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1
Multifunctional Magnetic
Nanoparticles Bio-application
2
Multifunctional magnetic nanoparticles
components
Magnetic Nanoparticles (MNP)
Gold NPs (GNP)
Nanosilicas (solgels)
Modified Biomolecules- Antibody
- Aptamer
- Single strand DNA
- SiRNA
- hellip
3
4
La Mer theory
and
5
MNP synthetic methods
Coprecipitation
Sol-gel synthesis
Microemulsion synthesis
Sonochemical reaction
Hydrothermal reaction
Thermal decomposition
Electrospray synthesis
Laser pyrolysis
6
7
1- Coprecipitation methods
Morphology Size and Quantity of MNP have been
control by a series of experimental parameters such as
- pH
- Reaction temperature
- Precursor
Disadvantage Expanded size distribution
8
2- New modified Coprecipitation
Ref2 Milichko et al Nanoscale Research Letters 2013 8317 9
3- Modified Hydrothermal reaction
10
4- Thermal decomposition
MNP synthesized by the thermal decomposition of Fe(acac)3 in PEG and in MPEG
Tian XH Wei F Wang TX Wang P Int J Nanomedicine 201271031-4111
5- Sol-gel synthesis
Porous or hollow iron oxide nanostructures have
been synthesized by sol gel process
12
Wrapndash BakendashPeel process
Reduction
MagnetiteSilicaMagnetite
Removal of
silica using
NaOH
β-FeOOH β-FeOOHSilicaHematiteSilica
Removal of
silica using
NaOH
Hematite
Silica
coating
Heat
treatment
13
6- Kirkendall process(Nano hollow structures)
The rate of oxygen diffusion towards the core is slower than
diffusion of iron outwards from the core leading to the formation
of hollow structures
Fe
Fe3O4 Fe3O4
Fe
Fe3O4
Amorphous Crystalline
1 Oxidation by
(CH3)3NO
2 Heating
gt200oC
14
15
Colloidal stability by citrate
Au Au
- ---- - -
- ---O O
OO O
O
O H
Lowis bond
16
Color is size dependent
Size(nm) Emission(nm) Suspension in
5 515-520 PBSsurfactant
10 515-520 PBSsurfactant
15 520 PBSsurfactant
20 524 PBSsurfactant
30 526 PBSsurfactant
40 530 PBSsurfactant
50 535 PBSsurfactant
60 540 PBSsurfactant
80 553 PBSsurfactant
100 572 PBSsurfactant
17
18
GNP Advantage
- Color size dependent
- No toxicity
- DNA strong bonding
- Thiol covalent bond
19
The surface Hydrophobicity can be modified by
thiol compound
20
The surface self-assembling by thiol compound
21
GNP Application
Different bio sensor based self assemble design
22
23
The Sol-Gel Process is
a method for producing solid materials from small molecules
inn RT The method is used for the fabrication of metal oxides
especially the oxides of silicon and titanium
24
Alkoxide-sol-gel-method-preparation
25
HydrolysisCondensation
26
27
Why silica sol-gel coating
- Biocompatible
- Functionalization
- Physical trapping
- Chemical stability
- Physical stability
28
I Byun AW Coleman B Kim Micromech Microeng 23 (2013) 085016 29
Three Dimensional
Multi Dimensional matrix
One Dimensional
Layer by layer coatingSi
OR
ORRORO
Si
OR
RORO
NH2
Si
OR
RORO
SH
Si
OR
RORO O
O
n
Si
OR
RORO
HN
NH
NH2
Si
R
RRORO
30
Two Dimensional
Physical trapping in sol gel
31
32
apto ldquoto fitrdquo
mer ldquosmallest unit of repeating structurerdquo
What is an Aptamer
Oligonucleotide or Peptide
molecules that bind to a specific
target molecule
33
Types of Aptamer
- ssDNA
- dsDNA
- RNA
- Peptide
34
SELEX(Systematic Evolution of Ligands by Exponential Enrichment)
Selection of ligand sequences that bind to a target
partitioning of aptamers from
non-aptamers via affinity methods
amplification of bound aptamers
35
Targets of Aptamers
- Small Molecules
- Proteins
- Nucleic Acids
- Cells
- Tissues
- Organisms
lysozym Thrombin HIV TAR Hemin Interferon γ Vascular
endotherial growth factor (VEGF) Prostate specific antigen(PSA)
Dopamine hellip 36
37
MFMNP Design
Core Shell
Dumbbell Structure
Multicomponent Hybrid NP
38
39
Polymer Shell
Core Shell
Metal alloy
(FePt)
Magnetic nanolayer
(Fe3O4)
Thermal decomposition
of Fe(acac)3 on FePt
Magnetic NP
(Fe3O4)
Metal alloy
(Au Ag)
(Fe3O4) in Au3+ solution
at reduction condition
40
1 Core Shell structure
41
- Mix of NP during the growth of nanocrystals
- Sol-gel process
Core Shell synthesis
2 Dumbbell structures
42
- Mix of NP during the growth of nanocrystals
- Thermal rearrangement
- self-assemble at a liquid-liquid interface
- Functional sol-gel matrix
3 Multicomponent Hybrid NPs
Self-assembly processes provide an approach to
fabricating multicomponent hybrid NPs with
integrated multifunctionality
43
- Surfactant polymers
- Sol-gel process
44
Poly(lactico-co-glycolicacid)
(PLGA)
Poly(L-lysine)-PEG-folate
(PLL-PEG-FOL)
By mechanical
stirring at RT
in CH3Cl
45
46
W Lu SR Arumugam D Senapati ACSNANO 2010 4(3) 1739ndash1749
GNP GNP
GNP
RNA Aptamer
Anti-HER2C-reb-2 antibody
GNPCTAB
47
Magnetic nanoparticle surface modification Glucose oxidasse
(GOx)
PEGGPS
APSMNP
48Z Lin et al Analytica Chimica Acta 818 (2014) 54ndash60
49
P Sun H Zhang C Liu Langmuir 2010 26(2) 1278ndash1284 50
Immuno-labeling using Fe3O4CdTe nanocomposites which are formed by
linking multiple TGA-stabilized CdTe QDs with the thiol-functionalized silica
coated iron oxide nanoparticles
23-dimercaptosuccinic
acids (DMSA)Dopamine
Mercaptoundecanoic acid
(MUA)
HS COOH
4 Small multifunctional molecules
Glutaraldehyde 51
C J Xu K M Xu H W Gu R K Zheng H Liu X X Zhang Z H Guo B Xu J Am Chem Soc 2004 126 9938
Dopamine
Conditions (a) NaOH tert-butyl dicarbonate dioxane H2O 24 hrs (b) BnBr K2CO3 DMF
RT 24 h (c) 10 CF3COOH CH2Cl2 rt 5 h (d) succinic anhydride pyridine RTt 3 h (e)
NHS DCC DMAP CHCl3 RTt 3 h
NTA
52
3-mercapto trimethoxysilane
(MPS)
3-amino trimethoxysilane
(APS)
5 Silane multifunctional monomers
Glycidoxypropyltrimethoxy-silane
(GPS)
53
54
Multifunctional magnetic nanoparticles
application
Bioseparation
Biodetection(Real time monitoring)
Targeted drug delivery
Magnetic fluid Hyperthermia therapy
55
1 Specific Bio-Separation
bull Magnetic properties
bull Targeting agent
Cell organism molecule
56
His-tagged protein purification as
the first step towards recombinant
protein purification
58
Lysing Binding
Nickel
affinity
nanoparicle
His tag
fusion protein
Washing Elusion
Digestion
Binding
Nickel
affinity
nanoparticle
Washing Target
Recombinant
Protein
1- Preparation of Fe3O4SiO2Layered Double Hydroxide CoreminusShell
Microspheres for Magnetic Separation of Proteins J Am ChemSoc (2012)
18 134(2) 1071-7
Ni2+
2- Magnetic Nanocomposite Spheres Decorated with NiO Nanoparticles for a
Magnetically Recyclable Protein Separation System J Kim Y Piao N Lee Y
Park I-H Lee J-H Lee Adv Mater (2010) 22 57ndash60
His-tagged protein purification
Purification of His6-lipase using Fe3O4AundashLAndashIDAndashCu2+ NPs with various
amounts of NPs (lane 1 01 mg lane 2 02 mg lane 3
63
Synthetic procedure for the coreshell structure spheres
64
glutaraldehyde
(GLH)
Reference[1] H W Gu Z M Yang J H Gao C K Chang B Xu J Am Chem Soc 2005 127 34
[2] A Hutten D Sudfeld I Ennen G Reiss K Wojczykowski P Jutzi J Magn Magn Mater 2005
293 93
[3] G S Chaubey C Barcena N Poudyal C B Rong J M Gao S Sun J P Liu J Am Chem
Soc 2007 129 7214
[4] C W Kim Y H Kim H G Cha D K Lee Y S Kang J NanosciNanotechnol 2006 6 3417
[5] Y W Jun J S Choi J Cheon Angew Chem Int Ed 2006 45 3414
[6] X Michalet F F Pinaud L A Bentolila J M Tsay S Doose J J Li G Sundaresan A M Wu
S S Gambhir S Weiss Science 2005 307 538
[7] M Hu J Y Chen Z Y Li L Au G V Hartland X D Li M Marquez Y N Xia Chem Soc
Rev 2006 35 1084
[8] M Vallet-Regi F Balas D Arcos Angew Chem Int Ed 2007 46 7548
[9] S T Selvan P K Patra C Y Ang J Y Ying Angew Chem Int Ed 2007 46 2448
[10] I S Lee N Lee J Park B H Kim Y W Yi T Kim T K Kim I H Lee S R Paik T
Hyeon J Am Chem Soc 2006 128 10658
[11] Y S Lin S H Wu Y Hung Y H Chou C Chang M L Lin C P Tsai C Y Mou Chem
Mater 2006 18 5170
[12] B H Wu H Zhang C Chen S C Lin N F Zheng Nano Res 2009 2 975
[13] J Kim J E Lee J Lee J H Yu B C Kim K An Y Hwang C Shin J G Park J Kim T
Hyeon J Am Chem Soc 2006 128 688
[14] S Santra H S Yang P H Holloway J T Stanley R A Mericle J Am Chem Soc 2005 127
1656
[15] D K Yi S T Selvan S S Lee G C Papaefthymiou D Kundaliya J Y Ying J Am Chem
Soc 2005 127 4990 65
[16] J H Gao B Zhang Y Gao Y Pan X X Zhang B Xu J Am Chem Soc 2007 129 11928 [17] J H Gao W Zhang P B Huang B Zhang X X Zhang B Xu J Am Chem Soc 2008 130 3710 [18] J H Gao G L Liang B Zhang Y Kuang X X Zhang B Xu J Am Chem Soc 2007 129 1428 [19] J H Gao G L Liang J S Cheung Y Pan Y Kuang F Zhao B Zhang X X Zhang E X Wu B Xu J Am Chem Soc 2008 130 11828 [20] A R Kortan R Hull R L Opila M G Bawendi M L Steigerwald P J Carroll L E Brus JAm Chem Soc 1990 112 1327 [21] X G Peng M C Schlamp A V Kadavanich A P Alivisatos J Am Chem Soc 1997 119 7019 [22] S Kim B Fisher H J Eisler M Bawendi J Am Chem Soc 2003 125 11466[23] H Zeng J Li Z L Wang J P Liu S Sun Nano Lett 2004 4 187 [24] H Zeng S Sun J Li Z L Wang J P Liu Appl Phys Lett 2004 85 792 [25] Z Xu Y Hou S Sun J Am Chem Soc 2007 129 8698 [26] Y H Lin N Kim J Q Wang C J Zhong J Phys Chem B 2005 109 21593 [27] C Wang H Daimon S Sun Nano Lett 2009 9 1493 [28] K W Kwon M Shim J Am Chem Soc 2005 127 10269 [29] H Yu M Chen P M Rice S X Wang R L White S Sun Nano Lett 2005 5 379 [30] J S Choi Y W Jun S I Yeon H C Kim J S Shin J Cheon J Am Chem Soc 2006 128 15982 [31] S H Choi H B Na Y I Park K An S G Kwon Y Jang M Park J Moon J S Son I CSong W K Moon T Hyeon J Am Chem Soc 2008 130 15573 [32] H W Gu R K Zheng X X Zhang B Xu J Am Chem Soc 2004 126 5664
66
Multifunctional Magnetic
Nanoparticles Bio-application
2
Multifunctional magnetic nanoparticles
components
Magnetic Nanoparticles (MNP)
Gold NPs (GNP)
Nanosilicas (solgels)
Modified Biomolecules- Antibody
- Aptamer
- Single strand DNA
- SiRNA
- hellip
3
4
La Mer theory
and
5
MNP synthetic methods
Coprecipitation
Sol-gel synthesis
Microemulsion synthesis
Sonochemical reaction
Hydrothermal reaction
Thermal decomposition
Electrospray synthesis
Laser pyrolysis
6
7
1- Coprecipitation methods
Morphology Size and Quantity of MNP have been
control by a series of experimental parameters such as
- pH
- Reaction temperature
- Precursor
Disadvantage Expanded size distribution
8
2- New modified Coprecipitation
Ref2 Milichko et al Nanoscale Research Letters 2013 8317 9
3- Modified Hydrothermal reaction
10
4- Thermal decomposition
MNP synthesized by the thermal decomposition of Fe(acac)3 in PEG and in MPEG
Tian XH Wei F Wang TX Wang P Int J Nanomedicine 201271031-4111
5- Sol-gel synthesis
Porous or hollow iron oxide nanostructures have
been synthesized by sol gel process
12
Wrapndash BakendashPeel process
Reduction
MagnetiteSilicaMagnetite
Removal of
silica using
NaOH
β-FeOOH β-FeOOHSilicaHematiteSilica
Removal of
silica using
NaOH
Hematite
Silica
coating
Heat
treatment
13
6- Kirkendall process(Nano hollow structures)
The rate of oxygen diffusion towards the core is slower than
diffusion of iron outwards from the core leading to the formation
of hollow structures
Fe
Fe3O4 Fe3O4
Fe
Fe3O4
Amorphous Crystalline
1 Oxidation by
(CH3)3NO
2 Heating
gt200oC
14
15
Colloidal stability by citrate
Au Au
- ---- - -
- ---O O
OO O
O
O H
Lowis bond
16
Color is size dependent
Size(nm) Emission(nm) Suspension in
5 515-520 PBSsurfactant
10 515-520 PBSsurfactant
15 520 PBSsurfactant
20 524 PBSsurfactant
30 526 PBSsurfactant
40 530 PBSsurfactant
50 535 PBSsurfactant
60 540 PBSsurfactant
80 553 PBSsurfactant
100 572 PBSsurfactant
17
18
GNP Advantage
- Color size dependent
- No toxicity
- DNA strong bonding
- Thiol covalent bond
19
The surface Hydrophobicity can be modified by
thiol compound
20
The surface self-assembling by thiol compound
21
GNP Application
Different bio sensor based self assemble design
22
23
The Sol-Gel Process is
a method for producing solid materials from small molecules
inn RT The method is used for the fabrication of metal oxides
especially the oxides of silicon and titanium
24
Alkoxide-sol-gel-method-preparation
25
HydrolysisCondensation
26
27
Why silica sol-gel coating
- Biocompatible
- Functionalization
- Physical trapping
- Chemical stability
- Physical stability
28
I Byun AW Coleman B Kim Micromech Microeng 23 (2013) 085016 29
Three Dimensional
Multi Dimensional matrix
One Dimensional
Layer by layer coatingSi
OR
ORRORO
Si
OR
RORO
NH2
Si
OR
RORO
SH
Si
OR
RORO O
O
n
Si
OR
RORO
HN
NH
NH2
Si
R
RRORO
30
Two Dimensional
Physical trapping in sol gel
31
32
apto ldquoto fitrdquo
mer ldquosmallest unit of repeating structurerdquo
What is an Aptamer
Oligonucleotide or Peptide
molecules that bind to a specific
target molecule
33
Types of Aptamer
- ssDNA
- dsDNA
- RNA
- Peptide
34
SELEX(Systematic Evolution of Ligands by Exponential Enrichment)
Selection of ligand sequences that bind to a target
partitioning of aptamers from
non-aptamers via affinity methods
amplification of bound aptamers
35
Targets of Aptamers
- Small Molecules
- Proteins
- Nucleic Acids
- Cells
- Tissues
- Organisms
lysozym Thrombin HIV TAR Hemin Interferon γ Vascular
endotherial growth factor (VEGF) Prostate specific antigen(PSA)
Dopamine hellip 36
37
MFMNP Design
Core Shell
Dumbbell Structure
Multicomponent Hybrid NP
38
39
Polymer Shell
Core Shell
Metal alloy
(FePt)
Magnetic nanolayer
(Fe3O4)
Thermal decomposition
of Fe(acac)3 on FePt
Magnetic NP
(Fe3O4)
Metal alloy
(Au Ag)
(Fe3O4) in Au3+ solution
at reduction condition
40
1 Core Shell structure
41
- Mix of NP during the growth of nanocrystals
- Sol-gel process
Core Shell synthesis
2 Dumbbell structures
42
- Mix of NP during the growth of nanocrystals
- Thermal rearrangement
- self-assemble at a liquid-liquid interface
- Functional sol-gel matrix
3 Multicomponent Hybrid NPs
Self-assembly processes provide an approach to
fabricating multicomponent hybrid NPs with
integrated multifunctionality
43
- Surfactant polymers
- Sol-gel process
44
Poly(lactico-co-glycolicacid)
(PLGA)
Poly(L-lysine)-PEG-folate
(PLL-PEG-FOL)
By mechanical
stirring at RT
in CH3Cl
45
46
W Lu SR Arumugam D Senapati ACSNANO 2010 4(3) 1739ndash1749
GNP GNP
GNP
RNA Aptamer
Anti-HER2C-reb-2 antibody
GNPCTAB
47
Magnetic nanoparticle surface modification Glucose oxidasse
(GOx)
PEGGPS
APSMNP
48Z Lin et al Analytica Chimica Acta 818 (2014) 54ndash60
49
P Sun H Zhang C Liu Langmuir 2010 26(2) 1278ndash1284 50
Immuno-labeling using Fe3O4CdTe nanocomposites which are formed by
linking multiple TGA-stabilized CdTe QDs with the thiol-functionalized silica
coated iron oxide nanoparticles
23-dimercaptosuccinic
acids (DMSA)Dopamine
Mercaptoundecanoic acid
(MUA)
HS COOH
4 Small multifunctional molecules
Glutaraldehyde 51
C J Xu K M Xu H W Gu R K Zheng H Liu X X Zhang Z H Guo B Xu J Am Chem Soc 2004 126 9938
Dopamine
Conditions (a) NaOH tert-butyl dicarbonate dioxane H2O 24 hrs (b) BnBr K2CO3 DMF
RT 24 h (c) 10 CF3COOH CH2Cl2 rt 5 h (d) succinic anhydride pyridine RTt 3 h (e)
NHS DCC DMAP CHCl3 RTt 3 h
NTA
52
3-mercapto trimethoxysilane
(MPS)
3-amino trimethoxysilane
(APS)
5 Silane multifunctional monomers
Glycidoxypropyltrimethoxy-silane
(GPS)
53
54
Multifunctional magnetic nanoparticles
application
Bioseparation
Biodetection(Real time monitoring)
Targeted drug delivery
Magnetic fluid Hyperthermia therapy
55
1 Specific Bio-Separation
bull Magnetic properties
bull Targeting agent
Cell organism molecule
56
His-tagged protein purification as
the first step towards recombinant
protein purification
58
Lysing Binding
Nickel
affinity
nanoparicle
His tag
fusion protein
Washing Elusion
Digestion
Binding
Nickel
affinity
nanoparticle
Washing Target
Recombinant
Protein
1- Preparation of Fe3O4SiO2Layered Double Hydroxide CoreminusShell
Microspheres for Magnetic Separation of Proteins J Am ChemSoc (2012)
18 134(2) 1071-7
Ni2+
2- Magnetic Nanocomposite Spheres Decorated with NiO Nanoparticles for a
Magnetically Recyclable Protein Separation System J Kim Y Piao N Lee Y
Park I-H Lee J-H Lee Adv Mater (2010) 22 57ndash60
His-tagged protein purification
Purification of His6-lipase using Fe3O4AundashLAndashIDAndashCu2+ NPs with various
amounts of NPs (lane 1 01 mg lane 2 02 mg lane 3
63
Synthetic procedure for the coreshell structure spheres
64
glutaraldehyde
(GLH)
Reference[1] H W Gu Z M Yang J H Gao C K Chang B Xu J Am Chem Soc 2005 127 34
[2] A Hutten D Sudfeld I Ennen G Reiss K Wojczykowski P Jutzi J Magn Magn Mater 2005
293 93
[3] G S Chaubey C Barcena N Poudyal C B Rong J M Gao S Sun J P Liu J Am Chem
Soc 2007 129 7214
[4] C W Kim Y H Kim H G Cha D K Lee Y S Kang J NanosciNanotechnol 2006 6 3417
[5] Y W Jun J S Choi J Cheon Angew Chem Int Ed 2006 45 3414
[6] X Michalet F F Pinaud L A Bentolila J M Tsay S Doose J J Li G Sundaresan A M Wu
S S Gambhir S Weiss Science 2005 307 538
[7] M Hu J Y Chen Z Y Li L Au G V Hartland X D Li M Marquez Y N Xia Chem Soc
Rev 2006 35 1084
[8] M Vallet-Regi F Balas D Arcos Angew Chem Int Ed 2007 46 7548
[9] S T Selvan P K Patra C Y Ang J Y Ying Angew Chem Int Ed 2007 46 2448
[10] I S Lee N Lee J Park B H Kim Y W Yi T Kim T K Kim I H Lee S R Paik T
Hyeon J Am Chem Soc 2006 128 10658
[11] Y S Lin S H Wu Y Hung Y H Chou C Chang M L Lin C P Tsai C Y Mou Chem
Mater 2006 18 5170
[12] B H Wu H Zhang C Chen S C Lin N F Zheng Nano Res 2009 2 975
[13] J Kim J E Lee J Lee J H Yu B C Kim K An Y Hwang C Shin J G Park J Kim T
Hyeon J Am Chem Soc 2006 128 688
[14] S Santra H S Yang P H Holloway J T Stanley R A Mericle J Am Chem Soc 2005 127
1656
[15] D K Yi S T Selvan S S Lee G C Papaefthymiou D Kundaliya J Y Ying J Am Chem
Soc 2005 127 4990 65
[16] J H Gao B Zhang Y Gao Y Pan X X Zhang B Xu J Am Chem Soc 2007 129 11928 [17] J H Gao W Zhang P B Huang B Zhang X X Zhang B Xu J Am Chem Soc 2008 130 3710 [18] J H Gao G L Liang B Zhang Y Kuang X X Zhang B Xu J Am Chem Soc 2007 129 1428 [19] J H Gao G L Liang J S Cheung Y Pan Y Kuang F Zhao B Zhang X X Zhang E X Wu B Xu J Am Chem Soc 2008 130 11828 [20] A R Kortan R Hull R L Opila M G Bawendi M L Steigerwald P J Carroll L E Brus JAm Chem Soc 1990 112 1327 [21] X G Peng M C Schlamp A V Kadavanich A P Alivisatos J Am Chem Soc 1997 119 7019 [22] S Kim B Fisher H J Eisler M Bawendi J Am Chem Soc 2003 125 11466[23] H Zeng J Li Z L Wang J P Liu S Sun Nano Lett 2004 4 187 [24] H Zeng S Sun J Li Z L Wang J P Liu Appl Phys Lett 2004 85 792 [25] Z Xu Y Hou S Sun J Am Chem Soc 2007 129 8698 [26] Y H Lin N Kim J Q Wang C J Zhong J Phys Chem B 2005 109 21593 [27] C Wang H Daimon S Sun Nano Lett 2009 9 1493 [28] K W Kwon M Shim J Am Chem Soc 2005 127 10269 [29] H Yu M Chen P M Rice S X Wang R L White S Sun Nano Lett 2005 5 379 [30] J S Choi Y W Jun S I Yeon H C Kim J S Shin J Cheon J Am Chem Soc 2006 128 15982 [31] S H Choi H B Na Y I Park K An S G Kwon Y Jang M Park J Moon J S Son I CSong W K Moon T Hyeon J Am Chem Soc 2008 130 15573 [32] H W Gu R K Zheng X X Zhang B Xu J Am Chem Soc 2004 126 5664
66
Multifunctional magnetic nanoparticles
components
Magnetic Nanoparticles (MNP)
Gold NPs (GNP)
Nanosilicas (solgels)
Modified Biomolecules- Antibody
- Aptamer
- Single strand DNA
- SiRNA
- hellip
3
4
La Mer theory
and
5
MNP synthetic methods
Coprecipitation
Sol-gel synthesis
Microemulsion synthesis
Sonochemical reaction
Hydrothermal reaction
Thermal decomposition
Electrospray synthesis
Laser pyrolysis
6
7
1- Coprecipitation methods
Morphology Size and Quantity of MNP have been
control by a series of experimental parameters such as
- pH
- Reaction temperature
- Precursor
Disadvantage Expanded size distribution
8
2- New modified Coprecipitation
Ref2 Milichko et al Nanoscale Research Letters 2013 8317 9
3- Modified Hydrothermal reaction
10
4- Thermal decomposition
MNP synthesized by the thermal decomposition of Fe(acac)3 in PEG and in MPEG
Tian XH Wei F Wang TX Wang P Int J Nanomedicine 201271031-4111
5- Sol-gel synthesis
Porous or hollow iron oxide nanostructures have
been synthesized by sol gel process
12
Wrapndash BakendashPeel process
Reduction
MagnetiteSilicaMagnetite
Removal of
silica using
NaOH
β-FeOOH β-FeOOHSilicaHematiteSilica
Removal of
silica using
NaOH
Hematite
Silica
coating
Heat
treatment
13
6- Kirkendall process(Nano hollow structures)
The rate of oxygen diffusion towards the core is slower than
diffusion of iron outwards from the core leading to the formation
of hollow structures
Fe
Fe3O4 Fe3O4
Fe
Fe3O4
Amorphous Crystalline
1 Oxidation by
(CH3)3NO
2 Heating
gt200oC
14
15
Colloidal stability by citrate
Au Au
- ---- - -
- ---O O
OO O
O
O H
Lowis bond
16
Color is size dependent
Size(nm) Emission(nm) Suspension in
5 515-520 PBSsurfactant
10 515-520 PBSsurfactant
15 520 PBSsurfactant
20 524 PBSsurfactant
30 526 PBSsurfactant
40 530 PBSsurfactant
50 535 PBSsurfactant
60 540 PBSsurfactant
80 553 PBSsurfactant
100 572 PBSsurfactant
17
18
GNP Advantage
- Color size dependent
- No toxicity
- DNA strong bonding
- Thiol covalent bond
19
The surface Hydrophobicity can be modified by
thiol compound
20
The surface self-assembling by thiol compound
21
GNP Application
Different bio sensor based self assemble design
22
23
The Sol-Gel Process is
a method for producing solid materials from small molecules
inn RT The method is used for the fabrication of metal oxides
especially the oxides of silicon and titanium
24
Alkoxide-sol-gel-method-preparation
25
HydrolysisCondensation
26
27
Why silica sol-gel coating
- Biocompatible
- Functionalization
- Physical trapping
- Chemical stability
- Physical stability
28
I Byun AW Coleman B Kim Micromech Microeng 23 (2013) 085016 29
Three Dimensional
Multi Dimensional matrix
One Dimensional
Layer by layer coatingSi
OR
ORRORO
Si
OR
RORO
NH2
Si
OR
RORO
SH
Si
OR
RORO O
O
n
Si
OR
RORO
HN
NH
NH2
Si
R
RRORO
30
Two Dimensional
Physical trapping in sol gel
31
32
apto ldquoto fitrdquo
mer ldquosmallest unit of repeating structurerdquo
What is an Aptamer
Oligonucleotide or Peptide
molecules that bind to a specific
target molecule
33
Types of Aptamer
- ssDNA
- dsDNA
- RNA
- Peptide
34
SELEX(Systematic Evolution of Ligands by Exponential Enrichment)
Selection of ligand sequences that bind to a target
partitioning of aptamers from
non-aptamers via affinity methods
amplification of bound aptamers
35
Targets of Aptamers
- Small Molecules
- Proteins
- Nucleic Acids
- Cells
- Tissues
- Organisms
lysozym Thrombin HIV TAR Hemin Interferon γ Vascular
endotherial growth factor (VEGF) Prostate specific antigen(PSA)
Dopamine hellip 36
37
MFMNP Design
Core Shell
Dumbbell Structure
Multicomponent Hybrid NP
38
39
Polymer Shell
Core Shell
Metal alloy
(FePt)
Magnetic nanolayer
(Fe3O4)
Thermal decomposition
of Fe(acac)3 on FePt
Magnetic NP
(Fe3O4)
Metal alloy
(Au Ag)
(Fe3O4) in Au3+ solution
at reduction condition
40
1 Core Shell structure
41
- Mix of NP during the growth of nanocrystals
- Sol-gel process
Core Shell synthesis
2 Dumbbell structures
42
- Mix of NP during the growth of nanocrystals
- Thermal rearrangement
- self-assemble at a liquid-liquid interface
- Functional sol-gel matrix
3 Multicomponent Hybrid NPs
Self-assembly processes provide an approach to
fabricating multicomponent hybrid NPs with
integrated multifunctionality
43
- Surfactant polymers
- Sol-gel process
44
Poly(lactico-co-glycolicacid)
(PLGA)
Poly(L-lysine)-PEG-folate
(PLL-PEG-FOL)
By mechanical
stirring at RT
in CH3Cl
45
46
W Lu SR Arumugam D Senapati ACSNANO 2010 4(3) 1739ndash1749
GNP GNP
GNP
RNA Aptamer
Anti-HER2C-reb-2 antibody
GNPCTAB
47
Magnetic nanoparticle surface modification Glucose oxidasse
(GOx)
PEGGPS
APSMNP
48Z Lin et al Analytica Chimica Acta 818 (2014) 54ndash60
49
P Sun H Zhang C Liu Langmuir 2010 26(2) 1278ndash1284 50
Immuno-labeling using Fe3O4CdTe nanocomposites which are formed by
linking multiple TGA-stabilized CdTe QDs with the thiol-functionalized silica
coated iron oxide nanoparticles
23-dimercaptosuccinic
acids (DMSA)Dopamine
Mercaptoundecanoic acid
(MUA)
HS COOH
4 Small multifunctional molecules
Glutaraldehyde 51
C J Xu K M Xu H W Gu R K Zheng H Liu X X Zhang Z H Guo B Xu J Am Chem Soc 2004 126 9938
Dopamine
Conditions (a) NaOH tert-butyl dicarbonate dioxane H2O 24 hrs (b) BnBr K2CO3 DMF
RT 24 h (c) 10 CF3COOH CH2Cl2 rt 5 h (d) succinic anhydride pyridine RTt 3 h (e)
NHS DCC DMAP CHCl3 RTt 3 h
NTA
52
3-mercapto trimethoxysilane
(MPS)
3-amino trimethoxysilane
(APS)
5 Silane multifunctional monomers
Glycidoxypropyltrimethoxy-silane
(GPS)
53
54
Multifunctional magnetic nanoparticles
application
Bioseparation
Biodetection(Real time monitoring)
Targeted drug delivery
Magnetic fluid Hyperthermia therapy
55
1 Specific Bio-Separation
bull Magnetic properties
bull Targeting agent
Cell organism molecule
56
His-tagged protein purification as
the first step towards recombinant
protein purification
58
Lysing Binding
Nickel
affinity
nanoparicle
His tag
fusion protein
Washing Elusion
Digestion
Binding
Nickel
affinity
nanoparticle
Washing Target
Recombinant
Protein
1- Preparation of Fe3O4SiO2Layered Double Hydroxide CoreminusShell
Microspheres for Magnetic Separation of Proteins J Am ChemSoc (2012)
18 134(2) 1071-7
Ni2+
2- Magnetic Nanocomposite Spheres Decorated with NiO Nanoparticles for a
Magnetically Recyclable Protein Separation System J Kim Y Piao N Lee Y
Park I-H Lee J-H Lee Adv Mater (2010) 22 57ndash60
His-tagged protein purification
Purification of His6-lipase using Fe3O4AundashLAndashIDAndashCu2+ NPs with various
amounts of NPs (lane 1 01 mg lane 2 02 mg lane 3
63
Synthetic procedure for the coreshell structure spheres
64
glutaraldehyde
(GLH)
Reference[1] H W Gu Z M Yang J H Gao C K Chang B Xu J Am Chem Soc 2005 127 34
[2] A Hutten D Sudfeld I Ennen G Reiss K Wojczykowski P Jutzi J Magn Magn Mater 2005
293 93
[3] G S Chaubey C Barcena N Poudyal C B Rong J M Gao S Sun J P Liu J Am Chem
Soc 2007 129 7214
[4] C W Kim Y H Kim H G Cha D K Lee Y S Kang J NanosciNanotechnol 2006 6 3417
[5] Y W Jun J S Choi J Cheon Angew Chem Int Ed 2006 45 3414
[6] X Michalet F F Pinaud L A Bentolila J M Tsay S Doose J J Li G Sundaresan A M Wu
S S Gambhir S Weiss Science 2005 307 538
[7] M Hu J Y Chen Z Y Li L Au G V Hartland X D Li M Marquez Y N Xia Chem Soc
Rev 2006 35 1084
[8] M Vallet-Regi F Balas D Arcos Angew Chem Int Ed 2007 46 7548
[9] S T Selvan P K Patra C Y Ang J Y Ying Angew Chem Int Ed 2007 46 2448
[10] I S Lee N Lee J Park B H Kim Y W Yi T Kim T K Kim I H Lee S R Paik T
Hyeon J Am Chem Soc 2006 128 10658
[11] Y S Lin S H Wu Y Hung Y H Chou C Chang M L Lin C P Tsai C Y Mou Chem
Mater 2006 18 5170
[12] B H Wu H Zhang C Chen S C Lin N F Zheng Nano Res 2009 2 975
[13] J Kim J E Lee J Lee J H Yu B C Kim K An Y Hwang C Shin J G Park J Kim T
Hyeon J Am Chem Soc 2006 128 688
[14] S Santra H S Yang P H Holloway J T Stanley R A Mericle J Am Chem Soc 2005 127
1656
[15] D K Yi S T Selvan S S Lee G C Papaefthymiou D Kundaliya J Y Ying J Am Chem
Soc 2005 127 4990 65
[16] J H Gao B Zhang Y Gao Y Pan X X Zhang B Xu J Am Chem Soc 2007 129 11928 [17] J H Gao W Zhang P B Huang B Zhang X X Zhang B Xu J Am Chem Soc 2008 130 3710 [18] J H Gao G L Liang B Zhang Y Kuang X X Zhang B Xu J Am Chem Soc 2007 129 1428 [19] J H Gao G L Liang J S Cheung Y Pan Y Kuang F Zhao B Zhang X X Zhang E X Wu B Xu J Am Chem Soc 2008 130 11828 [20] A R Kortan R Hull R L Opila M G Bawendi M L Steigerwald P J Carroll L E Brus JAm Chem Soc 1990 112 1327 [21] X G Peng M C Schlamp A V Kadavanich A P Alivisatos J Am Chem Soc 1997 119 7019 [22] S Kim B Fisher H J Eisler M Bawendi J Am Chem Soc 2003 125 11466[23] H Zeng J Li Z L Wang J P Liu S Sun Nano Lett 2004 4 187 [24] H Zeng S Sun J Li Z L Wang J P Liu Appl Phys Lett 2004 85 792 [25] Z Xu Y Hou S Sun J Am Chem Soc 2007 129 8698 [26] Y H Lin N Kim J Q Wang C J Zhong J Phys Chem B 2005 109 21593 [27] C Wang H Daimon S Sun Nano Lett 2009 9 1493 [28] K W Kwon M Shim J Am Chem Soc 2005 127 10269 [29] H Yu M Chen P M Rice S X Wang R L White S Sun Nano Lett 2005 5 379 [30] J S Choi Y W Jun S I Yeon H C Kim J S Shin J Cheon J Am Chem Soc 2006 128 15982 [31] S H Choi H B Na Y I Park K An S G Kwon Y Jang M Park J Moon J S Son I CSong W K Moon T Hyeon J Am Chem Soc 2008 130 15573 [32] H W Gu R K Zheng X X Zhang B Xu J Am Chem Soc 2004 126 5664
66
4
La Mer theory
and
5
MNP synthetic methods
Coprecipitation
Sol-gel synthesis
Microemulsion synthesis
Sonochemical reaction
Hydrothermal reaction
Thermal decomposition
Electrospray synthesis
Laser pyrolysis
6
7
1- Coprecipitation methods
Morphology Size and Quantity of MNP have been
control by a series of experimental parameters such as
- pH
- Reaction temperature
- Precursor
Disadvantage Expanded size distribution
8
2- New modified Coprecipitation
Ref2 Milichko et al Nanoscale Research Letters 2013 8317 9
3- Modified Hydrothermal reaction
10
4- Thermal decomposition
MNP synthesized by the thermal decomposition of Fe(acac)3 in PEG and in MPEG
Tian XH Wei F Wang TX Wang P Int J Nanomedicine 201271031-4111
5- Sol-gel synthesis
Porous or hollow iron oxide nanostructures have
been synthesized by sol gel process
12
Wrapndash BakendashPeel process
Reduction
MagnetiteSilicaMagnetite
Removal of
silica using
NaOH
β-FeOOH β-FeOOHSilicaHematiteSilica
Removal of
silica using
NaOH
Hematite
Silica
coating
Heat
treatment
13
6- Kirkendall process(Nano hollow structures)
The rate of oxygen diffusion towards the core is slower than
diffusion of iron outwards from the core leading to the formation
of hollow structures
Fe
Fe3O4 Fe3O4
Fe
Fe3O4
Amorphous Crystalline
1 Oxidation by
(CH3)3NO
2 Heating
gt200oC
14
15
Colloidal stability by citrate
Au Au
- ---- - -
- ---O O
OO O
O
O H
Lowis bond
16
Color is size dependent
Size(nm) Emission(nm) Suspension in
5 515-520 PBSsurfactant
10 515-520 PBSsurfactant
15 520 PBSsurfactant
20 524 PBSsurfactant
30 526 PBSsurfactant
40 530 PBSsurfactant
50 535 PBSsurfactant
60 540 PBSsurfactant
80 553 PBSsurfactant
100 572 PBSsurfactant
17
18
GNP Advantage
- Color size dependent
- No toxicity
- DNA strong bonding
- Thiol covalent bond
19
The surface Hydrophobicity can be modified by
thiol compound
20
The surface self-assembling by thiol compound
21
GNP Application
Different bio sensor based self assemble design
22
23
The Sol-Gel Process is
a method for producing solid materials from small molecules
inn RT The method is used for the fabrication of metal oxides
especially the oxides of silicon and titanium
24
Alkoxide-sol-gel-method-preparation
25
HydrolysisCondensation
26
27
Why silica sol-gel coating
- Biocompatible
- Functionalization
- Physical trapping
- Chemical stability
- Physical stability
28
I Byun AW Coleman B Kim Micromech Microeng 23 (2013) 085016 29
Three Dimensional
Multi Dimensional matrix
One Dimensional
Layer by layer coatingSi
OR
ORRORO
Si
OR
RORO
NH2
Si
OR
RORO
SH
Si
OR
RORO O
O
n
Si
OR
RORO
HN
NH
NH2
Si
R
RRORO
30
Two Dimensional
Physical trapping in sol gel
31
32
apto ldquoto fitrdquo
mer ldquosmallest unit of repeating structurerdquo
What is an Aptamer
Oligonucleotide or Peptide
molecules that bind to a specific
target molecule
33
Types of Aptamer
- ssDNA
- dsDNA
- RNA
- Peptide
34
SELEX(Systematic Evolution of Ligands by Exponential Enrichment)
Selection of ligand sequences that bind to a target
partitioning of aptamers from
non-aptamers via affinity methods
amplification of bound aptamers
35
Targets of Aptamers
- Small Molecules
- Proteins
- Nucleic Acids
- Cells
- Tissues
- Organisms
lysozym Thrombin HIV TAR Hemin Interferon γ Vascular
endotherial growth factor (VEGF) Prostate specific antigen(PSA)
Dopamine hellip 36
37
MFMNP Design
Core Shell
Dumbbell Structure
Multicomponent Hybrid NP
38
39
Polymer Shell
Core Shell
Metal alloy
(FePt)
Magnetic nanolayer
(Fe3O4)
Thermal decomposition
of Fe(acac)3 on FePt
Magnetic NP
(Fe3O4)
Metal alloy
(Au Ag)
(Fe3O4) in Au3+ solution
at reduction condition
40
1 Core Shell structure
41
- Mix of NP during the growth of nanocrystals
- Sol-gel process
Core Shell synthesis
2 Dumbbell structures
42
- Mix of NP during the growth of nanocrystals
- Thermal rearrangement
- self-assemble at a liquid-liquid interface
- Functional sol-gel matrix
3 Multicomponent Hybrid NPs
Self-assembly processes provide an approach to
fabricating multicomponent hybrid NPs with
integrated multifunctionality
43
- Surfactant polymers
- Sol-gel process
44
Poly(lactico-co-glycolicacid)
(PLGA)
Poly(L-lysine)-PEG-folate
(PLL-PEG-FOL)
By mechanical
stirring at RT
in CH3Cl
45
46
W Lu SR Arumugam D Senapati ACSNANO 2010 4(3) 1739ndash1749
GNP GNP
GNP
RNA Aptamer
Anti-HER2C-reb-2 antibody
GNPCTAB
47
Magnetic nanoparticle surface modification Glucose oxidasse
(GOx)
PEGGPS
APSMNP
48Z Lin et al Analytica Chimica Acta 818 (2014) 54ndash60
49
P Sun H Zhang C Liu Langmuir 2010 26(2) 1278ndash1284 50
Immuno-labeling using Fe3O4CdTe nanocomposites which are formed by
linking multiple TGA-stabilized CdTe QDs with the thiol-functionalized silica
coated iron oxide nanoparticles
23-dimercaptosuccinic
acids (DMSA)Dopamine
Mercaptoundecanoic acid
(MUA)
HS COOH
4 Small multifunctional molecules
Glutaraldehyde 51
C J Xu K M Xu H W Gu R K Zheng H Liu X X Zhang Z H Guo B Xu J Am Chem Soc 2004 126 9938
