The Mouse Bioinformatics Research Network (MBIRN) Cal Tech- Russ Jacobs Duke- G. Allan Johnson Mike...

The Mouse Bioinformatics Research Network (MBIRN)

Cal Tech- Russ JacobsDuke- G. Allan Johnson

Mike FehnelUCLA- Art Toga

UCSD- Mark EllismanMaryann Martone

Goals

The unique challenges of MBIRN

Representative collaborations

The value of connections

MBIRN- a heterogeneous data collectionImages

MR,optical,EMRegulatory

IACUCC, FundingAgencyProject Description

Goals,Literature,DesignSpecimen

Age,Weight,Sex,GenotypeExperimental

TR/TE,Resolution, Special StainsAnalysis

Segmentation,volumes,visualizationBehavioral Data (future)

CollaborationsShiverer

UCLA,Cal Tech, UCSD,DukeAlpha-synuclein overexpressor

UCSD,DukeDAT/KO, Rheeler

MBIRN- MorphBIRNAstrocytoma

UCSD UNC Duke

Alpha-SYN aggregates in brains of tg mice

Diana PriceNatalie MacLean

~0.5 TB

Alpha-synuclein MouseDiana Price, UCSD

Actual Diffusion Actual Diffusion RestrictionRestriction

Tensor ModelTensor Model

Diffusion Tensor Model of Restricted, Anisotropic Diffusion

Caltech 2004 Tyszka & Jacobs

3D DTI Shiverer MouseCNS +/+

CNS shi/shi

• Naturally occurring mutant- a model of demyelating disease

• Myelin basic protein (MBP) gene deletion.

• Virtual absence of compacted myelin in CNS, but not PNS.

• Motor tremors, seizures and significantly reduced lifespan (approx 90 days).

Caltech 2004 Tyszka & Jacobs

0.0

1.0

FA

[D]

Mean Fractional Anisotropy (Control)

Caltech 2004 Tyszka & Jacobs

3D Diffusion Tensor7 sets @ 512x256x256

1.75 Gb

0.0

1.0

FA

[D]

Mean Fractional Anisotropy (Shiverer)

Caltech 2004 Tyszka & Jacobs

3D Diffusion Tensor

7 sets @ 512x256x256

1.75 Gb

Summary

Image Registration

Brain Morphology in DAT-KO MiceAki Laakso, Ph.D., Duke University

3D reconstruction of caudate-putamenand ventriclesQuickTime™ and a

Video decompressorare needed to see this picture.

P<0.001 n.s.

Two-way repeated measures ANOVA

Profile of caudate-putamen in DAT-KO mice:

Stereological cell counts

Same brains that were used for MRI

Striatum divided into anterior and posterior parts, based on MRH profile analysis

All nuclei: Neuronal nuclei:

Brain Morphology in DAT-KO MiceMichel Cyr, Ph.D., Duke University

Reeler Mouse MBIRN-MorphBIRNWilliam Wetsel, Duke

Connections

Man Mouse CelllarSubcellular

MBIRN

Morph BIRN

fMRI BIRN

NCRR Resources

International Research Community

Tools Data Ideas People

QuickTime™ and aDV/DVCPRO - NTSC decompressor

are needed to see this picture.