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The Patient: Figaro
• What type of macromolecule is an antibody?
• What shape is a typical antibody?
Protein
• What is an antigen?
• How many antigens can the antibody below bind to?
Foreign Molecule
Figaro Activity Possible Antigens
Brain storm ways to test for possible allergies.
• Label 3 Petri Plates with initials, date, period, LB Agar, and TRIAL 1, 2 or 3 (depending on the plate).
• Cut the bottom off a large transfer pipet.
• Apply a slight suction by compressing the bulb of the pipet between your fingers.
• Use the transfer pipet to poke holes in the agar.
• Make 4 holes around the edge of the agar.
• Make 1 hole in the center of the agar.
1
2
3
4
C
• In your notebook, draw a Petri plate and label which antigen goes into each outside hole.
Allergen 1: ?
Allergen 3: ?
Allergen 2: ?
Allergen 4: ?
1
23
4
• On your Petri plate drawing in your notebook, label the center hole as Figaro’s Blood Serum.
Figaro’s Blood Serum: Antibodies
C
• Use a sterile 1 mL pipet to add antigen/ antibody solution to each hole in the agar.
• Add enough antigen serum so the solution is at the top of the agar hole.
• Use a different sterile 1 mL pipet for each antigen solution.
• Fill the center hole with Figaro’s Blood Serum (antibody solution).
• Repeat these steps for all 3 trials/ Petri plates.
• Cover all 3 Petri plates and leave undisturbed for 24 hours.
• Collect Petri plates.• Draw Petri plate results in
your notebook.• Write down observations• Form a data table in Excel.• Create numeric scale to
convert observations to data.
• Collect data from each trial.• Average the data from trials.
• A precipitin line will appear between one or more of the allergens and the blood serum.
Precipitin Lines
• Record results of each of the 3 trials using a numeric scale– 5 = strong precipitation; 0 = no precipitation
• Calculate average results for each antigen
• The brighter precipitin lines, the stronger the allergy.
Weak Allergy Strong Allergy
• This lab is an example of an Ouchterlony test.
• When antibodies diffuse in agar and bind with antigens, it causes agglutination, or a white precipitin line.
Agglutination = Precipitin Lines
• Make an individual data table in Google documents.
• On the Smart Board, add your group’s data to the table and make a class average data table on your Google spreadsheet below your individual data table.
• Post your individual data table, class average data table, and your REE PE PA three-paragraph conclusion on a new page for this lab on your Google Site.
• Results - Go back to the purposes of the lab.• Evidence - Numerical evidence.• Explanation - Go back to your hypothesis.• Possible Errors
– 2 examples of errors– Avoid future errors
• Practical Applications– Suggestions to patient/family.– Further testing– Value of this lab/test
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