Dopamine
Conditions (a) NaOH tert-butyl dicarbonate dioxane H2O 24 hrs (b) BnBr K2CO3 DMF
RT 24 h (c) 10 CF3COOH CH2Cl2 rt 5 h (d) succinic anhydride pyridine RTt 3 h (e)
NHS DCC DMAP CHCl3 RTt 3 h
NTA
52
3-mercapto trimethoxysilane
(MPS)
3-amino trimethoxysilane
(APS)
5 Silane multifunctional monomers
Glycidoxypropyltrimethoxy-silane
(GPS)
53
54
Multifunctional magnetic nanoparticles
application
Bioseparation
Biodetection(Real time monitoring)
Targeted drug delivery
Magnetic fluid Hyperthermia therapy
55
1 Specific Bio-Separation
bull Magnetic properties
bull Targeting agent
Cell organism molecule
56
His-tagged protein purification as
the first step towards recombinant
protein purification
58
Lysing Binding
Nickel
affinity
nanoparicle
His tag
fusion protein
Washing Elusion
Digestion
Binding
Nickel
affinity
nanoparticle
Washing Target
Recombinant
Protein
1- Preparation of Fe3O4SiO2Layered Double Hydroxide CoreminusShell
Microspheres for Magnetic Separation of Proteins J Am ChemSoc (2012)
18 134(2) 1071-7
Ni2+
2- Magnetic Nanocomposite Spheres Decorated with NiO Nanoparticles for a
Magnetically Recyclable Protein Separation System J Kim Y Piao N Lee Y
Park I-H Lee J-H Lee Adv Mater (2010) 22 57ndash60
His-tagged protein purification
Purification of His6-lipase using Fe3O4AundashLAndashIDAndashCu2+ NPs with various
amounts of NPs (lane 1 01 mg lane 2 02 mg lane 3
63
Synthetic procedure for the coreshell structure spheres
64
glutaraldehyde
(GLH)
Reference[1] H W Gu Z M Yang J H Gao C K Chang B Xu J Am Chem Soc 2005 127 34
[2] A Hutten D Sudfeld I Ennen G Reiss K Wojczykowski P Jutzi J Magn Magn Mater 2005
293 93
[3] G S Chaubey C Barcena N Poudyal C B Rong J M Gao S Sun J P Liu J Am Chem
Soc 2007 129 7214
[4] C W Kim Y H Kim H G Cha D K Lee Y S Kang J NanosciNanotechnol 2006 6 3417
[5] Y W Jun J S Choi J Cheon Angew Chem Int Ed 2006 45 3414
[6] X Michalet F F Pinaud L A Bentolila J M Tsay S Doose J J Li G Sundaresan A M Wu
S S Gambhir S Weiss Science 2005 307 538
[7] M Hu J Y Chen Z Y Li L Au G V Hartland X D Li M Marquez Y N Xia Chem Soc
Rev 2006 35 1084
[8] M Vallet-Regi F Balas D Arcos Angew Chem Int Ed 2007 46 7548
[9] S T Selvan P K Patra C Y Ang J Y Ying Angew Chem Int Ed 2007 46 2448
[10] I S Lee N Lee J Park B H Kim Y W Yi T Kim T K Kim I H Lee S R Paik T
Hyeon J Am Chem Soc 2006 128 10658
[11] Y S Lin S H Wu Y Hung Y H Chou C Chang M L Lin C P Tsai C Y Mou Chem
Mater 2006 18 5170
[12] B H Wu H Zhang C Chen S C Lin N F Zheng Nano Res 2009 2 975
[13] J Kim J E Lee J Lee J H Yu B C Kim K An Y Hwang C Shin J G Park J Kim T
Hyeon J Am Chem Soc 2006 128 688
[14] S Santra H S Yang P H Holloway J T Stanley R A Mericle J Am Chem Soc 2005 127
1656
[15] D K Yi S T Selvan S S Lee G C Papaefthymiou D Kundaliya J Y Ying J Am Chem
Soc 2005 127 4990 65
[16] J H Gao B Zhang Y Gao Y Pan X X Zhang B Xu J Am Chem Soc 2007 129 11928 [17] J H Gao W Zhang P B Huang B Zhang X X Zhang B Xu J Am Chem Soc 2008 130 3710 [18] J H Gao G L Liang B Zhang Y Kuang X X Zhang B Xu J Am Chem Soc 2007 129 1428 [19] J H Gao G L Liang J S Cheung Y Pan Y Kuang F Zhao B Zhang X X Zhang E X Wu B Xu J Am Chem Soc 2008 130 11828 [20] A R Kortan R Hull R L Opila M G Bawendi M L Steigerwald P J Carroll L E Brus JAm Chem Soc 1990 112 1327 [21] X G Peng M C Schlamp A V Kadavanich A P Alivisatos J Am Chem Soc 1997 119 7019 [22] S Kim B Fisher H J Eisler M Bawendi J Am Chem Soc 2003 125 11466[23] H Zeng J Li Z L Wang J P Liu S Sun Nano Lett 2004 4 187 [24] H Zeng S Sun J Li Z L Wang J P Liu Appl Phys Lett 2004 85 792 [25] Z Xu Y Hou S Sun J Am Chem Soc 2007 129 8698 [26] Y H Lin N Kim J Q Wang C J Zhong J Phys Chem B 2005 109 21593 [27] C Wang H Daimon S Sun Nano Lett 2009 9 1493 [28] K W Kwon M Shim J Am Chem Soc 2005 127 10269 [29] H Yu M Chen P M Rice S X Wang R L White S Sun Nano Lett 2005 5 379 [30] J S Choi Y W Jun S I Yeon H C Kim J S Shin J Cheon J Am Chem Soc 2006 128 15982 [31] S H Choi H B Na Y I Park K An S G Kwon Y Jang M Park J Moon J S Son I CSong W K Moon T Hyeon J Am Chem Soc 2008 130 15573 [32] H W Gu R K Zheng X X Zhang B Xu J Am Chem Soc 2004 126 5664
66
La Mer theory
and
5
MNP synthetic methods
Coprecipitation
Sol-gel synthesis
Microemulsion synthesis
Sonochemical reaction
Hydrothermal reaction
Thermal decomposition
Electrospray synthesis
Laser pyrolysis
6
7
1- Coprecipitation methods
Morphology Size and Quantity of MNP have been
control by a series of experimental parameters such as
- pH
- Reaction temperature
- Precursor
Disadvantage Expanded size distribution
8
2- New modified Coprecipitation
Ref2 Milichko et al Nanoscale Research Letters 2013 8317 9
3- Modified Hydrothermal reaction
10
4- Thermal decomposition
MNP synthesized by the thermal decomposition of Fe(acac)3 in PEG and in MPEG
Tian XH Wei F Wang TX Wang P Int J Nanomedicine 201271031-4111
5- Sol-gel synthesis
Porous or hollow iron oxide nanostructures have
been synthesized by sol gel process
12
Wrapndash BakendashPeel process
Reduction
MagnetiteSilicaMagnetite
Removal of
silica using
NaOH
β-FeOOH β-FeOOHSilicaHematiteSilica
Removal of
silica using
NaOH
Hematite
Silica
coating
Heat
treatment
13
6- Kirkendall process(Nano hollow structures)
The rate of oxygen diffusion towards the core is slower than
diffusion of iron outwards from the core leading to the formation
of hollow structures
Fe
Fe3O4 Fe3O4
Fe
Fe3O4
Amorphous Crystalline
1 Oxidation by
(CH3)3NO
2 Heating
gt200oC
14
15
Colloidal stability by citrate
Au Au
- ---- - -
- ---O O
OO O
O
O H
Lowis bond
16
Color is size dependent
Size(nm) Emission(nm) Suspension in
5 515-520 PBSsurfactant
10 515-520 PBSsurfactant
15 520 PBSsurfactant
20 524 PBSsurfactant
30 526 PBSsurfactant
40 530 PBSsurfactant
50 535 PBSsurfactant
60 540 PBSsurfactant
80 553 PBSsurfactant
100 572 PBSsurfactant
17
18
GNP Advantage
- Color size dependent
- No toxicity
- DNA strong bonding
- Thiol covalent bond
19
The surface Hydrophobicity can be modified by
thiol compound
20
The surface self-assembling by thiol compound
21
GNP Application
Different bio sensor based self assemble design
22
23
The Sol-Gel Process is
a method for producing solid materials from small molecules
inn RT The method is used for the fabrication of metal oxides
especially the oxides of silicon and titanium
24
Alkoxide-sol-gel-method-preparation
25
HydrolysisCondensation
26
27
Why silica sol-gel coating
- Biocompatible
- Functionalization
- Physical trapping
- Chemical stability
- Physical stability
28
I Byun AW Coleman B Kim Micromech Microeng 23 (2013) 085016 29
Three Dimensional
Multi Dimensional matrix
One Dimensional
Layer by layer coatingSi
OR
ORRORO
Si
OR
RORO
NH2
Si
OR
RORO
SH
Si
OR
RORO O
O
n
Si
OR
RORO
HN
NH
NH2
Si
R
RRORO
30
Two Dimensional
Physical trapping in sol gel
31
32
apto ldquoto fitrdquo
mer ldquosmallest unit of repeating structurerdquo
What is an Aptamer
Oligonucleotide or Peptide
molecules that bind to a specific
target molecule
33
Types of Aptamer
- ssDNA
- dsDNA
- RNA
- Peptide
34
SELEX(Systematic Evolution of Ligands by Exponential Enrichment)
Selection of ligand sequences that bind to a target
partitioning of aptamers from
non-aptamers via affinity methods
amplification of bound aptamers
35
Targets of Aptamers
- Small Molecules
- Proteins
- Nucleic Acids
- Cells
- Tissues
- Organisms
lysozym Thrombin HIV TAR Hemin Interferon γ Vascular
endotherial growth factor (VEGF) Prostate specific antigen(PSA)
Dopamine hellip 36
37
MFMNP Design
Core Shell
Dumbbell Structure
Multicomponent Hybrid NP
38
39
Polymer Shell
Core Shell
Metal alloy
(FePt)
Magnetic nanolayer
(Fe3O4)
Thermal decomposition
of Fe(acac)3 on FePt
Magnetic NP
(Fe3O4)
Metal alloy
(Au Ag)
(Fe3O4) in Au3+ solution
at reduction condition
40
1 Core Shell structure
41
- Mix of NP during the growth of nanocrystals
- Sol-gel process
Core Shell synthesis
2 Dumbbell structures
42
- Mix of NP during the growth of nanocrystals
- Thermal rearrangement
- self-assemble at a liquid-liquid interface
- Functional sol-gel matrix
3 Multicomponent Hybrid NPs
Self-assembly processes provide an approach to
fabricating multicomponent hybrid NPs with
integrated multifunctionality
43
- Surfactant polymers
- Sol-gel process
44
Poly(lactico-co-glycolicacid)
(PLGA)
Poly(L-lysine)-PEG-folate
(PLL-PEG-FOL)
By mechanical
stirring at RT
in CH3Cl
45
46
W Lu SR Arumugam D Senapati ACSNANO 2010 4(3) 1739ndash1749
GNP GNP
GNP
RNA Aptamer
Anti-HER2C-reb-2 antibody
GNPCTAB
47
Magnetic nanoparticle surface modification Glucose oxidasse
(GOx)
PEGGPS
APSMNP
48Z Lin et al Analytica Chimica Acta 818 (2014) 54ndash60
49
P Sun H Zhang C Liu Langmuir 2010 26(2) 1278ndash1284 50
Immuno-labeling using Fe3O4CdTe nanocomposites which are formed by
linking multiple TGA-stabilized CdTe QDs with the thiol-functionalized silica
coated iron oxide nanoparticles
23-dimercaptosuccinic
acids (DMSA)Dopamine
Mercaptoundecanoic acid
(MUA)
HS COOH
4 Small multifunctional molecules
Glutaraldehyde 51
C J Xu K M Xu H W Gu R K Zheng H Liu X X Zhang Z H Guo B Xu J Am Chem Soc 2004 126 9938
Dopamine
Conditions (a) NaOH tert-butyl dicarbonate dioxane H2O 24 hrs (b) BnBr K2CO3 DMF
RT 24 h (c) 10 CF3COOH CH2Cl2 rt 5 h (d) succinic anhydride pyridine RTt 3 h (e)
NHS DCC DMAP CHCl3 RTt 3 h
NTA
52
3-mercapto trimethoxysilane
(MPS)
3-amino trimethoxysilane
(APS)
5 Silane multifunctional monomers
Glycidoxypropyltrimethoxy-silane
(GPS)
53
54
Multifunctional magnetic nanoparticles
application
Bioseparation
Biodetection(Real time monitoring)
Targeted drug delivery
Magnetic fluid Hyperthermia therapy
55
1 Specific Bio-Separation
bull Magnetic properties
bull Targeting agent
Cell organism molecule
56
His-tagged protein purification as
the first step towards recombinant
protein purification
58
Lysing Binding
Nickel
affinity
nanoparicle
His tag
fusion protein
Washing Elusion
Digestion
Binding
Nickel
affinity
nanoparticle
Washing Target
Recombinant
Protein
1- Preparation of Fe3O4SiO2Layered Double Hydroxide CoreminusShell
Microspheres for Magnetic Separation of Proteins J Am ChemSoc (2012)
18 134(2) 1071-7
Ni2+
2- Magnetic Nanocomposite Spheres Decorated with NiO Nanoparticles for a
Magnetically Recyclable Protein Separation System J Kim Y Piao N Lee Y
Park I-H Lee J-H Lee Adv Mater (2010) 22 57ndash60
His-tagged protein purification
Purification of His6-lipase using Fe3O4AundashLAndashIDAndashCu2+ NPs with various
amounts of NPs (lane 1 01 mg lane 2 02 mg lane 3
63
Synthetic procedure for the coreshell structure spheres
64
glutaraldehyde
(GLH)
Reference[1] H W Gu Z M Yang J H Gao C K Chang B Xu J Am Chem Soc 2005 127 34
[2] A Hutten D Sudfeld I Ennen G Reiss K Wojczykowski P Jutzi J Magn Magn Mater 2005
293 93
[3] G S Chaubey C Barcena N Poudyal C B Rong J M Gao S Sun J P Liu J Am Chem
Soc 2007 129 7214
[4] C W Kim Y H Kim H G Cha D K Lee Y S Kang J NanosciNanotechnol 2006 6 3417
[5] Y W Jun J S Choi J Cheon Angew Chem Int Ed 2006 45 3414
[6] X Michalet F F Pinaud L A Bentolila J M Tsay S Doose J J Li G Sundaresan A M Wu
S S Gambhir S Weiss Science 2005 307 538
[7] M Hu J Y Chen Z Y Li L Au G V Hartland X D Li M Marquez Y N Xia Chem Soc
Rev 2006 35 1084
[8] M Vallet-Regi F Balas D Arcos Angew Chem Int Ed 2007 46 7548
[9] S T Selvan P K Patra C Y Ang J Y Ying Angew Chem Int Ed 2007 46 2448
[10] I S Lee N Lee J Park B H Kim Y W Yi T Kim T K Kim I H Lee S R Paik T
Hyeon J Am Chem Soc 2006 128 10658
[11] Y S Lin S H Wu Y Hung Y H Chou C Chang M L Lin C P Tsai C Y Mou Chem
Mater 2006 18 5170
[12] B H Wu H Zhang C Chen S C Lin N F Zheng Nano Res 2009 2 975
[13] J Kim J E Lee J Lee J H Yu B C Kim K An Y Hwang C Shin J G Park J Kim T
Hyeon J Am Chem Soc 2006 128 688
[14] S Santra H S Yang P H Holloway J T Stanley R A Mericle J Am Chem Soc 2005 127
1656
[15] D K Yi S T Selvan S S Lee G C Papaefthymiou D Kundaliya J Y Ying J Am Chem
Soc 2005 127 4990 65
[16] J H Gao B Zhang Y Gao Y Pan X X Zhang B Xu J Am Chem Soc 2007 129 11928 [17] J H Gao W Zhang P B Huang B Zhang X X Zhang B Xu J Am Chem Soc 2008 130 3710 [18] J H Gao G L Liang B Zhang Y Kuang X X Zhang B Xu J Am Chem Soc 2007 129 1428 [19] J H Gao G L Liang J S Cheung Y Pan Y Kuang F Zhao B Zhang X X Zhang E X Wu B Xu J Am Chem Soc 2008 130 11828 [20] A R Kortan R Hull R L Opila M G Bawendi M L Steigerwald P J Carroll L E Brus JAm Chem Soc 1990 112 1327 [21] X G Peng M C Schlamp A V Kadavanich A P Alivisatos J Am Chem Soc 1997 119 7019 [22] S Kim B Fisher H J Eisler M Bawendi J Am Chem Soc 2003 125 11466[23] H Zeng J Li Z L Wang J P Liu S Sun Nano Lett 2004 4 187 [24] H Zeng S Sun J Li Z L Wang J P Liu Appl Phys Lett 2004 85 792 [25] Z Xu Y Hou S Sun J Am Chem Soc 2007 129 8698 [26] Y H Lin N Kim J Q Wang C J Zhong J Phys Chem B 2005 109 21593 [27] C Wang H Daimon S Sun Nano Lett 2009 9 1493 [28] K W Kwon M Shim J Am Chem Soc 2005 127 10269 [29] H Yu M Chen P M Rice S X Wang R L White S Sun Nano Lett 2005 5 379 [30] J S Choi Y W Jun S I Yeon H C Kim J S Shin J Cheon J Am Chem Soc 2006 128 15982 [31] S H Choi H B Na Y I Park K An S G Kwon Y Jang M Park J Moon J S Son I CSong W K Moon T Hyeon J Am Chem Soc 2008 130 15573 [32] H W Gu R K Zheng X X Zhang B Xu J Am Chem Soc 2004 126 5664
66
MNP synthetic methods
Coprecipitation
Sol-gel synthesis
Microemulsion synthesis
Sonochemical reaction
Hydrothermal reaction
Thermal decomposition
Electrospray synthesis
Laser pyrolysis
6
7
1- Coprecipitation methods
Morphology Size and Quantity of MNP have been
control by a series of experimental parameters such as
- pH
- Reaction temperature
- Precursor
Disadvantage Expanded size distribution
8
2- New modified Coprecipitation
Ref2 Milichko et al Nanoscale Research Letters 2013 8317 9
3- Modified Hydrothermal reaction
10
4- Thermal decomposition
MNP synthesized by the thermal decomposition of Fe(acac)3 in PEG and in MPEG
Tian XH Wei F Wang TX Wang P Int J Nanomedicine 201271031-4111
5- Sol-gel synthesis
Porous or hollow iron oxide nanostructures have
been synthesized by sol gel process
12
Wrapndash BakendashPeel process
Reduction
MagnetiteSilicaMagnetite
Removal of
silica using
NaOH
β-FeOOH β-FeOOHSilicaHematiteSilica
Removal of
silica using
NaOH
Hematite
Silica
coating
Heat
treatment
13
6- Kirkendall process(Nano hollow structures)
The rate of oxygen diffusion towards the core is slower than
diffusion of iron outwards from the core leading to the formation
of hollow structures
Fe
Fe3O4 Fe3O4
Fe
Fe3O4
Amorphous Crystalline
1 Oxidation by
(CH3)3NO
2 Heating
gt200oC
14
15
Colloidal stability by citrate
Au Au
- ---- - -
- ---O O
OO O
O
O H
Lowis bond
16
Color is size dependent
Size(nm) Emission(nm) Suspension in
5 515-520 PBSsurfactant
10 515-520 PBSsurfactant
15 520 PBSsurfactant
20 524 PBSsurfactant
30 526 PBSsurfactant
40 530 PBSsurfactant
50 535 PBSsurfactant
60 540 PBSsurfactant
80 553 PBSsurfactant
100 572 PBSsurfactant
17
18
GNP Advantage
- Color size dependent
- No toxicity
- DNA strong bonding
- Thiol covalent bond
19
The surface Hydrophobicity can be modified by
thiol compound
20
The surface self-assembling by thiol compound
21
GNP Application
Different bio sensor based self assemble design
22
23
The Sol-Gel Process is
a method for producing solid materials from small molecules
inn RT The method is used for the fabrication of metal oxides
especially the oxides of silicon and titanium
24
Alkoxide-sol-gel-method-preparation
25
HydrolysisCondensation
26
27
Why silica sol-gel coating
- Biocompatible
- Functionalization
- Physical trapping
- Chemical stability
- Physical stability
28
I Byun AW Coleman B Kim Micromech Microeng 23 (2013) 085016 29
Three Dimensional
Multi Dimensional matrix
One Dimensional
Layer by layer coatingSi
OR
ORRORO
Si
OR
RORO
NH2
Si
OR
RORO
SH
Si
OR
RORO O
O
n
Si
OR
RORO
HN
NH
NH2
Si
R
RRORO
30
Two Dimensional
Physical trapping in sol gel
31
32
apto ldquoto fitrdquo
mer ldquosmallest unit of repeating structurerdquo
What is an Aptamer
Oligonucleotide or Peptide
molecules that bind to a specific
target molecule
33
Types of Aptamer
- ssDNA
- dsDNA
- RNA
- Peptide
34
SELEX(Systematic Evolution of Ligands by Exponential Enrichment)
Selection of ligand sequences that bind to a target
partitioning of aptamers from
non-aptamers via affinity methods
amplification of bound aptamers
35
Targets of Aptamers
- Small Molecules
- Proteins
- Nucleic Acids
- Cells
- Tissues
- Organisms
lysozym Thrombin HIV TAR Hemin Interferon γ Vascular
endotherial growth factor (VEGF) Prostate specific antigen(PSA)
Dopamine hellip 36
37
MFMNP Design
Core Shell
Dumbbell Structure
Multicomponent Hybrid NP
38
39
Polymer Shell
Core Shell
Metal alloy
(FePt)
Magnetic nanolayer
(Fe3O4)
Thermal decomposition
of Fe(acac)3 on FePt
Magnetic NP
(Fe3O4)
Metal alloy
(Au Ag)
(Fe3O4) in Au3+ solution
at reduction condition
40
1 Core Shell structure
41
- Mix of NP during the growth of nanocrystals
- Sol-gel process
Core Shell synthesis
2 Dumbbell structures
42
- Mix of NP during the growth of nanocrystals
- Thermal rearrangement
- self-assemble at a liquid-liquid interface
- Functional sol-gel matrix
3 Multicomponent Hybrid NPs
Self-assembly processes provide an approach to
fabricating multicomponent hybrid NPs with
integrated multifunctionality
43
- Surfactant polymers
- Sol-gel process
44
Poly(lactico-co-glycolicacid)
(PLGA)
Poly(L-lysine)-PEG-folate
(PLL-PEG-FOL)
By mechanical
stirring at RT
in CH3Cl
45
46
W Lu SR Arumugam D Senapati ACSNANO 2010 4(3) 1739ndash1749
GNP GNP
GNP
RNA Aptamer
Anti-HER2C-reb-2 antibody
GNPCTAB
47
Magnetic nanoparticle surface modification Glucose oxidasse
(GOx)
PEGGPS
APSMNP
48Z Lin et al Analytica Chimica Acta 818 (2014) 54ndash60
49
P Sun H Zhang C Liu Langmuir 2010 26(2) 1278ndash1284 50
Immuno-labeling using Fe3O4CdTe nanocomposites which are formed by
linking multiple TGA-stabilized CdTe QDs with the thiol-functionalized silica
coated iron oxide nanoparticles
23-dimercaptosuccinic
acids (DMSA)Dopamine
Mercaptoundecanoic acid
(MUA)
HS COOH
4 Small multifunctional molecules
Glutaraldehyde 51
C J Xu K M Xu H W Gu R K Zheng H Liu X X Zhang Z H Guo B Xu J Am Chem Soc 2004 126 9938
Dopamine
Conditions (a) NaOH tert-butyl dicarbonate dioxane H2O 24 hrs (b) BnBr K2CO3 DMF
RT 24 h (c) 10 CF3COOH CH2Cl2 rt 5 h (d) succinic anhydride pyridine RTt 3 h (e)
NHS DCC DMAP CHCl3 RTt 3 h
NTA
52
3-mercapto trimethoxysilane
(MPS)
3-amino trimethoxysilane
(APS)
5 Silane multifunctional monomers
Glycidoxypropyltrimethoxy-silane
(GPS)
53
54
Multifunctional magnetic nanoparticles
application
Bioseparation
Biodetection(Real time monitoring)
Targeted drug delivery
Magnetic fluid Hyperthermia therapy
55
1 Specific Bio-Separation
bull Magnetic properties
bull Targeting agent
Cell organism molecule
56
His-tagged protein purification as
the first step towards recombinant
protein purification
58
Lysing Binding
Nickel
affinity
nanoparicle
His tag
fusion protein
Washing Elusion
Digestion
Binding
Nickel
affinity
nanoparticle
Washing Target
Recombinant
Protein
1- Preparation of Fe3O4SiO2Layered Double Hydroxide CoreminusShell
Microspheres for Magnetic Separation of Proteins J Am ChemSoc (2012)
18 134(2) 1071-7
Ni2+
2- Magnetic Nanocomposite Spheres Decorated with NiO Nanoparticles for a
Magnetically Recyclable Protein Separation System J Kim Y Piao N Lee Y
Park I-H Lee J-H Lee Adv Mater (2010) 22 57ndash60
His-tagged protein purification
Purification of His6-lipase using Fe3O4AundashLAndashIDAndashCu2+ NPs with various
amounts of NPs (lane 1 01 mg lane 2 02 mg lane 3
63
Synthetic procedure for the coreshell structure spheres
64
glutaraldehyde
(GLH)
Reference[1] H W Gu Z M Yang J H Gao C K Chang B Xu J Am Chem Soc 2005 127 34
[2] A Hutten D Sudfeld I Ennen G Reiss K Wojczykowski P Jutzi J Magn Magn Mater 2005
293 93
[3] G S Chaubey C Barcena N Poudyal C B Rong J M Gao S Sun J P Liu J Am Chem
Soc 2007 129 7214
[4] C W Kim Y H Kim H G Cha D K Lee Y S Kang J NanosciNanotechnol 2006 6 3417
[5] Y W Jun J S Choi J Cheon Angew Chem Int Ed 2006 45 3414
[6] X Michalet F F Pinaud L A Bentolila J M Tsay S Doose J J Li G Sundaresan A M Wu
S S Gambhir S Weiss Science 2005 307 538
[7] M Hu J Y Chen Z Y Li L Au G V Hartland X D Li M Marquez Y N Xia Chem Soc
Rev 2006 35 1084
[8] M Vallet-Regi F Balas D Arcos Angew Chem Int Ed 2007 46 7548
[9] S T Selvan P K Patra C Y Ang J Y Ying Angew Chem Int Ed 2007 46 2448
[10] I S Lee N Lee J Park B H Kim Y W Yi T Kim T K Kim I H Lee S R Paik T
Hyeon J Am Chem Soc 2006 128 10658
[11] Y S Lin S H Wu Y Hung Y H Chou C Chang M L Lin C P Tsai C Y Mou Chem
Mater 2006 18 5170
[12] B H Wu H Zhang C Chen S C Lin N F Zheng Nano Res 2009 2 975
[13] J Kim J E Lee J Lee J H Yu B C Kim K An Y Hwang C Shin J G Park J Kim T
Hyeon J Am Chem Soc 2006 128 688
[14] S Santra H S Yang P H Holloway J T Stanley R A Mericle J Am Chem Soc 2005 127
1656
[15] D K Yi S T Selvan S S Lee G C Papaefthymiou D Kundaliya J Y Ying J Am Chem
Soc 2005 127 4990 65
[16] J H Gao B Zhang Y Gao Y Pan X X Zhang B Xu J Am Chem Soc 2007 129 11928 [17] J H Gao W Zhang P B Huang B Zhang X X Zhang B Xu J Am Chem Soc 2008 130 3710 [18] J H Gao G L Liang B Zhang Y Kuang X X Zhang B Xu J Am Chem Soc 2007 129 1428 [19] J H Gao G L Liang J S Cheung Y Pan Y Kuang F Zhao B Zhang X X Zhang E X Wu B Xu J Am Chem Soc 2008 130 11828 [20] A R Kortan R Hull R L Opila M G Bawendi M L Steigerwald P J Carroll L E Brus JAm Chem Soc 1990 112 1327 [21] X G Peng M C Schlamp A V Kadavanich A P Alivisatos J Am Chem Soc 1997 119 7019 [22] S Kim B Fisher H J Eisler M Bawendi J Am Chem Soc 2003 125 11466[23] H Zeng J Li Z L Wang J P Liu S Sun Nano Lett 2004 4 187 [24] H Zeng S Sun J Li Z L Wang J P Liu Appl Phys Lett 2004 85 792 [25] Z Xu Y Hou S Sun J Am Chem Soc 2007 129 8698 [26] Y H Lin N Kim J Q Wang C J Zhong J Phys Chem B 2005 109 21593 [27] C Wang H Daimon S Sun Nano Lett 2009 9 1493 [28] K W Kwon M Shim J Am Chem Soc 2005 127 10269 [29] H Yu M Chen P M Rice S X Wang R L White S Sun Nano Lett 2005 5 379 [30] J S Choi Y W Jun S I Yeon H C Kim J S Shin J Cheon J Am Chem Soc 2006 128 15982 [31] S H Choi H B Na Y I Park K An S G Kwon Y Jang M Park J Moon J S Son I CSong W K Moon T Hyeon J Am Chem Soc 2008 130 15573 [32] H W Gu R K Zheng X X Zhang B Xu J Am Chem Soc 2004 126 5664
66
7
1- Coprecipitation methods
Morphology Size and Quantity of MNP have been
control by a series of experimental parameters such as
- pH
- Reaction temperature
- Precursor
Disadvantage Expanded size distribution
8
2- New modified Coprecipitation
Ref2 Milichko et al Nanoscale Research Letters 2013 8317 9
3- Modified Hydrothermal reaction
10
4- Thermal decomposition
MNP synthesized by the thermal decomposition of Fe(acac)3 in PEG and in MPEG
Tian XH Wei F Wang TX Wang P Int J Nanomedicine 201271031-4111
5- Sol-gel synthesis
Porous or hollow iron oxide nanostructures have
been synthesized by sol gel process
12
Wrapndash BakendashPeel process
Reduction
MagnetiteSilicaMagnetite
Removal of
silica using
NaOH
β-FeOOH β-FeOOHSilicaHematiteSilica
Removal of
silica using
NaOH
Hematite
Silica
coating
Heat
treatment
13
6- Kirkendall process(Nano hollow structures)
The rate of oxygen diffusion towards the core is slower than
diffusion of iron outwards from the core leading to the formation
of hollow structures
Fe
Fe3O4 Fe3O4
Fe
Fe3O4
Amorphous Crystalline
1 Oxidation by
(CH3)3NO
2 Heating
gt200oC
14
15
Colloidal stability by citrate
Au Au
- ---- - -
- ---O O
OO O
O
O H
Lowis bond
16
Color is size dependent
Size(nm) Emission(nm) Suspension in
5 515-520 PBSsurfactant
10 515-520 PBSsurfactant
15 520 PBSsurfactant
20 524 PBSsurfactant
30 526 PBSsurfactant
40 530 PBSsurfactant
50 535 PBSsurfactant
60 540 PBSsurfactant
80 553 PBSsurfactant
100 572 PBSsurfactant
17
18
GNP Advantage
- Color size dependent
- No toxicity
- DNA strong bonding
- Thiol covalent bond
19
The surface Hydrophobicity can be modified by
thiol compound
20
The surface self-assembling by thiol compound
21
GNP Application
Different bio sensor based self assemble design
22
23
The Sol-Gel Process is
a method for producing solid materials from small molecules
inn RT The method is used for the fabrication of metal oxides
especially the oxides of silicon and titanium
24
Alkoxide-sol-gel-method-preparation
25
HydrolysisCondensation
26
27
Why silica sol-gel coating
- Biocompatible
- Functionalization
- Physical trapping
- Chemical stability
- Physical stability
28
I Byun AW Coleman B Kim Micromech Microeng 23 (2013) 085016 29
Three Dimensional
Multi Dimensional matrix
One Dimensional
Layer by layer coatingSi
OR
ORRORO
Si
OR
RORO
NH2
Si
OR
RORO
SH
Si
OR
RORO O
O
n
Si
OR
RORO
HN
NH
NH2
Si
R
RRORO
30
Two Dimensional
Physical trapping in sol gel
31
32
apto ldquoto fitrdquo
mer ldquosmallest unit of repeating structurerdquo
What is an Aptamer
Oligonucleotide or Peptide
molecules that bind to a specific
target molecule
33
Types of Aptamer
- ssDNA
- dsDNA
- RNA
- Peptide
34
SELEX(Systematic Evolution of Ligands by Exponential Enrichment)
Selection of ligand sequences that bind to a target
partitioning of aptamers from
non-aptamers via affinity methods
amplification of bound aptamers
35
Targets of Aptamers
- Small Molecules
- Proteins
- Nucleic Acids
- Cells
- Tissues
- Organisms
lysozym Thrombin HIV TAR Hemin Interferon γ Vascular
endotherial growth factor (VEGF) Prostate specific antigen(PSA)
Dopamine hellip 36
37
MFMNP Design
Core Shell
Dumbbell Structure
Multicomponent Hybrid NP
38
39
Polymer Shell
Core Shell
Metal alloy
(FePt)
Magnetic nanolayer
(Fe3O4)
Thermal decomposition
of Fe(acac)3 on FePt
Magnetic NP
(Fe3O4)
Metal alloy
(Au Ag)
(Fe3O4) in Au3+ solution
at reduction condition
40
1 Core Shell structure
41
- Mix of NP during the growth of nanocrystals
- Sol-gel process
Core Shell synthesis
2 Dumbbell structures
42
- Mix of NP during the growth of nanocrystals
- Thermal rearrangement
- self-assemble at a liquid-liquid interface
- Functional sol-gel matrix
3 Multicomponent Hybrid NPs
Self-assembly processes provide an approach to
fabricating multicomponent hybrid NPs with
integrated multifunctionality
43
- Surfactant polymers
- Sol-gel process
44
Poly(lactico-co-glycolicacid)
(PLGA)
Poly(L-lysine)-PEG-folate
(PLL-PEG-FOL)
By mechanical
stirring at RT
in CH3Cl
45
46
W Lu SR Arumugam D Senapati ACSNANO 2010 4(3) 1739ndash1749
GNP GNP
GNP
RNA Aptamer
Anti-HER2C-reb-2 antibody
GNPCTAB
47
Magnetic nanoparticle surface modification Glucose oxidasse
(GOx)
PEGGPS
APSMNP
48Z Lin et al Analytica Chimica Acta 818 (2014) 54ndash60
49
P Sun H Zhang C Liu Langmuir 2010 26(2) 1278ndash1284 50
Immuno-labeling using Fe3O4CdTe nanocomposites which are formed by
linking multiple TGA-stabilized CdTe QDs with the thiol-functionalized silica
coated iron oxide nanoparticles
23-dimercaptosuccinic
acids (DMSA)Dopamine
Mercaptoundecanoic acid
(MUA)
HS COOH
4 Small multifunctional molecules
Glutaraldehyde 51
C J Xu K M Xu H W Gu R K Zheng H Liu X X Zhang Z H Guo B Xu J Am Chem Soc 2004 126 9938
Dopamine
Conditions (a) NaOH tert-butyl dicarbonate dioxane H2O 24 hrs (b) BnBr K2CO3 DMF
RT 24 h (c) 10 CF3COOH CH2Cl2 rt 5 h (d) succinic anhydride pyridine RTt 3 h (e)
NHS DCC DMAP CHCl3 RTt 3 h
NTA
52
3-mercapto trimethoxysilane
(MPS)
3-amino trimethoxysilane
(APS)
5 Silane multifunctional monomers
Glycidoxypropyltrimethoxy-silane
(GPS)
53
54
Multifunctional magnetic nanoparticles
application
Bioseparation
Biodetection(Real time monitoring)
Targeted drug delivery
Magnetic fluid Hyperthermia therapy
55
1 Specific Bio-Separation
bull Magnetic properties
bull Targeting agent
Cell organism molecule
56
His-tagged protein purification as
the first step towards recombinant
protein purification
58
Lysing Binding
Nickel
affinity
nanoparicle
His tag
fusion protein
Washing Elusion
Digestion
Binding
Nickel
affinity
nanoparticle
Washing Target
Recombinant
Protein
1- Preparation of Fe3O4SiO2Layered Double Hydroxide CoreminusShell
Microspheres for Magnetic Separation of Proteins J Am ChemSoc (2012)
18 134(2) 1071-7
Ni2+
2- Magnetic Nanocomposite Spheres Decorated with NiO Nanoparticles for a
Magnetically Recyclable Protein Separation System J Kim Y Piao N Lee Y
Park I-H Lee J-H Lee Adv Mater (2010) 22 57ndash60
His-tagged protein purification
Purification of His6-lipase using Fe3O4AundashLAndashIDAndashCu2+ NPs with various
amounts of NPs (lane 1 01 mg lane 2 02 mg lane 3
63
Synthetic procedure for the coreshell structure spheres
64
glutaraldehyde
(GLH)
Reference[1] H W Gu Z M Yang J H Gao C K Chang B Xu J Am Chem Soc 2005 127 34
[2] A Hutten D Sudfeld I Ennen G Reiss K Wojczykowski P Jutzi J Magn Magn Mater 2005
293 93
[3] G S Chaubey C Barcena N Poudyal C B Rong J M Gao S Sun J P Liu J Am Chem
Soc 2007 129 7214
[4] C W Kim Y H Kim H G Cha D K Lee Y S Kang J NanosciNanotechnol 2006 6 3417
[5] Y W Jun J S Choi J Cheon Angew Chem Int Ed 2006 45 3414
[6] X Michalet F F Pinaud L A Bentolila J M Tsay S Doose J J Li G Sundaresan A M Wu
S S Gambhir S Weiss Science 2005 307 538
[7] M Hu J Y Chen Z Y Li L Au G V Hartland X D Li M Marquez Y N Xia Chem Soc
Rev 2006 35 1084
[8] M Vallet-Regi F Balas D Arcos Angew Chem Int Ed 2007 46 7548
[9] S T Selvan P K Patra C Y Ang J Y Ying Angew Chem Int Ed 2007 46 2448
[10] I S Lee N Lee J Park B H Kim Y W Yi T Kim T K Kim I H Lee S R Paik T
Hyeon J Am Chem Soc 2006 128 10658
[11] Y S Lin S H Wu Y Hung Y H Chou C Chang M L Lin C P Tsai C Y Mou Chem
Mater 2006 18 5170
[12] B H Wu H Zhang C Chen S C Lin N F Zheng Nano Res 2009 2 975
[13] J Kim J E Lee J Lee J H Yu B C Kim K An Y Hwang C Shin J G Park J Kim T
Hyeon J Am Chem Soc 2006 128 688
[14] S Santra H S Yang P H Holloway J T Stanley R A Mericle J Am Chem Soc 2005 127
1656
[15] D K Yi S T Selvan S S Lee G C Papaefthymiou D Kundaliya J Y Ying J Am Chem
Soc 2005 127 4990 65
[16] J H Gao B Zhang Y Gao Y Pan X X Zhang B Xu J Am Chem Soc 2007 129 11928 [17] J H Gao W Zhang P B Huang B Zhang X X Zhang B Xu J Am Chem Soc 2008 130 3710 [18] J H Gao G L Liang B Zhang Y Kuang X X Zhang B Xu J Am Chem Soc 2007 129 1428 [19] J H Gao G L Liang J S Cheung Y Pan Y Kuang F Zhao B Zhang X X Zhang E X Wu B Xu J Am Chem Soc 2008 130 11828 [20] A R Kortan R Hull R L Opila M G Bawendi M L Steigerwald P J Carroll L E Brus JAm Chem Soc 1990 112 1327 [21] X G Peng M C Schlamp A V Kadavanich A P Alivisatos J Am Chem Soc 1997 119 7019 [22] S Kim B Fisher H J Eisler M Bawendi J Am Chem Soc 2003 125 11466[23] H Zeng J Li Z L Wang J P Liu S Sun Nano Lett 2004 4 187 [24] H Zeng S Sun J Li Z L Wang J P Liu Appl Phys Lett 2004 85 792 [25] Z Xu Y Hou S Sun J Am Chem Soc 2007 129 8698 [26] Y H Lin N Kim J Q Wang C J Zhong J Phys Chem B 2005 109 21593 [27] C Wang H Daimon S Sun Nano Lett 2009 9 1493 [28] K W Kwon M Shim J Am Chem Soc 2005 127 10269 [29] H Yu M Chen P M Rice S X Wang R L White S Sun Nano Lett 2005 5 379 [30] J S Choi Y W Jun S I Yeon H C Kim J S Shin J Cheon J Am Chem Soc 2006 128 15982 [31] S H Choi H B Na Y I Park K An S G Kwon Y Jang M Park J Moon J S Son I CSong W K Moon T Hyeon J Am Chem Soc 2008 130 15573 [32] H W Gu R K Zheng X X Zhang B Xu J Am Chem Soc 2004 126 5664
66
1- Coprecipitation methods
Morphology Size and Quantity of MNP have been
control by a series of experimental parameters such as
- pH
- Reaction temperature
- Precursor
Disadvantage Expanded size distribution
8
2- New modified Coprecipitation
Ref2 Milichko et al Nanoscale Research Letters 2013 8317 9
3- Modified Hydrothermal reaction
10
4- Thermal decomposition
MNP synthesized by the thermal decomposition of Fe(acac)3 in PEG and in MPEG
Tian XH Wei F Wang TX Wang P Int J Nanomedicine 201271031-4111
5- Sol-gel synthesis
Porous or hollow iron oxide nanostructures have
been synthesized by sol gel process
12
Wrapndash BakendashPeel process
Reduction
MagnetiteSilicaMagnetite
Removal of
silica using
NaOH
β-FeOOH β-FeOOHSilicaHematiteSilica
Removal of
silica using
NaOH
Hematite
Silica
coating
Heat
treatment
13
6- Kirkendall process(Nano hollow structures)
The rate of oxygen diffusion towards the core is slower than
diffusion of iron outwards from the core leading to the formation
of hollow structures
Fe
Fe3O4 Fe3O4
Fe
Fe3O4
Amorphous Crystalline
1 Oxidation by
(CH3)3NO
2 Heating
gt200oC
14
15
Colloidal stability by citrate
Au Au
- ---- - -
- ---O O
OO O
O
O H
Lowis bond
16
Color is size dependent
Size(nm) Emission(nm) Suspension in
5 515-520 PBSsurfactant
10 515-520 PBSsurfactant
15 520 PBSsurfactant
20 524 PBSsurfactant
30 526 PBSsurfactant
40 530 PBSsurfactant
50 535 PBSsurfactant
60 540 PBSsurfactant
80 553 PBSsurfactant
100 572 PBSsurfactant
17
18
GNP Advantage
- Color size dependent
- No toxicity
- DNA strong bonding
- Thiol covalent bond
19
The surface Hydrophobicity can be modified by
thiol compound
20
The surface self-assembling by thiol compound
21
GNP Application
Different bio sensor based self assemble design
22
23
The Sol-Gel Process is
