors)

Research Associates, with the Professor taking responsibility if an appointment to the research staff is pending on funding

The applicant must have completed all appropriate training

Animal Protocol Review

Protocol Review

Full Committee Review – Monthly

Designated Reviewer – Ongoing

Review Depends on Protocol Content

The Steps Involved

Forms, Instructions, Standard Protocols Booklet in the new IACUC Office in the 3366 Building. A website is coming soon!

The Assurance Declaration to be Signed by the Institutional Official once the Protocol is approved by the IACUC

The Title Page

Boxes to be checked may signal need for full committee review

Collaboration within or out of TSRI (to be explained in the protocol)

1. List the number of animals requested.

2. Provide the experimental plan and use of procedures with scientific justification.

Justify the species and number of animals requested. The use of statistics is recommended by the PHS to determine group size.

Provide information on veterinary care (standard statement)

Protocols involving surgery need veterinary consultation during preparation

Measures to minimize pain and distress including post-operative monitoring and analgesia

Euthanasia

Must conform with the AVMA 2000 Panel Report on Euthanasia or be justified scientifically

Alternatives Statement for Painful or Distressful Procedures:

Include data bases searched (Medline and Agricola a minimum), key words, date of search, must consider the 3 R’s: reduction, refinement, and replacement

Library/resources/animal alternatives web site

No unnecessary duplication must also be assured

General description of the project and its scientific justification

Scientific American style, so lay people on the Committee can understand

Can modify the summary statement from the grant

Antibody production

CFA alternatives statement

Use of Standard Protocols Booklet (see example)

HANDLING (CODE: MH)A mouse should be picked up by grasping its tail close to the body, the loose skin near the nap of the neck or by gently closing your hand around the body. To restrain a mouse for examination and injections, grasp the loose skin at the nape of its neck between your thumb and index finger and place its tail between the fourth and fifth fingers. Be careful not to impede respirations or to grab the tail anywhere but close to the base; remember that the tail can be damaged if excess pressure is applied, or if grasped in areas distal to the base. Mother mice normally move their offspring by picking them up by the nape of the neck. Pain and/or distress level II.

Must provide detailed protocols for any procedures not included in or at variance with the Standard Protocols Booklet

Need to include post procedure monitoring, care, and analgesia

List all substances administered to animals including dose, route, infectious and radioactive compounds

Attach IBC, EH&S, radiation approvals as needed

Biological products, consider contamination

List any work requested outside the animal facility

Required training varies with role in protocol

Training continued

List work done by DAR

Signature of PI taking responsibility for following the protocol

Remember, the animal protocol must cover all animal work requested in the grant

When complete, a copy of the grant must be submitted to be kept on file with the protocol