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8/13/2019 Osmosis in Red Blood Cell (2ndrevision)
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Osmosis in red blood cell
Group 4
Algenio. Bresnan. Castro. Chang. Tan
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I. Abstract
This study aims to find the effect of osmosisin the cell. The researchers aim to answer thefollowing questions:
1. What are the different effects ofdifferent kinds of solution to the cell?
2. How does osmosis works?
The researchers used red blood cells andNaCl solutions with varying levels of
concentration. The set-ups were observed for anhour and produces different results.
It is later found out that the manipulation ofconcentration levels of the solution affects
osmosis in red blood cells.
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II. Introduction
As the basic unit of life, a cellhas its ownway to self-perpetuate. This may range fromcell division, cellular respiration, up to thetransport of different materials needed forthese processes. One example of such
process is osmosis which occurs inside of thecell. Osmosis is the net movement of a solvent,usually water, through a semi-permeablemembrane.
In this experiment, red blood cells wereused to observe this process of osmosis. Thisis made possible by manipulating theconcentration levels of the solution containing
these RBCs.
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III. Objectives
1. To distinguish the effects of a hypotonic,
hypertonic, and isotonic solution to the cell.
2. To determine how osmosis works.
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IV. Methodology
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1. Extract blood from a volunteer.
Three drops should be placed in
each glass slides labelled A, B,
and C.
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2.Add a drop of 0.07M, 0.15M,
and 0.30M NaCl solution
respectively to each glass
slides.
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3. The red blood cells will beobserved in an hour having five-minute intervals for measuringthe size.
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V. Results and
Discussion
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SAMPLE A0.07 M NaCl Solution
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B & C
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SAMPLE B0.15 M NaCl Solution
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B 0:30
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B. 1:00
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SAMPLE C0.30 M NaCl Solution
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C 0:30
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C 1:00
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Slide A contains a 0.07M NaCl solution.After a 1-hour mark, the cells notably grew
larger.
In slide B, the 0.15M solution does not
cause any changes to the size of the cells.
However in slide C, the 0.30M solution
causes the cells to shrink.
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VI. Conclusion
Since the 0.15 NaCl solution does not cause
any change to the size of the cells,it could be
said that osmosis doesn't takes place.
It can be also concluded that the concentration
inside the red blood cell is somehow near the0.15M margin. This makes the 0.15M NaCl
solution an isotonic one.
With these facts the 0.07M and 0.30M NaClsolutions can be called hypotonic andhypertonic solutions respectively. Thedifference in concentration levels of the cell andthe solution triggers the osmosis process as anattem t of the cell to achieve e uilibrium.
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The molar concentration of the solution isinversely proportional to the size of the red blood
cells. In other words, as you increase the
concentration of the solution, the cells size will
decrease.
Hypertonic solution (high c.) cell shrinks
Isotonic solution no changes in the cell
Hypotonic solution (low c.) cell enlargesand may burst
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