plant transpiration

Plant transpiration

Introduction

Methodology

Summary and Conclusions

Results and discussion

Light and Photosynthesis

IntroductionMethodology

Summary and Conclusions

Results and discussion

Flowchart

Plant transpiration

Introduction

Methodology

Summary and Conclusions

Results and discussion

Light and Photosynthesis

IntroductionMethodology

Summary and Conclusions

Results and discussion

Flowchart

Plant TranspirationPlant Transpiration

Lateral MovementLateral Movement

And And

Path of Water RisePath of Water Rise

Plant transpiration

Introduction

Methodology

Light and Photosynthesis

IntroductionMethodology

Flowchart

Results and discussion

Summary and Conclusions

Results and discussion

Summary and Conclusions

DefinitionDefinition

TranspirationTranspiration

evaporation of water from plants. It occurs evaporation of water from plants. It occurs chiefly at the leaves while their stomata are chiefly at the leaves while their stomata are open for the passage of COopen for the passage of CO2 2 and Oand O22 during during

photosynthesis.photosynthesis.

Factors Affecting TranspirationFactors Affecting Transpiration

1.1. LightLight

2.2. HumidityHumidity

3.3. TemperatureTemperature

4.4. WindWind

5.5. Soil moistureSoil moisture

6.6. Internal Conc. Of COInternal Conc. Of CO22

lightlight

Plants transpire more rapidly in the light than Plants transpire more rapidly in the light than in the dark. in the dark.

light stimulates the opening of the stomata. light stimulates the opening of the stomata. Light warms the leaf. Light warms the leaf.

TemperatureTemperature

Plants transpire more rapidly at higher Plants transpire more rapidly at higher temperaturestemperatures

Temperature affects humidityTemperature affects humidity

HumidityHumidity

When the surrounding air is dry, diffusion of When the surrounding air is dry, diffusion of water out of the leaf goes on more rapidly. water out of the leaf goes on more rapidly.

Humidity is affected by temperatureHumidity is affected by temperature

WindWind

no breezeno breeze air is humid thus reducing the air is humid thus reducing the rate of transpirationrate of transpiration

breeze breeze humid air is carried away and humid air is carried away and replaced by drier air. replaced by drier air.

Internal Concentration of COInternal Concentration of CO22

If COIf CO2 2 concentration decreases concentration decreases stomata stomata

opensopens

Reverse is also trueReverse is also true

Underlying Principle of the Underlying Principle of the ExperimentExperiment

Rate of transpirationRate of transpiration = Rate of water absorption= Rate of water absorption

Transpiration decreases the water potential in the mesophyll cell, and thus, pulls water up from the roots and stems in vessels and xylems

XylemXylem

Consists of tracheids and vessel cellsConsists of tracheids and vessel cells Transport water Transport water Have pits at the side to allow lateral movementHave pits at the side to allow lateral movement

Plant transpiration

Introduction

Methodology

Summary and Conclusions

Light and Photosynthesis

IntroductionMethodology

Flowchart

Results and discussion

Summary and Conclusions

Results and discussion

Summary and Conclusions

MethodologyMethodology

Control set-upControl set-up Kept inside the room Kept inside the room

120 (botany lab)120 (botany lab) Away from electric Away from electric

fans and windowsfans and windows One branch covered One branch covered

with plasticwith plastic Held by iron clampsHeld by iron clamps Experiment lasts for Experiment lasts for

2 hours2 hours

Under bright light set-upUnder bright light set-up

Kept inside the Kept inside the room 120 (botany room 120 (botany lab)lab)

Away from Away from electric fans but electric fans but BESIDE THE BESIDE THE WINDOWSWINDOWS

Held by iron Held by iron clampsclamps

Experiment lasts Experiment lasts for 2 hoursfor 2 hours

Moving air set-upMoving air set-up

Kept inside the Kept inside the room 120 (botany room 120 (botany lab)lab)

BESIDE the BESIDE the electric fan but electric fan but away from the away from the windowswindows

Held by iron Held by iron clampsclamps

Experiment lasts Experiment lasts for 2 hoursfor 2 hours

Blocked stomata setupBlocked stomata setup

Kept inside the room 120 (botany lab)Kept inside the room 120 (botany lab) Away from the electric fan and from the Away from the electric fan and from the

windowswindows Held by iron clampsHeld by iron clamps Experiment lasts for 2 hoursExperiment lasts for 2 hours Stomata were blocked by Petroleum jellyStomata were blocked by Petroleum jelly

Lateral movement setupLateral movement setup

Lasts for 24 Lasts for 24 hourshours

Plant transpiration

Introduction

Methodology

Summary and Conclusions

Results and discussion

Light and Photosynthesis

IntroductionMethodology

Summary and Conclusions

Results and discussion

Flowchart

Results and DiscussionResults and DiscussionmL of HmL of H22O left O left

unabsorbed unabsorbed

ControlControl 3.23.2

Under strong sunlightUnder strong sunlight 1.51.5

Covered stomataCovered stomata 4.54.5

With Moving airWith Moving air 2.42.4

Versus controlVersus control

Plants absorb more water if exposed to strong Plants absorb more water if exposed to strong light and moving airlight and moving air

