Scientific Studies and University Experiments

SCIENTIFIC STUDIES AND UNIVERSITY

EXPERIMENTS

• Following are pictures of the surgical procedures and research conducted at Mahidol University, in Thailand

• All Thai research was coordinated by Assistant Professor Kampon Kaeoket, DVM, MSc, PhD (Pig Production); Dusit Laohasinnarong, DVM, and Dr. Jo Ann Hachuela of the Philippines.

Preparation and staging of animals for research

Ovulation detection using ultrasound

Administration of pre-anesthetic drugs

Administration of anesthetic

Sedated and anesthetized animal heading for surgery

Cleaning and disinfecting the surgical site

Properly restrained animal at beginning of surgery

Ovariohysterectomy in progress

Locating the reproductive tract

Exposing the uterine horns, oviducts, and ovulated ovaries

Ovulated ovaries

Removing the reproductive tract

Transferring the tract for flushing procedures

Separating the upper uterine horns, oviducts, and ovaries

Incising the corpora lutea and determining the number of ovulated oocytes

Ovaries, upper portion of uterine horns, and oviducts ready for flushing

Incising and separating the isthmus from the ampulla and infundibulum

Pre-labeled petri dishes for the oocytes to be recovered

Withdrawal of phosphate buffer saline solution for flushing

Flushing of the isthmus

Flushing of the ampulla and infundibulum

Flushing of the upper uterine horn

Stereo microscopes for examining the oocytes

Prof. Kampon Kaeoket, D.V.M., M.S. (Obstetrics and Gynecology), Ph.D.(Obstetrics and Gynecology, Pig Reproduction) examining fertilized vs. non-fertilized oocytes

Isolating the oocytes to maintain accuracy during counting

Further examination…

Click the document to open a .PDF file showing actual evaluation protocols used for the Mahidol University Trial.

Click the document to open a .PDF file showing the surgical and on farm trial results from Thailand.