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National Center for Genetic Resources Preservation Fort Collins, CO David Dierig, RL. Research at NCGRP. 6 mm. Non-invasive assays of Seed Longevity and Seed Aging Christina Walters and Dani Ballesteros. - PowerPoint PPT Presentation
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National Center for Genetic Resources Preservation
Fort Collins, CODavid Dierig, RL
•Research at NCGRP
Non-invasive assays of Seed Longevity and Seed Aging
Christina Walters and Dani Ballesteros
Maize inbred (Pioneer) #1, stored 20 yrs -18oC
25oC
Problem: We cannot tell how long a seed will survive during storage, nor how much it has aged, until we germinate it.
• consume seed • are costly• only describe health at the moment
Germination assays
• have no predictive power
Non-invasive assays are quantitative and predictive
• give categorical (not quantitative) information
6 mm
Volatiles production correlates with longevity
Structural stability correlates with longevity
Lipid crystallization decreases with aging
Volatile Analysis using Gas Chromatography
Dynamic Mechanical Analysis
Differential Scanning Calorimetry
Citrus CryopreservationGayle Volk
Concern: Loss of key collection materials due to Huanglongbing diseaseResponse: Develop strategy to cryopreserve vegetative propagules of Citrus accessions• Receive non-dormant bud material from Riverside• Excise 1 mm shoot tips• Cryopreserve using vitrification• Recovery by micrografting onto seedling rootstock in tissue culture
Grafted, cryopreserved Citrus shoot tips 13 days after micrografting
Customer response: Invitations by Citrus Research Board to present at meetings and to submit proposal for industry funding
We developed analytical methods to estimating adaptive differentiation by integrating • Population genetic variation (SNP, SSR)• Ecological data• Spatial niche modeling analysis
How do we efficiently capture genetic diversity in wild populations?
Chris RichardsGermplasm from wild populations is difficult to preserve because it is heterogeneous, not adapted to storage, and difficult to regenerate.
Representative collections, without duplication, require targeting the diversity “hot spots” or genetic lineages.
N. American HopsWith NCGR Corvallis, OR
Little SunflowerWith NCRPIS Ames, IA
How do traits like seed dormancy or after-ripening affect genetic diversity?
Von Mark Cruz and David DierigTraits like after-ripening serve plant populations well in times of extreme environmental conditions to ensure survival but gene flow between plant classes may limit preservation of desired diversity.
Sympatric population of two Lesquerella species in NM
Collections with Gabriella Romano – Parlier, CA
Methods:Phenotypic field measurements and seed collectionsGermination time for plant classes and temperature plus water stress responseMolecular markers to measure diversity Characterization of genes related to seed dormancy
Two plants of the same species at different growth and reproductive maturity stages due to dormancy.
Re-cryoprocessed potato PVP accessions prior to 2004
Maria Jenderek• Objective: Improve quality of potato accessions in
cryopreservation • Problem: Previous protocols lead to low viability after storage
in cryo. • A new protocol was adapted, and 54 accessions are being
reprocessed, thus improving the viability to>50%• Impact: • After PVP expires, the potato cultivars will be integrated into
the public domain (NPGS)• The NCGRP is to be reimbursed $2,400/accession for this
service• The value of the U.S. potato industry is >$3 billion
Personnel IssuesRetired tech position filled in FY10 for
micrografting, tissue culture with Gayle VolkNew Position is planned to coincide with
retirement of Judy Grotenhuis to start in August (Renee White)
New part-time molecular genetics tech Position is being planned with Chris Richards
Post doc started in March with Dave DierigNew HQ post doc to start in Aug with Gayle Volk
Funded SCAs and CRADAs
CSU, CO Seed Lab, $20,000 – Evaluation of seed Longevity of Germplasm Placed in Long Term Storage. Exp. 2010 (plans to renew if possible)
CIMMYT, $38,600 -0 Aid in the Conservation and Regeneration of Maize and Maize Relatives in Mexico. Exp. 2012 (will not fund if no budget increase)
Other Extramural Funds
• New SCA with CSU being developed to collaborate on the Citrus/ Avocado project using HQ funds.
Facility Issues • We have worked with NREL, ClimateWise, and
Lawrence Berkeley Labs on energy audits to reduce utility costs for the facility.
• Roof leaks and age indicate a new roof is needed. We hope to do phase 1 in FY11.
• -18 vault has ice build up issues we’re looking into solutions.
• Phones work on good days.
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