Nanomaterial Biological Imaging and Fate in Zebrafish

Oregon State University

Nanomaterial Biological Imaging and Fate in Zebrafish

Joe FisherTanguay Lab22 Mar 2011

Research: Proof of Concept Stage

Oregon State University

1.5 nm Gold – AF 488 tagged 20x in 5D Zebrafish at 6 dpf

Intestine

Nano Agglomeration

Urogenital

Source: PNNL Storm microscope

Embryo #5

Oregon State University

1.5 nm Gold – AF 488 tagged 20x in 5D Zebrafish at 6 dpf

Intestine

Nano AgglomerationNot yet highlighted in

this animation.

Urogenital

Blood flow in Zebrafish at 30 fps

Note: We could track moving fluorescence.

Source: PNNL Storm microscope

Embryo #2

Oregon State University

Embryo #3

Esophagus ???

Nano Agglomeration

Autofluorescence

Source: PNNL Storm microscope

1.5 nm Gold – AF 488 tagged 20x in 5D Zebrafish at 6 dpf

Oregon State University

Embryo #4

1.5 nm Gold – AF 488 tagged 20x in 5D Zebrafish at 6 dpf

Esophagus ???

Nano Agglomeration

Autofluorescence

Source: PNNL Storm microscope

Oregon State University

Intestine

Nano Agglomeration

Urogenital

Source: PNNL Storm microscope

Embryo #1

1.5 nm Gold – AF 488 tagged 20x in 5D Zebrafish at 6 dpf

Oregon State University

Intestine

Nano Agglomeration

Urogenital

Source: PNNL Storm microscope

Embryo #1

1.5 nm Gold – AF 488 tagged 20x in 5D Zebrafish at 6 dpf

Oregon State University

Intestine

Nano Agglomeration

Swim Bladder

Source: PNNL Storm microscope

Embryo #1

1.5 nm Gold – AF 488 tagged 20x in 5D Zebrafish at 6 dpf

Oregon State University

Nano Agglomeration

Eye

Source: PNNL Storm microscope

Embryo #1

1.5 nm Gold – AF 488 tagged 20x in 5D Zebrafish at 6 dpf