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Ian A. FlemingDepartment of Ocean Sciences
Memorial University of NewfoundlandSt. John’s, NL
(ifleming@mun.ca)
Role of Habitat Complexity (environmental enrichment) in Salmon Development
Photo by van Ryckevorsel
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Reshaping of Fish
Morphology, physiology, behaviour, life history …
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• But how early does this begin?
Habitat complexity – Early life
Shaping of the phenotype• Morphology• Behaviour• Neural development• Gene expression
Captive - simple
Nature – complex(gravel)
Fitness consequences
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IncubationSimple versus complex (gravel)
• Eggs common pool (85 crosses)• 4,000 eggs per incubation unit
Results – morphology(start of exogenous feeding)
Simple Complex P .
Weight (g) 0.189 < 0.199 < .01*Length (cm) 2.94 ≤ 2.96 = .08Condition -0.012 < 0.016 < .01*(residuals)
Gravel-incubated fish: heavier & higher condition
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Gravel-incubated fish: enhanced feeding & more risk adverse
Results – behaviour(start of exogenous
feeding)
Simple Complex P . Feeding (novel live prey) 74% < 47% = .02*
Simulated PredatorReacted 84% = 90% = .48Sought shelter 59% = 63% = .80 Reemergence (s) 204 < 252 < .01*
Results – brain volume(start of exogenous feeding)
No difference in brain volume
(absolute and size-corrected measures)P .
Whole brain 0.11 - 0.68Telenchepalon 0.68 - 0.73Olfactory bulb 0.76 – 0.83
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Rearing environment affects gene transcription
• degree of response is highly dependent on genetic background
Summary
Incubation environment shapes:• Body morphology (movement, shelter, stress)
• Behaviour (stimulation & complexity)
• Gene expression• BUT not brain volume (cell proliferation,
neuron number, dendrite length )
Do these phenotypic responses translate into performance (growth and survival) in semi-natural
conditions?
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Fitness - Semi-natural streams(start of exogenous feeding)
• 8 replicate streams• 40 simple & 40 complex fish per stream• 42 days
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Results
p = 0.04*
Gro
wth
rate
–le
ngth
(% /
day)
Complex ComplexSimple Simple
Sur
viva
l (#
fish)
48.1% 39.7%
p = 0.03*
Survival Growth
Gravel incubated fish: higher survival & faster growthin semi-natural streams
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0.00%
2.00%
4.00%
6.00%
8.00%
10.00%
12.00%
14.00%
CCC CCS CSC CSS SCC SCS SSC SSS
Overwinter recapture (%)
B B B
P = 0.03
Survival (260 days)
A
A
% re
capt
ured
0
0.1
0.2
0.3
0.4
0.5
0.6
0.7
CCC CCS CSC CSS SCC SCS SSC SSS
Mean SGR adjusted for initial size
C
Specific Growth Rate (260 days)S
peci
fic G
row
th R
ate
(% d
ay-1
)
A AAAB
ABCABC
BC
P < 0.01
Conclusions & Implications
• Incubation environment profound effects on phenotype
• Affects subsequent survival and growth (fitness)
• Density however may be more important than structure as fish grow
• Captive rearing environments can be altered to promote phenotypic traits that may be more favourable in nature
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