a method for producing solid materials from small molecules
inn RT The method is used for the fabrication of metal oxides
especially the oxides of silicon and titanium
24
Alkoxide-sol-gel-method-preparation
25
HydrolysisCondensation
26
27
Why silica sol-gel coating
- Biocompatible
- Functionalization
- Physical trapping
- Chemical stability
- Physical stability
28
I Byun AW Coleman B Kim Micromech Microeng 23 (2013) 085016 29
Three Dimensional
Multi Dimensional matrix
One Dimensional
Layer by layer coatingSi
OR
ORRORO
Si
OR
RORO
NH2
Si
OR
RORO
SH
Si
OR
RORO O
O
n
Si
OR
RORO
HN
NH
NH2
Si
R
RRORO
30
Two Dimensional
Physical trapping in sol gel
31
32
apto ldquoto fitrdquo
mer ldquosmallest unit of repeating structurerdquo
What is an Aptamer
Oligonucleotide or Peptide
molecules that bind to a specific
target molecule
33
Types of Aptamer
- ssDNA
- dsDNA
- RNA
- Peptide
34
SELEX(Systematic Evolution of Ligands by Exponential Enrichment)
Selection of ligand sequences that bind to a target
partitioning of aptamers from
non-aptamers via affinity methods
amplification of bound aptamers
35
Targets of Aptamers
- Small Molecules
- Proteins
- Nucleic Acids
- Cells
- Tissues
- Organisms
lysozym Thrombin HIV TAR Hemin Interferon γ Vascular
endotherial growth factor (VEGF) Prostate specific antigen(PSA)
Dopamine hellip 36
37
MFMNP Design
Core Shell
Dumbbell Structure
Multicomponent Hybrid NP
38
39
Polymer Shell
Core Shell
Metal alloy
(FePt)
Magnetic nanolayer
(Fe3O4)
Thermal decomposition
of Fe(acac)3 on FePt
Magnetic NP
(Fe3O4)
Metal alloy
(Au Ag)
(Fe3O4) in Au3+ solution
at reduction condition
40
1 Core Shell structure
41
- Mix of NP during the growth of nanocrystals
- Sol-gel process
Core Shell synthesis
2 Dumbbell structures
42
- Mix of NP during the growth of nanocrystals
- Thermal rearrangement
- self-assemble at a liquid-liquid interface
- Functional sol-gel matrix
3 Multicomponent Hybrid NPs
Self-assembly processes provide an approach to
fabricating multicomponent hybrid NPs with
integrated multifunctionality
43
- Surfactant polymers
- Sol-gel process
44
Poly(lactico-co-glycolicacid)
(PLGA)
Poly(L-lysine)-PEG-folate
(PLL-PEG-FOL)
By mechanical
stirring at RT
in CH3Cl
45
46
W Lu SR Arumugam D Senapati ACSNANO 2010 4(3) 1739ndash1749
GNP GNP
GNP
RNA Aptamer
Anti-HER2C-reb-2 antibody
GNPCTAB
47
Magnetic nanoparticle surface modification Glucose oxidasse
(GOx)
PEGGPS
APSMNP
48Z Lin et al Analytica Chimica Acta 818 (2014) 54ndash60
49
P Sun H Zhang C Liu Langmuir 2010 26(2) 1278ndash1284 50
Immuno-labeling using Fe3O4CdTe nanocomposites which are formed by
linking multiple TGA-stabilized CdTe QDs with the thiol-functionalized silica
coated iron oxide nanoparticles
23-dimercaptosuccinic
acids (DMSA)Dopamine
Mercaptoundecanoic acid
(MUA)
HS COOH
4 Small multifunctional molecules
Glutaraldehyde 51
C J Xu K M Xu H W Gu R K Zheng H Liu X X Zhang Z H Guo B Xu J Am Chem Soc 2004 126 9938
Dopamine
Conditions (a) NaOH tert-butyl dicarbonate dioxane H2O 24 hrs (b) BnBr K2CO3 DMF
RT 24 h (c) 10 CF3COOH CH2Cl2 rt 5 h (d) succinic anhydride pyridine RTt 3 h (e)
NHS DCC DMAP CHCl3 RTt 3 h
NTA
52
3-mercapto trimethoxysilane
(MPS)
3-amino trimethoxysilane
(APS)
5 Silane multifunctional monomers
Glycidoxypropyltrimethoxy-silane
(GPS)
53
54
Multifunctional magnetic nanoparticles
application
Bioseparation
Biodetection(Real time monitoring)
Targeted drug delivery
Magnetic fluid Hyperthermia therapy
55
1 Specific Bio-Separation
bull Magnetic properties
bull Targeting agent
Cell organism molecule
56
His-tagged protein purification as
the first step towards recombinant
protein purification
58
Lysing Binding
Nickel
affinity
nanoparicle
His tag
fusion protein
Washing Elusion
Digestion
Binding
Nickel
affinity
nanoparticle
Washing Target
Recombinant
Protein
1- Preparation of Fe3O4SiO2Layered Double Hydroxide CoreminusShell
Microspheres for Magnetic Separation of Proteins J Am ChemSoc (2012)
18 134(2) 1071-7
Ni2+
2- Magnetic Nanocomposite Spheres Decorated with NiO Nanoparticles for a
Magnetically Recyclable Protein Separation System J Kim Y Piao N Lee Y
Park I-H Lee J-H Lee Adv Mater (2010) 22 57ndash60
His-tagged protein purification
Purification of His6-lipase using Fe3O4AundashLAndashIDAndashCu2+ NPs with various
amounts of NPs (lane 1 01 mg lane 2 02 mg lane 3
63
Synthetic procedure for the coreshell structure spheres
64
glutaraldehyde
(GLH)
Reference[1] H W Gu Z M Yang J H Gao C K Chang B Xu J Am Chem Soc 2005 127 34
[2] A Hutten D Sudfeld I Ennen G Reiss K Wojczykowski P Jutzi J Magn Magn Mater 2005
293 93
[3] G S Chaubey C Barcena N Poudyal C B Rong J M Gao S Sun J P Liu J Am Chem
Soc 2007 129 7214
[4] C W Kim Y H Kim H G Cha D K Lee Y S Kang J NanosciNanotechnol 2006 6 3417
[5] Y W Jun J S Choi J Cheon Angew Chem Int Ed 2006 45 3414
[6] X Michalet F F Pinaud L A Bentolila J M Tsay S Doose J J Li G Sundaresan A M Wu
S S Gambhir S Weiss Science 2005 307 538
[7] M Hu J Y Chen Z Y Li L Au G V Hartland X D Li M Marquez Y N Xia Chem Soc
Rev 2006 35 1084
[8] M Vallet-Regi F Balas D Arcos Angew Chem Int Ed 2007 46 7548
[9] S T Selvan P K Patra C Y Ang J Y Ying Angew Chem Int Ed 2007 46 2448
[10] I S Lee N Lee J Park B H Kim Y W Yi T Kim T K Kim I H Lee S R Paik T
Hyeon J Am Chem Soc 2006 128 10658
[11] Y S Lin S H Wu Y Hung Y H Chou C Chang M L Lin C P Tsai C Y Mou Chem
Mater 2006 18 5170
[12] B H Wu H Zhang C Chen S C Lin N F Zheng Nano Res 2009 2 975
[13] J Kim J E Lee J Lee J H Yu B C Kim K An Y Hwang C Shin J G Park J Kim T
Hyeon J Am Chem Soc 2006 128 688
[14] S Santra H S Yang P H Holloway J T Stanley R A Mericle J Am Chem Soc 2005 127
1656
[15] D K Yi S T Selvan S S Lee G C Papaefthymiou D Kundaliya J Y Ying J Am Chem
Soc 2005 127 4990 65
[16] J H Gao B Zhang Y Gao Y Pan X X Zhang B Xu J Am Chem Soc 2007 129 11928 [17] J H Gao W Zhang P B Huang B Zhang X X Zhang B Xu J Am Chem Soc 2008 130 3710 [18] J H Gao G L Liang B Zhang Y Kuang X X Zhang B Xu J Am Chem Soc 2007 129 1428 [19] J H Gao G L Liang J S Cheung Y Pan Y Kuang F Zhao B Zhang X X Zhang E X Wu B Xu J Am Chem Soc 2008 130 11828 [20] A R Kortan R Hull R L Opila M G Bawendi M L Steigerwald P J Carroll L E Brus JAm Chem Soc 1990 112 1327 [21] X G Peng M C Schlamp A V Kadavanich A P Alivisatos J Am Chem Soc 1997 119 7019 [22] S Kim B Fisher H J Eisler M Bawendi J Am Chem Soc 2003 125 11466[23] H Zeng J Li Z L Wang J P Liu S Sun Nano Lett 2004 4 187 [24] H Zeng S Sun J Li Z L Wang J P Liu Appl Phys Lett 2004 85 792 [25] Z Xu Y Hou S Sun J Am Chem Soc 2007 129 8698 [26] Y H Lin N Kim J Q Wang C J Zhong J Phys Chem B 2005 109 21593 [27] C Wang H Daimon S Sun Nano Lett 2009 9 1493 [28] K W Kwon M Shim J Am Chem Soc 2005 127 10269 [29] H Yu M Chen P M Rice S X Wang R L White S Sun Nano Lett 2005 5 379 [30] J S Choi Y W Jun S I Yeon H C Kim J S Shin J Cheon J Am Chem Soc 2006 128 15982 [31] S H Choi H B Na Y I Park K An S G Kwon Y Jang M Park J Moon J S Son I CSong W K Moon T Hyeon J Am Chem Soc 2008 130 15573 [32] H W Gu R K Zheng X X Zhang B Xu J Am Chem Soc 2004 126 5664
66
2- New modified Coprecipitation
Ref2 Milichko et al Nanoscale Research Letters 2013 8317 9
3- Modified Hydrothermal reaction
10
4- Thermal decomposition
MNP synthesized by the thermal decomposition of Fe(acac)3 in PEG and in MPEG
Tian XH Wei F Wang TX Wang P Int J Nanomedicine 201271031-4111
5- Sol-gel synthesis
Porous or hollow iron oxide nanostructures have
been synthesized by sol gel process
12
Wrapndash BakendashPeel process
Reduction
MagnetiteSilicaMagnetite
Removal of
silica using
NaOH
β-FeOOH β-FeOOHSilicaHematiteSilica
Removal of
silica using
NaOH
Hematite
Silica
coating
Heat
treatment
13
6- Kirkendall process(Nano hollow structures)
The rate of oxygen diffusion towards the core is slower than
diffusion of iron outwards from the core leading to the formation
of hollow structures
Fe
Fe3O4 Fe3O4
Fe
Fe3O4
Amorphous Crystalline
1 Oxidation by
(CH3)3NO
2 Heating
gt200oC
14
15
Colloidal stability by citrate
Au Au
- ---- - -
- ---O O
OO O
O
O H
Lowis bond
16
Color is size dependent
Size(nm) Emission(nm) Suspension in
5 515-520 PBSsurfactant
10 515-520 PBSsurfactant
15 520 PBSsurfactant
20 524 PBSsurfactant
30 526 PBSsurfactant
40 530 PBSsurfactant
50 535 PBSsurfactant
60 540 PBSsurfactant
80 553 PBSsurfactant
100 572 PBSsurfactant
17
18
GNP Advantage
- Color size dependent
- No toxicity
- DNA strong bonding
- Thiol covalent bond
19
The surface Hydrophobicity can be modified by
thiol compound
20
The surface self-assembling by thiol compound
21
GNP Application
Different bio sensor based self assemble design
22
23
The Sol-Gel Process is
a method for producing solid materials from small molecules
inn RT The method is used for the fabrication of metal oxides
especially the oxides of silicon and titanium
24
Alkoxide-sol-gel-method-preparation
25
HydrolysisCondensation
26
27
Why silica sol-gel coating
- Biocompatible
- Functionalization
- Physical trapping
- Chemical stability
- Physical stability
28
I Byun AW Coleman B Kim Micromech Microeng 23 (2013) 085016 29
Three Dimensional
Multi Dimensional matrix
One Dimensional
Layer by layer coatingSi
OR
ORRORO
Si
OR
RORO
NH2
Si
OR
RORO
SH
Si
OR
RORO O
O
n
Si
OR
RORO
HN
NH
NH2
Si
R
RRORO
30
Two Dimensional
Physical trapping in sol gel
31
32
apto ldquoto fitrdquo
mer ldquosmallest unit of repeating structurerdquo
What is an Aptamer
Oligonucleotide or Peptide
molecules that bind to a specific
target molecule
33
Types of Aptamer
- ssDNA
- dsDNA
- RNA
- Peptide
34
SELEX(Systematic Evolution of Ligands by Exponential Enrichment)
Selection of ligand sequences that bind to a target
partitioning of aptamers from
non-aptamers via affinity methods
amplification of bound aptamers
35
Targets of Aptamers
- Small Molecules
- Proteins
- Nucleic Acids
- Cells
- Tissues
- Organisms
lysozym Thrombin HIV TAR Hemin Interferon γ Vascular
endotherial growth factor (VEGF) Prostate specific antigen(PSA)
Dopamine hellip 36
37
MFMNP Design
Core Shell
Dumbbell Structure
Multicomponent Hybrid NP
38
39
Polymer Shell
Core Shell
Metal alloy
(FePt)
Magnetic nanolayer
(Fe3O4)
Thermal decomposition
of Fe(acac)3 on FePt
Magnetic NP
(Fe3O4)
Metal alloy
(Au Ag)
(Fe3O4) in Au3+ solution
at reduction condition
40
1 Core Shell structure
41
- Mix of NP during the growth of nanocrystals
- Sol-gel process
Core Shell synthesis
2 Dumbbell structures
42
- Mix of NP during the growth of nanocrystals
- Thermal rearrangement
- self-assemble at a liquid-liquid interface
- Functional sol-gel matrix
3 Multicomponent Hybrid NPs
Self-assembly processes provide an approach to
fabricating multicomponent hybrid NPs with
integrated multifunctionality
43
- Surfactant polymers
- Sol-gel process
44
Poly(lactico-co-glycolicacid)
(PLGA)
Poly(L-lysine)-PEG-folate
(PLL-PEG-FOL)
By mechanical
stirring at RT
in CH3Cl
45
46
W Lu SR Arumugam D Senapati ACSNANO 2010 4(3) 1739ndash1749
GNP GNP
GNP
RNA Aptamer
Anti-HER2C-reb-2 antibody
GNPCTAB
47
Magnetic nanoparticle surface modification Glucose oxidasse
(GOx)
PEGGPS
APSMNP
48Z Lin et al Analytica Chimica Acta 818 (2014) 54ndash60
49
P Sun H Zhang C Liu Langmuir 2010 26(2) 1278ndash1284 50
Immuno-labeling using Fe3O4CdTe nanocomposites which are formed by
linking multiple TGA-stabilized CdTe QDs with the thiol-functionalized silica
coated iron oxide nanoparticles
23-dimercaptosuccinic
acids (DMSA)Dopamine
Mercaptoundecanoic acid
(MUA)
HS COOH
4 Small multifunctional molecules
Glutaraldehyde 51
C J Xu K M Xu H W Gu R K Zheng H Liu X X Zhang Z H Guo B Xu J Am Chem Soc 2004 126 9938
Dopamine
Conditions (a) NaOH tert-butyl dicarbonate dioxane H2O 24 hrs (b) BnBr K2CO3 DMF
RT 24 h (c) 10 CF3COOH CH2Cl2 rt 5 h (d) succinic anhydride pyridine RTt 3 h (e)
NHS DCC DMAP CHCl3 RTt 3 h
NTA
52
3-mercapto trimethoxysilane
(MPS)
3-amino trimethoxysilane
(APS)
5 Silane multifunctional monomers
Glycidoxypropyltrimethoxy-silane
(GPS)
53
54
Multifunctional magnetic nanoparticles
application
Bioseparation
Biodetection(Real time monitoring)
Targeted drug delivery
Magnetic fluid Hyperthermia therapy
55
1 Specific Bio-Separation
bull Magnetic properties
bull Targeting agent
Cell organism molecule
56
His-tagged protein purification as
the first step towards recombinant
protein purification
58
Lysing Binding
Nickel
affinity
nanoparicle
His tag
fusion protein
Washing Elusion
Digestion
Binding
Nickel
affinity
nanoparticle
Washing Target
Recombinant
Protein
1- Preparation of Fe3O4SiO2Layered Double Hydroxide CoreminusShell
Microspheres for Magnetic Separation of Proteins J Am ChemSoc (2012)
18 134(2) 1071-7
Ni2+
2- Magnetic Nanocomposite Spheres Decorated with NiO Nanoparticles for a
Magnetically Recyclable Protein Separation System J Kim Y Piao N Lee Y
Park I-H Lee J-H Lee Adv Mater (2010) 22 57ndash60
His-tagged protein purification
Purification of His6-lipase using Fe3O4AundashLAndashIDAndashCu2+ NPs with various
amounts of NPs (lane 1 01 mg lane 2 02 mg lane 3
63
Synthetic procedure for the coreshell structure spheres
64
glutaraldehyde
(GLH)
Reference[1] H W Gu Z M Yang J H Gao C K Chang B Xu J Am Chem Soc 2005 127 34
[2] A Hutten D Sudfeld I Ennen G Reiss K Wojczykowski P Jutzi J Magn Magn Mater 2005
293 93
[3] G S Chaubey C Barcena N Poudyal C B Rong J M Gao S Sun J P Liu J Am Chem
Soc 2007 129 7214
[4] C W Kim Y H Kim H G Cha D K Lee Y S Kang J NanosciNanotechnol 2006 6 3417
[5] Y W Jun J S Choi J Cheon Angew Chem Int Ed 2006 45 3414
[6] X Michalet F F Pinaud L A Bentolila J M Tsay S Doose J J Li G Sundaresan A M Wu
S S Gambhir S Weiss Science 2005 307 538
[7] M Hu J Y Chen Z Y Li L Au G V Hartland X D Li M Marquez Y N Xia Chem Soc
Rev 2006 35 1084
[8] M Vallet-Regi F Balas D Arcos Angew Chem Int Ed 2007 46 7548
[9] S T Selvan P K Patra C Y Ang J Y Ying Angew Chem Int Ed 2007 46 2448
[10] I S Lee N Lee J Park B H Kim Y W Yi T Kim T K Kim I H Lee S R Paik T
Hyeon J Am Chem Soc 2006 128 10658
[11] Y S Lin S H Wu Y Hung Y H Chou C Chang M L Lin C P Tsai C Y Mou Chem
Mater 2006 18 5170
[12] B H Wu H Zhang C Chen S C Lin N F Zheng Nano Res 2009 2 975
[13] J Kim J E Lee J Lee J H Yu B C Kim K An Y Hwang C Shin J G Park J Kim T
Hyeon J Am Chem Soc 2006 128 688
[14] S Santra H S Yang P H Holloway J T Stanley R A Mericle J Am Chem Soc 2005 127
1656
[15] D K Yi S T Selvan S S Lee G C Papaefthymiou D Kundaliya J Y Ying J Am Chem
Soc 2005 127 4990 65
[16] J H Gao B Zhang Y Gao Y Pan X X Zhang B Xu J Am Chem Soc 2007 129 11928 [17] J H Gao W Zhang P B Huang B Zhang X X Zhang B Xu J Am Chem Soc 2008 130 3710 [18] J H Gao G L Liang B Zhang Y Kuang X X Zhang B Xu J Am Chem Soc 2007 129 1428 [19] J H Gao G L Liang J S Cheung Y Pan Y Kuang F Zhao B Zhang X X Zhang E X Wu B Xu J Am Chem Soc 2008 130 11828 [20] A R Kortan R Hull R L Opila M G Bawendi M L Steigerwald P J Carroll L E Brus JAm Chem Soc 1990 112 1327 [21] X G Peng M C Schlamp A V Kadavanich A P Alivisatos J Am Chem Soc 1997 119 7019 [22] S Kim B Fisher H J Eisler M Bawendi J Am Chem Soc 2003 125 11466[23] H Zeng J Li Z L Wang J P Liu S Sun Nano Lett 2004 4 187 [24] H Zeng S Sun J Li Z L Wang J P Liu Appl Phys Lett 2004 85 792 [25] Z Xu Y Hou S Sun J Am Chem Soc 2007 129 8698 [26] Y H Lin N Kim J Q Wang C J Zhong J Phys Chem B 2005 109 21593 [27] C Wang H Daimon S Sun Nano Lett 2009 9 1493 [28] K W Kwon M Shim J Am Chem Soc 2005 127 10269 [29] H Yu M Chen P M Rice S X Wang R L White S Sun Nano Lett 2005 5 379 [30] J S Choi Y W Jun S I Yeon H C Kim J S Shin J Cheon J Am Chem Soc 2006 128 15982 [31] S H Choi H B Na Y I Park K An S G Kwon Y Jang M Park J Moon J S Son I CSong W K Moon T Hyeon J Am Chem Soc 2008 130 15573 [32] H W Gu R K Zheng X X Zhang B Xu J Am Chem Soc 2004 126 5664
66
3- Modified Hydrothermal reaction
10
4- Thermal decomposition
MNP synthesized by the thermal decomposition of Fe(acac)3 in PEG and in MPEG
Tian XH Wei F Wang TX Wang P Int J Nanomedicine 201271031-4111
5- Sol-gel synthesis
Porous or hollow iron oxide nanostructures have
been synthesized by sol gel process
12
Wrapndash BakendashPeel process
Reduction
MagnetiteSilicaMagnetite
Removal of
silica using
NaOH
β-FeOOH β-FeOOHSilicaHematiteSilica
Removal of
silica using
NaOH
Hematite
Silica
coating
Heat
treatment
13
6- Kirkendall process(Nano hollow structures)
The rate of oxygen diffusion towards the core is slower than
diffusion of iron outwards from the core leading to the formation
of hollow structures
Fe
Fe3O4 Fe3O4
Fe
Fe3O4
Amorphous Crystalline
1 Oxidation by
(CH3)3NO
2 Heating
gt200oC
14
15
Colloidal stability by citrate
Au Au
- ---- - -
- ---O O
OO O
O
O H
Lowis bond
16
Color is size dependent
Size(nm) Emission(nm) Suspension in
5 515-520 PBSsurfactant
10 515-520 PBSsurfactant
15 520 PBSsurfactant
20 524 PBSsurfactant
30 526 PBSsurfactant
40 530 PBSsurfactant
50 535 PBSsurfactant
60 540 PBSsurfactant
80 553 PBSsurfactant
100 572 PBSsurfactant
17
18
GNP Advantage
- Color size dependent
- No toxicity
- DNA strong bonding
- Thiol covalent bond
19
The surface Hydrophobicity can be modified by
thiol compound
20
The surface self-assembling by thiol compound
21
GNP Application
Different bio sensor based self assemble design
22
23
The Sol-Gel Process is
a method for producing solid materials from small molecules
inn RT The method is used for the fabrication of metal oxides
especially the oxides of silicon and titanium
24
Alkoxide-sol-gel-method-preparation
25
HydrolysisCondensation
26
27
Why silica sol-gel coating
- Biocompatible
- Functionalization
- Physical trapping
- Chemical stability
- Physical stability
28
I Byun AW Coleman B Kim Micromech Microeng 23 (2013) 085016 29
Three Dimensional
Multi Dimensional matrix
One Dimensional
Layer by layer coatingSi
OR
ORRORO
Si
OR
RORO
NH2
Si
OR
RORO
SH
Si
OR
RORO O
O
n
Si
OR
RORO
HN
NH
NH2
Si
R
RRORO
30
Two Dimensional
Physical trapping in sol gel
31
32
apto ldquoto fitrdquo
mer ldquosmallest unit of repeating structurerdquo
What is an Aptamer
Oligonucleotide or Peptide
molecules that bind to a specific
target molecule
33
Types of Aptamer
- ssDNA
- dsDNA
- RNA
- Peptide
34
SELEX(Systematic Evolution of Ligands by Exponential Enrichment)
Selection of ligand sequences that bind to a target
partitioning of aptamers from
non-aptamers via affinity methods
amplification of bound aptamers
35
Targets of Aptamers
- Small Molecules
- Proteins
- Nucleic Acids
- Cells
- Tissues
- Organisms
lysozym Thrombin HIV TAR Hemin Interferon γ Vascular
endotherial growth factor (VEGF) Prostate specific antigen(PSA)
Dopamine hellip 36
37
MFMNP Design
Core Shell
Dumbbell Structure
Multicomponent Hybrid NP
38
39
Polymer Shell
Core Shell
Metal alloy
(FePt)
Magnetic nanolayer
(Fe3O4)
Thermal decomposition
of Fe(acac)3 on FePt
Magnetic NP
(Fe3O4)
Metal alloy
(Au Ag)
(Fe3O4) in Au3+ solution
at reduction condition
40
1 Core Shell structure
41
- Mix of NP during the growth of nanocrystals
- Sol-gel process
Core Shell synthesis
2 Dumbbell structures
42
- Mix of NP during the growth of nanocrystals
- Thermal rearrangement
- self-assemble at a liquid-liquid interface
- Functional sol-gel matrix
3 Multicomponent Hybrid NPs
Self-assembly processes provide an approach to
fabricating multicomponent hybrid NPs with
integrated multifunctionality
43
- Surfactant polymers
- Sol-gel process
44
Poly(lactico-co-glycolicacid)
(PLGA)
Poly(L-lysine)-PEG-folate
(PLL-PEG-FOL)
By mechanical
stirring at RT
in CH3Cl
45
46
W Lu SR Arumugam D Senapati ACSNANO 2010 4(3) 1739ndash1749
GNP GNP
GNP
RNA Aptamer
Anti-HER2C-reb-2 antibody
GNPCTAB
47
Magnetic nanoparticle surface modification Glucose oxidasse
(GOx)
PEGGPS
APSMNP
48Z Lin et al Analytica Chimica Acta 818 (2014) 54ndash60
49
P Sun H Zhang C Liu Langmuir 2010 26(2) 1278ndash1284 50
Immuno-labeling using Fe3O4CdTe nanocomposites which are formed by
linking multiple TGA-stabilized CdTe QDs with the thiol-functionalized silica
coated iron oxide nanoparticles
23-dimercaptosuccinic
acids (DMSA)Dopamine
Mercaptoundecanoic acid
(MUA)
HS COOH
4 Small multifunctional molecules
Glutaraldehyde 51
C J Xu K M Xu H W Gu R K Zheng H Liu X X Zhang Z H Guo B Xu J Am Chem Soc 2004 126 9938
Dopamine
Conditions (a) NaOH tert-butyl dicarbonate dioxane H2O 24 hrs (b) BnBr K2CO3 DMF
RT 24 h (c) 10 CF3COOH CH2Cl2 rt 5 h (d) succinic anhydride pyridine RTt 3 h (e)
NHS DCC DMAP CHCl3 RTt 3 h
NTA
52
3-mercapto trimethoxysilane
(MPS)
3-amino trimethoxysilane
(APS)
5 Silane multifunctional monomers
Glycidoxypropyltrimethoxy-silane
(GPS)
53
54
Multifunctional magnetic nanoparticles
application
Bioseparation
Biodetection(Real time monitoring)
Targeted drug delivery
Magnetic fluid Hyperthermia therapy
55
1 Specific Bio-Separation
bull Magnetic properties
bull Targeting agent
Cell organism molecule
56
His-tagged protein purification as
the first step towards recombinant
protein purification
58
Lysing Binding
Nickel
affinity
nanoparicle
His tag
fusion protein
Washing Elusion
Digestion
Binding
Nickel
affinity
nanoparticle
Washing Target
Recombinant
Protein
1- Preparation of Fe3O4SiO2Layered Double Hydroxide CoreminusShell
Microspheres for Magnetic Separation of Proteins J Am ChemSoc (2012)
18 134(2) 1071-7
Ni2+
2- Magnetic Nanocomposite Spheres Decorated with NiO Nanoparticles for a
Magnetically Recyclable Protein Separation System J Kim Y Piao N Lee Y
Park I-H Lee J-H Lee Adv Mater (2010) 22 57ndash60
His-tagged protein purification
Purification of His6-lipase using Fe3O4AundashLAndashIDAndashCu2+ NPs with various
amounts of NPs (lane 1 01 mg lane 2 02 mg lane 3
63
Synthetic procedure for the coreshell structure spheres
64
glutaraldehyde
(GLH)
Reference[1] H W Gu Z M Yang J H Gao C K Chang B Xu J Am Chem Soc 2005 127 34
[2] A Hutten D Sudfeld I Ennen G Reiss K Wojczykowski P Jutzi J Magn Magn Mater 2005
293 93
[3] G S Chaubey C Barcena N Poudyal C B Rong J M Gao S Sun J P Liu J Am Chem
Soc 2007 129 7214
[4] C W Kim Y H Kim H G Cha D K Lee Y S Kang J NanosciNanotechnol 2006 6 3417
[5] Y W Jun J S Choi J Cheon Angew Chem Int Ed 2006 45 3414
[6] X Michalet F F Pinaud L A Bentolila J M Tsay S Doose J J Li G Sundaresan A M Wu
S S Gambhir S Weiss Science 2005 307 538
[7] M Hu J Y Chen Z Y Li L Au G V Hartland X D Li M Marquez Y N Xia Chem Soc
Rev 2006 35 1084
[8] M Vallet-Regi F Balas D Arcos Angew Chem Int Ed 2007 46 7548
[9] S T Selvan P K Patra C Y Ang J Y Ying Angew Chem Int Ed 2007 46 2448
[10] I S Lee N Lee J Park B H Kim Y W Yi T Kim T K Kim I H Lee S R Paik T
Hyeon J Am Chem Soc 2006 128 10658
[11] Y S Lin S H Wu Y Hung Y H Chou C Chang M L Lin C P Tsai C Y Mou Chem
Mater 2006 18 5170
[12] B H Wu H Zhang C Chen S C Lin N F Zheng Nano Res 2009 2 975
[13] J Kim J E Lee J Lee J H Yu B C Kim K An Y Hwang C Shin J G Park J Kim T
Hyeon J Am Chem Soc 2006 128 688
[14] S Santra H S Yang P H Holloway J T Stanley R A Mericle J Am Chem Soc 2005 127
1656
[15] D K Yi S T Selvan S S Lee G C Papaefthymiou D Kundaliya J Y Ying J Am Chem
Soc 2005 127 4990 65
[16] J H Gao B Zhang Y Gao Y Pan X X Zhang B Xu J Am Chem Soc 2007 129 11928 [17] J H Gao W Zhang P B Huang B Zhang X X Zhang B Xu J Am Chem Soc 2008 130 3710 [18] J H Gao G L Liang B Zhang Y Kuang X X Zhang B Xu J Am Chem Soc 2007 129 1428 [19] J H Gao G L Liang J S Cheung Y Pan Y Kuang F Zhao B Zhang X X Zhang E X Wu B Xu J Am Chem Soc 2008 130 11828 [20] A R Kortan R Hull R L Opila M G Bawendi M L Steigerwald P J Carroll L E Brus JAm Chem Soc 1990 112 1327 [21] X G Peng M C Schlamp A V Kadavanich A P Alivisatos J Am Chem Soc 1997 119 7019 [22] S Kim B Fisher H J Eisler M Bawendi J Am Chem Soc 2003 125 11466[23] H Zeng J Li Z L Wang J P Liu S Sun Nano Lett 2004 4 187 [24] H Zeng S Sun J Li Z L Wang J P Liu Appl Phys Lett 2004 85 792 [25] Z Xu Y Hou S Sun J Am Chem Soc 2007 129 8698 [26] Y H Lin N Kim J Q Wang C J Zhong J Phys Chem B 2005 109 21593 [27] C Wang H Daimon S Sun Nano Lett 2009 9 1493 [28] K W Kwon M Shim J Am Chem Soc 2005 127 10269 [29] H Yu M Chen P M Rice S X Wang R L White S Sun Nano Lett 2005 5 379 [30] J S Choi Y W Jun S I Yeon H C Kim J S Shin J Cheon J Am Chem Soc 2006 128 15982 [31] S H Choi H B Na Y I Park K An S G Kwon Y Jang M Park J Moon J S Son I CSong W K Moon T Hyeon J Am Chem Soc 2008 130 15573 [32] H W Gu R K Zheng X X Zhang B Xu J Am Chem Soc 2004 126 5664
66
4- Thermal decomposition
MNP synthesized by the thermal decomposition of Fe(acac)3 in PEG and in MPEG
Tian XH Wei F Wang TX Wang P Int J Nanomedicine 201271031-4111
5- Sol-gel synthesis
Porous or hollow iron oxide nanostructures have
been synthesized by sol gel process
12
Wrapndash BakendashPeel process
Reduction
MagnetiteSilicaMagnetite
Removal of
silica using
NaOH
β-FeOOH β-FeOOHSilicaHematiteSilica
Removal of
silica using
NaOH
Hematite
Silica
coating
Heat
treatment
13
6- Kirkendall process(Nano hollow structures)
The rate of oxygen diffusion towards the core is slower than
diffusion of iron outwards from the core leading to the formation
of hollow structures
Fe
Fe3O4 Fe3O4
Fe
Fe3O4
Amorphous Crystalline
1 Oxidation by
(CH3)3NO
2 Heating
gt200oC
14
15
Colloidal stability by citrate
Au Au
- ---- - -
- ---O O
OO O
O
O H
Lowis bond
16
Color is size dependent
Size(nm) Emission(nm) Suspension in
5 515-520 PBSsurfactant
10 515-520 PBSsurfactant
15 520 PBSsurfactant
20 524 PBSsurfactant
30 526 PBSsurfactant
40 530 PBSsurfactant
50 535 PBSsurfactant
60 540 PBSsurfactant
80 553 PBSsurfactant
100 572 PBSsurfactant
17
18
GNP Advantage
- Color size dependent
- No toxicity
- DNA strong bonding
- Thiol covalent bond
19
The surface Hydrophobicity can be modified by
thiol compound
20
The surface self-assembling by thiol compound
21
GNP Application
Different bio sensor based self assemble design
22
23
The Sol-Gel Process is
a method for producing solid materials from small molecules
inn RT The method is used for the fabrication of metal oxides
especially the oxides of silicon and titanium
24
Alkoxide-sol-gel-method-preparation
25
HydrolysisCondensation
26
27
Why silica sol-gel coating
- Biocompatible
- Functionalization
- Physical trapping
- Chemical stability
- Physical stability
28
I Byun AW Coleman B Kim Micromech Microeng 23 (2013) 085016 29
Three Dimensional
Multi Dimensional matrix
One Dimensional
Layer by layer coatingSi
OR
ORRORO
Si
OR
RORO
NH2
Si
OR
RORO
SH
Si
OR
RORO O
O
n
Si
OR
RORO
HN
NH
NH2
Si
R
RRORO
30
Two Dimensional
Physical trapping in sol gel
31
32
apto ldquoto fitrdquo
mer ldquosmallest unit of repeating structurerdquo
What is an Aptamer
Oligonucleotide or Peptide
molecules that bind to a specific
target molecule
33
Types of Aptamer
- ssDNA
- dsDNA
- RNA
- Peptide
34
SELEX(Systematic Evolution of Ligands by Exponential Enrichment)
Selection of ligand sequences that bind to a target
partitioning of aptamers from
non-aptamers via affinity methods
amplification of bound aptamers
35
Targets of Aptamers
- Small Molecules
- Proteins
- Nucleic Acids
- Cells
- Tissues
- Organisms
lysozym Thrombin HIV TAR Hemin Interferon γ Vascular
endotherial growth factor (VEGF) Prostate specific antigen(PSA)
Dopamine hellip 36
37
MFMNP Design
Core Shell
Dumbbell Structure
Multicomponent Hybrid NP
38
39
Polymer Shell
Core Shell
Metal alloy
(FePt)
Magnetic nanolayer
(Fe3O4)
Thermal decomposition
of Fe(acac)3 on FePt
Magnetic NP
(Fe3O4)
Metal alloy
(Au Ag)
(Fe3O4) in Au3+ solution
at reduction condition
40
1 Core Shell structure
41
- Mix of NP during the growth of nanocrystals
- Sol-gel process
Core Shell synthesis
2 Dumbbell structures
42
- Mix of NP during the growth of nanocrystals
- Thermal rearrangement
- self-assemble at a liquid-liquid interface
- Functional sol-gel matrix
3 Multicomponent Hybrid NPs
Self-assembly processes provide an approach to
fabricating multicomponent hybrid NPs with
integrated multifunctionality
43
- Surfactant polymers
- Sol-gel process
44
Poly(lactico-co-glycolicacid)
(PLGA)
Poly(L-lysine)-PEG-folate
(PLL-PEG-FOL)
By mechanical
stirring at RT
in CH3Cl
45
46
W Lu SR Arumugam D Senapati ACSNANO 2010 4(3) 1739ndash1749
GNP GNP
GNP
RNA Aptamer
Anti-HER2C-reb-2 antibody
GNPCTAB
47
Magnetic nanoparticle surface modification Glucose oxidasse
(GOx)
PEGGPS
APSMNP
48Z Lin et al Analytica Chimica Acta 818 (2014) 54ndash60
49
P Sun H Zhang C Liu Langmuir 2010 26(2) 1278ndash1284 50
Immuno-labeling using Fe3O4CdTe nanocomposites which are formed by
linking multiple TGA-stabilized CdTe QDs with the thiol-functionalized silica
coated iron oxide nanoparticles
23-dimercaptosuccinic
acids (DMSA)Dopamine
Mercaptoundecanoic acid
(MUA)
HS COOH
4 Small multifunctional molecules
Glutaraldehyde 51
C J Xu K M Xu H W Gu R K Zheng H Liu X X Zhang Z H Guo B Xu J Am Chem Soc 2004 126 9938
Dopamine
Conditions (a) NaOH tert-butyl dicarbonate dioxane H2O 24 hrs (b) BnBr K2CO3 DMF
RT 24 h (c) 10 CF3COOH CH2Cl2 rt 5 h (d) succinic anhydride pyridine RTt 3 h (e)
NHS DCC DMAP CHCl3 RTt 3 h
NTA
52
3-mercapto trimethoxysilane
(MPS)
3-amino trimethoxysilane
(APS)
5 Silane multifunctional monomers
Glycidoxypropyltrimethoxy-silane
(GPS)
53
54
Multifunctional magnetic nanoparticles
application
Bioseparation
Biodetection(Real time monitoring)
Targeted drug delivery
Magnetic fluid Hyperthermia therapy
55
1 Specific Bio-Separation
bull Magnetic properties
bull Targeting agent
Cell organism molecule
56
His-tagged protein purification as
the first step towards recombinant
protein purification
58
Lysing Binding
Nickel
affinity
nanoparicle
His tag
fusion protein
Washing Elusion
Digestion
Binding
Nickel
affinity
nanoparticle
Washing Target
Recombinant
Protein
1- Preparation of Fe3O4SiO2Layered Double Hydroxide CoreminusShell
Microspheres for Magnetic Separation of Proteins J Am ChemSoc (2012)
18 134(2) 1071-7
Ni2+
2- Magnetic Nanocomposite Spheres Decorated with NiO Nanoparticles for a
Magnetically Recyclable Protein Separation System J Kim Y Piao N Lee Y
Park I-H Lee J-H Lee Adv Mater (2010) 22 57ndash60
His-tagged protein purification
Purification of His6-lipase using Fe3O4AundashLAndashIDAndashCu2+ NPs with various
amounts of NPs (lane 1 01 mg lane 2 02 mg lane 3
63
Synthetic procedure for the coreshell structure spheres
64
glutaraldehyde
(GLH)
Reference[1] H W Gu Z M Yang J H Gao C K Chang B Xu J Am Chem Soc 2005 127 34
[2] A Hutten D Sudfeld I Ennen G Reiss K Wojczykowski P Jutzi J Magn Magn Mater 2005
293 93
[3] G S Chaubey C Barcena N Poudyal C B Rong J M Gao S Sun J P Liu J Am Chem
Soc 2007 129 7214
[4] C W Kim Y H Kim H G Cha D K Lee Y S Kang J NanosciNanotechnol 2006 6 3417
[5] Y W Jun J S Choi J Cheon Angew Chem Int Ed 2006 45 3414
[6] X Michalet F F Pinaud L A Bentolila J M Tsay S Doose J J Li G Sundaresan A M Wu
S S Gambhir S Weiss Science 2005 307 538
[7] M Hu J Y Chen Z Y Li L Au G V Hartland X D Li M Marquez Y N Xia Chem Soc
Rev 2006 35 1084
[8] M Vallet-Regi F Balas D Arcos Angew Chem Int Ed 2007 46 7548
[9] S T Selvan P K Patra C Y Ang J Y Ying Angew Chem Int Ed 2007 46 2448
[10] I S Lee N Lee J Park B H Kim Y W Yi T Kim T K Kim I H Lee S R Paik T
Hyeon J Am Chem Soc 2006 128 10658
[11] Y S Lin S H Wu Y Hung Y H Chou C Chang M L Lin C P Tsai C Y Mou Chem
Mater 2006 18 5170
[12] B H Wu H Zhang C Chen S C Lin N F Zheng Nano Res 2009 2 975
[13] J Kim J E Lee J Lee J H Yu B C Kim K An Y Hwang C Shin J G Park J Kim T
Hyeon J Am Chem Soc 2006 128 688
[14] S Santra H S Yang P H Holloway J T Stanley R A Mericle J Am Chem Soc 2005 127
1656
[15] D K Yi S T Selvan S S Lee G C Papaefthymiou D Kundaliya J Y Ying J Am Chem
Soc 2005 127 4990 65
[16] J H Gao B Zhang Y Gao Y Pan X X Zhang B Xu J Am Chem Soc 2007 129 11928 [17] J H Gao W Zhang P B Huang B Zhang X X Zhang B Xu J Am Chem Soc 2008 130 3710 [18] J H Gao G L Liang B Zhang Y Kuang X X Zhang B Xu J Am Chem Soc 2007 129 1428 [19] J H Gao G L Liang J S Cheung Y Pan Y Kuang F Zhao B Zhang X X Zhang E X Wu B Xu J Am Chem Soc 2008 130 11828 [20] A R Kortan R Hull R L Opila M G Bawendi M L Steigerwald P J Carroll L E Brus JAm Chem Soc 1990 112 1327 [21] X G Peng M C Schlamp A V Kadavanich A P Alivisatos J Am Chem Soc 1997 119 7019 [22] S Kim B Fisher H J Eisler M Bawendi J Am Chem Soc 2003 125 11466[23] H Zeng J Li Z L Wang J P Liu S Sun Nano Lett 2004 4 187 [24] H Zeng S Sun J Li Z L Wang J P Liu Appl Phys Lett 2004 85 792 [25] Z Xu Y Hou S Sun J Am Chem Soc 2007 129 8698 [26] Y H Lin N Kim J Q Wang C J Zhong J Phys Chem B 2005 109 21593 [27] C Wang H Daimon S Sun Nano Lett 2009 9 1493 [28] K W Kwon M Shim J Am Chem Soc 2005 127 10269 [29] H Yu M Chen P M Rice S X Wang R L White S Sun Nano Lett 2005 5 379 [30] J S Choi Y W Jun S I Yeon H C Kim J S Shin J Cheon J Am Chem Soc 2006 128 15982 [31] S H Choi H B Na Y I Park K An S G Kwon Y Jang M Park J Moon J S Son I CSong W K Moon T Hyeon J Am Chem Soc 2008 130 15573 [32] H W Gu R K Zheng X X Zhang B Xu J Am Chem Soc 2004 126 5664
66
5- Sol-gel synthesis
Porous or hollow iron oxide nanostructures have
been synthesized by sol gel process
12
Wrapndash BakendashPeel process
