Reward-Summation Function of the Priming Effect · Reward-summation function Pulse Frequency (Hz)...

Reward-Summation Function of the Priming Effect

Esthelle Ewusi-Boisvert

Concordia University

October 28th, 2015

Objective

•Examine the effect of priming on motivation

in rats

2

Motivation

3(Kelley & Berridge, 2002)

5

Priming effect

6

Sample RewardEnhancement in

motivation

Priming effect

7

Sample RewardEnhancement in

motivation

Priming effect

8

Sample RewardEnhancement in

motivation

Background

9(Gallistel et al., 1966)

Background

10

Background

11

12

Background

13

14(Sinnamon & Stopford, 1987)

Operant box paradigm

15

Reward-summation function

Pulse Frequency (Hz)

Re

wa

rd-s

ee

kin

g p

erf

orm

an

ce 100

0

0 35016

Reward-summation function

Unprimed

Primed

17(Gallistel et al., 1974)

Pulse Frequency (Hz)

Pe

rfo

rma

nc

e

(ru

nn

ing

sp

ee

d)

100

0

0 350

Reward-summation function

Pulse Frequency (Hz)

Unprimed

Primed

Pe

rfo

rma

nc

e

(ra

te o

f b

ar

pre

sse

s)100

0

0 35018

(Gallistel et al., 1974)

Left shift

Pe

rfo

rma

nc

e (

rate

of

ba

r p

ress

es)

100

0

Pulse Frequency (Hz)0 350

19

Unprimed

Primed

Single pulse frequency

Pe

rfo

rma

nc

e (

rate

of

ba

r p

ress

es) RAT A RAT B

Pulse Frequency (Hz)

0 176 350 0 176 350

Pe

rfo

rma

nc

e (

rate

of

ba

r p

ress

es)

Pulse Frequency (Hz)

100

0

100

0

20

300 200

Our aim

•Using an operant paradigm, we will examine

the effect of electrical priming stimulation on

the reward summation function

21

Method

• Five Long-Evans rats (N=5)

•2x9 within-subjects design

•Counterbalanced conditions

22

Proposed design

23

Pulse Frequencies

Priming

Conditions

PF

1

PF

2

PF

3

PF

4

PF

5

PF

6

PF

7

PF

8

PF

9

Primed

Unprimed

Proposed design

Pe

rfo

rma

nc

e (

rate

of

ba

r p

ress

es)

Pulse Frequency (Hz) 24

Proposed design

Priming/No priming

Setup Lever

Reward

25

Proposed design

Priming/No priming

26

Proposed design

Setup Lever

27

Proposed design

Reward

28

1

2

…

Unprimed Primed … 120min.

29

Primed Unprimed … 120min.

Expected results

Unprimed

Priming

30

Pe

rfo

rma

nc

e (

rate

of

ba

r p

ress

es)

100

0

Pulse Frequency (Hz)0 350

Pulse Frequency (Hz)

Pe

rfo

rma

nc

e

(ru

nn

ing

sp

ee

d) 100

0

0 350

Data analysis

•Repeated measures ANOVA

•Post-hoc tests

31

Data analysis

Pe

rfo

rma

nc

e (

rate

of

ba

r p

ress

es)

Pulse Frequency (Hz) 32

Data Analysis

•Repeated measures ANOVA

•Post-hoc tests

33

Limitations

•Electrode placement

•ESB and specificity

34

Discussion

•Motivation

•Pulse frequency levels

• Implications for substance abuse research

35

Acknowledgements

•Dr. Wayne Brake

•Dr. Peter Shizgal

•Czarina Evangelista

•Kent Conover

• Steve Cabilio

• Sarah Nolan-Poupart

•CSBN

36

Priming and motivation

37(Deutsch et al., 1964)

Neurobiology

38

39

Relapse

40