More water transpires from the leaves if the More water transpires from the leaves if the plant is exposed to strong sunlight or moving plant is exposed to strong sunlight or moving air air

Versus replicatesVersus replicates

Less water is absorbed if the stomata are Less water is absorbed if the stomata are blockedblocked

Less water is transpired when the stomata are Less water is transpired when the stomata are blockedblocked

Covered branch in ControlCovered branch in Control

Water droplets form at the walls of the plastic Water droplets form at the walls of the plastic

Begonia setupBegonia setup

A tube like structure was observed when begonia A tube like structure was observed when begonia absorbed the dyeabsorbed the dye

Plant transpiration

Introduction

Methodology

Summary and Conclusions

Results and discussion

Light and Photosynthesis

IntroductionMethodology

Summary and Conclusions

Results and discussion

Flowchart

Summary and ConclusionSummary and Conclusion

Plants transpire more if exposed to strong Plants transpire more if exposed to strong light and moving airlight and moving air

Plants transpire less if the stomata are blockedPlants transpire less if the stomata are blocked

ConclusionConclusion

Plants transpire more if the moisture content of air Plants transpire more if the moisture content of air (humidity) is less than the concentration of water (humidity) is less than the concentration of water inside the leavesinside the leaves

Humidity of air adjacent to the plant leaf (or stomata) Humidity of air adjacent to the plant leaf (or stomata) is inversely proportional to heat, light and windis inversely proportional to heat, light and wind

Plants transpire more if there is more heat, light, and Plants transpire more if there is more heat, light, and windwind

Presence of water dropletsPresence of water droplets

Presence of water droplets indicate that water Presence of water droplets indicate that water was released from the branchwas released from the branch

Specifically HSpecifically H220 was released from the stomata 0 was released from the stomata

and accumulated in the walls of the plasticand accumulated in the walls of the plastic

Begonia setupBegonia setup

Water travels through the xylemWater travels through the xylem

Xylary elements are hollow Xylary elements are hollow

Plant transpiration

Introduction

Methodology

Summary and Conclusions

Results and discussion

Light and Photosynthesis

IntroductionMethodology

Summary and Conclusions

Results and discussion

Flowchart

Light and PhotosynthesisLight and Photosynthesis

Plant transpiration

Introduction

Methodology

Summary and Conclusions

Results and discussion

Light and Photosynthesis

IntroductionMethodology

Summary and Conclusions

Results and discussion

Flowchart

PhotosynthesisPhotosynthesis

Uses photons to eject electrons from the trap Uses photons to eject electrons from the trap molecules in photosystems I and IImolecules in photosystems I and II

Thus, the presence of light affects Thus, the presence of light affects photosynthesisphotosynthesis

Plant transpiration

Introduction

Methodology

Summary and Conclusions

Results and discussion

Light and Photosynthesis

IntroductionMethodology

Summary and Conclusions

Results and discussion

Flowchart

MethodologyMethodology

Hydrilla setupHydrilla setup

Bubbles came out from the Bubbles came out from the shoot when the setup was shoot when the setup was exposed to bright lightexposed to bright light

Coleus setupColeus setup

Pigments being Pigments being extracted using 95% extracted using 95% ethanolethanol

Coleus leaf starts to Coleus leaf starts to lose its greenish colorlose its greenish color

Coleus setupColeus setup

IKI dropped to the coleus IKI dropped to the coleus samplesample

IKI is an indicator for the IKI is an indicator for the presence of starchpresence of starch

Coleus sampleColeus sample

Plant transpiration

Introduction

Methodology

Summary and Conclusions

Results and discussion

Light and Photosynthesis

IntroductionMethodology

Summary and Conclusions

Results and discussion

Flowchart

Results and DiscussionsResults and DiscussionsColeus setupColeus setup Bluish coloration on the Bluish coloration on the

uncovered leaf was uncovered leaf was greater compared to that greater compared to that of the covered leafof the covered leaf

Hydrilla setupHydrilla setup Bubbles were released Bubbles were released

upon exposure to upon exposure to sunlightsunlight

Hydrilla setupHydrilla setup

Time intervalTime interval Dim lightDim light Bright lightBright light

1 minutes1 minutes 00 2020

22 00 1717

33 00 1414

44 00 1212

55 00 1212

66 00 1010

77 00 1111

88 00 1414

99 00 1313

1010 00 1212

Hydrilla setupHydrilla setup

1 2 3 4 5 6 7 8 9 10

dim lightbright light

20

17

14

12 12

10 11

1413

12

0 0 0 0 0 0 0 0 0 0

02468

101214161820

minute interval

no. of bubbles per minute

no. of bubbles per minute interval

dim light bright light

Plant transpiration

Introduction

Methodology

Summary and Conclusions

Results and discussion

Light and Photosynthesis

IntroductionMethodology

Summary and Conclusions

Results and discussion

Flowchart

Hydrilla graphHydrilla graph

The number of bubbles seems to be The number of bubbles seems to be diminishing as time passes bydiminishing as time passes by

Dissolved CODissolved CO2 2 may have been used upmay have been used up

No evidence (bubbles) for the occurrence of No evidence (bubbles) for the occurrence of photosynthesis in dim lightphotosynthesis in dim light

Summary and ConclusionSummary and Conclusion

Light is needed in photosynthesisLight is needed in photosynthesis