Reduction
MagnetiteSilicaMagnetite
Removal of
silica using
NaOH
β-FeOOH β-FeOOHSilicaHematiteSilica
Removal of
silica using
NaOH
Hematite
Silica
coating
Heat
treatment
13
6- Kirkendall process(Nano hollow structures)
The rate of oxygen diffusion towards the core is slower than
diffusion of iron outwards from the core leading to the formation
of hollow structures
Fe
Fe3O4 Fe3O4
Fe
Fe3O4
Amorphous Crystalline
1 Oxidation by
(CH3)3NO
2 Heating
gt200oC
14
15
Colloidal stability by citrate
Au Au
- ---- - -
- ---O O
OO O
O
O H
Lowis bond
16
Color is size dependent
Size(nm) Emission(nm) Suspension in
5 515-520 PBSsurfactant
10 515-520 PBSsurfactant
15 520 PBSsurfactant
20 524 PBSsurfactant
30 526 PBSsurfactant
40 530 PBSsurfactant
50 535 PBSsurfactant
60 540 PBSsurfactant
80 553 PBSsurfactant
100 572 PBSsurfactant
17
18
GNP Advantage
- Color size dependent
- No toxicity
- DNA strong bonding
- Thiol covalent bond
19
The surface Hydrophobicity can be modified by
thiol compound
20
The surface self-assembling by thiol compound
21
GNP Application
Different bio sensor based self assemble design
22
23
The Sol-Gel Process is
a method for producing solid materials from small molecules
inn RT The method is used for the fabrication of metal oxides
especially the oxides of silicon and titanium
24
Alkoxide-sol-gel-method-preparation
25
HydrolysisCondensation
26
27
Why silica sol-gel coating
- Biocompatible
- Functionalization
- Physical trapping
- Chemical stability
- Physical stability
28
I Byun AW Coleman B Kim Micromech Microeng 23 (2013) 085016 29
Three Dimensional
Multi Dimensional matrix
One Dimensional
Layer by layer coatingSi
OR
ORRORO
Si
OR
RORO
NH2
Si
OR
RORO
SH
Si
OR
RORO O
O
n
Si
OR
RORO
HN
NH
NH2
Si
R
RRORO
30
Two Dimensional
Physical trapping in sol gel
31
32
apto ldquoto fitrdquo
mer ldquosmallest unit of repeating structurerdquo
What is an Aptamer
Oligonucleotide or Peptide
molecules that bind to a specific
target molecule
33
Types of Aptamer
- ssDNA
- dsDNA
- RNA
- Peptide
34
SELEX(Systematic Evolution of Ligands by Exponential Enrichment)
Selection of ligand sequences that bind to a target
partitioning of aptamers from
non-aptamers via affinity methods
amplification of bound aptamers
35
Targets of Aptamers
- Small Molecules
- Proteins
- Nucleic Acids
- Cells
- Tissues
- Organisms
lysozym Thrombin HIV TAR Hemin Interferon γ Vascular
endotherial growth factor (VEGF) Prostate specific antigen(PSA)
Dopamine hellip 36
37
MFMNP Design
Core Shell
Dumbbell Structure
Multicomponent Hybrid NP
38
39
Polymer Shell
Core Shell
Metal alloy
(FePt)
Magnetic nanolayer
(Fe3O4)
Thermal decomposition
of Fe(acac)3 on FePt
Magnetic NP
(Fe3O4)
Metal alloy
(Au Ag)
(Fe3O4) in Au3+ solution
at reduction condition
40
1 Core Shell structure
41
- Mix of NP during the growth of nanocrystals
- Sol-gel process
Core Shell synthesis
2 Dumbbell structures
42
- Mix of NP during the growth of nanocrystals
- Thermal rearrangement
- self-assemble at a liquid-liquid interface
- Functional sol-gel matrix
3 Multicomponent Hybrid NPs
Self-assembly processes provide an approach to
fabricating multicomponent hybrid NPs with
integrated multifunctionality
43
- Surfactant polymers
- Sol-gel process
44
Poly(lactico-co-glycolicacid)
(PLGA)
Poly(L-lysine)-PEG-folate
(PLL-PEG-FOL)
By mechanical
stirring at RT
in CH3Cl
45
46
W Lu SR Arumugam D Senapati ACSNANO 2010 4(3) 1739ndash1749
GNP GNP
GNP
RNA Aptamer
Anti-HER2C-reb-2 antibody
GNPCTAB
47
Magnetic nanoparticle surface modification Glucose oxidasse
(GOx)
PEGGPS
APSMNP
48Z Lin et al Analytica Chimica Acta 818 (2014) 54ndash60
49
P Sun H Zhang C Liu Langmuir 2010 26(2) 1278ndash1284 50
Immuno-labeling using Fe3O4CdTe nanocomposites which are formed by
linking multiple TGA-stabilized CdTe QDs with the thiol-functionalized silica
coated iron oxide nanoparticles
23-dimercaptosuccinic
acids (DMSA)Dopamine
Mercaptoundecanoic acid
(MUA)
HS COOH
4 Small multifunctional molecules
Glutaraldehyde 51
C J Xu K M Xu H W Gu R K Zheng H Liu X X Zhang Z H Guo B Xu J Am Chem Soc 2004 126 9938
Dopamine
Conditions (a) NaOH tert-butyl dicarbonate dioxane H2O 24 hrs (b) BnBr K2CO3 DMF
RT 24 h (c) 10 CF3COOH CH2Cl2 rt 5 h (d) succinic anhydride pyridine RTt 3 h (e)
NHS DCC DMAP CHCl3 RTt 3 h
NTA
52
3-mercapto trimethoxysilane
(MPS)
3-amino trimethoxysilane
(APS)
5 Silane multifunctional monomers
Glycidoxypropyltrimethoxy-silane
(GPS)
53
54
Multifunctional magnetic nanoparticles
application
Bioseparation
Biodetection(Real time monitoring)
Targeted drug delivery
Magnetic fluid Hyperthermia therapy
55
1 Specific Bio-Separation
bull Magnetic properties
bull Targeting agent
Cell organism molecule
56
His-tagged protein purification as
the first step towards recombinant
protein purification
58
Lysing Binding
Nickel
affinity
nanoparicle
His tag
fusion protein
Washing Elusion
Digestion
Binding
Nickel
affinity
nanoparticle
Washing Target
Recombinant
Protein
1- Preparation of Fe3O4SiO2Layered Double Hydroxide CoreminusShell
Microspheres for Magnetic Separation of Proteins J Am ChemSoc (2012)
18 134(2) 1071-7
Ni2+
2- Magnetic Nanocomposite Spheres Decorated with NiO Nanoparticles for a
Magnetically Recyclable Protein Separation System J Kim Y Piao N Lee Y
Park I-H Lee J-H Lee Adv Mater (2010) 22 57ndash60
His-tagged protein purification
Purification of His6-lipase using Fe3O4AundashLAndashIDAndashCu2+ NPs with various
amounts of NPs (lane 1 01 mg lane 2 02 mg lane 3
63
Synthetic procedure for the coreshell structure spheres
64
glutaraldehyde
(GLH)
Reference[1] H W Gu Z M Yang J H Gao C K Chang B Xu J Am Chem Soc 2005 127 34
[2] A Hutten D Sudfeld I Ennen G Reiss K Wojczykowski P Jutzi J Magn Magn Mater 2005
293 93
[3] G S Chaubey C Barcena N Poudyal C B Rong J M Gao S Sun J P Liu J Am Chem
Soc 2007 129 7214
[4] C W Kim Y H Kim H G Cha D K Lee Y S Kang J NanosciNanotechnol 2006 6 3417
[5] Y W Jun J S Choi J Cheon Angew Chem Int Ed 2006 45 3414
[6] X Michalet F F Pinaud L A Bentolila J M Tsay S Doose J J Li G Sundaresan A M Wu
S S Gambhir S Weiss Science 2005 307 538
[7] M Hu J Y Chen Z Y Li L Au G V Hartland X D Li M Marquez Y N Xia Chem Soc
Rev 2006 35 1084
[8] M Vallet-Regi F Balas D Arcos Angew Chem Int Ed 2007 46 7548
[9] S T Selvan P K Patra C Y Ang J Y Ying Angew Chem Int Ed 2007 46 2448
[10] I S Lee N Lee J Park B H Kim Y W Yi T Kim T K Kim I H Lee S R Paik T
Hyeon J Am Chem Soc 2006 128 10658
[11] Y S Lin S H Wu Y Hung Y H Chou C Chang M L Lin C P Tsai C Y Mou Chem
Mater 2006 18 5170
[12] B H Wu H Zhang C Chen S C Lin N F Zheng Nano Res 2009 2 975
[13] J Kim J E Lee J Lee J H Yu B C Kim K An Y Hwang C Shin J G Park J Kim T
Hyeon J Am Chem Soc 2006 128 688
[14] S Santra H S Yang P H Holloway J T Stanley R A Mericle J Am Chem Soc 2005 127
1656
[15] D K Yi S T Selvan S S Lee G C Papaefthymiou D Kundaliya J Y Ying J Am Chem
Soc 2005 127 4990 65
[16] J H Gao B Zhang Y Gao Y Pan X X Zhang B Xu J Am Chem Soc 2007 129 11928 [17] J H Gao W Zhang P B Huang B Zhang X X Zhang B Xu J Am Chem Soc 2008 130 3710 [18] J H Gao G L Liang B Zhang Y Kuang X X Zhang B Xu J Am Chem Soc 2007 129 1428 [19] J H Gao G L Liang J S Cheung Y Pan Y Kuang F Zhao B Zhang X X Zhang E X Wu B Xu J Am Chem Soc 2008 130 11828 [20] A R Kortan R Hull R L Opila M G Bawendi M L Steigerwald P J Carroll L E Brus JAm Chem Soc 1990 112 1327 [21] X G Peng M C Schlamp A V Kadavanich A P Alivisatos J Am Chem Soc 1997 119 7019 [22] S Kim B Fisher H J Eisler M Bawendi J Am Chem Soc 2003 125 11466[23] H Zeng J Li Z L Wang J P Liu S Sun Nano Lett 2004 4 187 [24] H Zeng S Sun J Li Z L Wang J P Liu Appl Phys Lett 2004 85 792 [25] Z Xu Y Hou S Sun J Am Chem Soc 2007 129 8698 [26] Y H Lin N Kim J Q Wang C J Zhong J Phys Chem B 2005 109 21593 [27] C Wang H Daimon S Sun Nano Lett 2009 9 1493 [28] K W Kwon M Shim J Am Chem Soc 2005 127 10269 [29] H Yu M Chen P M Rice S X Wang R L White S Sun Nano Lett 2005 5 379 [30] J S Choi Y W Jun S I Yeon H C Kim J S Shin J Cheon J Am Chem Soc 2006 128 15982 [31] S H Choi H B Na Y I Park K An S G Kwon Y Jang M Park J Moon J S Son I CSong W K Moon T Hyeon J Am Chem Soc 2008 130 15573 [32] H W Gu R K Zheng X X Zhang B Xu J Am Chem Soc 2004 126 5664
66
Wrapndash BakendashPeel process
Reduction
MagnetiteSilicaMagnetite
Removal of
silica using
NaOH
β-FeOOH β-FeOOHSilicaHematiteSilica
Removal of
silica using
NaOH
Hematite
Silica
coating
Heat
treatment
13
6- Kirkendall process(Nano hollow structures)
The rate of oxygen diffusion towards the core is slower than
diffusion of iron outwards from the core leading to the formation
of hollow structures
Fe
Fe3O4 Fe3O4
Fe
Fe3O4
Amorphous Crystalline
1 Oxidation by
(CH3)3NO
2 Heating
gt200oC
14
15
Colloidal stability by citrate
Au Au
- ---- - -
- ---O O
OO O
O
O H
Lowis bond
16
Color is size dependent
Size(nm) Emission(nm) Suspension in
5 515-520 PBSsurfactant
10 515-520 PBSsurfactant
15 520 PBSsurfactant
20 524 PBSsurfactant
30 526 PBSsurfactant
40 530 PBSsurfactant
50 535 PBSsurfactant
60 540 PBSsurfactant
80 553 PBSsurfactant
100 572 PBSsurfactant
17
18
GNP Advantage
- Color size dependent
- No toxicity
- DNA strong bonding
- Thiol covalent bond
19
The surface Hydrophobicity can be modified by
thiol compound
20
The surface self-assembling by thiol compound
21
GNP Application
Different bio sensor based self assemble design
22
23
The Sol-Gel Process is
a method for producing solid materials from small molecules
inn RT The method is used for the fabrication of metal oxides
especially the oxides of silicon and titanium
24
Alkoxide-sol-gel-method-preparation
25
HydrolysisCondensation
26
27
Why silica sol-gel coating
- Biocompatible
- Functionalization
- Physical trapping
- Chemical stability
- Physical stability
28
I Byun AW Coleman B Kim Micromech Microeng 23 (2013) 085016 29
Three Dimensional
Multi Dimensional matrix
One Dimensional
Layer by layer coatingSi
OR
ORRORO
Si
OR
RORO
NH2
Si
OR
RORO
SH
Si
OR
RORO O
O
n
Si
OR
RORO
HN
NH
NH2
Si
R
RRORO
30
Two Dimensional
Physical trapping in sol gel
31
32
apto ldquoto fitrdquo
mer ldquosmallest unit of repeating structurerdquo
What is an Aptamer
Oligonucleotide or Peptide
molecules that bind to a specific
target molecule
33
Types of Aptamer
- ssDNA
- dsDNA
- RNA
- Peptide
34
SELEX(Systematic Evolution of Ligands by Exponential Enrichment)
Selection of ligand sequences that bind to a target
partitioning of aptamers from
non-aptamers via affinity methods
amplification of bound aptamers
35
Targets of Aptamers
- Small Molecules
- Proteins
- Nucleic Acids
- Cells
- Tissues
- Organisms
lysozym Thrombin HIV TAR Hemin Interferon γ Vascular
endotherial growth factor (VEGF) Prostate specific antigen(PSA)
Dopamine hellip 36
37
MFMNP Design
Core Shell
Dumbbell Structure
Multicomponent Hybrid NP
38
39
Polymer Shell
Core Shell
Metal alloy
(FePt)
Magnetic nanolayer
(Fe3O4)
Thermal decomposition
of Fe(acac)3 on FePt
Magnetic NP
(Fe3O4)
Metal alloy
(Au Ag)
(Fe3O4) in Au3+ solution
at reduction condition
40
1 Core Shell structure
41
- Mix of NP during the growth of nanocrystals
- Sol-gel process
Core Shell synthesis
2 Dumbbell structures
42
- Mix of NP during the growth of nanocrystals
- Thermal rearrangement
- self-assemble at a liquid-liquid interface
- Functional sol-gel matrix
3 Multicomponent Hybrid NPs
Self-assembly processes provide an approach to
fabricating multicomponent hybrid NPs with
integrated multifunctionality
43
- Surfactant polymers
- Sol-gel process
44
Poly(lactico-co-glycolicacid)
(PLGA)
Poly(L-lysine)-PEG-folate
(PLL-PEG-FOL)
By mechanical
stirring at RT
in CH3Cl
45
46
W Lu SR Arumugam D Senapati ACSNANO 2010 4(3) 1739ndash1749
GNP GNP
GNP
RNA Aptamer
Anti-HER2C-reb-2 antibody
GNPCTAB
47
Magnetic nanoparticle surface modification Glucose oxidasse
(GOx)
PEGGPS
APSMNP
48Z Lin et al Analytica Chimica Acta 818 (2014) 54ndash60
49
P Sun H Zhang C Liu Langmuir 2010 26(2) 1278ndash1284 50
Immuno-labeling using Fe3O4CdTe nanocomposites which are formed by
linking multiple TGA-stabilized CdTe QDs with the thiol-functionalized silica
coated iron oxide nanoparticles
23-dimercaptosuccinic
acids (DMSA)Dopamine
Mercaptoundecanoic acid
(MUA)
HS COOH
4 Small multifunctional molecules
Glutaraldehyde 51
C J Xu K M Xu H W Gu R K Zheng H Liu X X Zhang Z H Guo B Xu J Am Chem Soc 2004 126 9938
Dopamine
Conditions (a) NaOH tert-butyl dicarbonate dioxane H2O 24 hrs (b) BnBr K2CO3 DMF
RT 24 h (c) 10 CF3COOH CH2Cl2 rt 5 h (d) succinic anhydride pyridine RTt 3 h (e)
NHS DCC DMAP CHCl3 RTt 3 h
NTA
52
3-mercapto trimethoxysilane
(MPS)
3-amino trimethoxysilane
(APS)
5 Silane multifunctional monomers
Glycidoxypropyltrimethoxy-silane
(GPS)
53
54
Multifunctional magnetic nanoparticles
application
Bioseparation
Biodetection(Real time monitoring)
Targeted drug delivery
Magnetic fluid Hyperthermia therapy
55
1 Specific Bio-Separation
bull Magnetic properties
bull Targeting agent
Cell organism molecule
56
His-tagged protein purification as
the first step towards recombinant
protein purification
58
Lysing Binding
Nickel
affinity
nanoparicle
His tag
fusion protein
Washing Elusion
Digestion
Binding
Nickel
affinity
nanoparticle
Washing Target
Recombinant
Protein
1- Preparation of Fe3O4SiO2Layered Double Hydroxide CoreminusShell
Microspheres for Magnetic Separation of Proteins J Am ChemSoc (2012)
18 134(2) 1071-7
Ni2+
2- Magnetic Nanocomposite Spheres Decorated with NiO Nanoparticles for a
Magnetically Recyclable Protein Separation System J Kim Y Piao N Lee Y
Park I-H Lee J-H Lee Adv Mater (2010) 22 57ndash60
His-tagged protein purification
Purification of His6-lipase using Fe3O4AundashLAndashIDAndashCu2+ NPs with various
amounts of NPs (lane 1 01 mg lane 2 02 mg lane 3
63
Synthetic procedure for the coreshell structure spheres
64
glutaraldehyde
(GLH)
Reference[1] H W Gu Z M Yang J H Gao C K Chang B Xu J Am Chem Soc 2005 127 34
[2] A Hutten D Sudfeld I Ennen G Reiss K Wojczykowski P Jutzi J Magn Magn Mater 2005
293 93
[3] G S Chaubey C Barcena N Poudyal C B Rong J M Gao S Sun J P Liu J Am Chem
Soc 2007 129 7214
[4] C W Kim Y H Kim H G Cha D K Lee Y S Kang J NanosciNanotechnol 2006 6 3417
[5] Y W Jun J S Choi J Cheon Angew Chem Int Ed 2006 45 3414
[6] X Michalet F F Pinaud L A Bentolila J M Tsay S Doose J J Li G Sundaresan A M Wu
S S Gambhir S Weiss Science 2005 307 538
[7] M Hu J Y Chen Z Y Li L Au G V Hartland X D Li M Marquez Y N Xia Chem Soc
Rev 2006 35 1084
[8] M Vallet-Regi F Balas D Arcos Angew Chem Int Ed 2007 46 7548
[9] S T Selvan P K Patra C Y Ang J Y Ying Angew Chem Int Ed 2007 46 2448
[10] I S Lee N Lee J Park B H Kim Y W Yi T Kim T K Kim I H Lee S R Paik T
Hyeon J Am Chem Soc 2006 128 10658
[11] Y S Lin S H Wu Y Hung Y H Chou C Chang M L Lin C P Tsai C Y Mou Chem
Mater 2006 18 5170
[12] B H Wu H Zhang C Chen S C Lin N F Zheng Nano Res 2009 2 975
[13] J Kim J E Lee J Lee J H Yu B C Kim K An Y Hwang C Shin J G Park J Kim T
Hyeon J Am Chem Soc 2006 128 688
[14] S Santra H S Yang P H Holloway J T Stanley R A Mericle J Am Chem Soc 2005 127
1656
[15] D K Yi S T Selvan S S Lee G C Papaefthymiou D Kundaliya J Y Ying J Am Chem
Soc 2005 127 4990 65
[16] J H Gao B Zhang Y Gao Y Pan X X Zhang B Xu J Am Chem Soc 2007 129 11928 [17] J H Gao W Zhang P B Huang B Zhang X X Zhang B Xu J Am Chem Soc 2008 130 3710 [18] J H Gao G L Liang B Zhang Y Kuang X X Zhang B Xu J Am Chem Soc 2007 129 1428 [19] J H Gao G L Liang J S Cheung Y Pan Y Kuang F Zhao B Zhang X X Zhang E X Wu B Xu J Am Chem Soc 2008 130 11828 [20] A R Kortan R Hull R L Opila M G Bawendi M L Steigerwald P J Carroll L E Brus JAm Chem Soc 1990 112 1327 [21] X G Peng M C Schlamp A V Kadavanich A P Alivisatos J Am Chem Soc 1997 119 7019 [22] S Kim B Fisher H J Eisler M Bawendi J Am Chem Soc 2003 125 11466[23] H Zeng J Li Z L Wang J P Liu S Sun Nano Lett 2004 4 187 [24] H Zeng S Sun J Li Z L Wang J P Liu Appl Phys Lett 2004 85 792 [25] Z Xu Y Hou S Sun J Am Chem Soc 2007 129 8698 [26] Y H Lin N Kim J Q Wang C J Zhong J Phys Chem B 2005 109 21593 [27] C Wang H Daimon S Sun Nano Lett 2009 9 1493 [28] K W Kwon M Shim J Am Chem Soc 2005 127 10269 [29] H Yu M Chen P M Rice S X Wang R L White S Sun Nano Lett 2005 5 379 [30] J S Choi Y W Jun S I Yeon H C Kim J S Shin J Cheon J Am Chem Soc 2006 128 15982 [31] S H Choi H B Na Y I Park K An S G Kwon Y Jang M Park J Moon J S Son I CSong W K Moon T Hyeon J Am Chem Soc 2008 130 15573 [32] H W Gu R K Zheng X X Zhang B Xu J Am Chem Soc 2004 126 5664
66
6- Kirkendall process(Nano hollow structures)
The rate of oxygen diffusion towards the core is slower than
diffusion of iron outwards from the core leading to the formation
of hollow structures
Fe
Fe3O4 Fe3O4
Fe
Fe3O4
Amorphous Crystalline
1 Oxidation by
(CH3)3NO
2 Heating
gt200oC
14
15
Colloidal stability by citrate
Au Au
- ---- - -
- ---O O
OO O
O
O H
Lowis bond
16
Color is size dependent
Size(nm) Emission(nm) Suspension in
5 515-520 PBSsurfactant
10 515-520 PBSsurfactant
15 520 PBSsurfactant
20 524 PBSsurfactant
30 526 PBSsurfactant
40 530 PBSsurfactant
50 535 PBSsurfactant
60 540 PBSsurfactant
80 553 PBSsurfactant
100 572 PBSsurfactant
17
18
GNP Advantage
- Color size dependent
- No toxicity
- DNA strong bonding
- Thiol covalent bond
19
The surface Hydrophobicity can be modified by
thiol compound
20
The surface self-assembling by thiol compound
21
GNP Application
Different bio sensor based self assemble design
22
23
The Sol-Gel Process is
a method for producing solid materials from small molecules
inn RT The method is used for the fabrication of metal oxides
especially the oxides of silicon and titanium
24
Alkoxide-sol-gel-method-preparation
25
HydrolysisCondensation
26
27
Why silica sol-gel coating
- Biocompatible
- Functionalization
- Physical trapping
- Chemical stability
- Physical stability
28
I Byun AW Coleman B Kim Micromech Microeng 23 (2013) 085016 29
Three Dimensional
Multi Dimensional matrix
One Dimensional
Layer by layer coatingSi
OR
ORRORO
Si
OR
RORO
NH2
Si
OR
RORO
SH
Si
OR
RORO O
O
n
Si
OR
RORO
HN
NH
NH2
Si
R
RRORO
30
Two Dimensional
Physical trapping in sol gel
31
32
apto ldquoto fitrdquo
mer ldquosmallest unit of repeating structurerdquo
What is an Aptamer
Oligonucleotide or Peptide
molecules that bind to a specific
target molecule
33
Types of Aptamer
- ssDNA
- dsDNA
- RNA
- Peptide
34
SELEX(Systematic Evolution of Ligands by Exponential Enrichment)
Selection of ligand sequences that bind to a target
partitioning of aptamers from
non-aptamers via affinity methods
amplification of bound aptamers
35
Targets of Aptamers
- Small Molecules
- Proteins
- Nucleic Acids
- Cells
- Tissues
- Organisms
lysozym Thrombin HIV TAR Hemin Interferon γ Vascular
endotherial growth factor (VEGF) Prostate specific antigen(PSA)
Dopamine hellip 36
37
MFMNP Design
Core Shell
Dumbbell Structure
Multicomponent Hybrid NP
38
39
Polymer Shell
Core Shell
Metal alloy
(FePt)
Magnetic nanolayer
(Fe3O4)
Thermal decomposition
of Fe(acac)3 on FePt
Magnetic NP
(Fe3O4)
Metal alloy
(Au Ag)
(Fe3O4) in Au3+ solution
at reduction condition
40
1 Core Shell structure
41
- Mix of NP during the growth of nanocrystals
- Sol-gel process
Core Shell synthesis
2 Dumbbell structures
42
- Mix of NP during the growth of nanocrystals
- Thermal rearrangement
- self-assemble at a liquid-liquid interface
- Functional sol-gel matrix
3 Multicomponent Hybrid NPs
Self-assembly processes provide an approach to
fabricating multicomponent hybrid NPs with
integrated multifunctionality
43
- Surfactant polymers
- Sol-gel process
44
Poly(lactico-co-glycolicacid)
(PLGA)
Poly(L-lysine)-PEG-folate
(PLL-PEG-FOL)
By mechanical
stirring at RT
in CH3Cl
45
46
W Lu SR Arumugam D Senapati ACSNANO 2010 4(3) 1739ndash1749
GNP GNP
GNP
RNA Aptamer
Anti-HER2C-reb-2 antibody
GNPCTAB
47
Magnetic nanoparticle surface modification Glucose oxidasse
(GOx)
PEGGPS
APSMNP
48Z Lin et al Analytica Chimica Acta 818 (2014) 54ndash60
49
P Sun H Zhang C Liu Langmuir 2010 26(2) 1278ndash1284 50
Immuno-labeling using Fe3O4CdTe nanocomposites which are formed by
linking multiple TGA-stabilized CdTe QDs with the thiol-functionalized silica
coated iron oxide nanoparticles
23-dimercaptosuccinic
acids (DMSA)Dopamine
Mercaptoundecanoic acid
(MUA)
HS COOH
4 Small multifunctional molecules
Glutaraldehyde 51
C J Xu K M Xu H W Gu R K Zheng H Liu X X Zhang Z H Guo B Xu J Am Chem Soc 2004 126 9938
Dopamine
Conditions (a) NaOH tert-butyl dicarbonate dioxane H2O 24 hrs (b) BnBr K2CO3 DMF
RT 24 h (c) 10 CF3COOH CH2Cl2 rt 5 h (d) succinic anhydride pyridine RTt 3 h (e)
NHS DCC DMAP CHCl3 RTt 3 h
NTA
52
3-mercapto trimethoxysilane
(MPS)
3-amino trimethoxysilane
(APS)
5 Silane multifunctional monomers
Glycidoxypropyltrimethoxy-silane
(GPS)
53
54
Multifunctional magnetic nanoparticles
application
Bioseparation
Biodetection(Real time monitoring)
Targeted drug delivery
Magnetic fluid Hyperthermia therapy
55
1 Specific Bio-Separation
bull Magnetic properties
bull Targeting agent
Cell organism molecule
56
His-tagged protein purification as
the first step towards recombinant
protein purification
58
Lysing Binding
Nickel
affinity
nanoparicle
His tag
fusion protein
Washing Elusion
Digestion
Binding
Nickel
affinity
nanoparticle
Washing Target
Recombinant
Protein
1- Preparation of Fe3O4SiO2Layered Double Hydroxide CoreminusShell
Microspheres for Magnetic Separation of Proteins J Am ChemSoc (2012)
18 134(2) 1071-7
Ni2+
2- Magnetic Nanocomposite Spheres Decorated with NiO Nanoparticles for a
Magnetically Recyclable Protein Separation System J Kim Y Piao N Lee Y
Park I-H Lee J-H Lee Adv Mater (2010) 22 57ndash60
His-tagged protein purification
Purification of His6-lipase using Fe3O4AundashLAndashIDAndashCu2+ NPs with various
amounts of NPs (lane 1 01 mg lane 2 02 mg lane 3
63
Synthetic procedure for the coreshell structure spheres
64
glutaraldehyde
(GLH)
Reference[1] H W Gu Z M Yang J H Gao C K Chang B Xu J Am Chem Soc 2005 127 34
[2] A Hutten D Sudfeld I Ennen G Reiss K Wojczykowski P Jutzi J Magn Magn Mater 2005
293 93
[3] G S Chaubey C Barcena N Poudyal C B Rong J M Gao S Sun J P Liu J Am Chem
Soc 2007 129 7214
[4] C W Kim Y H Kim H G Cha D K Lee Y S Kang J NanosciNanotechnol 2006 6 3417
[5] Y W Jun J S Choi J Cheon Angew Chem Int Ed 2006 45 3414
[6] X Michalet F F Pinaud L A Bentolila J M Tsay S Doose J J Li G Sundaresan A M Wu
S S Gambhir S Weiss Science 2005 307 538
[7] M Hu J Y Chen Z Y Li L Au G V Hartland X D Li M Marquez Y N Xia Chem Soc
Rev 2006 35 1084
[8] M Vallet-Regi F Balas D Arcos Angew Chem Int Ed 2007 46 7548
[9] S T Selvan P K Patra C Y Ang J Y Ying Angew Chem Int Ed 2007 46 2448
[10] I S Lee N Lee J Park B H Kim Y W Yi T Kim T K Kim I H Lee S R Paik T
Hyeon J Am Chem Soc 2006 128 10658
[11] Y S Lin S H Wu Y Hung Y H Chou C Chang M L Lin C P Tsai C Y Mou Chem
Mater 2006 18 5170
[12] B H Wu H Zhang C Chen S C Lin N F Zheng Nano Res 2009 2 975
[13] J Kim J E Lee J Lee J H Yu B C Kim K An Y Hwang C Shin J G Park J Kim T
Hyeon J Am Chem Soc 2006 128 688
[14] S Santra H S Yang P H Holloway J T Stanley R A Mericle J Am Chem Soc 2005 127
1656
[15] D K Yi S T Selvan S S Lee G C Papaefthymiou D Kundaliya J Y Ying J Am Chem
Soc 2005 127 4990 65
[16] J H Gao B Zhang Y Gao Y Pan X X Zhang B Xu J Am Chem Soc 2007 129 11928 [17] J H Gao W Zhang P B Huang B Zhang X X Zhang B Xu J Am Chem Soc 2008 130 3710 [18] J H Gao G L Liang B Zhang Y Kuang X X Zhang B Xu J Am Chem Soc 2007 129 1428 [19] J H Gao G L Liang J S Cheung Y Pan Y Kuang F Zhao B Zhang X X Zhang E X Wu B Xu J Am Chem Soc 2008 130 11828 [20] A R Kortan R Hull R L Opila M G Bawendi M L Steigerwald P J Carroll L E Brus JAm Chem Soc 1990 112 1327 [21] X G Peng M C Schlamp A V Kadavanich A P Alivisatos J Am Chem Soc 1997 119 7019 [22] S Kim B Fisher H J Eisler M Bawendi J Am Chem Soc 2003 125 11466[23] H Zeng J Li Z L Wang J P Liu S Sun Nano Lett 2004 4 187 [24] H Zeng S Sun J Li Z L Wang J P Liu Appl Phys Lett 2004 85 792 [25] Z Xu Y Hou S Sun J Am Chem Soc 2007 129 8698 [26] Y H Lin N Kim J Q Wang C J Zhong J Phys Chem B 2005 109 21593 [27] C Wang H Daimon S Sun Nano Lett 2009 9 1493 [28] K W Kwon M Shim J Am Chem Soc 2005 127 10269 [29] H Yu M Chen P M Rice S X Wang R L White S Sun Nano Lett 2005 5 379 [30] J S Choi Y W Jun S I Yeon H C Kim J S Shin J Cheon J Am Chem Soc 2006 128 15982 [31] S H Choi H B Na Y I Park K An S G Kwon Y Jang M Park J Moon J S Son I CSong W K Moon T Hyeon J Am Chem Soc 2008 130 15573 [32] H W Gu R K Zheng X X Zhang B Xu J Am Chem Soc 2004 126 5664
66
15
Colloidal stability by citrate
Au Au
- ---- - -
- ---O O
OO O
O
O H
Lowis bond
16
Color is size dependent
Size(nm) Emission(nm) Suspension in
5 515-520 PBSsurfactant
10 515-520 PBSsurfactant
15 520 PBSsurfactant
20 524 PBSsurfactant
30 526 PBSsurfactant
40 530 PBSsurfactant
50 535 PBSsurfactant
60 540 PBSsurfactant
80 553 PBSsurfactant
100 572 PBSsurfactant
17
18
GNP Advantage
- Color size dependent
- No toxicity
- DNA strong bonding
- Thiol covalent bond
19
The surface Hydrophobicity can be modified by
thiol compound
20
The surface self-assembling by thiol compound
21
GNP Application
Different bio sensor based self assemble design
22
23
The Sol-Gel Process is
a method for producing solid materials from small molecules
inn RT The method is used for the fabrication of metal oxides
especially the oxides of silicon and titanium
24
Alkoxide-sol-gel-method-preparation
25
HydrolysisCondensation
26
27
Why silica sol-gel coating
- Biocompatible
- Functionalization
- Physical trapping
- Chemical stability
- Physical stability
28
I Byun AW Coleman B Kim Micromech Microeng 23 (2013) 085016 29
Three Dimensional
Multi Dimensional matrix
One Dimensional
Layer by layer coatingSi
OR
ORRORO
Si
OR
RORO
NH2
Si
OR
RORO
SH
Si
OR
RORO O
O
n
Si
OR
RORO
HN
NH
NH2
Si
R
RRORO
30
Two Dimensional
Physical trapping in sol gel
31
32
apto ldquoto fitrdquo
mer ldquosmallest unit of repeating structurerdquo
What is an Aptamer
Oligonucleotide or Peptide
molecules that bind to a specific
target molecule
33
Types of Aptamer
- ssDNA
- dsDNA
- RNA
- Peptide
34
SELEX(Systematic Evolution of Ligands by Exponential Enrichment)
Selection of ligand sequences that bind to a target
partitioning of aptamers from
non-aptamers via affinity methods
amplification of bound aptamers
35
Targets of Aptamers
- Small Molecules
- Proteins
- Nucleic Acids
- Cells
- Tissues
- Organisms
lysozym Thrombin HIV TAR Hemin Interferon γ Vascular
endotherial growth factor (VEGF) Prostate specific antigen(PSA)
Dopamine hellip 36
37
MFMNP Design
Core Shell
Dumbbell Structure
Multicomponent Hybrid NP
38
39
Polymer Shell
Core Shell
Metal alloy
(FePt)
Magnetic nanolayer
(Fe3O4)
Thermal decomposition
of Fe(acac)3 on FePt
Magnetic NP
(Fe3O4)
Metal alloy
(Au Ag)
(Fe3O4) in Au3+ solution
at reduction condition
40
1 Core Shell structure
41
- Mix of NP during the growth of nanocrystals
- Sol-gel process
Core Shell synthesis
2 Dumbbell structures
42
- Mix of NP during the growth of nanocrystals
- Thermal rearrangement
- self-assemble at a liquid-liquid interface
- Functional sol-gel matrix
3 Multicomponent Hybrid NPs
Self-assembly processes provide an approach to
fabricating multicomponent hybrid NPs with
integrated multifunctionality
43
- Surfactant polymers
- Sol-gel process
44
Poly(lactico-co-glycolicacid)
(PLGA)
Poly(L-lysine)-PEG-folate
(PLL-PEG-FOL)
By mechanical
stirring at RT
in CH3Cl
45
46
W Lu SR Arumugam D Senapati ACSNANO 2010 4(3) 1739ndash1749
GNP GNP
GNP
RNA Aptamer
Anti-HER2C-reb-2 antibody
GNPCTAB
47
Magnetic nanoparticle surface modification Glucose oxidasse
(GOx)
PEGGPS
APSMNP
48Z Lin et al Analytica Chimica Acta 818 (2014) 54ndash60
49
P Sun H Zhang C Liu Langmuir 2010 26(2) 1278ndash1284 50
Immuno-labeling using Fe3O4CdTe nanocomposites which are formed by
linking multiple TGA-stabilized CdTe QDs with the thiol-functionalized silica
coated iron oxide nanoparticles
23-dimercaptosuccinic
acids (DMSA)Dopamine
Mercaptoundecanoic acid
(MUA)
HS COOH
4 Small multifunctional molecules
Glutaraldehyde 51
C J Xu K M Xu H W Gu R K Zheng H Liu X X Zhang Z H Guo B Xu J Am Chem Soc 2004 126 9938
Dopamine
Conditions (a) NaOH tert-butyl dicarbonate dioxane H2O 24 hrs (b) BnBr K2CO3 DMF
RT 24 h (c) 10 CF3COOH CH2Cl2 rt 5 h (d) succinic anhydride pyridine RTt 3 h (e)
NHS DCC DMAP CHCl3 RTt 3 h
NTA
52
3-mercapto trimethoxysilane
(MPS)
3-amino trimethoxysilane
(APS)
5 Silane multifunctional monomers
Glycidoxypropyltrimethoxy-silane
(GPS)
53
54
Multifunctional magnetic nanoparticles
application
Bioseparation
Biodetection(Real time monitoring)
Targeted drug delivery
Magnetic fluid Hyperthermia therapy
55
1 Specific Bio-Separation
bull Magnetic properties
bull Targeting agent
Cell organism molecule
56
His-tagged protein purification as
the first step towards recombinant
protein purification
58
Lysing Binding
Nickel
affinity
nanoparicle
His tag
fusion protein
Washing Elusion
Digestion
Binding
Nickel
affinity
nanoparticle
Washing Target
Recombinant
Protein
1- Preparation of Fe3O4SiO2Layered Double Hydroxide CoreminusShell
Microspheres for Magnetic Separation of Proteins J Am ChemSoc (2012)
18 134(2) 1071-7
Ni2+
2- Magnetic Nanocomposite Spheres Decorated with NiO Nanoparticles for a
Magnetically Recyclable Protein Separation System J Kim Y Piao N Lee Y
Park I-H Lee J-H Lee Adv Mater (2010) 22 57ndash60
His-tagged protein purification
Purification of His6-lipase using Fe3O4AundashLAndashIDAndashCu2+ NPs with various
amounts of NPs (lane 1 01 mg lane 2 02 mg lane 3
63
Synthetic procedure for the coreshell structure spheres
64
glutaraldehyde
(GLH)
Reference[1] H W Gu Z M Yang J H Gao C K Chang B Xu J Am Chem Soc 2005 127 34
[2] A Hutten D Sudfeld I Ennen G Reiss K Wojczykowski P Jutzi J Magn Magn Mater 2005
293 93
[3] G S Chaubey C Barcena N Poudyal C B Rong J M Gao S Sun J P Liu J Am Chem
Soc 2007 129 7214
[4] C W Kim Y H Kim H G Cha D K Lee Y S Kang J NanosciNanotechnol 2006 6 3417
[5] Y W Jun J S Choi J Cheon Angew Chem Int Ed 2006 45 3414
[6] X Michalet F F Pinaud L A Bentolila J M Tsay S Doose J J Li G Sundaresan A M Wu
S S Gambhir S Weiss Science 2005 307 538
[7] M Hu J Y Chen Z Y Li L Au G V Hartland X D Li M Marquez Y N Xia Chem Soc
Rev 2006 35 1084
[8] M Vallet-Regi F Balas D Arcos Angew Chem Int Ed 2007 46 7548
[9] S T Selvan P K Patra C Y Ang J Y Ying Angew Chem Int Ed 2007 46 2448
[10] I S Lee N Lee J Park B H Kim Y W Yi T Kim T K Kim I H Lee S R Paik T
Hyeon J Am Chem Soc 2006 128 10658
[11] Y S Lin S H Wu Y Hung Y H Chou C Chang M L Lin C P Tsai C Y Mou Chem
Mater 2006 18 5170
[12] B H Wu H Zhang C Chen S C Lin N F Zheng Nano Res 2009 2 975
[13] J Kim J E Lee J Lee J H Yu B C Kim K An Y Hwang C Shin J G Park J Kim T
Hyeon J Am Chem Soc 2006 128 688
[14] S Santra H S Yang P H Holloway J T Stanley R A Mericle J Am Chem Soc 2005 127
1656
[15] D K Yi S T Selvan S S Lee G C Papaefthymiou D Kundaliya J Y Ying J Am Chem
Soc 2005 127 4990 65
[16] J H Gao B Zhang Y Gao Y Pan X X Zhang B Xu J Am Chem Soc 2007 129 11928 [17] J H Gao W Zhang P B Huang B Zhang X X Zhang B Xu J Am Chem Soc 2008 130 3710 [18] J H Gao G L Liang B Zhang Y Kuang X X Zhang B Xu J Am Chem Soc 2007 129 1428 [19] J H Gao G L Liang J S Cheung Y Pan Y Kuang F Zhao B Zhang X X Zhang E X Wu B Xu J Am Chem Soc 2008 130 11828 [20] A R Kortan R Hull R L Opila M G Bawendi M L Steigerwald P J Carroll L E Brus JAm Chem Soc 1990 112 1327 [21] X G Peng M C Schlamp A V Kadavanich A P Alivisatos J Am Chem Soc 1997 119 7019 [22] S Kim B Fisher H J Eisler M Bawendi J Am Chem Soc 2003 125 11466[23] H Zeng J Li Z L Wang J P Liu S Sun Nano Lett 2004 4 187 [24] H Zeng S Sun J Li Z L Wang J P Liu Appl Phys Lett 2004 85 792 [25] Z Xu Y Hou S Sun J Am Chem Soc 2007 129 8698 [26] Y H Lin N Kim J Q Wang C J Zhong J Phys Chem B 2005 109 21593 [27] C Wang H Daimon S Sun Nano Lett 2009 9 1493 [28] K W Kwon M Shim J Am Chem Soc 2005 127 10269 [29] H Yu M Chen P M Rice S X Wang R L White S Sun Nano Lett 2005 5 379 [30] J S Choi Y W Jun S I Yeon H C Kim J S Shin J Cheon J Am Chem Soc 2006 128 15982 [31] S H Choi H B Na Y I Park K An S G Kwon Y Jang M Park J Moon J S Son I CSong W K Moon T Hyeon J Am Chem Soc 2008 130 15573 [32] H W Gu R K Zheng X X Zhang B Xu J Am Chem Soc 2004 126 5664
66
Colloidal stability by citrate
Au Au
- ---- - -
- ---O O
OO O
O
O H
Lowis bond
16
Color is size dependent
Size(nm) Emission(nm) Suspension in
5 515-520 PBSsurfactant
10 515-520 PBSsurfactant
15 520 PBSsurfactant
20 524 PBSsurfactant
30 526 PBSsurfactant
40 530 PBSsurfactant
50 535 PBSsurfactant
60 540 PBSsurfactant
80 553 PBSsurfactant
100 572 PBSsurfactant
17
18
GNP Advantage
- Color size dependent
- No toxicity
- DNA strong bonding
- Thiol covalent bond
19
The surface Hydrophobicity can be modified by
thiol compound
20
The surface self-assembling by thiol compound
21
GNP Application
Different bio sensor based self assemble design
22
23
The Sol-Gel Process is
