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Strategies for cloning How to find the clone for a specific gene?
Terms: • Direct selec:on • Different types of DNA library • Iden:fica:on from gene library • Iden:fica:on of a clone
Selec:on problem!!
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Different strategies for selec:on
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Direct selec:on
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Direct selec:on for TrpA gene
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Gene library in a cosmid
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Expression of genes
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Nucleic acid hybridiza:on
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32P-‐dATP
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Labelling different techniques
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Iden:fica:on of high abundance clones
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Heterologous probing
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Southern hybridiza:on
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Immuno screening
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