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Progress Report on Miniaturized Liquid
Chromatography for NeSSI
Scott Gilbert, CPAC Visiting ScholarCrystal Vision Microsystems LLC
Atofluidic Technologies, LLC
Lab-on-chip approach
Simplicity of operation Reduction of moving
parts – no (mechanical) pumps
Robustness Inexpensive and facile
maintenance
Chip Layout20
mm Column
Inlet lines
Detector electrodes
From Old Setup…
…To New NeSSI Mountable Design
Split flow approach to sampling
Liters per minute
microliters per minute
nanoliters per minute
Fluid Flow Strategy
Functional Integration of -fluidic LC Chip for On-line Sample Pretreatment
micromixer
diluent in
column
mobile phase in
sample in
Rapid Prototyping Using Quick Photolithographic Method
Inkjet printed photomask Finished product
Front Mounted Microfluidic Chip
Back-Mounted Valves
Quantitation of flow splitting
Secondary Flow vs. Main Flow
0.00
0.50
1.00
1.50
2.00
2.50
3.00
0 50 100 150 200 250 300
Main Flow (ml)min)
Se
co
nd
ary
Flo
w (
ml/m
in)
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