a method for producing solid materials from small molecules
inn RT The method is used for the fabrication of metal oxides
especially the oxides of silicon and titanium
24
Alkoxide-sol-gel-method-preparation
25
HydrolysisCondensation
26
27
Why silica sol-gel coating
- Biocompatible
- Functionalization
- Physical trapping
- Chemical stability
- Physical stability
28
I Byun AW Coleman B Kim Micromech Microeng 23 (2013) 085016 29
Three Dimensional
Multi Dimensional matrix
One Dimensional
Layer by layer coatingSi
OR
ORRORO
Si
OR
RORO
NH2
Si
OR
RORO
SH
Si
OR
RORO O
O
n
Si
OR
RORO
HN
NH
NH2
Si
R
RRORO
30
Two Dimensional
Physical trapping in sol gel
31
32
apto ldquoto fitrdquo
mer ldquosmallest unit of repeating structurerdquo
What is an Aptamer
Oligonucleotide or Peptide
molecules that bind to a specific
target molecule
33
Types of Aptamer
- ssDNA
- dsDNA
- RNA
- Peptide
34
SELEX(Systematic Evolution of Ligands by Exponential Enrichment)
Selection of ligand sequences that bind to a target
partitioning of aptamers from
non-aptamers via affinity methods
amplification of bound aptamers
35
Targets of Aptamers
- Small Molecules
- Proteins
- Nucleic Acids
- Cells
- Tissues
- Organisms
lysozym Thrombin HIV TAR Hemin Interferon γ Vascular
endotherial growth factor (VEGF) Prostate specific antigen(PSA)
Dopamine hellip 36
37
MFMNP Design
Core Shell
Dumbbell Structure
Multicomponent Hybrid NP
38
39
Polymer Shell
Core Shell
Metal alloy
(FePt)
Magnetic nanolayer
(Fe3O4)
Thermal decomposition
of Fe(acac)3 on FePt
Magnetic NP
(Fe3O4)
Metal alloy
(Au Ag)
(Fe3O4) in Au3+ solution
at reduction condition
40
1 Core Shell structure
41
- Mix of NP during the growth of nanocrystals
- Sol-gel process
Core Shell synthesis
2 Dumbbell structures
42
- Mix of NP during the growth of nanocrystals
- Thermal rearrangement
- self-assemble at a liquid-liquid interface
- Functional sol-gel matrix
3 Multicomponent Hybrid NPs
Self-assembly processes provide an approach to
fabricating multicomponent hybrid NPs with
integrated multifunctionality
43
- Surfactant polymers
- Sol-gel process
44
Poly(lactico-co-glycolicacid)
(PLGA)
Poly(L-lysine)-PEG-folate
(PLL-PEG-FOL)
By mechanical
stirring at RT
in CH3Cl
45
46
W Lu SR Arumugam D Senapati ACSNANO 2010 4(3) 1739ndash1749
GNP GNP
GNP
RNA Aptamer
Anti-HER2C-reb-2 antibody
GNPCTAB
47
Magnetic nanoparticle surface modification Glucose oxidasse
(GOx)
PEGGPS
APSMNP
48Z Lin et al Analytica Chimica Acta 818 (2014) 54ndash60
49
P Sun H Zhang C Liu Langmuir 2010 26(2) 1278ndash1284 50
Immuno-labeling using Fe3O4CdTe nanocomposites which are formed by
linking multiple TGA-stabilized CdTe QDs with the thiol-functionalized silica
coated iron oxide nanoparticles
23-dimercaptosuccinic
acids (DMSA)Dopamine
Mercaptoundecanoic acid
(MUA)
HS COOH
4 Small multifunctional molecules
Glutaraldehyde 51
C J Xu K M Xu H W Gu R K Zheng H Liu X X Zhang Z H Guo B Xu J Am Chem Soc 2004 126 9938
Dopamine
Conditions (a) NaOH tert-butyl dicarbonate dioxane H2O 24 hrs (b) BnBr K2CO3 DMF
RT 24 h (c) 10 CF3COOH CH2Cl2 rt 5 h (d) succinic anhydride pyridine RTt 3 h (e)
NHS DCC DMAP CHCl3 RTt 3 h
NTA
52
3-mercapto trimethoxysilane
(MPS)
3-amino trimethoxysilane
(APS)
5 Silane multifunctional monomers
Glycidoxypropyltrimethoxy-silane
(GPS)
53
54
Multifunctional magnetic nanoparticles
application
Bioseparation
Biodetection(Real time monitoring)
Targeted drug delivery
Magnetic fluid Hyperthermia therapy
55
1 Specific Bio-Separation
bull Magnetic properties
bull Targeting agent
Cell organism molecule
56
His-tagged protein purification as
the first step towards recombinant
protein purification
58
Lysing Binding
Nickel
affinity
nanoparicle
His tag
fusion protein
Washing Elusion
Digestion
Binding
Nickel
affinity
nanoparticle
Washing Target
Recombinant
Protein
1- Preparation of Fe3O4SiO2Layered Double Hydroxide CoreminusShell
Microspheres for Magnetic Separation of Proteins J Am ChemSoc (2012)
18 134(2) 1071-7
Ni2+
2- Magnetic Nanocomposite Spheres Decorated with NiO Nanoparticles for a
Magnetically Recyclable Protein Separation System J Kim Y Piao N Lee Y
Park I-H Lee J-H Lee Adv Mater (2010) 22 57ndash60
His-tagged protein purification
Purification of His6-lipase using Fe3O4AundashLAndashIDAndashCu2+ NPs with various
amounts of NPs (lane 1 01 mg lane 2 02 mg lane 3
63
Synthetic procedure for the coreshell structure spheres
64
glutaraldehyde
(GLH)
Reference[1] H W Gu Z M Yang J H Gao C K Chang B Xu J Am Chem Soc 2005 127 34
[2] A Hutten D Sudfeld I Ennen G Reiss K Wojczykowski P Jutzi J Magn Magn Mater 2005
293 93
[3] G S Chaubey C Barcena N Poudyal C B Rong J M Gao S Sun J P Liu J Am Chem
Soc 2007 129 7214
[4] C W Kim Y H Kim H G Cha D K Lee Y S Kang J NanosciNanotechnol 2006 6 3417
[5] Y W Jun J S Choi J Cheon Angew Chem Int Ed 2006 45 3414
[6] X Michalet F F Pinaud L A Bentolila J M Tsay S Doose J J Li G Sundaresan A M Wu
S S Gambhir S Weiss Science 2005 307 538
[7] M Hu J Y Chen Z Y Li L Au G V Hartland X D Li M Marquez Y N Xia Chem Soc
Rev 2006 35 1084
[8] M Vallet-Regi F Balas D Arcos Angew Chem Int Ed 2007 46 7548
[9] S T Selvan P K Patra C Y Ang J Y Ying Angew Chem Int Ed 2007 46 2448
[10] I S Lee N Lee J Park B H Kim Y W Yi T Kim T K Kim I H Lee S R Paik T
Hyeon J Am Chem Soc 2006 128 10658
[11] Y S Lin S H Wu Y Hung Y H Chou C Chang M L Lin C P Tsai C Y Mou Chem
Mater 2006 18 5170
[12] B H Wu H Zhang C Chen S C Lin N F Zheng Nano Res 2009 2 975
[13] J Kim J E Lee J Lee J H Yu B C Kim K An Y Hwang C Shin J G Park J Kim T
Hyeon J Am Chem Soc 2006 128 688
[14] S Santra H S Yang P H Holloway J T Stanley R A Mericle J Am Chem Soc 2005 127
1656
[15] D K Yi S T Selvan S S Lee G C Papaefthymiou D Kundaliya J Y Ying J Am Chem
Soc 2005 127 4990 65
[16] J H Gao B Zhang Y Gao Y Pan X X Zhang B Xu J Am Chem Soc 2007 129 11928 [17] J H Gao W Zhang P B Huang B Zhang X X Zhang B Xu J Am Chem Soc 2008 130 3710 [18] J H Gao G L Liang B Zhang Y Kuang X X Zhang B Xu J Am Chem Soc 2007 129 1428 [19] J H Gao G L Liang J S Cheung Y Pan Y Kuang F Zhao B Zhang X X Zhang E X Wu B Xu J Am Chem Soc 2008 130 11828 [20] A R Kortan R Hull R L Opila M G Bawendi M L Steigerwald P J Carroll L E Brus JAm Chem Soc 1990 112 1327 [21] X G Peng M C Schlamp A V Kadavanich A P Alivisatos J Am Chem Soc 1997 119 7019 [22] S Kim B Fisher H J Eisler M Bawendi J Am Chem Soc 2003 125 11466[23] H Zeng J Li Z L Wang J P Liu S Sun Nano Lett 2004 4 187 [24] H Zeng S Sun J Li Z L Wang J P Liu Appl Phys Lett 2004 85 792 [25] Z Xu Y Hou S Sun J Am Chem Soc 2007 129 8698 [26] Y H Lin N Kim J Q Wang C J Zhong J Phys Chem B 2005 109 21593 [27] C Wang H Daimon S Sun Nano Lett 2009 9 1493 [28] K W Kwon M Shim J Am Chem Soc 2005 127 10269 [29] H Yu M Chen P M Rice S X Wang R L White S Sun Nano Lett 2005 5 379 [30] J S Choi Y W Jun S I Yeon H C Kim J S Shin J Cheon J Am Chem Soc 2006 128 15982 [31] S H Choi H B Na Y I Park K An S G Kwon Y Jang M Park J Moon J S Son I CSong W K Moon T Hyeon J Am Chem Soc 2008 130 15573 [32] H W Gu R K Zheng X X Zhang B Xu J Am Chem Soc 2004 126 5664
66
Color is size dependent
Size(nm) Emission(nm) Suspension in
5 515-520 PBSsurfactant
10 515-520 PBSsurfactant
15 520 PBSsurfactant
20 524 PBSsurfactant
30 526 PBSsurfactant
40 530 PBSsurfactant
50 535 PBSsurfactant
60 540 PBSsurfactant
80 553 PBSsurfactant
100 572 PBSsurfactant
17
18
GNP Advantage
- Color size dependent
- No toxicity
- DNA strong bonding
- Thiol covalent bond
19
The surface Hydrophobicity can be modified by
thiol compound
20
The surface self-assembling by thiol compound
21
GNP Application
Different bio sensor based self assemble design
22
23
The Sol-Gel Process is
a method for producing solid materials from small molecules
inn RT The method is used for the fabrication of metal oxides
especially the oxides of silicon and titanium
24
Alkoxide-sol-gel-method-preparation
25
HydrolysisCondensation
26
27
Why silica sol-gel coating
- Biocompatible
- Functionalization
- Physical trapping
- Chemical stability
- Physical stability
28
I Byun AW Coleman B Kim Micromech Microeng 23 (2013) 085016 29
Three Dimensional
Multi Dimensional matrix
One Dimensional
Layer by layer coatingSi
OR
ORRORO
Si
OR
RORO
NH2
Si
OR
RORO
SH
Si
OR
RORO O
O
n
Si
OR
RORO
HN
NH
NH2
Si
R
RRORO
30
Two Dimensional
Physical trapping in sol gel
31
32
apto ldquoto fitrdquo
mer ldquosmallest unit of repeating structurerdquo
What is an Aptamer
Oligonucleotide or Peptide
molecules that bind to a specific
target molecule
33
Types of Aptamer
- ssDNA
- dsDNA
- RNA
- Peptide
34
SELEX(Systematic Evolution of Ligands by Exponential Enrichment)
Selection of ligand sequences that bind to a target
partitioning of aptamers from
non-aptamers via affinity methods
amplification of bound aptamers
35
Targets of Aptamers
- Small Molecules
- Proteins
- Nucleic Acids
- Cells
- Tissues
- Organisms
lysozym Thrombin HIV TAR Hemin Interferon γ Vascular
endotherial growth factor (VEGF) Prostate specific antigen(PSA)
Dopamine hellip 36
37
MFMNP Design
Core Shell
Dumbbell Structure
Multicomponent Hybrid NP
38
39
Polymer Shell
Core Shell
Metal alloy
(FePt)
Magnetic nanolayer
(Fe3O4)
Thermal decomposition
of Fe(acac)3 on FePt
Magnetic NP
(Fe3O4)
Metal alloy
(Au Ag)
(Fe3O4) in Au3+ solution
at reduction condition
40
1 Core Shell structure
41
- Mix of NP during the growth of nanocrystals
- Sol-gel process
Core Shell synthesis
2 Dumbbell structures
42
- Mix of NP during the growth of nanocrystals
- Thermal rearrangement
- self-assemble at a liquid-liquid interface
- Functional sol-gel matrix
3 Multicomponent Hybrid NPs
Self-assembly processes provide an approach to
fabricating multicomponent hybrid NPs with
integrated multifunctionality
43
- Surfactant polymers
- Sol-gel process
44
Poly(lactico-co-glycolicacid)
(PLGA)
Poly(L-lysine)-PEG-folate
(PLL-PEG-FOL)
By mechanical
stirring at RT
in CH3Cl
45
46
W Lu SR Arumugam D Senapati ACSNANO 2010 4(3) 1739ndash1749
GNP GNP
GNP
RNA Aptamer
Anti-HER2C-reb-2 antibody
GNPCTAB
47
Magnetic nanoparticle surface modification Glucose oxidasse
(GOx)
PEGGPS
APSMNP
48Z Lin et al Analytica Chimica Acta 818 (2014) 54ndash60
49
P Sun H Zhang C Liu Langmuir 2010 26(2) 1278ndash1284 50
Immuno-labeling using Fe3O4CdTe nanocomposites which are formed by
linking multiple TGA-stabilized CdTe QDs with the thiol-functionalized silica
coated iron oxide nanoparticles
23-dimercaptosuccinic
acids (DMSA)Dopamine
Mercaptoundecanoic acid
(MUA)
HS COOH
4 Small multifunctional molecules
Glutaraldehyde 51
C J Xu K M Xu H W Gu R K Zheng H Liu X X Zhang Z H Guo B Xu J Am Chem Soc 2004 126 9938
Dopamine
Conditions (a) NaOH tert-butyl dicarbonate dioxane H2O 24 hrs (b) BnBr K2CO3 DMF
RT 24 h (c) 10 CF3COOH CH2Cl2 rt 5 h (d) succinic anhydride pyridine RTt 3 h (e)
NHS DCC DMAP CHCl3 RTt 3 h
NTA
52
3-mercapto trimethoxysilane
(MPS)
3-amino trimethoxysilane
(APS)
5 Silane multifunctional monomers
Glycidoxypropyltrimethoxy-silane
(GPS)
53
54
Multifunctional magnetic nanoparticles
application
Bioseparation
Biodetection(Real time monitoring)
Targeted drug delivery
Magnetic fluid Hyperthermia therapy
55
1 Specific Bio-Separation
bull Magnetic properties
bull Targeting agent
Cell organism molecule
56
His-tagged protein purification as
the first step towards recombinant
protein purification
58
Lysing Binding
Nickel
affinity
nanoparicle
His tag
fusion protein
Washing Elusion
Digestion
Binding
Nickel
affinity
nanoparticle
Washing Target
Recombinant
Protein
1- Preparation of Fe3O4SiO2Layered Double Hydroxide CoreminusShell
Microspheres for Magnetic Separation of Proteins J Am ChemSoc (2012)
18 134(2) 1071-7
Ni2+
2- Magnetic Nanocomposite Spheres Decorated with NiO Nanoparticles for a
Magnetically Recyclable Protein Separation System J Kim Y Piao N Lee Y
Park I-H Lee J-H Lee Adv Mater (2010) 22 57ndash60
His-tagged protein purification
Purification of His6-lipase using Fe3O4AundashLAndashIDAndashCu2+ NPs with various
amounts of NPs (lane 1 01 mg lane 2 02 mg lane 3
63
Synthetic procedure for the coreshell structure spheres
64
glutaraldehyde
(GLH)
Reference[1] H W Gu Z M Yang J H Gao C K Chang B Xu J Am Chem Soc 2005 127 34
[2] A Hutten D Sudfeld I Ennen G Reiss K Wojczykowski P Jutzi J Magn Magn Mater 2005
293 93
[3] G S Chaubey C Barcena N Poudyal C B Rong J M Gao S Sun J P Liu J Am Chem
Soc 2007 129 7214
[4] C W Kim Y H Kim H G Cha D K Lee Y S Kang J NanosciNanotechnol 2006 6 3417
[5] Y W Jun J S Choi J Cheon Angew Chem Int Ed 2006 45 3414
[6] X Michalet F F Pinaud L A Bentolila J M Tsay S Doose J J Li G Sundaresan A M Wu
S S Gambhir S Weiss Science 2005 307 538
[7] M Hu J Y Chen Z Y Li L Au G V Hartland X D Li M Marquez Y N Xia Chem Soc
Rev 2006 35 1084
[8] M Vallet-Regi F Balas D Arcos Angew Chem Int Ed 2007 46 7548
[9] S T Selvan P K Patra C Y Ang J Y Ying Angew Chem Int Ed 2007 46 2448
[10] I S Lee N Lee J Park B H Kim Y W Yi T Kim T K Kim I H Lee S R Paik T
Hyeon J Am Chem Soc 2006 128 10658
[11] Y S Lin S H Wu Y Hung Y H Chou C Chang M L Lin C P Tsai C Y Mou Chem
Mater 2006 18 5170
[12] B H Wu H Zhang C Chen S C Lin N F Zheng Nano Res 2009 2 975
[13] J Kim J E Lee J Lee J H Yu B C Kim K An Y Hwang C Shin J G Park J Kim T
Hyeon J Am Chem Soc 2006 128 688
[14] S Santra H S Yang P H Holloway J T Stanley R A Mericle J Am Chem Soc 2005 127
1656
[15] D K Yi S T Selvan S S Lee G C Papaefthymiou D Kundaliya J Y Ying J Am Chem
Soc 2005 127 4990 65
[16] J H Gao B Zhang Y Gao Y Pan X X Zhang B Xu J Am Chem Soc 2007 129 11928 [17] J H Gao W Zhang P B Huang B Zhang X X Zhang B Xu J Am Chem Soc 2008 130 3710 [18] J H Gao G L Liang B Zhang Y Kuang X X Zhang B Xu J Am Chem Soc 2007 129 1428 [19] J H Gao G L Liang J S Cheung Y Pan Y Kuang F Zhao B Zhang X X Zhang E X Wu B Xu J Am Chem Soc 2008 130 11828 [20] A R Kortan R Hull R L Opila M G Bawendi M L Steigerwald P J Carroll L E Brus JAm Chem Soc 1990 112 1327 [21] X G Peng M C Schlamp A V Kadavanich A P Alivisatos J Am Chem Soc 1997 119 7019 [22] S Kim B Fisher H J Eisler M Bawendi J Am Chem Soc 2003 125 11466[23] H Zeng J Li Z L Wang J P Liu S Sun Nano Lett 2004 4 187 [24] H Zeng S Sun J Li Z L Wang J P Liu Appl Phys Lett 2004 85 792 [25] Z Xu Y Hou S Sun J Am Chem Soc 2007 129 8698 [26] Y H Lin N Kim J Q Wang C J Zhong J Phys Chem B 2005 109 21593 [27] C Wang H Daimon S Sun Nano Lett 2009 9 1493 [28] K W Kwon M Shim J Am Chem Soc 2005 127 10269 [29] H Yu M Chen P M Rice S X Wang R L White S Sun Nano Lett 2005 5 379 [30] J S Choi Y W Jun S I Yeon H C Kim J S Shin J Cheon J Am Chem Soc 2006 128 15982 [31] S H Choi H B Na Y I Park K An S G Kwon Y Jang M Park J Moon J S Son I CSong W K Moon T Hyeon J Am Chem Soc 2008 130 15573 [32] H W Gu R K Zheng X X Zhang B Xu J Am Chem Soc 2004 126 5664
66
18
GNP Advantage
- Color size dependent
- No toxicity
- DNA strong bonding
- Thiol covalent bond
19
The surface Hydrophobicity can be modified by
thiol compound
20
The surface self-assembling by thiol compound
21
GNP Application
Different bio sensor based self assemble design
22
23
The Sol-Gel Process is
a method for producing solid materials from small molecules
inn RT The method is used for the fabrication of metal oxides
especially the oxides of silicon and titanium
24
Alkoxide-sol-gel-method-preparation
25
HydrolysisCondensation
26
27
Why silica sol-gel coating
- Biocompatible
- Functionalization
- Physical trapping
- Chemical stability
- Physical stability
28
I Byun AW Coleman B Kim Micromech Microeng 23 (2013) 085016 29
Three Dimensional
Multi Dimensional matrix
One Dimensional
Layer by layer coatingSi
OR
ORRORO
Si
OR
RORO
NH2
Si
OR
RORO
SH
Si
OR
RORO O
O
n
Si
OR
RORO
HN
NH
NH2
Si
R
RRORO
30
Two Dimensional
Physical trapping in sol gel
31
32
apto ldquoto fitrdquo
mer ldquosmallest unit of repeating structurerdquo
What is an Aptamer
Oligonucleotide or Peptide
molecules that bind to a specific
target molecule
33
Types of Aptamer
- ssDNA
- dsDNA
- RNA
- Peptide
34
SELEX(Systematic Evolution of Ligands by Exponential Enrichment)
Selection of ligand sequences that bind to a target
partitioning of aptamers from
non-aptamers via affinity methods
amplification of bound aptamers
35
Targets of Aptamers
- Small Molecules
- Proteins
- Nucleic Acids
- Cells
- Tissues
- Organisms
lysozym Thrombin HIV TAR Hemin Interferon γ Vascular
endotherial growth factor (VEGF) Prostate specific antigen(PSA)
Dopamine hellip 36
37
MFMNP Design
Core Shell
Dumbbell Structure
Multicomponent Hybrid NP
38
39
Polymer Shell
Core Shell
Metal alloy
(FePt)
Magnetic nanolayer
(Fe3O4)
Thermal decomposition
of Fe(acac)3 on FePt
Magnetic NP
(Fe3O4)
Metal alloy
(Au Ag)
(Fe3O4) in Au3+ solution
at reduction condition
40
1 Core Shell structure
41
- Mix of NP during the growth of nanocrystals
- Sol-gel process
Core Shell synthesis
2 Dumbbell structures
42
- Mix of NP during the growth of nanocrystals
- Thermal rearrangement
- self-assemble at a liquid-liquid interface
- Functional sol-gel matrix
3 Multicomponent Hybrid NPs
Self-assembly processes provide an approach to
fabricating multicomponent hybrid NPs with
integrated multifunctionality
43
- Surfactant polymers
- Sol-gel process
44
Poly(lactico-co-glycolicacid)
(PLGA)
Poly(L-lysine)-PEG-folate
(PLL-PEG-FOL)
By mechanical
stirring at RT
in CH3Cl
45
46
W Lu SR Arumugam D Senapati ACSNANO 2010 4(3) 1739ndash1749
GNP GNP
GNP
RNA Aptamer
Anti-HER2C-reb-2 antibody
GNPCTAB
47
Magnetic nanoparticle surface modification Glucose oxidasse
(GOx)
PEGGPS
APSMNP
48Z Lin et al Analytica Chimica Acta 818 (2014) 54ndash60
49
P Sun H Zhang C Liu Langmuir 2010 26(2) 1278ndash1284 50
Immuno-labeling using Fe3O4CdTe nanocomposites which are formed by
linking multiple TGA-stabilized CdTe QDs with the thiol-functionalized silica
coated iron oxide nanoparticles
23-dimercaptosuccinic
acids (DMSA)Dopamine
Mercaptoundecanoic acid
(MUA)
HS COOH
4 Small multifunctional molecules
Glutaraldehyde 51
C J Xu K M Xu H W Gu R K Zheng H Liu X X Zhang Z H Guo B Xu J Am Chem Soc 2004 126 9938
Dopamine
Conditions (a) NaOH tert-butyl dicarbonate dioxane H2O 24 hrs (b) BnBr K2CO3 DMF
RT 24 h (c) 10 CF3COOH CH2Cl2 rt 5 h (d) succinic anhydride pyridine RTt 3 h (e)
NHS DCC DMAP CHCl3 RTt 3 h
NTA
52
3-mercapto trimethoxysilane
(MPS)
3-amino trimethoxysilane
(APS)
5 Silane multifunctional monomers
Glycidoxypropyltrimethoxy-silane
(GPS)
53
54
Multifunctional magnetic nanoparticles
application
Bioseparation
Biodetection(Real time monitoring)
Targeted drug delivery
Magnetic fluid Hyperthermia therapy
55
1 Specific Bio-Separation
bull Magnetic properties
bull Targeting agent
Cell organism molecule
56
His-tagged protein purification as
the first step towards recombinant
protein purification
58
Lysing Binding
Nickel
affinity
nanoparicle
His tag
fusion protein
Washing Elusion
Digestion
Binding
Nickel
affinity
nanoparticle
Washing Target
Recombinant
Protein
1- Preparation of Fe3O4SiO2Layered Double Hydroxide CoreminusShell
Microspheres for Magnetic Separation of Proteins J Am ChemSoc (2012)
18 134(2) 1071-7
Ni2+
2- Magnetic Nanocomposite Spheres Decorated with NiO Nanoparticles for a
Magnetically Recyclable Protein Separation System J Kim Y Piao N Lee Y
Park I-H Lee J-H Lee Adv Mater (2010) 22 57ndash60
His-tagged protein purification
Purification of His6-lipase using Fe3O4AundashLAndashIDAndashCu2+ NPs with various
amounts of NPs (lane 1 01 mg lane 2 02 mg lane 3
63
Synthetic procedure for the coreshell structure spheres
64
glutaraldehyde
(GLH)
Reference[1] H W Gu Z M Yang J H Gao C K Chang B Xu J Am Chem Soc 2005 127 34
[2] A Hutten D Sudfeld I Ennen G Reiss K Wojczykowski P Jutzi J Magn Magn Mater 2005
293 93
[3] G S Chaubey C Barcena N Poudyal C B Rong J M Gao S Sun J P Liu J Am Chem
Soc 2007 129 7214
[4] C W Kim Y H Kim H G Cha D K Lee Y S Kang J NanosciNanotechnol 2006 6 3417
[5] Y W Jun J S Choi J Cheon Angew Chem Int Ed 2006 45 3414
[6] X Michalet F F Pinaud L A Bentolila J M Tsay S Doose J J Li G Sundaresan A M Wu
S S Gambhir S Weiss Science 2005 307 538
[7] M Hu J Y Chen Z Y Li L Au G V Hartland X D Li M Marquez Y N Xia Chem Soc
Rev 2006 35 1084
[8] M Vallet-Regi F Balas D Arcos Angew Chem Int Ed 2007 46 7548
[9] S T Selvan P K Patra C Y Ang J Y Ying Angew Chem Int Ed 2007 46 2448
[10] I S Lee N Lee J Park B H Kim Y W Yi T Kim T K Kim I H Lee S R Paik T
Hyeon J Am Chem Soc 2006 128 10658
[11] Y S Lin S H Wu Y Hung Y H Chou C Chang M L Lin C P Tsai C Y Mou Chem
Mater 2006 18 5170
[12] B H Wu H Zhang C Chen S C Lin N F Zheng Nano Res 2009 2 975
[13] J Kim J E Lee J Lee J H Yu B C Kim K An Y Hwang C Shin J G Park J Kim T
Hyeon J Am Chem Soc 2006 128 688
[14] S Santra H S Yang P H Holloway J T Stanley R A Mericle J Am Chem Soc 2005 127
1656
[15] D K Yi S T Selvan S S Lee G C Papaefthymiou D Kundaliya J Y Ying J Am Chem
Soc 2005 127 4990 65
[16] J H Gao B Zhang Y Gao Y Pan X X Zhang B Xu J Am Chem Soc 2007 129 11928 [17] J H Gao W Zhang P B Huang B Zhang X X Zhang B Xu J Am Chem Soc 2008 130 3710 [18] J H Gao G L Liang B Zhang Y Kuang X X Zhang B Xu J Am Chem Soc 2007 129 1428 [19] J H Gao G L Liang J S Cheung Y Pan Y Kuang F Zhao B Zhang X X Zhang E X Wu B Xu J Am Chem Soc 2008 130 11828 [20] A R Kortan R Hull R L Opila M G Bawendi M L Steigerwald P J Carroll L E Brus JAm Chem Soc 1990 112 1327 [21] X G Peng M C Schlamp A V Kadavanich A P Alivisatos J Am Chem Soc 1997 119 7019 [22] S Kim B Fisher H J Eisler M Bawendi J Am Chem Soc 2003 125 11466[23] H Zeng J Li Z L Wang J P Liu S Sun Nano Lett 2004 4 187 [24] H Zeng S Sun J Li Z L Wang J P Liu Appl Phys Lett 2004 85 792 [25] Z Xu Y Hou S Sun J Am Chem Soc 2007 129 8698 [26] Y H Lin N Kim J Q Wang C J Zhong J Phys Chem B 2005 109 21593 [27] C Wang H Daimon S Sun Nano Lett 2009 9 1493 [28] K W Kwon M Shim J Am Chem Soc 2005 127 10269 [29] H Yu M Chen P M Rice S X Wang R L White S Sun Nano Lett 2005 5 379 [30] J S Choi Y W Jun S I Yeon H C Kim J S Shin J Cheon J Am Chem Soc 2006 128 15982 [31] S H Choi H B Na Y I Park K An S G Kwon Y Jang M Park J Moon J S Son I CSong W K Moon T Hyeon J Am Chem Soc 2008 130 15573 [32] H W Gu R K Zheng X X Zhang B Xu J Am Chem Soc 2004 126 5664
66
GNP Advantage
- Color size dependent
- No toxicity
- DNA strong bonding
- Thiol covalent bond
19
The surface Hydrophobicity can be modified by
thiol compound
20
The surface self-assembling by thiol compound
21
GNP Application
Different bio sensor based self assemble design
22
23
The Sol-Gel Process is
a method for producing solid materials from small molecules
inn RT The method is used for the fabrication of metal oxides
especially the oxides of silicon and titanium
24
Alkoxide-sol-gel-method-preparation
25
HydrolysisCondensation
26
27
Why silica sol-gel coating
- Biocompatible
- Functionalization
- Physical trapping
- Chemical stability
- Physical stability
28
I Byun AW Coleman B Kim Micromech Microeng 23 (2013) 085016 29
Three Dimensional
Multi Dimensional matrix
One Dimensional
Layer by layer coatingSi
OR
ORRORO
Si
OR
RORO
NH2
Si
OR
RORO
SH
Si
OR
RORO O
O
n
Si
OR
RORO
HN
NH
NH2
Si
R
RRORO
30
Two Dimensional
Physical trapping in sol gel
31
32
apto ldquoto fitrdquo
mer ldquosmallest unit of repeating structurerdquo
What is an Aptamer
Oligonucleotide or Peptide
molecules that bind to a specific
target molecule
33
Types of Aptamer
- ssDNA
- dsDNA
- RNA
- Peptide
34
SELEX(Systematic Evolution of Ligands by Exponential Enrichment)
Selection of ligand sequences that bind to a target
partitioning of aptamers from
non-aptamers via affinity methods
amplification of bound aptamers
35
Targets of Aptamers
- Small Molecules
- Proteins
- Nucleic Acids
- Cells
- Tissues
- Organisms
lysozym Thrombin HIV TAR Hemin Interferon γ Vascular
endotherial growth factor (VEGF) Prostate specific antigen(PSA)
Dopamine hellip 36
37
MFMNP Design
Core Shell
Dumbbell Structure
Multicomponent Hybrid NP
38
39
Polymer Shell
Core Shell
Metal alloy
(FePt)
Magnetic nanolayer
(Fe3O4)
Thermal decomposition
of Fe(acac)3 on FePt
Magnetic NP
(Fe3O4)
Metal alloy
(Au Ag)
(Fe3O4) in Au3+ solution
at reduction condition
40
1 Core Shell structure
41
- Mix of NP during the growth of nanocrystals
- Sol-gel process
Core Shell synthesis
2 Dumbbell structures
42
- Mix of NP during the growth of nanocrystals
- Thermal rearrangement
- self-assemble at a liquid-liquid interface
- Functional sol-gel matrix
3 Multicomponent Hybrid NPs
Self-assembly processes provide an approach to
fabricating multicomponent hybrid NPs with
integrated multifunctionality
43
- Surfactant polymers
- Sol-gel process
44
Poly(lactico-co-glycolicacid)
(PLGA)
Poly(L-lysine)-PEG-folate
(PLL-PEG-FOL)
By mechanical
stirring at RT
in CH3Cl
45
46
W Lu SR Arumugam D Senapati ACSNANO 2010 4(3) 1739ndash1749
GNP GNP
GNP
RNA Aptamer
Anti-HER2C-reb-2 antibody
GNPCTAB
47
Magnetic nanoparticle surface modification Glucose oxidasse
(GOx)
PEGGPS
APSMNP
48Z Lin et al Analytica Chimica Acta 818 (2014) 54ndash60
49
P Sun H Zhang C Liu Langmuir 2010 26(2) 1278ndash1284 50
Immuno-labeling using Fe3O4CdTe nanocomposites which are formed by
linking multiple TGA-stabilized CdTe QDs with the thiol-functionalized silica
coated iron oxide nanoparticles
23-dimercaptosuccinic
acids (DMSA)Dopamine
Mercaptoundecanoic acid
(MUA)
HS COOH
4 Small multifunctional molecules
Glutaraldehyde 51
C J Xu K M Xu H W Gu R K Zheng H Liu X X Zhang Z H Guo B Xu J Am Chem Soc 2004 126 9938
Dopamine
Conditions (a) NaOH tert-butyl dicarbonate dioxane H2O 24 hrs (b) BnBr K2CO3 DMF
RT 24 h (c) 10 CF3COOH CH2Cl2 rt 5 h (d) succinic anhydride pyridine RTt 3 h (e)
NHS DCC DMAP CHCl3 RTt 3 h
NTA
52
3-mercapto trimethoxysilane
(MPS)
3-amino trimethoxysilane
(APS)
5 Silane multifunctional monomers
Glycidoxypropyltrimethoxy-silane
(GPS)
53
54
Multifunctional magnetic nanoparticles
application
Bioseparation
Biodetection(Real time monitoring)
Targeted drug delivery
Magnetic fluid Hyperthermia therapy
55
1 Specific Bio-Separation
bull Magnetic properties
bull Targeting agent
Cell organism molecule
56
His-tagged protein purification as
the first step towards recombinant
protein purification
58
Lysing Binding
Nickel
affinity
nanoparicle
His tag
fusion protein
Washing Elusion
Digestion
Binding
Nickel
affinity
nanoparticle
Washing Target
Recombinant
Protein
1- Preparation of Fe3O4SiO2Layered Double Hydroxide CoreminusShell
Microspheres for Magnetic Separation of Proteins J Am ChemSoc (2012)
18 134(2) 1071-7
Ni2+
2- Magnetic Nanocomposite Spheres Decorated with NiO Nanoparticles for a
Magnetically Recyclable Protein Separation System J Kim Y Piao N Lee Y
Park I-H Lee J-H Lee Adv Mater (2010) 22 57ndash60
His-tagged protein purification
Purification of His6-lipase using Fe3O4AundashLAndashIDAndashCu2+ NPs with various
amounts of NPs (lane 1 01 mg lane 2 02 mg lane 3
63
Synthetic procedure for the coreshell structure spheres
64
glutaraldehyde
(GLH)
Reference[1] H W Gu Z M Yang J H Gao C K Chang B Xu J Am Chem Soc 2005 127 34
[2] A Hutten D Sudfeld I Ennen G Reiss K Wojczykowski P Jutzi J Magn Magn Mater 2005
293 93
[3] G S Chaubey C Barcena N Poudyal C B Rong J M Gao S Sun J P Liu J Am Chem
Soc 2007 129 7214
[4] C W Kim Y H Kim H G Cha D K Lee Y S Kang J NanosciNanotechnol 2006 6 3417
[5] Y W Jun J S Choi J Cheon Angew Chem Int Ed 2006 45 3414
[6] X Michalet F F Pinaud L A Bentolila J M Tsay S Doose J J Li G Sundaresan A M Wu
S S Gambhir S Weiss Science 2005 307 538
[7] M Hu J Y Chen Z Y Li L Au G V Hartland X D Li M Marquez Y N Xia Chem Soc
Rev 2006 35 1084
[8] M Vallet-Regi F Balas D Arcos Angew Chem Int Ed 2007 46 7548
[9] S T Selvan P K Patra C Y Ang J Y Ying Angew Chem Int Ed 2007 46 2448
[10] I S Lee N Lee J Park B H Kim Y W Yi T Kim T K Kim I H Lee S R Paik T
Hyeon J Am Chem Soc 2006 128 10658
[11] Y S Lin S H Wu Y Hung Y H Chou C Chang M L Lin C P Tsai C Y Mou Chem
Mater 2006 18 5170
[12] B H Wu H Zhang C Chen S C Lin N F Zheng Nano Res 2009 2 975
[13] J Kim J E Lee J Lee J H Yu B C Kim K An Y Hwang C Shin J G Park J Kim T
Hyeon J Am Chem Soc 2006 128 688
[14] S Santra H S Yang P H Holloway J T Stanley R A Mericle J Am Chem Soc 2005 127
1656
[15] D K Yi S T Selvan S S Lee G C Papaefthymiou D Kundaliya J Y Ying J Am Chem
Soc 2005 127 4990 65
[16] J H Gao B Zhang Y Gao Y Pan X X Zhang B Xu J Am Chem Soc 2007 129 11928 [17] J H Gao W Zhang P B Huang B Zhang X X Zhang B Xu J Am Chem Soc 2008 130 3710 [18] J H Gao G L Liang B Zhang Y Kuang X X Zhang B Xu J Am Chem Soc 2007 129 1428 [19] J H Gao G L Liang J S Cheung Y Pan Y Kuang F Zhao B Zhang X X Zhang E X Wu B Xu J Am Chem Soc 2008 130 11828 [20] A R Kortan R Hull R L Opila M G Bawendi M L Steigerwald P J Carroll L E Brus JAm Chem Soc 1990 112 1327 [21] X G Peng M C Schlamp A V Kadavanich A P Alivisatos J Am Chem Soc 1997 119 7019 [22] S Kim B Fisher H J Eisler M Bawendi J Am Chem Soc 2003 125 11466[23] H Zeng J Li Z L Wang J P Liu S Sun Nano Lett 2004 4 187 [24] H Zeng S Sun J Li Z L Wang J P Liu Appl Phys Lett 2004 85 792 [25] Z Xu Y Hou S Sun J Am Chem Soc 2007 129 8698 [26] Y H Lin N Kim J Q Wang C J Zhong J Phys Chem B 2005 109 21593 [27] C Wang H Daimon S Sun Nano Lett 2009 9 1493 [28] K W Kwon M Shim J Am Chem Soc 2005 127 10269 [29] H Yu M Chen P M Rice S X Wang R L White S Sun Nano Lett 2005 5 379 [30] J S Choi Y W Jun S I Yeon H C Kim J S Shin J Cheon J Am Chem Soc 2006 128 15982 [31] S H Choi H B Na Y I Park K An S G Kwon Y Jang M Park J Moon J S Son I CSong W K Moon T Hyeon J Am Chem Soc 2008 130 15573 [32] H W Gu R K Zheng X X Zhang B Xu J Am Chem Soc 2004 126 5664
66
The surface Hydrophobicity can be modified by
thiol compound
20
The surface self-assembling by thiol compound
21
GNP Application
Different bio sensor based self assemble design
22
23
The Sol-Gel Process is
a method for producing solid materials from small molecules
inn RT The method is used for the fabrication of metal oxides
especially the oxides of silicon and titanium
24
Alkoxide-sol-gel-method-preparation
25
HydrolysisCondensation
26
27
Why silica sol-gel coating
- Biocompatible
- Functionalization
- Physical trapping
- Chemical stability
- Physical stability
28
I Byun AW Coleman B Kim Micromech Microeng 23 (2013) 085016 29
Three Dimensional
Multi Dimensional matrix
One Dimensional
Layer by layer coatingSi
OR
ORRORO
Si
OR
RORO
NH2
Si
OR
RORO
SH
Si
OR
RORO O
O
n
Si
OR
RORO
HN
NH
NH2
Si
R
RRORO
30
Two Dimensional
Physical trapping in sol gel
31
32
apto ldquoto fitrdquo
mer ldquosmallest unit of repeating structurerdquo
What is an Aptamer
Oligonucleotide or Peptide
molecules that bind to a specific
target molecule
33
Types of Aptamer
- ssDNA
- dsDNA
- RNA
- Peptide
34
SELEX(Systematic Evolution of Ligands by Exponential Enrichment)
Selection of ligand sequences that bind to a target
partitioning of aptamers from
non-aptamers via affinity methods
amplification of bound aptamers
35
Targets of Aptamers
- Small Molecules
- Proteins
- Nucleic Acids
- Cells
- Tissues
- Organisms
lysozym Thrombin HIV TAR Hemin Interferon γ Vascular
endotherial growth factor (VEGF) Prostate specific antigen(PSA)
Dopamine hellip 36
37
MFMNP Design
Core Shell
Dumbbell Structure
Multicomponent Hybrid NP
38
39
Polymer Shell
Core Shell
Metal alloy
(FePt)
Magnetic nanolayer
(Fe3O4)
Thermal decomposition
of Fe(acac)3 on FePt
Magnetic NP
(Fe3O4)
Metal alloy
(Au Ag)
(Fe3O4) in Au3+ solution
at reduction condition
40
1 Core Shell structure
41
- Mix of NP during the growth of nanocrystals
- Sol-gel process
Core Shell synthesis
2 Dumbbell structures
42
- Mix of NP during the growth of nanocrystals
- Thermal rearrangement
- self-assemble at a liquid-liquid interface
- Functional sol-gel matrix
3 Multicomponent Hybrid NPs
Self-assembly processes provide an approach to
fabricating multicomponent hybrid NPs with
integrated multifunctionality
43
- Surfactant polymers
- Sol-gel process
44
Poly(lactico-co-glycolicacid)
(PLGA)
Poly(L-lysine)-PEG-folate
(PLL-PEG-FOL)
By mechanical
stirring at RT
in CH3Cl
45
46
W Lu SR Arumugam D Senapati ACSNANO 2010 4(3) 1739ndash1749
GNP GNP
GNP
RNA Aptamer
Anti-HER2C-reb-2 antibody
GNPCTAB
47
Magnetic nanoparticle surface modification Glucose oxidasse
(GOx)
PEGGPS
APSMNP
48Z Lin et al Analytica Chimica Acta 818 (2014) 54ndash60
49
P Sun H Zhang C Liu Langmuir 2010 26(2) 1278ndash1284 50
Immuno-labeling using Fe3O4CdTe nanocomposites which are formed by
linking multiple TGA-stabilized CdTe QDs with the thiol-functionalized silica
coated iron oxide nanoparticles
23-dimercaptosuccinic
acids (DMSA)Dopamine
Mercaptoundecanoic acid
(MUA)
HS COOH
4 Small multifunctional molecules
Glutaraldehyde 51
C J Xu K M Xu H W Gu R K Zheng H Liu X X Zhang Z H Guo B Xu J Am Chem Soc 2004 126 9938
Dopamine
Conditions (a) NaOH tert-butyl dicarbonate dioxane H2O 24 hrs (b) BnBr K2CO3 DMF
RT 24 h (c) 10 CF3COOH CH2Cl2 rt 5 h (d) succinic anhydride pyridine RTt 3 h (e)
NHS DCC DMAP CHCl3 RTt 3 h
NTA
52
3-mercapto trimethoxysilane
(MPS)
3-amino trimethoxysilane
(APS)
5 Silane multifunctional monomers
Glycidoxypropyltrimethoxy-silane
(GPS)
53
54
Multifunctional magnetic nanoparticles
application
Bioseparation
Biodetection(Real time monitoring)
Targeted drug delivery
Magnetic fluid Hyperthermia therapy
55
1 Specific Bio-Separation
bull Magnetic properties
bull Targeting agent
Cell organism molecule
56
His-tagged protein purification as
the first step towards recombinant
protein purification
58
Lysing Binding
Nickel
affinity
nanoparicle
His tag
fusion protein
Washing Elusion
Digestion
Binding
Nickel
affinity
nanoparticle
Washing Target
Recombinant
Protein
1- Preparation of Fe3O4SiO2Layered Double Hydroxide CoreminusShell
Microspheres for Magnetic Separation of Proteins J Am ChemSoc (2012)
18 134(2) 1071-7
Ni2+
2- Magnetic Nanocomposite Spheres Decorated with NiO Nanoparticles for a
Magnetically Recyclable Protein Separation System J Kim Y Piao N Lee Y
Park I-H Lee J-H Lee Adv Mater (2010) 22 57ndash60
His-tagged protein purification
Purification of His6-lipase using Fe3O4AundashLAndashIDAndashCu2+ NPs with various
amounts of NPs (lane 1 01 mg lane 2 02 mg lane 3
63
Synthetic procedure for the coreshell structure spheres
64
glutaraldehyde
(GLH)
Reference[1] H W Gu Z M Yang J H Gao C K Chang B Xu J Am Chem Soc 2005 127 34
[2] A Hutten D Sudfeld I Ennen G Reiss K Wojczykowski P Jutzi J Magn Magn Mater 2005
293 93
[3] G S Chaubey C Barcena N Poudyal C B Rong J M Gao S Sun J P Liu J Am Chem
Soc 2007 129 7214
[4] C W Kim Y H Kim H G Cha D K Lee Y S Kang J NanosciNanotechnol 2006 6 3417
[5] Y W Jun J S Choi J Cheon Angew Chem Int Ed 2006 45 3414
[6] X Michalet F F Pinaud L A Bentolila J M Tsay S Doose J J Li G Sundaresan A M Wu
S S Gambhir S Weiss Science 2005 307 538
[7] M Hu J Y Chen Z Y Li L Au G V Hartland X D Li M Marquez Y N Xia Chem Soc
Rev 2006 35 1084
[8] M Vallet-Regi F Balas D Arcos Angew Chem Int Ed 2007 46 7548
[9] S T Selvan P K Patra C Y Ang J Y Ying Angew Chem Int Ed 2007 46 2448
[10] I S Lee N Lee J Park B H Kim Y W Yi T Kim T K Kim I H Lee S R Paik T
Hyeon J Am Chem Soc 2006 128 10658
[11] Y S Lin S H Wu Y Hung Y H Chou C Chang M L Lin C P Tsai C Y Mou Chem
Mater 2006 18 5170
[12] B H Wu H Zhang C Chen S C Lin N F Zheng Nano Res 2009 2 975
[13] J Kim J E Lee J Lee J H Yu B C Kim K An Y Hwang C Shin J G Park J Kim T
Hyeon J Am Chem Soc 2006 128 688
[14] S Santra H S Yang P H Holloway J T Stanley R A Mericle J Am Chem Soc 2005 127
1656
[15] D K Yi S T Selvan S S Lee G C Papaefthymiou D Kundaliya J Y Ying J Am Chem
Soc 2005 127 4990 65
[16] J H Gao B Zhang Y Gao Y Pan X X Zhang B Xu J Am Chem Soc 2007 129 11928 [17] J H Gao W Zhang P B Huang B Zhang X X Zhang B Xu J Am Chem Soc 2008 130 3710 [18] J H Gao G L Liang B Zhang Y Kuang X X Zhang B Xu J Am Chem Soc 2007 129 1428 [19] J H Gao G L Liang J S Cheung Y Pan Y Kuang F Zhao B Zhang X X Zhang E X Wu B Xu J Am Chem Soc 2008 130 11828 [20] A R Kortan R Hull R L Opila M G Bawendi M L Steigerwald P J Carroll L E Brus JAm Chem Soc 1990 112 1327 [21] X G Peng M C Schlamp A V Kadavanich A P Alivisatos J Am Chem Soc 1997 119 7019 [22] S Kim B Fisher H J Eisler M Bawendi J Am Chem Soc 2003 125 11466[23] H Zeng J Li Z L Wang J P Liu S Sun Nano Lett 2004 4 187 [24] H Zeng S Sun J Li Z L Wang J P Liu Appl Phys Lett 2004 85 792 [25] Z Xu Y Hou S Sun J Am Chem Soc 2007 129 8698 [26] Y H Lin N Kim J Q Wang C J Zhong J Phys Chem B 2005 109 21593 [27] C Wang H Daimon S Sun Nano Lett 2009 9 1493 [28] K W Kwon M Shim J Am Chem Soc 2005 127 10269 [29] H Yu M Chen P M Rice S X Wang R L White S Sun Nano Lett 2005 5 379 [30] J S Choi Y W Jun S I Yeon H C Kim J S Shin J Cheon J Am Chem Soc 2006 128 15982 [31] S H Choi H B Na Y I Park K An S G Kwon Y Jang M Park J Moon J S Son I CSong W K Moon T Hyeon J Am Chem Soc 2008 130 15573 [32] H W Gu R K Zheng X X Zhang B Xu J Am Chem Soc 2004 126 5664
66
The surface self-assembling by thiol compound
21
GNP Application
Different bio sensor based self assemble design
22
23
The Sol-Gel Process is
a method for producing solid materials from small molecules
inn RT The method is used for the fabrication of metal oxides
especially the oxides of silicon and titanium
24
Alkoxide-sol-gel-method-preparation
25
HydrolysisCondensation
26
27
Why silica sol-gel coating
- Biocompatible
- Functionalization
- Physical trapping
- Chemical stability
- Physical stability
28
I Byun AW Coleman B Kim Micromech Microeng 23 (2013) 085016 29
Three Dimensional
Multi Dimensional matrix
One Dimensional
Layer by layer coatingSi
OR
ORRORO
Si
OR
RORO
NH2
Si
OR
RORO
SH
Si
OR
RORO O
O
n
Si
OR
RORO
HN
NH
NH2
Si
R
RRORO
30
Two Dimensional
Physical trapping in sol gel
31
32
apto ldquoto fitrdquo
mer ldquosmallest unit of repeating structurerdquo
What is an Aptamer
Oligonucleotide or Peptide
molecules that bind to a specific
target molecule
33
Types of Aptamer
- ssDNA
- dsDNA
- RNA
- Peptide
34
SELEX(Systematic Evolution of Ligands by Exponential Enrichment)
Selection of ligand sequences that bind to a target
partitioning of aptamers from
non-aptamers via affinity methods
amplification of bound aptamers
35
Targets of Aptamers
- Small Molecules
- Proteins
- Nucleic Acids
- Cells
- Tissues
- Organisms
lysozym Thrombin HIV TAR Hemin Interferon γ Vascular
endotherial growth factor (VEGF) Prostate specific antigen(PSA)
Dopamine hellip 36
37
MFMNP Design
Core Shell
Dumbbell Structure
Multicomponent Hybrid NP
38
39
Polymer Shell
Core Shell
Metal alloy
(FePt)
Magnetic nanolayer
(Fe3O4)
Thermal decomposition
of Fe(acac)3 on FePt
Magnetic NP
(Fe3O4)
Metal alloy
(Au Ag)
(Fe3O4) in Au3+ solution
at reduction condition
40
1 Core Shell structure
41
- Mix of NP during the growth of nanocrystals
- Sol-gel process
Core Shell synthesis
2 Dumbbell structures
42
- Mix of NP during the growth of nanocrystals
- Thermal rearrangement
- self-assemble at a liquid-liquid interface
- Functional sol-gel matrix
3 Multicomponent Hybrid NPs
Self-assembly processes provide an approach to
fabricating multicomponent hybrid NPs with
integrated multifunctionality
43
- Surfactant polymers
- Sol-gel process
44
Poly(lactico-co-glycolicacid)
(PLGA)
Poly(L-lysine)-PEG-folate
(PLL-PEG-FOL)
By mechanical
stirring at RT
in CH3Cl
45
46
W Lu SR Arumugam D Senapati ACSNANO 2010 4(3) 1739ndash1749
GNP GNP
GNP
RNA Aptamer
Anti-HER2C-reb-2 antibody
GNPCTAB
47
Magnetic nanoparticle surface modification Glucose oxidasse
(GOx)
PEGGPS
APSMNP
48Z Lin et al Analytica Chimica Acta 818 (2014) 54ndash60
49
P Sun H Zhang C Liu Langmuir 2010 26(2) 1278ndash1284 50
Immuno-labeling using Fe3O4CdTe nanocomposites which are formed by
linking multiple TGA-stabilized CdTe QDs with the thiol-functionalized silica
coated iron oxide nanoparticles
23-dimercaptosuccinic
acids (DMSA)Dopamine
Mercaptoundecanoic acid
(MUA)
HS COOH
4 Small multifunctional molecules
Glutaraldehyde 51
C J Xu K M Xu H W Gu R K Zheng H Liu X X Zhang Z H Guo B Xu J Am Chem Soc 2004 126 9938
Dopamine
Conditions (a) NaOH tert-butyl dicarbonate dioxane H2O 24 hrs (b) BnBr K2CO3 DMF
RT 24 h (c) 10 CF3COOH CH2Cl2 rt 5 h (d) succinic anhydride pyridine RTt 3 h (e)
NHS DCC DMAP CHCl3 RTt 3 h
NTA
52
3-mercapto trimethoxysilane
(MPS)
3-amino trimethoxysilane
(APS)
5 Silane multifunctional monomers
Glycidoxypropyltrimethoxy-silane
(GPS)
53
54
Multifunctional magnetic nanoparticles
application
Bioseparation
Biodetection(Real time monitoring)
Targeted drug delivery
Magnetic fluid Hyperthermia therapy
55
1 Specific Bio-Separation
bull Magnetic properties
bull Targeting agent
Cell organism molecule
56
His-tagged protein purification as
the first step towards recombinant
protein purification
58
Lysing Binding
Nickel
affinity
nanoparicle
His tag
fusion protein
Washing Elusion
Digestion
Binding
Nickel
affinity
nanoparticle
Washing Target
Recombinant
Protein
1- Preparation of Fe3O4SiO2Layered Double Hydroxide CoreminusShell
Microspheres for Magnetic Separation of Proteins J Am ChemSoc (2012)
18 134(2) 1071-7
Ni2+
2- Magnetic Nanocomposite Spheres Decorated with NiO Nanoparticles for a
Magnetically Recyclable Protein Separation System J Kim Y Piao N Lee Y
Park I-H Lee J-H Lee Adv Mater (2010) 22 57ndash60
His-tagged protein purification
Purification of His6-lipase using Fe3O4AundashLAndashIDAndashCu2+ NPs with various
amounts of NPs (lane 1 01 mg lane 2 02 mg lane 3
63
Synthetic procedure for the coreshell structure spheres
64
glutaraldehyde
(GLH)
Reference[1] H W Gu Z M Yang J H Gao C K Chang B Xu J Am Chem Soc 2005 127 34
[2] A Hutten D Sudfeld I Ennen G Reiss K Wojczykowski P Jutzi J Magn Magn Mater 2005
293 93
[3] G S Chaubey C Barcena N Poudyal C B Rong J M Gao S Sun J P Liu J Am Chem
Soc 2007 129 7214
[4] C W Kim Y H Kim H G Cha D K Lee Y S Kang J NanosciNanotechnol 2006 6 3417
[5] Y W Jun J S Choi J Cheon Angew Chem Int Ed 2006 45 3414
[6] X Michalet F F Pinaud L A Bentolila J M Tsay S Doose J J Li G Sundaresan A M Wu
S S Gambhir S Weiss Science 2005 307 538
[7] M Hu J Y Chen Z Y Li L Au G V Hartland X D Li M Marquez Y N Xia Chem Soc
Rev 2006 35 1084
[8] M Vallet-Regi F Balas D Arcos Angew Chem Int Ed 2007 46 7548
[9] S T Selvan P K Patra C Y Ang J Y Ying Angew Chem Int Ed 2007 46 2448
[10] I S Lee N Lee J Park B H Kim Y W Yi T Kim T K Kim I H Lee S R Paik T
Hyeon J Am Chem Soc 2006 128 10658
[11] Y S Lin S H Wu Y Hung Y H Chou C Chang M L Lin C P Tsai C Y Mou Chem
Mater 2006 18 5170
[12] B H Wu H Zhang C Chen S C Lin N F Zheng Nano Res 2009 2 975
[13] J Kim J E Lee J Lee J H Yu B C Kim K An Y Hwang C Shin J G Park J Kim T
Hyeon J Am Chem Soc 2006 128 688
[14] S Santra H S Yang P H Holloway J T Stanley R A Mericle J Am Chem Soc 2005 127
1656
[15] D K Yi S T Selvan S S Lee G C Papaefthymiou D Kundaliya J Y Ying J Am Chem
Soc 2005 127 4990 65
[16] J H Gao B Zhang Y Gao Y Pan X X Zhang B Xu J Am Chem Soc 2007 129 11928 [17] J H Gao W Zhang P B Huang B Zhang X X Zhang B Xu J Am Chem Soc 2008 130 3710 [18] J H Gao G L Liang B Zhang Y Kuang X X Zhang B Xu J Am Chem Soc 2007 129 1428 [19] J H Gao G L Liang J S Cheung Y Pan Y Kuang F Zhao B Zhang X X Zhang E X Wu B Xu J Am Chem Soc 2008 130 11828 [20] A R Kortan R Hull R L Opila M G Bawendi M L Steigerwald P J Carroll L E Brus JAm Chem Soc 1990 112 1327 [21] X G Peng M C Schlamp A V Kadavanich A P Alivisatos J Am Chem Soc 1997 119 7019 [22] S Kim B Fisher H J Eisler M Bawendi J Am Chem Soc 2003 125 11466[23] H Zeng J Li Z L Wang J P Liu S Sun Nano Lett 2004 4 187 [24] H Zeng S Sun J Li Z L Wang J P Liu Appl Phys Lett 2004 85 792 [25] Z Xu Y Hou S Sun J Am Chem Soc 2007 129 8698 [26] Y H Lin N Kim J Q Wang C J Zhong J Phys Chem B 2005 109 21593 [27] C Wang H Daimon S Sun Nano Lett 2009 9 1493 [28] K W Kwon M Shim J Am Chem Soc 2005 127 10269 [29] H Yu M Chen P M Rice S X Wang R L White S Sun Nano Lett 2005 5 379 [30] J S Choi Y W Jun S I Yeon H C Kim J S Shin J Cheon J Am Chem Soc 2006 128 15982 [31] S H Choi H B Na Y I Park K An S G Kwon Y Jang M Park J Moon J S Son I CSong W K Moon T Hyeon J Am Chem Soc 2008 130 15573 [32] H W Gu R K Zheng X X Zhang B Xu J Am Chem Soc 2004 126 5664
66
GNP Application
Different bio sensor based self assemble design
22
23
The Sol-Gel Process is
a method for producing solid materials from small molecules
inn RT The method is used for the fabrication of metal oxides
especially the oxides of silicon and titanium
24
Alkoxide-sol-gel-method-preparation
25
HydrolysisCondensation
26
27
Why silica sol-gel coating
- Biocompatible
- Functionalization
- Physical trapping
- Chemical stability
- Physical stability
28
I Byun AW Coleman B Kim Micromech Microeng 23 (2013) 085016 29
Three Dimensional
Multi Dimensional matrix
One Dimensional
Layer by layer coatingSi
OR
ORRORO
Si
OR
RORO
NH2
Si
OR
RORO
SH
Si
OR
RORO O
O
n
Si
OR
RORO
HN
NH
NH2
Si
R
RRORO
30
Two Dimensional
Physical trapping in sol gel
31
32
apto ldquoto fitrdquo
mer ldquosmallest unit of repeating structurerdquo
What is an Aptamer
Oligonucleotide or Peptide
molecules that bind to a specific
target molecule
33
Types of Aptamer
- ssDNA
- dsDNA
- RNA
- Peptide
34
SELEX(Systematic Evolution of Ligands by Exponential Enrichment)
Selection of ligand sequences that bind to a target
partitioning of aptamers from
non-aptamers via affinity methods
amplification of bound aptamers
35
Targets of Aptamers
- Small Molecules
- Proteins
- Nucleic Acids
- Cells
- Tissues
- Organisms
lysozym Thrombin HIV TAR Hemin Interferon γ Vascular
endotherial growth factor (VEGF) Prostate specific antigen(PSA)
Dopamine hellip 36
37
MFMNP Design
Core Shell
Dumbbell Structure
Multicomponent Hybrid NP
38
39
Polymer Shell
Core Shell
Metal alloy
(FePt)
Magnetic nanolayer
(Fe3O4)
Thermal decomposition
of Fe(acac)3 on FePt
Magnetic NP
(Fe3O4)
Metal alloy
(Au Ag)
(Fe3O4) in Au3+ solution
at reduction condition
40
1 Core Shell structure
41
- Mix of NP during the growth of nanocrystals
- Sol-gel process
Core Shell synthesis
2 Dumbbell structures
42
- Mix of NP during the growth of nanocrystals
- Thermal rearrangement
- self-assemble at a liquid-liquid interface
- Functional sol-gel matrix
3 Multicomponent Hybrid NPs
Self-assembly processes provide an approach to
fabricating multicomponent hybrid NPs with
integrated multifunctionality
43
- Surfactant polymers
- Sol-gel process
44
Poly(lactico-co-glycolicacid)
(PLGA)
Poly(L-lysine)-PEG-folate
(PLL-PEG-FOL)
By mechanical
stirring at RT
in CH3Cl
45
46
W Lu SR Arumugam D Senapati ACSNANO 2010 4(3) 1739ndash1749
GNP GNP
GNP
RNA Aptamer
Anti-HER2C-reb-2 antibody
GNPCTAB
47
Magnetic nanoparticle surface modification Glucose oxidasse
(GOx)
PEGGPS
APSMNP
48Z Lin et al Analytica Chimica Acta 818 (2014) 54ndash60
49
P Sun H Zhang C Liu Langmuir 2010 26(2) 1278ndash1284 50
Immuno-labeling using Fe3O4CdTe nanocomposites which are formed by
linking multiple TGA-stabilized CdTe QDs with the thiol-functionalized silica
coated iron oxide nanoparticles
23-dimercaptosuccinic
acids (DMSA)Dopamine
Mercaptoundecanoic acid
(MUA)
HS COOH
4 Small multifunctional molecules
Glutaraldehyde 51
C J Xu K M Xu H W Gu R K Zheng H Liu X X Zhang Z H Guo B Xu J Am Chem Soc 2004 126 9938
Dopamine
Conditions (a) NaOH tert-butyl dicarbonate dioxane H2O 24 hrs (b) BnBr K2CO3 DMF
RT 24 h (c) 10 CF3COOH CH2Cl2 rt 5 h (d) succinic anhydride pyridine RTt 3 h (e)
NHS DCC DMAP CHCl3 RTt 3 h
NTA
52
3-mercapto trimethoxysilane
(MPS)
3-amino trimethoxysilane
(APS)
5 Silane multifunctional monomers
Glycidoxypropyltrimethoxy-silane
(GPS)
53
54
Multifunctional magnetic nanoparticles
application
Bioseparation
Biodetection(Real time monitoring)
Targeted drug delivery
Magnetic fluid Hyperthermia therapy
55
1 Specific Bio-Separation
bull Magnetic properties
bull Targeting agent
Cell organism molecule
56
His-tagged protein purification as
the first step towards recombinant
protein purification
58
Lysing Binding
Nickel
affinity
nanoparicle
His tag
fusion protein
Washing Elusion
Digestion
Binding
Nickel
affinity
nanoparticle
Washing Target
Recombinant
Protein
1- Preparation of Fe3O4SiO2Layered Double Hydroxide CoreminusShell
Microspheres for Magnetic Separation of Proteins J Am ChemSoc (2012)
18 134(2) 1071-7
Ni2+
2- Magnetic Nanocomposite Spheres Decorated with NiO Nanoparticles for a
Magnetically Recyclable Protein Separation System J Kim Y Piao N Lee Y
Park I-H Lee J-H Lee Adv Mater (2010) 22 57ndash60
His-tagged protein purification
Purification of His6-lipase using Fe3O4AundashLAndashIDAndashCu2+ NPs with various
amounts of NPs (lane 1 01 mg lane 2 02 mg lane 3
63
Synthetic procedure for the coreshell structure spheres
64
glutaraldehyde
(GLH)
Reference[1] H W Gu Z M Yang J H Gao C K Chang B Xu J Am Chem Soc 2005 127 34
[2] A Hutten D Sudfeld I Ennen G Reiss K Wojczykowski P Jutzi J Magn Magn Mater 2005
293 93
[3] G S Chaubey C Barcena N Poudyal C B Rong J M Gao S Sun J P Liu J Am Chem
Soc 2007 129 7214
[4] C W Kim Y H Kim H G Cha D K Lee Y S Kang J NanosciNanotechnol 2006 6 3417
[5] Y W Jun J S Choi J Cheon Angew Chem Int Ed 2006 45 3414
[6] X Michalet F F Pinaud L A Bentolila J M Tsay S Doose J J Li G Sundaresan A M Wu
S S Gambhir S Weiss Science 2005 307 538
[7] M Hu J Y Chen Z Y Li L Au G V Hartland X D Li M Marquez Y N Xia Chem Soc
Rev 2006 35 1084
[8] M Vallet-Regi F Balas D Arcos Angew Chem Int Ed 2007 46 7548
[9] S T Selvan P K Patra C Y Ang J Y Ying Angew Chem Int Ed 2007 46 2448
[10] I S Lee N Lee J Park B H Kim Y W Yi T Kim T K Kim I H Lee S R Paik T
Hyeon J Am Chem Soc 2006 128 10658
[11] Y S Lin S H Wu Y Hung Y H Chou C Chang M L Lin C P Tsai C Y Mou Chem
Mater 2006 18 5170
[12] B H Wu H Zhang C Chen S C Lin N F Zheng Nano Res 2009 2 975
[13] J Kim J E Lee J Lee J H Yu B C Kim K An Y Hwang C Shin J G Park J Kim T
Hyeon J Am Chem Soc 2006 128 688
[14] S Santra H S Yang P H Holloway J T Stanley R A Mericle J Am Chem Soc 2005 127
1656
[15] D K Yi S T Selvan S S Lee G C Papaefthymiou D Kundaliya J Y Ying J Am Chem
Soc 2005 127 4990 65
[16] J H Gao B Zhang Y Gao Y Pan X X Zhang B Xu J Am Chem Soc 2007 129 11928 [17] J H Gao W Zhang P B Huang B Zhang X X Zhang B Xu J Am Chem Soc 2008 130 3710 [18] J H Gao G L Liang B Zhang Y Kuang X X Zhang B Xu J Am Chem Soc 2007 129 1428 [19] J H Gao G L Liang J S Cheung Y Pan Y Kuang F Zhao B Zhang X X Zhang E X Wu B Xu J Am Chem Soc 2008 130 11828 [20] A R Kortan R Hull R L Opila M G Bawendi M L Steigerwald P J Carroll L E Brus JAm Chem Soc 1990 112 1327 [21] X G Peng M C Schlamp A V Kadavanich A P Alivisatos J Am Chem Soc 1997 119 7019 [22] S Kim B Fisher H J Eisler M Bawendi J Am Chem Soc 2003 125 11466[23] H Zeng J Li Z L Wang J P Liu S Sun Nano Lett 2004 4 187 [24] H Zeng S Sun J Li Z L Wang J P Liu Appl Phys Lett 2004 85 792 [25] Z Xu Y Hou S Sun J Am Chem Soc 2007 129 8698 [26] Y H Lin N Kim J Q Wang C J Zhong J Phys Chem B 2005 109 21593 [27] C Wang H Daimon S Sun Nano Lett 2009 9 1493 [28] K W Kwon M Shim J Am Chem Soc 2005 127 10269 [29] H Yu M Chen P M Rice S X Wang R L White S Sun Nano Lett 2005 5 379 [30] J S Choi Y W Jun S I Yeon H C Kim J S Shin J Cheon J Am Chem Soc 2006 128 15982 [31] S H Choi H B Na Y I Park K An S G Kwon Y Jang M Park J Moon J S Son I CSong W K Moon T Hyeon J Am Chem Soc 2008 130 15573 [32] H W Gu R K Zheng X X Zhang B Xu J Am Chem Soc 2004 126 5664
66
23
The Sol-Gel Process is
a method for producing solid materials from small molecules
inn RT The method is used for the fabrication of metal oxides
especially the oxides of silicon and titanium
24
Alkoxide-sol-gel-method-preparation
25
HydrolysisCondensation
26
27
Why silica sol-gel coating
- Biocompatible
- Functionalization
- Physical trapping
- Chemical stability
- Physical stability
28
I Byun AW Coleman B Kim Micromech Microeng 23 (2013) 085016 29
Three Dimensional
Multi Dimensional matrix
One Dimensional
Layer by layer coatingSi
OR
ORRORO
Si
OR
RORO
NH2
Si
OR
RORO
SH
Si
OR
RORO O
O
n
Si
OR
RORO
HN
NH
NH2
Si
R
RRORO
30
Two Dimensional
Physical trapping in sol gel
31
32
apto ldquoto fitrdquo
mer ldquosmallest unit of repeating structurerdquo
What is an Aptamer
Oligonucleotide or Peptide
molecules that bind to a specific
target molecule
33
Types of Aptamer
- ssDNA
- dsDNA
- RNA
- Peptide
34
SELEX(Systematic Evolution of Ligands by Exponential Enrichment)
Selection of ligand sequences that bind to a target
partitioning of aptamers from
non-aptamers via affinity methods
amplification of bound aptamers
35
Targets of Aptamers
- Small Molecules
- Proteins
- Nucleic Acids
- Cells
- Tissues
- Organisms
lysozym Thrombin HIV TAR Hemin Interferon γ Vascular
endotherial growth factor (VEGF) Prostate specific antigen(PSA)
Dopamine hellip 36
37
MFMNP Design
Core Shell
Dumbbell Structure
Multicomponent Hybrid NP
38
39
Polymer Shell
Core Shell
Metal alloy
(FePt)
Magnetic nanolayer
(Fe3O4)
Thermal decomposition
of Fe(acac)3 on FePt
Magnetic NP
(Fe3O4)
Metal alloy
(Au Ag)
(Fe3O4) in Au3+ solution
at reduction condition
40
1 Core Shell structure
41
- Mix of NP during the growth of nanocrystals
- Sol-gel process
Core Shell synthesis
2 Dumbbell structures
42
- Mix of NP during the growth of nanocrystals
- Thermal rearrangement
- self-assemble at a liquid-liquid interface
- Functional sol-gel matrix
3 Multicomponent Hybrid NPs
Self-assembly processes provide an approach to
fabricating multicomponent hybrid NPs with
integrated multifunctionality
43
- Surfactant polymers
- Sol-gel process
44
Poly(lactico-co-glycolicacid)
(PLGA)
Poly(L-lysine)-PEG-folate
(PLL-PEG-FOL)
By mechanical
stirring at RT
in CH3Cl
45
46
W Lu SR Arumugam D Senapati ACSNANO 2010 4(3) 1739ndash1749
GNP GNP
GNP
RNA Aptamer
Anti-HER2C-reb-2 antibody
GNPCTAB
47
Magnetic nanoparticle surface modification Glucose oxidasse
(GOx)
PEGGPS
APSMNP
48Z Lin et al Analytica Chimica Acta 818 (2014) 54ndash60
49
P Sun H Zhang C Liu Langmuir 2010 26(2) 1278ndash1284 50
Immuno-labeling using Fe3O4CdTe nanocomposites which are formed by
linking multiple TGA-stabilized CdTe QDs with the thiol-functionalized silica
coated iron oxide nanoparticles
23-dimercaptosuccinic
acids (DMSA)Dopamine
Mercaptoundecanoic acid
(MUA)
HS COOH
4 Small multifunctional molecules
Glutaraldehyde 51
C J Xu K M Xu H W Gu R K Zheng H Liu X X Zhang Z H Guo B Xu J Am Chem Soc 2004 126 9938
Dopamine
Conditions (a) NaOH tert-butyl dicarbonate dioxane H2O 24 hrs (b) BnBr K2CO3 DMF
RT 24 h (c) 10 CF3COOH CH2Cl2 rt 5 h (d) succinic anhydride pyridine RTt 3 h (e)
NHS DCC DMAP CHCl3 RTt 3 h
NTA
52
3-mercapto trimethoxysilane
(MPS)
3-amino trimethoxysilane
(APS)
5 Silane multifunctional monomers
Glycidoxypropyltrimethoxy-silane
(GPS)
53
54
Multifunctional magnetic nanoparticles
application
Bioseparation
Biodetection(Real time monitoring)
Targeted drug delivery
Magnetic fluid Hyperthermia therapy
55
1 Specific Bio-Separation
bull Magnetic properties
bull Targeting agent
Cell organism molecule
56
His-tagged protein purification as
the first step towards recombinant
protein purification
58
Lysing Binding
Nickel
affinity
nanoparicle
His tag
fusion protein
Washing Elusion
Digestion
Binding
Nickel
affinity
nanoparticle
Washing Target
Recombinant
Protein
1- Preparation of Fe3O4SiO2Layered Double Hydroxide CoreminusShell
Microspheres for Magnetic Separation of Proteins J Am ChemSoc (2012)
18 134(2) 1071-7
Ni2+
2- Magnetic Nanocomposite Spheres Decorated with NiO Nanoparticles for a
Magnetically Recyclable Protein Separation System J Kim Y Piao N Lee Y
Park I-H Lee J-H Lee Adv Mater (2010) 22 57ndash60
His-tagged protein purification
Purification of His6-lipase using Fe3O4AundashLAndashIDAndashCu2+ NPs with various
amounts of NPs (lane 1 01 mg lane 2 02 mg lane 3
63
Synthetic procedure for the coreshell structure spheres
64
glutaraldehyde
(GLH)
Reference[1] H W Gu Z M Yang J H Gao C K Chang B Xu J Am Chem Soc 2005 127 34
[2] A Hutten D Sudfeld I Ennen G Reiss K Wojczykowski P Jutzi J Magn Magn Mater 2005
293 93
[3] G S Chaubey C Barcena N Poudyal C B Rong J M Gao S Sun J P Liu J Am Chem
Soc 2007 129 7214
[4] C W Kim Y H Kim H G Cha D K Lee Y S Kang J NanosciNanotechnol 2006 6 3417
[5] Y W Jun J S Choi J Cheon Angew Chem Int Ed 2006 45 3414
[6] X Michalet F F Pinaud L A Bentolila J M Tsay S Doose J J Li G Sundaresan A M Wu
S S Gambhir S Weiss Science 2005 307 538
[7] M Hu J Y Chen Z Y Li L Au G V Hartland X D Li M Marquez Y N Xia Chem Soc
Rev 2006 35 1084
[8] M Vallet-Regi F Balas D Arcos Angew Chem Int Ed 2007 46 7548
[9] S T Selvan P K Patra C Y Ang J Y Ying Angew Chem Int Ed 2007 46 2448
[10] I S Lee N Lee J Park B H Kim Y W Yi T Kim T K Kim I H Lee S R Paik T
Hyeon J Am Chem Soc 2006 128 10658
[11] Y S Lin S H Wu Y Hung Y H Chou C Chang M L Lin C P Tsai C Y Mou Chem
Mater 2006 18 5170
[12] B H Wu H Zhang C Chen S C Lin N F Zheng Nano Res 2009 2 975
[13] J Kim J E Lee J Lee J H Yu B C Kim K An Y Hwang C Shin J G Park J Kim T
Hyeon J Am Chem Soc 2006 128 688
[14] S Santra H S Yang P H Holloway J T Stanley R A Mericle J Am Chem Soc 2005 127
1656
[15] D K Yi S T Selvan S S Lee G C Papaefthymiou D Kundaliya J Y Ying J Am Chem
Soc 2005 127 4990 65
[16] J H Gao B Zhang Y Gao Y Pan X X Zhang B Xu J Am Chem Soc 2007 129 11928 [17] J H Gao W Zhang P B Huang B Zhang X X Zhang B Xu J Am Chem Soc 2008 130 3710 [18] J H Gao G L Liang B Zhang Y Kuang X X Zhang B Xu J Am Chem Soc 2007 129 1428 [19] J H Gao G L Liang J S Cheung Y Pan Y Kuang F Zhao B Zhang X X Zhang E X Wu B Xu J Am Chem Soc 2008 130 11828 [20] A R Kortan R Hull R L Opila M G Bawendi M L Steigerwald P J Carroll L E Brus JAm Chem Soc 1990 112 1327 [21] X G Peng M C Schlamp A V Kadavanich A P Alivisatos J Am Chem Soc 1997 119 7019 [22] S Kim B Fisher H J Eisler M Bawendi J Am Chem Soc 2003 125 11466[23] H Zeng J Li Z L Wang J P Liu S Sun Nano Lett 2004 4 187 [24] H Zeng S Sun J Li Z L Wang J P Liu Appl Phys Lett 2004 85 792 [25] Z Xu Y Hou S Sun J Am Chem Soc 2007 129 8698 [26] Y H Lin N Kim J Q Wang C J Zhong J Phys Chem B 2005 109 21593 [27] C Wang H Daimon S Sun Nano Lett 2009 9 1493 [28] K W Kwon M Shim J Am Chem Soc 2005 127 10269 [29] H Yu M Chen P M Rice S X Wang R L White S Sun Nano Lett 2005 5 379 [30] J S Choi Y W Jun S I Yeon H C Kim J S Shin J Cheon J Am Chem Soc 2006 128 15982 [31] S H Choi H B Na Y I Park K An S G Kwon Y Jang M Park J Moon J S Son I CSong W K Moon T Hyeon J Am Chem Soc 2008 130 15573 [32] H W Gu R K Zheng X X Zhang B Xu J Am Chem Soc 2004 126 5664
66
The Sol-Gel Process is
a method for producing solid materials from small molecules
inn RT The method is used for the fabrication of metal oxides
especially the oxides of silicon and titanium
24
Alkoxide-sol-gel-method-preparation
25
HydrolysisCondensation
26
27
Why silica sol-gel coating
- Biocompatible
- Functionalization
- Physical trapping
- Chemical stability
- Physical stability
28
I Byun AW Coleman B Kim Micromech Microeng 23 (2013) 085016 29
Three Dimensional
Multi Dimensional matrix
One Dimensional
Layer by layer coatingSi
OR
ORRORO
Si
OR
RORO
NH2
Si
OR
RORO
SH
Si
OR
RORO O
O
n
Si
OR
RORO
HN
NH
NH2
Si
R
RRORO
30
Two Dimensional
Physical trapping in sol gel
31
32
apto ldquoto fitrdquo
mer ldquosmallest unit of repeating structurerdquo
What is an Aptamer
Oligonucleotide or Peptide
molecules that bind to a specific
target molecule
33
Types of Aptamer
- ssDNA
- dsDNA
- RNA
- Peptide
34
SELEX(Systematic Evolution of Ligands by Exponential Enrichment)
Selection of ligand sequences that bind to a target
partitioning of aptamers from
non-aptamers via affinity methods
amplification of bound aptamers
35
Targets of Aptamers
- Small Molecules
- Proteins
- Nucleic Acids
- Cells
- Tissues
- Organisms
lysozym Thrombin HIV TAR Hemin Interferon γ Vascular
endotherial growth factor (VEGF) Prostate specific antigen(PSA)
Dopamine hellip 36
37
MFMNP Design
Core Shell
Dumbbell Structure
Multicomponent Hybrid NP
38
39
Polymer Shell
Core Shell
Metal alloy
(FePt)
Magnetic nanolayer
(Fe3O4)
Thermal decomposition
of Fe(acac)3 on FePt
Magnetic NP
(Fe3O4)
Metal alloy
(Au Ag)
(Fe3O4) in Au3+ solution
at reduction condition
40
1 Core Shell structure
41
- Mix of NP during the growth of nanocrystals
- Sol-gel process
Core Shell synthesis
2 Dumbbell structures
42
- Mix of NP during the growth of nanocrystals
- Thermal rearrangement
- self-assemble at a liquid-liquid interface
- Functional sol-gel matrix
3 Multicomponent Hybrid NPs
Self-assembly processes provide an approach to
fabricating multicomponent hybrid NPs with
integrated multifunctionality
43
- Surfactant polymers
- Sol-gel process
44
Poly(lactico-co-glycolicacid)
(PLGA)
Poly(L-lysine)-PEG-folate
(PLL-PEG-FOL)
By mechanical
stirring at RT
in CH3Cl
45
46
W Lu SR Arumugam D Senapati ACSNANO 2010 4(3) 1739ndash1749
GNP GNP
GNP
RNA Aptamer
Anti-HER2C-reb-2 antibody
GNPCTAB
47
Magnetic nanoparticle surface modification Glucose oxidasse
(GOx)
PEGGPS
APSMNP
48Z Lin et al Analytica Chimica Acta 818 (2014) 54ndash60
49
P Sun H Zhang C Liu Langmuir 2010 26(2) 1278ndash1284 50
Immuno-labeling using Fe3O4CdTe nanocomposites which are formed by
linking multiple TGA-stabilized CdTe QDs with the thiol-functionalized silica
coated iron oxide nanoparticles
23-dimercaptosuccinic
acids (DMSA)Dopamine
Mercaptoundecanoic acid
(MUA)
HS COOH
4 Small multifunctional molecules
Glutaraldehyde 51
C J Xu K M Xu H W Gu R K Zheng H Liu X X Zhang Z H Guo B Xu J Am Chem Soc 2004 126 9938
Dopamine
Conditions (a) NaOH tert-butyl dicarbonate dioxane H2O 24 hrs (b) BnBr K2CO3 DMF
RT 24 h (c) 10 CF3COOH CH2Cl2 rt 5 h (d) succinic anhydride pyridine RTt 3 h (e)
NHS DCC DMAP CHCl3 RTt 3 h
NTA
52
3-mercapto trimethoxysilane
(MPS)
3-amino trimethoxysilane
(APS)
5 Silane multifunctional monomers
Glycidoxypropyltrimethoxy-silane
(GPS)
53
54
Multifunctional magnetic nanoparticles
application
Bioseparation
Biodetection(Real time monitoring)
Targeted drug delivery
Magnetic fluid Hyperthermia therapy
55
1 Specific Bio-Separation
bull Magnetic properties
bull Targeting agent
Cell organism molecule
56
His-tagged protein purification as
the first step towards recombinant
protein purification
58
Lysing Binding
Nickel
affinity
nanoparicle
His tag
fusion protein
Washing Elusion
Digestion
Binding
Nickel
affinity
nanoparticle
Washing Target
Recombinant
Protein
1- Preparation of Fe3O4SiO2Layered Double Hydroxide CoreminusShell
Microspheres for Magnetic Separation of Proteins J Am ChemSoc (2012)
18 134(2) 1071-7
Ni2+
2- Magnetic Nanocomposite Spheres Decorated with NiO Nanoparticles for a
Magnetically Recyclable Protein Separation System J Kim Y Piao N Lee Y
Park I-H Lee J-H Lee Adv Mater (2010) 22 57ndash60
His-tagged protein purification
Purification of His6-lipase using Fe3O4AundashLAndashIDAndashCu2+ NPs with various
amounts of NPs (lane 1 01 mg lane 2 02 mg lane 3
63
Synthetic procedure for the coreshell structure spheres
64
glutaraldehyde
(GLH)
Reference[1] H W Gu Z M Yang J H Gao C K Chang B Xu J Am Chem Soc 2005 127 34
[2] A Hutten D Sudfeld I Ennen G Reiss K Wojczykowski P Jutzi J Magn Magn Mater 2005
293 93
[3] G S Chaubey C Barcena N Poudyal C B Rong J M Gao S Sun J P Liu J Am Chem
Soc 2007 129 7214
[4] C W Kim Y H Kim H G Cha D K Lee Y S Kang J NanosciNanotechnol 2006 6 3417
[5] Y W Jun J S Choi J Cheon Angew Chem Int Ed 2006 45 3414
[6] X Michalet F F Pinaud L A Bentolila J M Tsay S Doose J J Li G Sundaresan A M Wu
S S Gambhir S Weiss Science 2005 307 538
[7] M Hu J Y Chen Z Y Li L Au G V Hartland X D Li M Marquez Y N Xia Chem Soc
Rev 2006 35 1084
[8] M Vallet-Regi F Balas D Arcos Angew Chem Int Ed 2007 46 7548
[9] S T Selvan P K Patra C Y Ang J Y Ying Angew Chem Int Ed 2007 46 2448
[10] I S Lee N Lee J Park B H Kim Y W Yi T Kim T K Kim I H Lee S R Paik T
Hyeon J Am Chem Soc 2006 128 10658
[11] Y S Lin S H Wu Y Hung Y H Chou C Chang M L Lin C P Tsai C Y Mou Chem
Mater 2006 18 5170
[12] B H Wu H Zhang C Chen S C Lin N F Zheng Nano Res 2009 2 975
[13] J Kim J E Lee J Lee J H Yu B C Kim K An Y Hwang C Shin J G Park J Kim T
Hyeon J Am Chem Soc 2006 128 688
[14] S Santra H S Yang P H Holloway J T Stanley R A Mericle J Am Chem Soc 2005 127
1656
[15] D K Yi S T Selvan S S Lee G C Papaefthymiou D Kundaliya J Y Ying J Am Chem
Soc 2005 127 4990 65
[16] J H Gao B Zhang Y Gao Y Pan X X Zhang B Xu J Am Chem Soc 2007 129 11928 [17] J H Gao W Zhang P B Huang B Zhang X X Zhang B Xu J Am Chem Soc 2008 130 3710 [18] J H Gao G L Liang B Zhang Y Kuang X X Zhang B Xu J Am Chem Soc 2007 129 1428 [19] J H Gao G L Liang J S Cheung Y Pan Y Kuang F Zhao B Zhang X X Zhang E X Wu B Xu J Am Chem Soc 2008 130 11828 [20] A R Kortan R Hull R L Opila M G Bawendi M L Steigerwald P J Carroll L E Brus JAm Chem Soc 1990 112 1327 [21] X G Peng M C Schlamp A V Kadavanich A P Alivisatos J Am Chem Soc 1997 119 7019 [22] S Kim B Fisher H J Eisler M Bawendi J Am Chem Soc 2003 125 11466[23] H Zeng J Li Z L Wang J P Liu S Sun Nano Lett 2004 4 187 [24] H Zeng S Sun J Li Z L Wang J P Liu Appl Phys Lett 2004 85 792 [25] Z Xu Y Hou S Sun J Am Chem Soc 2007 129 8698 [26] Y H Lin N Kim J Q Wang C J Zhong J Phys Chem B 2005 109 21593 [27] C Wang H Daimon S Sun Nano Lett 2009 9 1493 [28] K W Kwon M Shim J Am Chem Soc 2005 127 10269 [29] H Yu M Chen P M Rice S X Wang R L White S Sun Nano Lett 2005 5 379 [30] J S Choi Y W Jun S I Yeon H C Kim J S Shin J Cheon J Am Chem Soc 2006 128 15982 [31] S H Choi H B Na Y I Park K An S G Kwon Y Jang M Park J Moon J S Son I CSong W K Moon T Hyeon J Am Chem Soc 2008 130 15573 [32] H W Gu R K Zheng X X Zhang B Xu J Am Chem Soc 2004 126 5664
66
Alkoxide-sol-gel-method-preparation
25
HydrolysisCondensation
26
27
Why silica sol-gel coating
- Biocompatible
- Functionalization
- Physical trapping
- Chemical stability
- Physical stability
28
I Byun AW Coleman B Kim Micromech Microeng 23 (2013) 085016 29
Three Dimensional
Multi Dimensional matrix
One Dimensional
Layer by layer coatingSi
OR
ORRORO
Si
OR
RORO
NH2
Si
OR
RORO
SH
Si
OR
RORO O
O
n
Si
OR
RORO
HN
NH
NH2
Si
R
RRORO
30
Two Dimensional
Physical trapping in sol gel
31
32
apto ldquoto fitrdquo
mer ldquosmallest unit of repeating structurerdquo
What is an Aptamer
Oligonucleotide or Peptide
molecules that bind to a specific
target molecule
33
Types of Aptamer
- ssDNA
- dsDNA
- RNA
- Peptide
34
SELEX(Systematic Evolution of Ligands by Exponential Enrichment)
Selection of ligand sequences that bind to a target
partitioning of aptamers from
non-aptamers via affinity methods
amplification of bound aptamers
35
Targets of Aptamers
- Small Molecules
- Proteins
- Nucleic Acids
- Cells
- Tissues
- Organisms
lysozym Thrombin HIV TAR Hemin Interferon γ Vascular
endotherial growth factor (VEGF) Prostate specific antigen(PSA)
Dopamine hellip 36
37
MFMNP Design
Core Shell
Dumbbell Structure
Multicomponent Hybrid NP
38
39
Polymer Shell
Core Shell
Metal alloy
(FePt)
Magnetic nanolayer
(Fe3O4)
Thermal decomposition
of Fe(acac)3 on FePt
Magnetic NP
(Fe3O4)
Metal alloy
(Au Ag)
(Fe3O4) in Au3+ solution
at reduction condition
40
1 Core Shell structure
41
- Mix of NP during the growth of nanocrystals
- Sol-gel process
Core Shell synthesis
2 Dumbbell structures
42
- Mix of NP during the growth of nanocrystals
- Thermal rearrangement
- self-assemble at a liquid-liquid interface
- Functional sol-gel matrix
3 Multicomponent Hybrid NPs
Self-assembly processes provide an approach to
fabricating multicomponent hybrid NPs with
integrated multifunctionality
43
- Surfactant polymers
- Sol-gel process
44
Poly(lactico-co-glycolicacid)
(PLGA)
Poly(L-lysine)-PEG-folate
(PLL-PEG-FOL)
By mechanical
stirring at RT
in CH3Cl
45
46
W Lu SR Arumugam D Senapati ACSNANO 2010 4(3) 1739ndash1749
GNP GNP
GNP
RNA Aptamer
Anti-HER2C-reb-2 antibody
GNPCTAB
47
Magnetic nanoparticle surface modification Glucose oxidasse
(GOx)
PEGGPS
APSMNP
48Z Lin et al Analytica Chimica Acta 818 (2014) 54ndash60
49
P Sun H Zhang C Liu Langmuir 2010 26(2) 1278ndash1284 50
Immuno-labeling using Fe3O4CdTe nanocomposites which are formed by
linking multiple TGA-stabilized CdTe QDs with the thiol-functionalized silica
coated iron oxide nanoparticles
23-dimercaptosuccinic
acids (DMSA)Dopamine
Mercaptoundecanoic acid
(MUA)
HS COOH
4 Small multifunctional molecules
Glutaraldehyde 51
C J Xu K M Xu H W Gu R K Zheng H Liu X X Zhang Z H Guo B Xu J Am Chem Soc 2004 126 9938
Dopamine
Conditions (a) NaOH tert-butyl dicarbonate dioxane H2O 24 hrs (b) BnBr K2CO3 DMF
RT 24 h (c) 10 CF3COOH CH2Cl2 rt 5 h (d) succinic anhydride pyridine RTt 3 h (e)
NHS DCC DMAP CHCl3 RTt 3 h
NTA
52
3-mercapto trimethoxysilane
(MPS)
3-amino trimethoxysilane
(APS)
5 Silane multifunctional monomers
Glycidoxypropyltrimethoxy-silane
(GPS)
53
54
Multifunctional magnetic nanoparticles
application
Bioseparation
Biodetection(Real time monitoring)
Targeted drug delivery
Magnetic fluid Hyperthermia therapy
55
1 Specific Bio-Separation
bull Magnetic properties
bull Targeting agent
Cell organism molecule
56
His-tagged protein purification as
the first step towards recombinant
protein purification
58
Lysing Binding
Nickel
affinity
nanoparicle
His tag
fusion protein
Washing Elusion
Digestion
Binding
Nickel
affinity
nanoparticle
Washing Target
Recombinant
Protein
1- Preparation of Fe3O4SiO2Layered Double Hydroxide CoreminusShell
Microspheres for Magnetic Separation of Proteins J Am ChemSoc (2012)
18 134(2) 1071-7
Ni2+
2- Magnetic Nanocomposite Spheres Decorated with NiO Nanoparticles for a
Magnetically Recyclable Protein Separation System J Kim Y Piao N Lee Y
Park I-H Lee J-H Lee Adv Mater (2010) 22 57ndash60
His-tagged protein purification
Purification of His6-lipase using Fe3O4AundashLAndashIDAndashCu2+ NPs with various
amounts of NPs (lane 1 01 mg lane 2 02 mg lane 3
63
Synthetic procedure for the coreshell structure spheres
64
glutaraldehyde
(GLH)
Reference[1] H W Gu Z M Yang J H Gao C K Chang B Xu J Am Chem Soc 2005 127 34
[2] A Hutten D Sudfeld I Ennen G Reiss K Wojczykowski P Jutzi J Magn Magn Mater 2005
293 93
[3] G S Chaubey C Barcena N Poudyal C B Rong J M Gao S Sun J P Liu J Am Chem
Soc 2007 129 7214
[4] C W Kim Y H Kim H G Cha D K Lee Y S Kang J NanosciNanotechnol 2006 6 3417
[5] Y W Jun J S Choi J Cheon Angew Chem Int Ed 2006 45 3414
[6] X Michalet F F Pinaud L A Bentolila J M Tsay S Doose J J Li G Sundaresan A M Wu
S S Gambhir S Weiss Science 2005 307 538
[7] M Hu J Y Chen Z Y Li L Au G V Hartland X D Li M Marquez Y N Xia Chem Soc
Rev 2006 35 1084
[8] M Vallet-Regi F Balas D Arcos Angew Chem Int Ed 2007 46 7548
[9] S T Selvan P K Patra C Y Ang J Y Ying Angew Chem Int Ed 2007 46 2448
[10] I S Lee N Lee J Park B H Kim Y W Yi T Kim T K Kim I H Lee S R Paik T
Hyeon J Am Chem Soc 2006 128 10658
[11] Y S Lin S H Wu Y Hung Y H Chou C Chang M L Lin C P Tsai C Y Mou Chem
Mater 2006 18 5170
[12] B H Wu H Zhang C Chen S C Lin N F Zheng Nano Res 2009 2 975
[13] J Kim J E Lee J Lee J H Yu B C Kim K An Y Hwang C Shin J G Park J Kim T
Hyeon J Am Chem Soc 2006 128 688
[14] S Santra H S Yang P H Holloway J T Stanley R A Mericle J Am Chem Soc 2005 127
1656
[15] D K Yi S T Selvan S S Lee G C Papaefthymiou D Kundaliya J Y Ying J Am Chem
Soc 2005 127 4990 65
[16] J H Gao B Zhang Y Gao Y Pan X X Zhang B Xu J Am Chem Soc 2007 129 11928 [17] J H Gao W Zhang P B Huang B Zhang X X Zhang B Xu J Am Chem Soc 2008 130 3710 [18] J H Gao G L Liang B Zhang Y Kuang X X Zhang B Xu J Am Chem Soc 2007 129 1428 [19] J H Gao G L Liang J S Cheung Y Pan Y Kuang F Zhao B Zhang X X Zhang E X Wu B Xu J Am Chem Soc 2008 130 11828 [20] A R Kortan R Hull R L Opila M G Bawendi M L Steigerwald P J Carroll L E Brus JAm Chem Soc 1990 112 1327 [21] X G Peng M C Schlamp A V Kadavanich A P Alivisatos J Am Chem Soc 1997 119 7019 [22] S Kim B Fisher H J Eisler M Bawendi J Am Chem Soc 2003 125 11466[23] H Zeng J Li Z L Wang J P Liu S Sun Nano Lett 2004 4 187 [24] H Zeng S Sun J Li Z L Wang J P Liu Appl Phys Lett 2004 85 792 [25] Z Xu Y Hou S Sun J Am Chem Soc 2007 129 8698 [26] Y H Lin N Kim J Q Wang C J Zhong J Phys Chem B 2005 109 21593 [27] C Wang H Daimon S Sun Nano Lett 2009 9 1493 [28] K W Kwon M Shim J Am Chem Soc 2005 127 10269 [29] H Yu M Chen P M Rice S X Wang R L White S Sun Nano Lett 2005 5 379 [30] J S Choi Y W Jun S I Yeon H C Kim J S Shin J Cheon J Am Chem Soc 2006 128 15982 [31] S H Choi H B Na Y I Park K An S G Kwon Y Jang M Park J Moon J S Son I CSong W K Moon T Hyeon J Am Chem Soc 2008 130 15573 [32] H W Gu R K Zheng X X Zhang B Xu J Am Chem Soc 2004 126 5664
66
HydrolysisCondensation
26
27
Why silica sol-gel coating
- Biocompatible
- Functionalization
- Physical trapping
- Chemical stability
- Physical stability
28
I Byun AW Coleman B Kim Micromech Microeng 23 (2013) 085016 29
Three Dimensional
Multi Dimensional matrix
One Dimensional
Layer by layer coatingSi
OR
ORRORO
Si
OR
RORO
NH2
Si
OR
RORO
SH
Si
OR
RORO O
O
n
Si
OR
RORO
HN
NH
NH2
Si
R
RRORO
30
Two Dimensional
Physical trapping in sol gel
31
32
apto ldquoto fitrdquo
mer ldquosmallest unit of repeating structurerdquo
What is an Aptamer
Oligonucleotide or Peptide
molecules that bind to a specific
target molecule
33
Types of Aptamer
- ssDNA
- dsDNA
- RNA
- Peptide
34
SELEX(Systematic Evolution of Ligands by Exponential Enrichment)
Selection of ligand sequences that bind to a target
partitioning of aptamers from
non-aptamers via affinity methods
amplification of bound aptamers
35
Targets of Aptamers
- Small Molecules
- Proteins
- Nucleic Acids
- Cells
- Tissues
- Organisms
lysozym Thrombin HIV TAR Hemin Interferon γ Vascular
endotherial growth factor (VEGF) Prostate specific antigen(PSA)
Dopamine hellip 36
37
MFMNP Design
Core Shell
Dumbbell Structure
Multicomponent Hybrid NP
38
39
Polymer Shell
Core Shell
Metal alloy
(FePt)
Magnetic nanolayer
(Fe3O4)
Thermal decomposition
of Fe(acac)3 on FePt
Magnetic NP
(Fe3O4)
Metal alloy
(Au Ag)
(Fe3O4) in Au3+ solution
at reduction condition
40
1 Core Shell structure
41
- Mix of NP during the growth of nanocrystals
- Sol-gel process
Core Shell synthesis
2 Dumbbell structures
42
- Mix of NP during the growth of nanocrystals
- Thermal rearrangement
- self-assemble at a liquid-liquid interface
- Functional sol-gel matrix
3 Multicomponent Hybrid NPs
Self-assembly processes provide an approach to
fabricating multicomponent hybrid NPs with
integrated multifunctionality
43
- Surfactant polymers
- Sol-gel process
44
Poly(lactico-co-glycolicacid)
(PLGA)
Poly(L-lysine)-PEG-folate
(PLL-PEG-FOL)
By mechanical
stirring at RT
in CH3Cl
45
46
W Lu SR Arumugam D Senapati ACSNANO 2010 4(3) 1739ndash1749
GNP GNP
GNP
RNA Aptamer
Anti-HER2C-reb-2 antibody
GNPCTAB
47
Magnetic nanoparticle surface modification Glucose oxidasse
(GOx)
PEGGPS
APSMNP
48Z Lin et al Analytica Chimica Acta 818 (2014) 54ndash60
49
P Sun H Zhang C Liu Langmuir 2010 26(2) 1278ndash1284 50
Immuno-labeling using Fe3O4CdTe nanocomposites which are formed by
linking multiple TGA-stabilized CdTe QDs with the thiol-functionalized silica
coated iron oxide nanoparticles
23-dimercaptosuccinic
acids (DMSA)Dopamine
Mercaptoundecanoic acid
(MUA)
HS COOH
4 Small multifunctional molecules
Glutaraldehyde 51
C J Xu K M Xu H W Gu R K Zheng H Liu X X Zhang Z H Guo B Xu J Am Chem Soc 2004 126 9938
Dopamine
Conditions (a) NaOH tert-butyl dicarbonate dioxane H2O 24 hrs (b) BnBr K2CO3 DMF
RT 24 h (c) 10 CF3COOH CH2Cl2 rt 5 h (d) succinic anhydride pyridine RTt 3 h (e)
NHS DCC DMAP CHCl3 RTt 3 h
NTA
52
3-mercapto trimethoxysilane
(MPS)
3-amino trimethoxysilane
(APS)
5 Silane multifunctional monomers
Glycidoxypropyltrimethoxy-silane
(GPS)
53
54
Multifunctional magnetic nanoparticles
application
Bioseparation
Biodetection(Real time monitoring)
Targeted drug delivery
Magnetic fluid Hyperthermia therapy
55
1 Specific Bio-Separation
bull Magnetic properties
bull Targeting agent
Cell organism molecule
56
His-tagged protein purification as
the first step towards recombinant
protein purification
58
Lysing Binding
Nickel
affinity
nanoparicle
His tag
fusion protein
Washing Elusion
Digestion
Binding
Nickel
affinity
nanoparticle
Washing Target
Recombinant
Protein
1- Preparation of Fe3O4SiO2Layered Double Hydroxide CoreminusShell
Microspheres for Magnetic Separation of Proteins J Am ChemSoc (2012)
18 134(2) 1071-7
Ni2+
2- Magnetic Nanocomposite Spheres Decorated with NiO Nanoparticles for a
Magnetically Recyclable Protein Separation System J Kim Y Piao N Lee Y
Park I-H Lee J-H Lee Adv Mater (2010) 22 57ndash60
His-tagged protein purification
Purification of His6-lipase using Fe3O4AundashLAndashIDAndashCu2+ NPs with various
amounts of NPs (lane 1 01 mg lane 2 02 mg lane 3
63
Synthetic procedure for the coreshell structure spheres
64
glutaraldehyde
(GLH)
Reference[1] H W Gu Z M Yang J H Gao C K Chang B Xu J Am Chem Soc 2005 127 34
[2] A Hutten D Sudfeld I Ennen G Reiss K Wojczykowski P Jutzi J Magn Magn Mater 2005
293 93
[3] G S Chaubey C Barcena N Poudyal C B Rong J M Gao S Sun J P Liu J Am Chem
Soc 2007 129 7214
[4] C W Kim Y H Kim H G Cha D K Lee Y S Kang J NanosciNanotechnol 2006 6 3417
[5] Y W Jun J S Choi J Cheon Angew Chem Int Ed 2006 45 3414
[6] X Michalet F F Pinaud L A Bentolila J M Tsay S Doose J J Li G Sundaresan A M Wu
S S Gambhir S Weiss Science 2005 307 538
[7] M Hu J Y Chen Z Y Li L Au G V Hartland X D Li M Marquez Y N Xia Chem Soc
Rev 2006 35 1084
[8] M Vallet-Regi F Balas D Arcos Angew Chem Int Ed 2007 46 7548
[9] S T Selvan P K Patra C Y Ang J Y Ying Angew Chem Int Ed 2007 46 2448
[10] I S Lee N Lee J Park B H Kim Y W Yi T Kim T K Kim I H Lee S R Paik T
Hyeon J Am Chem Soc 2006 128 10658
[11] Y S Lin S H Wu Y Hung Y H Chou C Chang M L Lin C P Tsai C Y Mou Chem
Mater 2006 18 5170
[12] B H Wu H Zhang C Chen S C Lin N F Zheng Nano Res 2009 2 975
[13] J Kim J E Lee J Lee J H Yu B C Kim K An Y Hwang C Shin J G Park J Kim T
Hyeon J Am Chem Soc 2006 128 688
[14] S Santra H S Yang P H Holloway J T Stanley R A Mericle J Am Chem Soc 2005 127
1656
[15] D K Yi S T Selvan S S Lee G C Papaefthymiou D Kundaliya J Y Ying J Am Chem
Soc 2005 127 4990 65
[16] J H Gao B Zhang Y Gao Y Pan X X Zhang B Xu J Am Chem Soc 2007 129 11928 [17] J H Gao W Zhang P B Huang B Zhang X X Zhang B Xu J Am Chem Soc 2008 130 3710 [18] J H Gao G L Liang B Zhang Y Kuang X X Zhang B Xu J Am Chem Soc 2007 129 1428 [19] J H Gao G L Liang J S Cheung Y Pan Y Kuang F Zhao B Zhang X X Zhang E X Wu B Xu J Am Chem Soc 2008 130 11828 [20] A R Kortan R Hull R L Opila M G Bawendi M L Steigerwald P J Carroll L E Brus JAm Chem Soc 1990 112 1327 [21] X G Peng M C Schlamp A V Kadavanich A P Alivisatos J Am Chem Soc 1997 119 7019 [22] S Kim B Fisher H J Eisler M Bawendi J Am Chem Soc 2003 125 11466[23] H Zeng J Li Z L Wang J P Liu S Sun Nano Lett 2004 4 187 [24] H Zeng S Sun J Li Z L Wang J P Liu Appl Phys Lett 2004 85 792 [25] Z Xu Y Hou S Sun J Am Chem Soc 2007 129 8698 [26] Y H Lin N Kim J Q Wang C J Zhong J Phys Chem B 2005 109 21593 [27] C Wang H Daimon S Sun Nano Lett 2009 9 1493 [28] K W Kwon M Shim J Am Chem Soc 2005 127 10269 [29] H Yu M Chen P M Rice S X Wang R L White S Sun Nano Lett 2005 5 379 [30] J S Choi Y W Jun S I Yeon H C Kim J S Shin J Cheon J Am Chem Soc 2006 128 15982 [31] S H Choi H B Na Y I Park K An S G Kwon Y Jang M Park J Moon J S Son I CSong W K Moon T Hyeon J Am Chem Soc 2008 130 15573 [32] H W Gu R K Zheng X X Zhang B Xu J Am Chem Soc 2004 126 5664
66
27
Why silica sol-gel coating
- Biocompatible
- Functionalization
- Physical trapping
- Chemical stability
- Physical stability
28
I Byun AW Coleman B Kim Micromech Microeng 23 (2013) 085016 29
Three Dimensional
Multi Dimensional matrix
One Dimensional
Layer by layer coatingSi
OR
ORRORO
Si
OR
RORO
NH2
Si
OR
RORO
SH
Si
OR
RORO O
O
n
Si
OR
RORO
HN
NH
NH2
Si
R
RRORO
30
Two Dimensional
Physical trapping in sol gel
31
32
apto ldquoto fitrdquo
mer ldquosmallest unit of repeating structurerdquo
What is an Aptamer
Oligonucleotide or Peptide
molecules that bind to a specific
target molecule
33
Types of Aptamer
- ssDNA
- dsDNA
- RNA
- Peptide
34
SELEX(Systematic Evolution of Ligands by Exponential Enrichment)
Selection of ligand sequences that bind to a target
partitioning of aptamers from
non-aptamers via affinity methods
amplification of bound aptamers
35
Targets of Aptamers
- Small Molecules
- Proteins
- Nucleic Acids
- Cells
- Tissues
- Organisms
lysozym Thrombin HIV TAR Hemin Interferon γ Vascular
endotherial growth factor (VEGF) Prostate specific antigen(PSA)
Dopamine hellip 36
37
MFMNP Design
Core Shell
Dumbbell Structure
Multicomponent Hybrid NP
38
39
Polymer Shell
Core Shell
Metal alloy
(FePt)
Magnetic nanolayer
(Fe3O4)
Thermal decomposition
of Fe(acac)3 on FePt
Magnetic NP
(Fe3O4)
Metal alloy
(Au Ag)
(Fe3O4) in Au3+ solution
at reduction condition
40
1 Core Shell structure
41
- Mix of NP during the growth of nanocrystals
- Sol-gel process
Core Shell synthesis
2 Dumbbell structures
42
- Mix of NP during the growth of nanocrystals
- Thermal rearrangement
- self-assemble at a liquid-liquid interface
- Functional sol-gel matrix
3 Multicomponent Hybrid NPs
Self-assembly processes provide an approach to
fabricating multicomponent hybrid NPs with
integrated multifunctionality
43
- Surfactant polymers
- Sol-gel process
44
Poly(lactico-co-glycolicacid)
(PLGA)
Poly(L-lysine)-PEG-folate
(PLL-PEG-FOL)
By mechanical
stirring at RT
in CH3Cl
45
46
W Lu SR Arumugam D Senapati ACSNANO 2010 4(3) 1739ndash1749
GNP GNP
GNP
RNA Aptamer
Anti-HER2C-reb-2 antibody
GNPCTAB
47
Magnetic nanoparticle surface modification Glucose oxidasse
(GOx)
PEGGPS
APSMNP
48Z Lin et al Analytica Chimica Acta 818 (2014) 54ndash60
49
P Sun H Zhang C Liu Langmuir 2010 26(2) 1278ndash1284 50
Immuno-labeling using Fe3O4CdTe nanocomposites which are formed by
linking multiple TGA-stabilized CdTe QDs with the thiol-functionalized silica
coated iron oxide nanoparticles
23-dimercaptosuccinic
acids (DMSA)Dopamine
Mercaptoundecanoic acid
(MUA)
HS COOH
4 Small multifunctional molecules
Glutaraldehyde 51
C J Xu K M Xu H W Gu R K Zheng H Liu X X Zhang Z H Guo B Xu J Am Chem Soc 2004 126 9938
Dopamine
Conditions (a) NaOH tert-butyl dicarbonate dioxane H2O 24 hrs (b) BnBr K2CO3 DMF
RT 24 h (c) 10 CF3COOH CH2Cl2 rt 5 h (d) succinic anhydride pyridine RTt 3 h (e)
NHS DCC DMAP CHCl3 RTt 3 h
NTA
52
3-mercapto trimethoxysilane
(MPS)
3-amino trimethoxysilane
(APS)
5 Silane multifunctional monomers
Glycidoxypropyltrimethoxy-silane
(GPS)
53
54
Multifunctional magnetic nanoparticles
application
Bioseparation
Biodetection(Real time monitoring)
Targeted drug delivery
Magnetic fluid Hyperthermia therapy
55
1 Specific Bio-Separation
bull Magnetic properties
bull Targeting agent
Cell organism molecule
56
His-tagged protein purification as
the first step towards recombinant
protein purification
58
Lysing Binding
Nickel
affinity
nanoparicle
His tag
fusion protein
Washing Elusion
Digestion
Binding
Nickel
affinity
nanoparticle
Washing Target
Recombinant
Protein
1- Preparation of Fe3O4SiO2Layered Double Hydroxide CoreminusShell
Microspheres for Magnetic Separation of Proteins J Am ChemSoc (2012)
18 134(2) 1071-7
Ni2+
2- Magnetic Nanocomposite Spheres Decorated with NiO Nanoparticles for a
Magnetically Recyclable Protein Separation System J Kim Y Piao N Lee Y
Park I-H Lee J-H Lee Adv Mater (2010) 22 57ndash60
His-tagged protein purification
Purification of His6-lipase using Fe3O4AundashLAndashIDAndashCu2+ NPs with various
amounts of NPs (lane 1 01 mg lane 2 02 mg lane 3
63
Synthetic procedure for the coreshell structure spheres
64
glutaraldehyde
(GLH)
Reference[1] H W Gu Z M Yang J H Gao C K Chang B Xu J Am Chem Soc 2005 127 34
[2] A Hutten D Sudfeld I Ennen G Reiss K Wojczykowski P Jutzi J Magn Magn Mater 2005
293 93
[3] G S Chaubey C Barcena N Poudyal C B Rong J M Gao S Sun J P Liu J Am Chem
Soc 2007 129 7214
[4] C W Kim Y H Kim H G Cha D K Lee Y S Kang J NanosciNanotechnol 2006 6 3417
[5] Y W Jun J S Choi J Cheon Angew Chem Int Ed 2006 45 3414
[6] X Michalet F F Pinaud L A Bentolila J M Tsay S Doose J J Li G Sundaresan A M Wu
S S Gambhir S Weiss Science 2005 307 538
[7] M Hu J Y Chen Z Y Li L Au G V Hartland X D Li M Marquez Y N Xia Chem Soc
Rev 2006 35 1084
[8] M Vallet-Regi F Balas D Arcos Angew Chem Int Ed 2007 46 7548
[9] S T Selvan P K Patra C Y Ang J Y Ying Angew Chem Int Ed 2007 46 2448
[10] I S Lee N Lee J Park B H Kim Y W Yi T Kim T K Kim I H Lee S R Paik T
Hyeon J Am Chem Soc 2006 128 10658
[11] Y S Lin S H Wu Y Hung Y H Chou C Chang M L Lin C P Tsai C Y Mou Chem
Mater 2006 18 5170
[12] B H Wu H Zhang C Chen S C Lin N F Zheng Nano Res 2009 2 975
[13] J Kim J E Lee J Lee J H Yu B C Kim K An Y Hwang C Shin J G Park J Kim T
Hyeon J Am Chem Soc 2006 128 688
[14] S Santra H S Yang P H Holloway J T Stanley R A Mericle J Am Chem Soc 2005 127
1656
[15] D K Yi S T Selvan S S Lee G C Papaefthymiou D Kundaliya J Y Ying J Am Chem
Soc 2005 127 4990 65
[16] J H Gao B Zhang Y Gao Y Pan X X Zhang B Xu J Am Chem Soc 2007 129 11928 [17] J H Gao W Zhang P B Huang B Zhang X X Zhang B Xu J Am Chem Soc 2008 130 3710 [18] J H Gao G L Liang B Zhang Y Kuang X X Zhang B Xu J Am Chem Soc 2007 129 1428 [19] J H Gao G L Liang J S Cheung Y Pan Y Kuang F Zhao B Zhang X X Zhang E X Wu B Xu J Am Chem Soc 2008 130 11828 [20] A R Kortan R Hull R L Opila M G Bawendi M L Steigerwald P J Carroll L E Brus JAm Chem Soc 1990 112 1327 [21] X G Peng M C Schlamp A V Kadavanich A P Alivisatos J Am Chem Soc 1997 119 7019 [22] S Kim B Fisher H J Eisler M Bawendi J Am Chem Soc 2003 125 11466[23] H Zeng J Li Z L Wang J P Liu S Sun Nano Lett 2004 4 187 [24] H Zeng S Sun J Li Z L Wang J P Liu Appl Phys Lett 2004 85 792 [25] Z Xu Y Hou S Sun J Am Chem Soc 2007 129 8698 [26] Y H Lin N Kim J Q Wang C J Zhong J Phys Chem B 2005 109 21593 [27] C Wang H Daimon S Sun Nano Lett 2009 9 1493 [28] K W Kwon M Shim J Am Chem Soc 2005 127 10269 [29] H Yu M Chen P M Rice S X Wang R L White S Sun Nano Lett 2005 5 379 [30] J S Choi Y W Jun S I Yeon H C Kim J S Shin J Cheon J Am Chem Soc 2006 128 15982 [31] S H Choi H B Na Y I Park K An S G Kwon Y Jang M Park J Moon J S Son I CSong W K Moon T Hyeon J Am Chem Soc 2008 130 15573 [32] H W Gu R K Zheng X X Zhang B Xu J Am Chem Soc 2004 126 5664
66
Why silica sol-gel coating
- Biocompatible
- Functionalization
- Physical trapping
- Chemical stability
- Physical stability
28
I Byun AW Coleman B Kim Micromech Microeng 23 (2013) 085016 29
Three Dimensional
Multi Dimensional matrix
One Dimensional
Layer by layer coatingSi
OR
ORRORO
Si
OR
RORO
NH2
Si
OR
RORO
SH
Si
OR
RORO O
O
n
Si
OR
RORO
HN
NH
NH2
Si
R
RRORO
30
Two Dimensional
Physical trapping in sol gel
31
32
apto ldquoto fitrdquo
mer ldquosmallest unit of repeating structurerdquo
What is an Aptamer
Oligonucleotide or Peptide
molecules that bind to a specific
target molecule
33
Types of Aptamer
- ssDNA
- dsDNA
- RNA
- Peptide
34
SELEX(Systematic Evolution of Ligands by Exponential Enrichment)
Selection of ligand sequences that bind to a target
partitioning of aptamers from
non-aptamers via affinity methods
amplification of bound aptamers
35
Targets of Aptamers
- Small Molecules
- Proteins
- Nucleic Acids
- Cells
- Tissues
- Organisms
lysozym Thrombin HIV TAR Hemin Interferon γ Vascular
endotherial growth factor (VEGF) Prostate specific antigen(PSA)
Dopamine hellip 36
37
MFMNP Design
Core Shell
Dumbbell Structure
Multicomponent Hybrid NP
38
39
Polymer Shell
Core Shell
Metal alloy
(FePt)
Magnetic nanolayer
(Fe3O4)
Thermal decomposition
of Fe(acac)3 on FePt
Magnetic NP
(Fe3O4)
Metal alloy
(Au Ag)
(Fe3O4) in Au3+ solution
at reduction condition
40
1 Core Shell structure
41
- Mix of NP during the growth of nanocrystals
- Sol-gel process
Core Shell synthesis
2 Dumbbell structures
42
- Mix of NP during the growth of nanocrystals
- Thermal rearrangement
- self-assemble at a liquid-liquid interface
- Functional sol-gel matrix
3 Multicomponent Hybrid NPs
Self-assembly processes provide an approach to
fabricating multicomponent hybrid NPs with
integrated multifunctionality
43
- Surfactant polymers
- Sol-gel process
44
Poly(lactico-co-glycolicacid)
(PLGA)
Poly(L-lysine)-PEG-folate
(PLL-PEG-FOL)
By mechanical
stirring at RT
in CH3Cl
45
46
W Lu SR Arumugam D Senapati ACSNANO 2010 4(3) 1739ndash1749
GNP GNP
GNP
RNA Aptamer
Anti-HER2C-reb-2 antibody
GNPCTAB
47
Magnetic nanoparticle surface modification Glucose oxidasse
(GOx)
PEGGPS
APSMNP
48Z Lin et al Analytica Chimica Acta 818 (2014) 54ndash60
49
P Sun H Zhang C Liu Langmuir 2010 26(2) 1278ndash1284 50
Immuno-labeling using Fe3O4CdTe nanocomposites which are formed by
linking multiple TGA-stabilized CdTe QDs with the thiol-functionalized silica
coated iron oxide nanoparticles
23-dimercaptosuccinic
acids (DMSA)Dopamine
Mercaptoundecanoic acid
(MUA)
HS COOH
4 Small multifunctional molecules
Glutaraldehyde 51
C J Xu K M Xu H W Gu R K Zheng H Liu X X Zhang Z H Guo B Xu J Am Chem Soc 2004 126 9938
Dopamine
Conditions (a) NaOH tert-butyl dicarbonate dioxane H2O 24 hrs (b) BnBr K2CO3 DMF
RT 24 h (c) 10 CF3COOH CH2Cl2 rt 5 h (d) succinic anhydride pyridine RTt 3 h (e)
NHS DCC DMAP CHCl3 RTt 3 h
NTA
52
3-mercapto trimethoxysilane
(MPS)
3-amino trimethoxysilane
(APS)
5 Silane multifunctional monomers
Glycidoxypropyltrimethoxy-silane
(GPS)
53
54
Multifunctional magnetic nanoparticles
application
Bioseparation
Biodetection(Real time monitoring)
Targeted drug delivery
Magnetic fluid Hyperthermia therapy
55
1 Specific Bio-Separation
bull Magnetic properties
bull Targeting agent
Cell organism molecule
56
His-tagged protein purification as
the first step towards recombinant
protein purification
58
Lysing Binding
Nickel
affinity
nanoparicle
His tag
fusion protein
Washing Elusion
Digestion
Binding
Nickel
affinity
nanoparticle
Washing Target
Recombinant
Protein
1- Preparation of Fe3O4SiO2Layered Double Hydroxide CoreminusShell
Microspheres for Magnetic Separation of Proteins J Am ChemSoc (2012)
18 134(2) 1071-7
Ni2+
2- Magnetic Nanocomposite Spheres Decorated with NiO Nanoparticles for a
Magnetically Recyclable Protein Separation System J Kim Y Piao N Lee Y
Park I-H Lee J-H Lee Adv Mater (2010) 22 57ndash60
His-tagged protein purification
Purification of His6-lipase using Fe3O4AundashLAndashIDAndashCu2+ NPs with various
amounts of NPs (lane 1 01 mg lane 2 02 mg lane 3
63
Synthetic procedure for the coreshell structure spheres
64
glutaraldehyde
(GLH)
Reference[1] H W Gu Z M Yang J H Gao C K Chang B Xu J Am Chem Soc 2005 127 34
[2] A Hutten D Sudfeld I Ennen G Reiss K Wojczykowski P Jutzi J Magn Magn Mater 2005
293 93
[3] G S Chaubey C Barcena N Poudyal C B Rong J M Gao S Sun J P Liu J Am Chem
Soc 2007 129 7214
[4] C W Kim Y H Kim H G Cha D K Lee Y S Kang J NanosciNanotechnol 2006 6 3417
[5] Y W Jun J S Choi J Cheon Angew Chem Int Ed 2006 45 3414
[6] X Michalet F F Pinaud L A Bentolila J M Tsay S Doose J J Li G Sundaresan A M Wu
S S Gambhir S Weiss Science 2005 307 538
[7] M Hu J Y Chen Z Y Li L Au G V Hartland X D Li M Marquez Y N Xia Chem Soc
Rev 2006 35 1084
[8] M Vallet-Regi F Balas D Arcos Angew Chem Int Ed 2007 46 7548
[9] S T Selvan P K Patra C Y Ang J Y Ying Angew Chem Int Ed 2007 46 2448
[10] I S Lee N Lee J Park B H Kim Y W Yi T Kim T K Kim I H Lee S R Paik T
Hyeon J Am Chem Soc 2006 128 10658
[11] Y S Lin S H Wu Y Hung Y H Chou C Chang M L Lin C P Tsai C Y Mou Chem
Mater 2006 18 5170
[12] B H Wu H Zhang C Chen S C Lin N F Zheng Nano Res 2009 2 975
[13] J Kim J E Lee J Lee J H Yu B C Kim K An Y Hwang C Shin J G Park J Kim T
Hyeon J Am Chem Soc 2006 128 688
[14] S Santra H S Yang P H Holloway J T Stanley R A Mericle J Am Chem Soc 2005 127
1656
[15] D K Yi S T Selvan S S Lee G C Papaefthymiou D Kundaliya J Y Ying J Am Chem
Soc 2005 127 4990 65
[16] J H Gao B Zhang Y Gao Y Pan X X Zhang B Xu J Am Chem Soc 2007 129 11928 [17] J H Gao W Zhang P B Huang B Zhang X X Zhang B Xu J Am Chem Soc 2008 130 3710 [18] J H Gao G L Liang B Zhang Y Kuang X X Zhang B Xu J Am Chem Soc 2007 129 1428 [19] J H Gao G L Liang J S Cheung Y Pan Y Kuang F Zhao B Zhang X X Zhang E X Wu B Xu J Am Chem Soc 2008 130 11828 [20] A R Kortan R Hull R L Opila M G Bawendi M L Steigerwald P J Carroll L E Brus JAm Chem Soc 1990 112 1327 [21] X G Peng M C Schlamp A V Kadavanich A P Alivisatos J Am Chem Soc 1997 119 7019 [22] S Kim B Fisher H J Eisler M Bawendi J Am Chem Soc 2003 125 11466[23] H Zeng J Li Z L Wang J P Liu S Sun Nano Lett 2004 4 187 [24] H Zeng S Sun J Li Z L Wang J P Liu Appl Phys Lett 2004 85 792 [25] Z Xu Y Hou S Sun J Am Chem Soc 2007 129 8698 [26] Y H Lin N Kim J Q Wang C J Zhong J Phys Chem B 2005 109 21593 [27] C Wang H Daimon S Sun Nano Lett 2009 9 1493 [28] K W Kwon M Shim J Am Chem Soc 2005 127 10269 [29] H Yu M Chen P M Rice S X Wang R L White S Sun Nano Lett 2005 5 379 [30] J S Choi Y W Jun S I Yeon H C Kim J S Shin J Cheon J Am Chem Soc 2006 128 15982 [31] S H Choi H B Na Y I Park K An S G Kwon Y Jang M Park J Moon J S Son I CSong W K Moon T Hyeon J Am Chem Soc 2008 130 15573 [32] H W Gu R K Zheng X X Zhang B Xu J Am Chem Soc 2004 126 5664
66
I Byun AW Coleman B Kim Micromech Microeng 23 (2013) 085016 29
Three Dimensional
Multi Dimensional matrix
One Dimensional
Layer by layer coatingSi
OR
ORRORO
Si
OR
RORO
NH2
Si
OR
RORO
SH
Si
OR
RORO O
O
n
Si
OR
RORO
HN
NH
NH2
Si
R
RRORO
30
Two Dimensional
Physical trapping in sol gel
31
32
apto ldquoto fitrdquo
mer ldquosmallest unit of repeating structurerdquo
What is an Aptamer
Oligonucleotide or Peptide
molecules that bind to a specific
target molecule
33
Types of Aptamer
- ssDNA
- dsDNA
- RNA
- Peptide
34
SELEX(Systematic Evolution of Ligands by Exponential Enrichment)
Selection of ligand sequences that bind to a target
partitioning of aptamers from
non-aptamers via affinity methods
amplification of bound aptamers
35
Targets of Aptamers
- Small Molecules
- Proteins
- Nucleic Acids
- Cells
- Tissues
- Organisms
lysozym Thrombin HIV TAR Hemin Interferon γ Vascular
endotherial growth factor (VEGF) Prostate specific antigen(PSA)
Dopamine hellip 36
37
MFMNP Design
Core Shell
Dumbbell Structure
Multicomponent Hybrid NP
38
39
Polymer Shell
Core Shell
Metal alloy
(FePt)
Magnetic nanolayer
(Fe3O4)
Thermal decomposition
of Fe(acac)3 on FePt
Magnetic NP
(Fe3O4)
Metal alloy
(Au Ag)
(Fe3O4) in Au3+ solution
at reduction condition
40
1 Core Shell structure
41
- Mix of NP during the growth of nanocrystals
- Sol-gel process
Core Shell synthesis
2 Dumbbell structures
42
- Mix of NP during the growth of nanocrystals
- Thermal rearrangement
- self-assemble at a liquid-liquid interface
- Functional sol-gel matrix
3 Multicomponent Hybrid NPs
Self-assembly processes provide an approach to
fabricating multicomponent hybrid NPs with
integrated multifunctionality
43
- Surfactant polymers
- Sol-gel process
44
Poly(lactico-co-glycolicacid)
(PLGA)
Poly(L-lysine)-PEG-folate
(PLL-PEG-FOL)
By mechanical
stirring at RT
in CH3Cl
45
46
W Lu SR Arumugam D Senapati ACSNANO 2010 4(3) 1739ndash1749
GNP GNP
GNP
RNA Aptamer
Anti-HER2C-reb-2 antibody
GNPCTAB
47
Magnetic nanoparticle surface modification Glucose oxidasse
(GOx)
PEGGPS
APSMNP
48Z Lin et al Analytica Chimica Acta 818 (2014) 54ndash60
49
P Sun H Zhang C Liu Langmuir 2010 26(2) 1278ndash1284 50
Immuno-labeling using Fe3O4CdTe nanocomposites which are formed by
linking multiple TGA-stabilized CdTe QDs with the thiol-functionalized silica
coated iron oxide nanoparticles
23-dimercaptosuccinic
acids (DMSA)Dopamine
Mercaptoundecanoic acid
(MUA)
HS COOH
4 Small multifunctional molecules
Glutaraldehyde 51
C J Xu K M Xu H W Gu R K Zheng H Liu X X Zhang Z H Guo B Xu J Am Chem Soc 2004 126 9938
Dopamine
Conditions (a) NaOH tert-butyl dicarbonate dioxane H2O 24 hrs (b) BnBr K2CO3 DMF
RT 24 h (c) 10 CF3COOH CH2Cl2 rt 5 h (d) succinic anhydride pyridine RTt 3 h (e)
NHS DCC DMAP CHCl3 RTt 3 h
NTA
52
3-mercapto trimethoxysilane
(MPS)
3-amino trimethoxysilane
(APS)
5 Silane multifunctional monomers
Glycidoxypropyltrimethoxy-silane
(GPS)
53
54
Multifunctional magnetic nanoparticles
application
Bioseparation
Biodetection(Real time monitoring)
Targeted drug delivery
Magnetic fluid Hyperthermia therapy
55
1 Specific Bio-Separation
bull Magnetic properties
bull Targeting agent
Cell organism molecule
56
His-tagged protein purification as
the first step towards recombinant
protein purification
58
Lysing Binding
Nickel
affinity
nanoparicle
His tag
fusion protein
Washing Elusion
Digestion
Binding
Nickel
affinity
nanoparticle
Washing Target
Recombinant
Protein
1- Preparation of Fe3O4SiO2Layered Double Hydroxide CoreminusShell
Microspheres for Magnetic Separation of Proteins J Am ChemSoc (2012)
18 134(2) 1071-7
Ni2+
2- Magnetic Nanocomposite Spheres Decorated with NiO Nanoparticles for a
Magnetically Recyclable Protein Separation System J Kim Y Piao N Lee Y
Park I-H Lee J-H Lee Adv Mater (2010) 22 57ndash60
His-tagged protein purification
Purification of His6-lipase using Fe3O4AundashLAndashIDAndashCu2+ NPs with various
amounts of NPs (lane 1 01 mg lane 2 02 mg lane 3
63
Synthetic procedure for the coreshell structure spheres
64
glutaraldehyde
(GLH)
Reference[1] H W Gu Z M Yang J H Gao C K Chang B Xu J Am Chem Soc 2005 127 34
[2] A Hutten D Sudfeld I Ennen G Reiss K Wojczykowski P Jutzi J Magn Magn Mater 2005
293 93
[3] G S Chaubey C Barcena N Poudyal C B Rong J M Gao S Sun J P Liu J Am Chem
Soc 2007 129 7214
[4] C W Kim Y H Kim H G Cha D K Lee Y S Kang J NanosciNanotechnol 2006 6 3417
[5] Y W Jun J S Choi J Cheon Angew Chem Int Ed 2006 45 3414
[6] X Michalet F F Pinaud L A Bentolila J M Tsay S Doose J J Li G Sundaresan A M Wu
S S Gambhir S Weiss Science 2005 307 538
[7] M Hu J Y Chen Z Y Li L Au G V Hartland X D Li M Marquez Y N Xia Chem Soc
Rev 2006 35 1084
[8] M Vallet-Regi F Balas D Arcos Angew Chem Int Ed 2007 46 7548
[9] S T Selvan P K Patra C Y Ang J Y Ying Angew Chem Int Ed 2007 46 2448
[10] I S Lee N Lee J Park B H Kim Y W Yi T Kim T K Kim I H Lee S R Paik T
Hyeon J Am Chem Soc 2006 128 10658
[11] Y S Lin S H Wu Y Hung Y H Chou C Chang M L Lin C P Tsai C Y Mou Chem
Mater 2006 18 5170
[12] B H Wu H Zhang C Chen S C Lin N F Zheng Nano Res 2009 2 975
[13] J Kim J E Lee J Lee J H Yu B C Kim K An Y Hwang C Shin J G Park J Kim T
Hyeon J Am Chem Soc 2006 128 688
[14] S Santra H S Yang P H Holloway J T Stanley R A Mericle J Am Chem Soc 2005 127
1656
[15] D K Yi S T Selvan S S Lee G C Papaefthymiou D Kundaliya J Y Ying J Am Chem
Soc 2005 127 4990 65
[16] J H Gao B Zhang Y Gao Y Pan X X Zhang B Xu J Am Chem Soc 2007 129 11928 [17] J H Gao W Zhang P B Huang B Zhang X X Zhang B Xu J Am Chem Soc 2008 130 3710 [18] J H Gao G L Liang B Zhang Y Kuang X X Zhang B Xu J Am Chem Soc 2007 129 1428 [19] J H Gao G L Liang J S Cheung Y Pan Y Kuang F Zhao B Zhang X X Zhang E X Wu B Xu J Am Chem Soc 2008 130 11828 [20] A R Kortan R Hull R L Opila M G Bawendi M L Steigerwald P J Carroll L E Brus JAm Chem Soc 1990 112 1327 [21] X G Peng M C Schlamp A V Kadavanich A P Alivisatos J Am Chem Soc 1997 119 7019 [22] S Kim B Fisher H J Eisler M Bawendi J Am Chem Soc 2003 125 11466[23] H Zeng J Li Z L Wang J P Liu S Sun Nano Lett 2004 4 187 [24] H Zeng S Sun J Li Z L Wang J P Liu Appl Phys Lett 2004 85 792 [25] Z Xu Y Hou S Sun J Am Chem Soc 2007 129 8698 [26] Y H Lin N Kim J Q Wang C J Zhong J Phys Chem B 2005 109 21593 [27] C Wang H Daimon S Sun Nano Lett 2009 9 1493 [28] K W Kwon M Shim J Am Chem Soc 2005 127 10269 [29] H Yu M Chen P M Rice S X Wang R L White S Sun Nano Lett 2005 5 379 [30] J S Choi Y W Jun S I Yeon H C Kim J S Shin J Cheon J Am Chem Soc 2006 128 15982 [31] S H Choi H B Na Y I Park K An S G Kwon Y Jang M Park J Moon J S Son I CSong W K Moon T Hyeon J Am Chem Soc 2008 130 15573 [32] H W Gu R K Zheng X X Zhang B Xu J Am Chem Soc 2004 126 5664
66
Three Dimensional
Multi Dimensional matrix
One Dimensional
Layer by layer coatingSi
OR
ORRORO
Si
OR
RORO
NH2
Si
OR
RORO
SH
Si
OR
RORO O
O
n
Si
OR
RORO
HN
NH
NH2
Si
R
RRORO
30
Two Dimensional
Physical trapping in sol gel
31
32
apto ldquoto fitrdquo
mer ldquosmallest unit of repeating structurerdquo
What is an Aptamer
Oligonucleotide or Peptide
molecules that bind to a specific
target molecule
33
Types of Aptamer
- ssDNA
- dsDNA
- RNA
- Peptide
34
SELEX(Systematic Evolution of Ligands by Exponential Enrichment)
Selection of ligand sequences that bind to a target
partitioning of aptamers from
non-aptamers via affinity methods
amplification of bound aptamers
35
Targets of Aptamers
- Small Molecules
- Proteins
- Nucleic Acids
- Cells
- Tissues
- Organisms
lysozym Thrombin HIV TAR Hemin Interferon γ Vascular
endotherial growth factor (VEGF) Prostate specific antigen(PSA)
Dopamine hellip 36
37
MFMNP Design
Core Shell
Dumbbell Structure
Multicomponent Hybrid NP
38
39
Polymer Shell
Core Shell
Metal alloy
(FePt)
Magnetic nanolayer
(Fe3O4)
Thermal decomposition
of Fe(acac)3 on FePt
Magnetic NP
(Fe3O4)
Metal alloy
(Au Ag)
(Fe3O4) in Au3+ solution
at reduction condition
40
1 Core Shell structure
41
- Mix of NP during the growth of nanocrystals
- Sol-gel process
Core Shell synthesis
2 Dumbbell structures
42
- Mix of NP during the growth of nanocrystals
- Thermal rearrangement
- self-assemble at a liquid-liquid interface
- Functional sol-gel matrix
3 Multicomponent Hybrid NPs
Self-assembly processes provide an approach to
fabricating multicomponent hybrid NPs with
integrated multifunctionality
43
- Surfactant polymers
- Sol-gel process
44
Poly(lactico-co-glycolicacid)
(PLGA)
Poly(L-lysine)-PEG-folate
(PLL-PEG-FOL)
By mechanical
stirring at RT
in CH3Cl
45
46
W Lu SR Arumugam D Senapati ACSNANO 2010 4(3) 1739ndash1749
GNP GNP
GNP
RNA Aptamer
Anti-HER2C-reb-2 antibody
GNPCTAB
47
Magnetic nanoparticle surface modification Glucose oxidasse
(GOx)
PEGGPS
APSMNP
48Z Lin et al Analytica Chimica Acta 818 (2014) 54ndash60
49
P Sun H Zhang C Liu Langmuir 2010 26(2) 1278ndash1284 50
Immuno-labeling using Fe3O4CdTe nanocomposites which are formed by
linking multiple TGA-stabilized CdTe QDs with the thiol-functionalized silica
coated iron oxide nanoparticles
23-dimercaptosuccinic
acids (DMSA)Dopamine
Mercaptoundecanoic acid
(MUA)
HS COOH
4 Small multifunctional molecules
Glutaraldehyde 51
C J Xu K M Xu H W Gu R K Zheng H Liu X X Zhang Z H Guo B Xu J Am Chem Soc 2004 126 9938
Dopamine
Conditions (a) NaOH tert-butyl dicarbonate dioxane H2O 24 hrs (b) BnBr K2CO3 DMF
RT 24 h (c) 10 CF3COOH CH2Cl2 rt 5 h (d) succinic anhydride pyridine RTt 3 h (e)
NHS DCC DMAP CHCl3 RTt 3 h
NTA
52
3-mercapto trimethoxysilane
(MPS)
3-amino trimethoxysilane
(APS)
5 Silane multifunctional monomers
Glycidoxypropyltrimethoxy-silane
(GPS)
53
54
Multifunctional magnetic nanoparticles
application
Bioseparation
Biodetection(Real time monitoring)
Targeted drug delivery
Magnetic fluid Hyperthermia therapy
55
1 Specific Bio-Separation
bull Magnetic properties
bull Targeting agent
Cell organism molecule
56
His-tagged protein purification as
the first step towards recombinant
protein purification
58
Lysing Binding
Nickel
affinity
nanoparicle
His tag
fusion protein
Washing Elusion
Digestion
Binding
Nickel
affinity
nanoparticle
Washing Target
Recombinant
Protein
1- Preparation of Fe3O4SiO2Layered Double Hydroxide CoreminusShell
Microspheres for Magnetic Separation of Proteins J Am ChemSoc (2012)
18 134(2) 1071-7
Ni2+
2- Magnetic Nanocomposite Spheres Decorated with NiO Nanoparticles for a
Magnetically Recyclable Protein Separation System J Kim Y Piao N Lee Y
Park I-H Lee J-H Lee Adv Mater (2010) 22 57ndash60
His-tagged protein purification
Purification of His6-lipase using Fe3O4AundashLAndashIDAndashCu2+ NPs with various
amounts of NPs (lane 1 01 mg lane 2 02 mg lane 3
63
Synthetic procedure for the coreshell structure spheres
64
glutaraldehyde
(GLH)
Reference[1] H W Gu Z M Yang J H Gao C K Chang B Xu J Am Chem Soc 2005 127 34
[2] A Hutten D Sudfeld I Ennen G Reiss K Wojczykowski P Jutzi J Magn Magn Mater 2005
293 93
[3] G S Chaubey C Barcena N Poudyal C B Rong J M Gao S Sun J P Liu J Am Chem
Soc 2007 129 7214
[4] C W Kim Y H Kim H G Cha D K Lee Y S Kang J NanosciNanotechnol 2006 6 3417
[5] Y W Jun J S Choi J Cheon Angew Chem Int Ed 2006 45 3414
[6] X Michalet F F Pinaud L A Bentolila J M Tsay S Doose J J Li G Sundaresan A M Wu
S S Gambhir S Weiss Science 2005 307 538
[7] M Hu J Y Chen Z Y Li L Au G V Hartland X D Li M Marquez Y N Xia Chem Soc
Rev 2006 35 1084
[8] M Vallet-Regi F Balas D Arcos Angew Chem Int Ed 2007 46 7548
[9] S T Selvan P K Patra C Y Ang J Y Ying Angew Chem Int Ed 2007 46 2448
[10] I S Lee N Lee J Park B H Kim Y W Yi T Kim T K Kim I H Lee S R Paik T
Hyeon J Am Chem Soc 2006 128 10658
[11] Y S Lin S H Wu Y Hung Y H Chou C Chang M L Lin C P Tsai C Y Mou Chem
Mater 2006 18 5170
[12] B H Wu H Zhang C Chen S C Lin N F Zheng Nano Res 2009 2 975
[13] J Kim J E Lee J Lee J H Yu B C Kim K An Y Hwang C Shin J G Park J Kim T
Hyeon J Am Chem Soc 2006 128 688
[14] S Santra H S Yang P H Holloway J T Stanley R A Mericle J Am Chem Soc 2005 127
1656
[15] D K Yi S T Selvan S S Lee G C Papaefthymiou D Kundaliya J Y Ying J Am Chem
Soc 2005 127 4990 65
[16] J H Gao B Zhang Y Gao Y Pan X X Zhang B Xu J Am Chem Soc 2007 129 11928 [17] J H Gao W Zhang P B Huang B Zhang X X Zhang B Xu J Am Chem Soc 2008 130 3710 [18] J H Gao G L Liang B Zhang Y Kuang X X Zhang B Xu J Am Chem Soc 2007 129 1428 [19] J H Gao G L Liang J S Cheung Y Pan Y Kuang F Zhao B Zhang X X Zhang E X Wu B Xu J Am Chem Soc 2008 130 11828 [20] A R Kortan R Hull R L Opila M G Bawendi M L Steigerwald P J Carroll L E Brus JAm Chem Soc 1990 112 1327 [21] X G Peng M C Schlamp A V Kadavanich A P Alivisatos J Am Chem Soc 1997 119 7019 [22] S Kim B Fisher H J Eisler M Bawendi J Am Chem Soc 2003 125 11466[23] H Zeng J Li Z L Wang J P Liu S Sun Nano Lett 2004 4 187 [24] H Zeng S Sun J Li Z L Wang J P Liu Appl Phys Lett 2004 85 792 [25] Z Xu Y Hou S Sun J Am Chem Soc 2007 129 8698 [26] Y H Lin N Kim J Q Wang C J Zhong J Phys Chem B 2005 109 21593 [27] C Wang H Daimon S Sun Nano Lett 2009 9 1493 [28] K W Kwon M Shim J Am Chem Soc 2005 127 10269 [29] H Yu M Chen P M Rice S X Wang R L White S Sun Nano Lett 2005 5 379 [30] J S Choi Y W Jun S I Yeon H C Kim J S Shin J Cheon J Am Chem Soc 2006 128 15982 [31] S H Choi H B Na Y I Park K An S G Kwon Y Jang M Park J Moon J S Son I CSong W K Moon T Hyeon J Am Chem Soc 2008 130 15573 [32] H W Gu R K Zheng X X Zhang B Xu J Am Chem Soc 2004 126 5664
66
Physical trapping in sol gel
31
32
apto ldquoto fitrdquo
mer ldquosmallest unit of repeating structurerdquo
What is an Aptamer
Oligonucleotide or Peptide
molecules that bind to a specific
target molecule
33
Types of Aptamer
- ssDNA
- dsDNA
- RNA
- Peptide
34
SELEX(Systematic Evolution of Ligands by Exponential Enrichment)
Selection of ligand sequences that bind to a target
partitioning of aptamers from
non-aptamers via affinity methods
amplification of bound aptamers
35
Targets of Aptamers
- Small Molecules
- Proteins
- Nucleic Acids
- Cells
- Tissues
- Organisms
lysozym Thrombin HIV TAR Hemin Interferon γ Vascular
endotherial growth factor (VEGF) Prostate specific antigen(PSA)
Dopamine hellip 36
37
MFMNP Design
Core Shell
Dumbbell Structure
Multicomponent Hybrid NP
38
39
Polymer Shell
Core Shell
Metal alloy
(FePt)
Magnetic nanolayer
(Fe3O4)
Thermal decomposition
of Fe(acac)3 on FePt
Magnetic NP
(Fe3O4)
Metal alloy
(Au Ag)
(Fe3O4) in Au3+ solution
at reduction condition
40
1 Core Shell structure
41
- Mix of NP during the growth of nanocrystals
- Sol-gel process
Core Shell synthesis
2 Dumbbell structures
42
- Mix of NP during the growth of nanocrystals
- Thermal rearrangement
- self-assemble at a liquid-liquid interface
- Functional sol-gel matrix
3 Multicomponent Hybrid NPs
Self-assembly processes provide an approach to
fabricating multicomponent hybrid NPs with
integrated multifunctionality
43
- Surfactant polymers
- Sol-gel process
44
Poly(lactico-co-glycolicacid)
(PLGA)
Poly(L-lysine)-PEG-folate
(PLL-PEG-FOL)
By mechanical
stirring at RT
in CH3Cl
45
46
W Lu SR Arumugam D Senapati ACSNANO 2010 4(3) 1739ndash1749
GNP GNP
GNP
RNA Aptamer
Anti-HER2C-reb-2 antibody
GNPCTAB
47
Magnetic nanoparticle surface modification Glucose oxidasse
(GOx)
PEGGPS
APSMNP
48Z Lin et al Analytica Chimica Acta 818 (2014) 54ndash60
49
P Sun H Zhang C Liu Langmuir 2010 26(2) 1278ndash1284 50
Immuno-labeling using Fe3O4CdTe nanocomposites which are formed by
linking multiple TGA-stabilized CdTe QDs with the thiol-functionalized silica
coated iron oxide nanoparticles
23-dimercaptosuccinic
acids (DMSA)Dopamine
Mercaptoundecanoic acid
(MUA)
HS COOH
4 Small multifunctional molecules
Glutaraldehyde 51
C J Xu K M Xu H W Gu R K Zheng H Liu X X Zhang Z H Guo B Xu J Am Chem Soc 2004 126 9938
Dopamine
Conditions (a) NaOH tert-butyl dicarbonate dioxane H2O 24 hrs (b) BnBr K2CO3 DMF
RT 24 h (c) 10 CF3COOH CH2Cl2 rt 5 h (d) succinic anhydride pyridine RTt 3 h (e)
NHS DCC DMAP CHCl3 RTt 3 h
NTA
52
3-mercapto trimethoxysilane
(MPS)
3-amino trimethoxysilane
(APS)
5 Silane multifunctional monomers
Glycidoxypropyltrimethoxy-silane
(GPS)
53
54
Multifunctional magnetic nanoparticles
application
Bioseparation
Biodetection(Real time monitoring)
Targeted drug delivery
Magnetic fluid Hyperthermia therapy
55
1 Specific Bio-Separation
bull Magnetic properties
bull Targeting agent
Cell organism molecule
56
His-tagged protein purification as
the first step towards recombinant
protein purification
58
Lysing Binding
Nickel
affinity
nanoparicle
His tag
fusion protein
Washing Elusion
Digestion
Binding
Nickel
affinity
nanoparticle
Washing Target
Recombinant
Protein
1- Preparation of Fe3O4SiO2Layered Double Hydroxide CoreminusShell
Microspheres for Magnetic Separation of Proteins J Am ChemSoc (2012)
18 134(2) 1071-7
Ni2+
2- Magnetic Nanocomposite Spheres Decorated with NiO Nanoparticles for a
Magnetically Recyclable Protein Separation System J Kim Y Piao N Lee Y
Park I-H Lee J-H Lee Adv Mater (2010) 22 57ndash60
His-tagged protein purification
Purification of His6-lipase using Fe3O4AundashLAndashIDAndashCu2+ NPs with various
amounts of NPs (lane 1 01 mg lane 2 02 mg lane 3
63
Synthetic procedure for the coreshell structure spheres
64
glutaraldehyde
(GLH)
Reference[1] H W Gu Z M Yang J H Gao C K Chang B Xu J Am Chem Soc 2005 127 34
[2] A Hutten D Sudfeld I Ennen G Reiss K Wojczykowski P Jutzi J Magn Magn Mater 2005
293 93
[3] G S Chaubey C Barcena N Poudyal C B Rong J M Gao S Sun J P Liu J Am Chem
Soc 2007 129 7214
[4] C W Kim Y H Kim H G Cha D K Lee Y S Kang J NanosciNanotechnol 2006 6 3417
[5] Y W Jun J S Choi J Cheon Angew Chem Int Ed 2006 45 3414
[6] X Michalet F F Pinaud L A Bentolila J M Tsay S Doose J J Li G Sundaresan A M Wu
S S Gambhir S Weiss Science 2005 307 538
[7] M Hu J Y Chen Z Y Li L Au G V Hartland X D Li M Marquez Y N Xia Chem Soc
Rev 2006 35 1084
[8] M Vallet-Regi F Balas D Arcos Angew Chem Int Ed 2007 46 7548
[9] S T Selvan P K Patra C Y Ang J Y Ying Angew Chem Int Ed 2007 46 2448
[10] I S Lee N Lee J Park B H Kim Y W Yi T Kim T K Kim I H Lee S R Paik T
Hyeon J Am Chem Soc 2006 128 10658
[11] Y S Lin S H Wu Y Hung Y H Chou C Chang M L Lin C P Tsai C Y Mou Chem
Mater 2006 18 5170
[12] B H Wu H Zhang C Chen S C Lin N F Zheng Nano Res 2009 2 975
[13] J Kim J E Lee J Lee J H Yu B C Kim K An Y Hwang C Shin J G Park J Kim T
Hyeon J Am Chem Soc 2006 128 688
[14] S Santra H S Yang P H Holloway J T Stanley R A Mericle J Am Chem Soc 2005 127
1656
[15] D K Yi S T Selvan S S Lee G C Papaefthymiou D Kundaliya J Y Ying J Am Chem
Soc 2005 127 4990 65
[16] J H Gao B Zhang Y Gao Y Pan X X Zhang B Xu J Am Chem Soc 2007 129 11928 [17] J H Gao W Zhang P B Huang B Zhang X X Zhang B Xu J Am Chem Soc 2008 130 3710 [18] J H Gao G L Liang B Zhang Y Kuang X X Zhang B Xu J Am Chem Soc 2007 129 1428 [19] J H Gao G L Liang J S Cheung Y Pan Y Kuang F Zhao B Zhang X X Zhang E X Wu B Xu J Am Chem Soc 2008 130 11828 [20] A R Kortan R Hull R L Opila M G Bawendi M L Steigerwald P J Carroll L E Brus JAm Chem Soc 1990 112 1327 [21] X G Peng M C Schlamp A V Kadavanich A P Alivisatos J Am Chem Soc 1997 119 7019 [22] S Kim B Fisher H J Eisler M Bawendi J Am Chem Soc 2003 125 11466[23] H Zeng J Li Z L Wang J P Liu S Sun Nano Lett 2004 4 187 [24] H Zeng S Sun J Li Z L Wang J P Liu Appl Phys Lett 2004 85 792 [25] Z Xu Y Hou S Sun J Am Chem Soc 2007 129 8698 [26] Y H Lin N Kim J Q Wang C J Zhong J Phys Chem B 2005 109 21593 [27] C Wang H Daimon S Sun Nano Lett 2009 9 1493 [28] K W Kwon M Shim J Am Chem Soc 2005 127 10269 [29] H Yu M Chen P M Rice S X Wang R L White S Sun Nano Lett 2005 5 379 [30] J S Choi Y W Jun S I Yeon H C Kim J S Shin J Cheon J Am Chem Soc 2006 128 15982 [31] S H Choi H B Na Y I Park K An S G Kwon Y Jang M Park J Moon J S Son I CSong W K Moon T Hyeon J Am Chem Soc 2008 130 15573 [32] H W Gu R K Zheng X X Zhang B Xu J Am Chem Soc 2004 126 5664
66
32
apto ldquoto fitrdquo
mer ldquosmallest unit of repeating structurerdquo
What is an Aptamer
Oligonucleotide or Peptide
molecules that bind to a specific
target molecule
33
Types of Aptamer
- ssDNA
- dsDNA
- RNA
- Peptide
34
SELEX(Systematic Evolution of Ligands by Exponential Enrichment)
Selection of ligand sequences that bind to a target
partitioning of aptamers from
non-aptamers via affinity methods
amplification of bound aptamers
35
Targets of Aptamers
- Small Molecules
- Proteins
- Nucleic Acids
- Cells
- Tissues
- Organisms
lysozym Thrombin HIV TAR Hemin Interferon γ Vascular
endotherial growth factor (VEGF) Prostate specific antigen(PSA)
Dopamine hellip 36
37
MFMNP Design
Core Shell
Dumbbell Structure
Multicomponent Hybrid NP
38
39
Polymer Shell
Core Shell
Metal alloy
(FePt)
Magnetic nanolayer
(Fe3O4)
Thermal decomposition
of Fe(acac)3 on FePt
Magnetic NP
(Fe3O4)
Metal alloy
(Au Ag)
(Fe3O4) in Au3+ solution
at reduction condition
40
1 Core Shell structure
41
- Mix of NP during the growth of nanocrystals
- Sol-gel process
Core Shell synthesis
2 Dumbbell structures
42
- Mix of NP during the growth of nanocrystals
- Thermal rearrangement
- self-assemble at a liquid-liquid interface
- Functional sol-gel matrix
3 Multicomponent Hybrid NPs
Self-assembly processes provide an approach to
fabricating multicomponent hybrid NPs with
integrated multifunctionality
43
- Surfactant polymers
- Sol-gel process
44
Poly(lactico-co-glycolicacid)
(PLGA)
Poly(L-lysine)-PEG-folate
(PLL-PEG-FOL)
By mechanical
stirring at RT
in CH3Cl
45
46
W Lu SR Arumugam D Senapati ACSNANO 2010 4(3) 1739ndash1749
GNP GNP
GNP
RNA Aptamer
Anti-HER2C-reb-2 antibody
GNPCTAB
47
Magnetic nanoparticle surface modification Glucose oxidasse
(GOx)
PEGGPS
APSMNP
48Z Lin et al Analytica Chimica Acta 818 (2014) 54ndash60
49
P Sun H Zhang C Liu Langmuir 2010 26(2) 1278ndash1284 50
Immuno-labeling using Fe3O4CdTe nanocomposites which are formed by
linking multiple TGA-stabilized CdTe QDs with the thiol-functionalized silica
coated iron oxide nanoparticles
23-dimercaptosuccinic
acids (DMSA)Dopamine
Mercaptoundecanoic acid
(MUA)
HS COOH
4 Small multifunctional molecules
Glutaraldehyde 51
C J Xu K M Xu H W Gu R K Zheng H Liu X X Zhang Z H Guo B Xu J Am Chem Soc 2004 126 9938
Dopamine
Conditions (a) NaOH tert-butyl dicarbonate dioxane H2O 24 hrs (b) BnBr K2CO3 DMF
RT 24 h (c) 10 CF3COOH CH2Cl2 rt 5 h (d) succinic anhydride pyridine RTt 3 h (e)
NHS DCC DMAP CHCl3 RTt 3 h
NTA
52
3-mercapto trimethoxysilane
(MPS)
3-amino trimethoxysilane
(APS)
5 Silane multifunctional monomers
Glycidoxypropyltrimethoxy-silane
(GPS)
53
54
Multifunctional magnetic nanoparticles
application
Bioseparation
Biodetection(Real time monitoring)
Targeted drug delivery
Magnetic fluid Hyperthermia therapy
55
1 Specific Bio-Separation
bull Magnetic properties
bull Targeting agent
Cell organism molecule
56
His-tagged protein purification as
the first step towards recombinant
protein purification
58
Lysing Binding
Nickel
affinity
nanoparicle
His tag
fusion protein
Washing Elusion
Digestion
Binding
Nickel
affinity
nanoparticle
Washing Target
Recombinant
Protein
1- Preparation of Fe3O4SiO2Layered Double Hydroxide CoreminusShell
Microspheres for Magnetic Separation of Proteins J Am ChemSoc (2012)
18 134(2) 1071-7
Ni2+
2- Magnetic Nanocomposite Spheres Decorated with NiO Nanoparticles for a
Magnetically Recyclable Protein Separation System J Kim Y Piao N Lee Y
Park I-H Lee J-H Lee Adv Mater (2010) 22 57ndash60
His-tagged protein purification
Purification of His6-lipase using Fe3O4AundashLAndashIDAndashCu2+ NPs with various
amounts of NPs (lane 1 01 mg lane 2 02 mg lane 3
63
Synthetic procedure for the coreshell structure spheres
64
glutaraldehyde
(GLH)
Reference[1] H W Gu Z M Yang J H Gao C K Chang B Xu J Am Chem Soc 2005 127 34
[2] A Hutten D Sudfeld I Ennen G Reiss K Wojczykowski P Jutzi J Magn Magn Mater 2005
293 93
[3] G S Chaubey C Barcena N Poudyal C B Rong J M Gao S Sun J P Liu J Am Chem
Soc 2007 129 7214
[4] C W Kim Y H Kim H G Cha D K Lee Y S Kang J NanosciNanotechnol 2006 6 3417
[5] Y W Jun J S Choi J Cheon Angew Chem Int Ed 2006 45 3414
[6] X Michalet F F Pinaud L A Bentolila J M Tsay S Doose J J Li G Sundaresan A M Wu
S S Gambhir S Weiss Science 2005 307 538
[7] M Hu J Y Chen Z Y Li L Au G V Hartland X D Li M Marquez Y N Xia Chem Soc
Rev 2006 35 1084
[8] M Vallet-Regi F Balas D Arcos Angew Chem Int Ed 2007 46 7548
[9] S T Selvan P K Patra C Y Ang J Y Ying Angew Chem Int Ed 2007 46 2448
[10] I S Lee N Lee J Park B H Kim Y W Yi T Kim T K Kim I H Lee S R Paik T
Hyeon J Am Chem Soc 2006 128 10658
[11] Y S Lin S H Wu Y Hung Y H Chou C Chang M L Lin C P Tsai C Y Mou Chem
Mater 2006 18 5170
[12] B H Wu H Zhang C Chen S C Lin N F Zheng Nano Res 2009 2 975
[13] J Kim J E Lee J Lee J H Yu B C Kim K An Y Hwang C Shin J G Park J Kim T
Hyeon J Am Chem Soc 2006 128 688
[14] S Santra H S Yang P H Holloway J T Stanley R A Mericle J Am Chem Soc 2005 127
1656
[15] D K Yi S T Selvan S S Lee G C Papaefthymiou D Kundaliya J Y Ying J Am Chem
Soc 2005 127 4990 65
[16] J H Gao B Zhang Y Gao Y Pan X X Zhang B Xu J Am Chem Soc 2007 129 11928 [17] J H Gao W Zhang P B Huang B Zhang X X Zhang B Xu J Am Chem Soc 2008 130 3710 [18] J H Gao G L Liang B Zhang Y Kuang X X Zhang B Xu J Am Chem Soc 2007 129 1428 [19] J H Gao G L Liang J S Cheung Y Pan Y Kuang F Zhao B Zhang X X Zhang E X Wu B Xu J Am Chem Soc 2008 130 11828 [20] A R Kortan R Hull R L Opila M G Bawendi M L Steigerwald P J Carroll L E Brus JAm Chem Soc 1990 112 1327 [21] X G Peng M C Schlamp A V Kadavanich A P Alivisatos J Am Chem Soc 1997 119 7019 [22] S Kim B Fisher H J Eisler M Bawendi J Am Chem Soc 2003 125 11466[23] H Zeng J Li Z L Wang J P Liu S Sun Nano Lett 2004 4 187 [24] H Zeng S Sun J Li Z L Wang J P Liu Appl Phys Lett 2004 85 792 [25] Z Xu Y Hou S Sun J Am Chem Soc 2007 129 8698 [26] Y H Lin N Kim J Q Wang C J Zhong J Phys Chem B 2005 109 21593 [27] C Wang H Daimon S Sun Nano Lett 2009 9 1493 [28] K W Kwon M Shim J Am Chem Soc 2005 127 10269 [29] H Yu M Chen P M Rice S X Wang R L White S Sun Nano Lett 2005 5 379 [30] J S Choi Y W Jun S I Yeon H C Kim J S Shin J Cheon J Am Chem Soc 2006 128 15982 [31] S H Choi H B Na Y I Park K An S G Kwon Y Jang M Park J Moon J S Son I CSong W K Moon T Hyeon J Am Chem Soc 2008 130 15573 [32] H W Gu R K Zheng X X Zhang B Xu J Am Chem Soc 2004 126 5664
66
apto ldquoto fitrdquo
mer ldquosmallest unit of repeating structurerdquo
What is an Aptamer
Oligonucleotide or Peptide
molecules that bind to a specific
target molecule
33
Types of Aptamer
- ssDNA
- dsDNA
- RNA
- Peptide
34
SELEX(Systematic Evolution of Ligands by Exponential Enrichment)
Selection of ligand sequences that bind to a target
partitioning of aptamers from
non-aptamers via affinity methods
amplification of bound aptamers
35
Targets of Aptamers
- Small Molecules
- Proteins
- Nucleic Acids
- Cells
- Tissues
- Organisms
lysozym Thrombin HIV TAR Hemin Interferon γ Vascular
endotherial growth factor (VEGF) Prostate specific antigen(PSA)
Dopamine hellip 36
37
MFMNP Design
Core Shell
Dumbbell Structure
Multicomponent Hybrid NP
38
39
Polymer Shell
Core Shell
Metal alloy
(FePt)
Magnetic nanolayer
(Fe3O4)
Thermal decomposition
of Fe(acac)3 on FePt
Magnetic NP
(Fe3O4)
Metal alloy
(Au Ag)
(Fe3O4) in Au3+ solution
at reduction condition
40
1 Core Shell structure
41
- Mix of NP during the growth of nanocrystals
- Sol-gel process
Core Shell synthesis
2 Dumbbell structures
42
- Mix of NP during the growth of nanocrystals
- Thermal rearrangement
- self-assemble at a liquid-liquid interface
- Functional sol-gel matrix
3 Multicomponent Hybrid NPs
Self-assembly processes provide an approach to
fabricating multicomponent hybrid NPs with
integrated multifunctionality
43
- Surfactant polymers
- Sol-gel process
44
Poly(lactico-co-glycolicacid)
(PLGA)
Poly(L-lysine)-PEG-folate
(PLL-PEG-FOL)
By mechanical
stirring at RT
in CH3Cl
45
46
W Lu SR Arumugam D Senapati ACSNANO 2010 4(3) 1739ndash1749
GNP GNP
GNP
RNA Aptamer
Anti-HER2C-reb-2 antibody
GNPCTAB
47
Magnetic nanoparticle surface modification Glucose oxidasse
(GOx)
PEGGPS
APSMNP
48Z Lin et al Analytica Chimica Acta 818 (2014) 54ndash60
49
P Sun H Zhang C Liu Langmuir 2010 26(2) 1278ndash1284 50
Immuno-labeling using Fe3O4CdTe nanocomposites which are formed by
linking multiple TGA-stabilized CdTe QDs with the thiol-functionalized silica
coated iron oxide nanoparticles
23-dimercaptosuccinic
acids (DMSA)Dopamine
Mercaptoundecanoic acid
(MUA)
HS COOH
4 Small multifunctional molecules
Glutaraldehyde 51
C J Xu K M Xu H W Gu R K Zheng H Liu X X Zhang Z H Guo B Xu J Am Chem Soc 2004 126 9938
Dopamine
Conditions (a) NaOH tert-butyl dicarbonate dioxane H2O 24 hrs (b) BnBr K2CO3 DMF
RT 24 h (c) 10 CF3COOH CH2Cl2 rt 5 h (d) succinic anhydride pyridine RTt 3 h (e)
NHS DCC DMAP CHCl3 RTt 3 h
NTA
52
3-mercapto trimethoxysilane
(MPS)
3-amino trimethoxysilane
(APS)
5 Silane multifunctional monomers
Glycidoxypropyltrimethoxy-silane
(GPS)
53
54
Multifunctional magnetic nanoparticles
application
Bioseparation
Biodetection(Real time monitoring)
Targeted drug delivery
Magnetic fluid Hyperthermia therapy
55
1 Specific Bio-Separation
bull Magnetic properties
bull Targeting agent
Cell organism molecule
56
His-tagged protein purification as
the first step towards recombinant
protein purification
58
Lysing Binding
Nickel
affinity
nanoparicle
His tag
fusion protein
Washing Elusion
Digestion
Binding
Nickel
affinity
nanoparticle
Washing Target
Recombinant
Protein
1- Preparation of Fe3O4SiO2Layered Double Hydroxide CoreminusShell
Microspheres for Magnetic Separation of Proteins J Am ChemSoc (2012)
18 134(2) 1071-7
Ni2+
2- Magnetic Nanocomposite Spheres Decorated with NiO Nanoparticles for a
Magnetically Recyclable Protein Separation System J Kim Y Piao N Lee Y
Park I-H Lee J-H Lee Adv Mater (2010) 22 57ndash60
His-tagged protein purification
Purification of His6-lipase using Fe3O4AundashLAndashIDAndashCu2+ NPs with various
amounts of NPs (lane 1 01 mg lane 2 02 mg lane 3
63
Synthetic procedure for the coreshell structure spheres
64
glutaraldehyde
(GLH)
Reference[1] H W Gu Z M Yang J H Gao C K Chang B Xu J Am Chem Soc 2005 127 34
[2] A Hutten D Sudfeld I Ennen G Reiss K Wojczykowski P Jutzi J Magn Magn Mater 2005
293 93
[3] G S Chaubey C Barcena N Poudyal C B Rong J M Gao S Sun J P Liu J Am Chem
Soc 2007 129 7214
[4] C W Kim Y H Kim H G Cha D K Lee Y S Kang J NanosciNanotechnol 2006 6 3417
[5] Y W Jun J S Choi J Cheon Angew Chem Int Ed 2006 45 3414
[6] X Michalet F F Pinaud L A Bentolila J M Tsay S Doose J J Li G Sundaresan A M Wu
S S Gambhir S Weiss Science 2005 307 538
[7] M Hu J Y Chen Z Y Li L Au G V Hartland X D Li M Marquez Y N Xia Chem Soc
Rev 2006 35 1084
[8] M Vallet-Regi F Balas D Arcos Angew Chem Int Ed 2007 46 7548
[9] S T Selvan P K Patra C Y Ang J Y Ying Angew Chem Int Ed 2007 46 2448
[10] I S Lee N Lee J Park B H Kim Y W Yi T Kim T K Kim I H Lee S R Paik T
Hyeon J Am Chem Soc 2006 128 10658
[11] Y S Lin S H Wu Y Hung Y H Chou C Chang M L Lin C P Tsai C Y Mou Chem
Mater 2006 18 5170
[12] B H Wu H Zhang C Chen S C Lin N F Zheng Nano Res 2009 2 975
[13] J Kim J E Lee J Lee J H Yu B C Kim K An Y Hwang C Shin J G Park J Kim T
Hyeon J Am Chem Soc 2006 128 688
[14] S Santra H S Yang P H Holloway J T Stanley R A Mericle J Am Chem Soc 2005 127
1656
[15] D K Yi S T Selvan S S Lee G C Papaefthymiou D Kundaliya J Y Ying J Am Chem
Soc 2005 127 4990 65
[16] J H Gao B Zhang Y Gao Y Pan X X Zhang B Xu J Am Chem Soc 2007 129 11928 [17] J H Gao W Zhang P B Huang B Zhang X X Zhang B Xu J Am Chem Soc 2008 130 3710 [18] J H Gao G L Liang B Zhang Y Kuang X X Zhang B Xu J Am Chem Soc 2007 129 1428 [19] J H Gao G L Liang J S Cheung Y Pan Y Kuang F Zhao B Zhang X X Zhang E X Wu B Xu J Am Chem Soc 2008 130 11828 [20] A R Kortan R Hull R L Opila M G Bawendi M L Steigerwald P J Carroll L E Brus JAm Chem Soc 1990 112 1327 [21] X G Peng M C Schlamp A V Kadavanich A P Alivisatos J Am Chem Soc 1997 119 7019 [22] S Kim B Fisher H J Eisler M Bawendi J Am Chem Soc 2003 125 11466[23] H Zeng J Li Z L Wang J P Liu S Sun Nano Lett 2004 4 187 [24] H Zeng S Sun J Li Z L Wang J P Liu Appl Phys Lett 2004 85 792 [25] Z Xu Y Hou S Sun J Am Chem Soc 2007 129 8698 [26] Y H Lin N Kim J Q Wang C J Zhong J Phys Chem B 2005 109 21593 [27] C Wang H Daimon S Sun Nano Lett 2009 9 1493 [28] K W Kwon M Shim J Am Chem Soc 2005 127 10269 [29] H Yu M Chen P M Rice S X Wang R L White S Sun Nano Lett 2005 5 379 [30] J S Choi Y W Jun S I Yeon H C Kim J S Shin J Cheon J Am Chem Soc 2006 128 15982 [31] S H Choi H B Na Y I Park K An S G Kwon Y Jang M Park J Moon J S Son I CSong W K Moon T Hyeon J Am Chem Soc 2008 130 15573 [32] H W Gu R K Zheng X X Zhang B Xu J Am Chem Soc 2004 126 5664
66
Types of Aptamer
- ssDNA
- dsDNA
- RNA
- Peptide
34
SELEX(Systematic Evolution of Ligands by Exponential Enrichment)
Selection of ligand sequences that bind to a target
partitioning of aptamers from
non-aptamers via affinity methods
amplification of bound aptamers
35
Targets of Aptamers
- Small Molecules
- Proteins
- Nucleic Acids
- Cells
- Tissues
- Organisms
lysozym Thrombin HIV TAR Hemin Interferon γ Vascular
endotherial growth factor (VEGF) Prostate specific antigen(PSA)
Dopamine hellip 36
37
MFMNP Design
Core Shell
Dumbbell Structure
Multicomponent Hybrid NP
38
39
Polymer Shell
Core Shell
Metal alloy
(FePt)
Magnetic nanolayer
(Fe3O4)
Thermal decomposition
of Fe(acac)3 on FePt
Magnetic NP
(Fe3O4)
Metal alloy
(Au Ag)
(Fe3O4) in Au3+ solution
at reduction condition
40
1 Core Shell structure
41
- Mix of NP during the growth of nanocrystals
- Sol-gel process
Core Shell synthesis
2 Dumbbell structures
42
- Mix of NP during the growth of nanocrystals
- Thermal rearrangement
- self-assemble at a liquid-liquid interface
- Functional sol-gel matrix
3 Multicomponent Hybrid NPs
Self-assembly processes provide an approach to
fabricating multicomponent hybrid NPs with
integrated multifunctionality
43
- Surfactant polymers
- Sol-gel process
44
Poly(lactico-co-glycolicacid)
(PLGA)
Poly(L-lysine)-PEG-folate
(PLL-PEG-FOL)
By mechanical
stirring at RT
in CH3Cl
45
46
W Lu SR Arumugam D Senapati ACSNANO 2010 4(3) 1739ndash1749
GNP GNP
GNP
RNA Aptamer
Anti-HER2C-reb-2 antibody
GNPCTAB
47
Magnetic nanoparticle surface modification Glucose oxidasse
(GOx)
PEGGPS
APSMNP
48Z Lin et al Analytica Chimica Acta 818 (2014) 54ndash60
49
P Sun H Zhang C Liu Langmuir 2010 26(2) 1278ndash1284 50
Immuno-labeling using Fe3O4CdTe nanocomposites which are formed by
linking multiple TGA-stabilized CdTe QDs with the thiol-functionalized silica
coated iron oxide nanoparticles
23-dimercaptosuccinic
acids (DMSA)Dopamine
Mercaptoundecanoic acid
(MUA)
HS COOH
4 Small multifunctional molecules
Glutaraldehyde 51
C J Xu K M Xu H W Gu R K Zheng H Liu X X Zhang Z H Guo B Xu J Am Chem Soc 2004 126 9938
Dopamine
Conditions (a) NaOH tert-butyl dicarbonate dioxane H2O 24 hrs (b) BnBr K2CO3 DMF
RT 24 h (c) 10 CF3COOH CH2Cl2 rt 5 h (d) succinic anhydride pyridine RTt 3 h (e)
NHS DCC DMAP CHCl3 RTt 3 h
NTA
52
3-mercapto trimethoxysilane
(MPS)
3-amino trimethoxysilane
(APS)
5 Silane multifunctional monomers
Glycidoxypropyltrimethoxy-silane
(GPS)
53
54
Multifunctional magnetic nanoparticles
application
Bioseparation
Biodetection(Real time monitoring)
Targeted drug delivery
Magnetic fluid Hyperthermia therapy
55
1 Specific Bio-Separation
bull Magnetic properties
bull Targeting agent
Cell organism molecule
56
His-tagged protein purification as
the first step towards recombinant
protein purification
58
Lysing Binding
Nickel
affinity
nanoparicle
His tag
fusion protein
Washing Elusion
Digestion
Binding
Nickel
affinity
nanoparticle
Washing Target
Recombinant
Protein
1- Preparation of Fe3O4SiO2Layered Double Hydroxide CoreminusShell
Microspheres for Magnetic Separation of Proteins J Am ChemSoc (2012)
18 134(2) 1071-7
Ni2+
2- Magnetic Nanocomposite Spheres Decorated with NiO Nanoparticles for a
Magnetically Recyclable Protein Separation System J Kim Y Piao N Lee Y
Park I-H Lee J-H Lee Adv Mater (2010) 22 57ndash60
His-tagged protein purification
Purification of His6-lipase using Fe3O4AundashLAndashIDAndashCu2+ NPs with various
amounts of NPs (lane 1 01 mg lane 2 02 mg lane 3
63
Synthetic procedure for the coreshell structure spheres
64
glutaraldehyde
(GLH)
Reference[1] H W Gu Z M Yang J H Gao C K Chang B Xu J Am Chem Soc 2005 127 34
[2] A Hutten D Sudfeld I Ennen G Reiss K Wojczykowski P Jutzi J Magn Magn Mater 2005
293 93
[3] G S Chaubey C Barcena N Poudyal C B Rong J M Gao S Sun J P Liu J Am Chem
Soc 2007 129 7214
[4] C W Kim Y H Kim H G Cha D K Lee Y S Kang J NanosciNanotechnol 2006 6 3417
[5] Y W Jun J S Choi J Cheon Angew Chem Int Ed 2006 45 3414
[6] X Michalet F F Pinaud L A Bentolila J M Tsay S Doose J J Li G Sundaresan A M Wu
S S Gambhir S Weiss Science 2005 307 538
[7] M Hu J Y Chen Z Y Li L Au G V Hartland X D Li M Marquez Y N Xia Chem Soc
Rev 2006 35 1084
[8] M Vallet-Regi F Balas D Arcos Angew Chem Int Ed 2007 46 7548
[9] S T Selvan P K Patra C Y Ang J Y Ying Angew Chem Int Ed 2007 46 2448
[10] I S Lee N Lee J Park B H Kim Y W Yi T Kim T K Kim I H Lee S R Paik T
Hyeon J Am Chem Soc 2006 128 10658
[11] Y S Lin S H Wu Y Hung Y H Chou C Chang M L Lin C P Tsai C Y Mou Chem
Mater 2006 18 5170
[12] B H Wu H Zhang C Chen S C Lin N F Zheng Nano Res 2009 2 975
[13] J Kim J E Lee J Lee J H Yu B C Kim K An Y Hwang C Shin J G Park J Kim T
Hyeon J Am Chem Soc 2006 128 688
[14] S Santra H S Yang P H Holloway J T Stanley R A Mericle J Am Chem Soc 2005 127
1656
[15] D K Yi S T Selvan S S Lee G C Papaefthymiou D Kundaliya J Y Ying J Am Chem
Soc 2005 127 4990 65
[16] J H Gao B Zhang Y Gao Y Pan X X Zhang B Xu J Am Chem Soc 2007 129 11928 [17] J H Gao W Zhang P B Huang B Zhang X X Zhang B Xu J Am Chem Soc 2008 130 3710 [18] J H Gao G L Liang B Zhang Y Kuang X X Zhang B Xu J Am Chem Soc 2007 129 1428 [19] J H Gao G L Liang J S Cheung Y Pan Y Kuang F Zhao B Zhang X X Zhang E X Wu B Xu J Am Chem Soc 2008 130 11828 [20] A R Kortan R Hull R L Opila M G Bawendi M L Steigerwald P J Carroll L E Brus JAm Chem Soc 1990 112 1327 [21] X G Peng M C Schlamp A V Kadavanich A P Alivisatos J Am Chem Soc 1997 119 7019 [22] S Kim B Fisher H J Eisler M Bawendi J Am Chem Soc 2003 125 11466[23] H Zeng J Li Z L Wang J P Liu S Sun Nano Lett 2004 4 187 [24] H Zeng S Sun J Li Z L Wang J P Liu Appl Phys Lett 2004 85 792 [25] Z Xu Y Hou S Sun J Am Chem Soc 2007 129 8698 [26] Y H Lin N Kim J Q Wang C J Zhong J Phys Chem B 2005 109 21593 [27] C Wang H Daimon S Sun Nano Lett 2009 9 1493 [28] K W Kwon M Shim J Am Chem Soc 2005 127 10269 [29] H Yu M Chen P M Rice S X Wang R L White S Sun Nano Lett 2005 5 379 [30] J S Choi Y W Jun S I Yeon H C Kim J S Shin J Cheon J Am Chem Soc 2006 128 15982 [31] S H Choi H B Na Y I Park K An S G Kwon Y Jang M Park J Moon J S Son I CSong W K Moon T Hyeon J Am Chem Soc 2008 130 15573 [32] H W Gu R K Zheng X X Zhang B Xu J Am Chem Soc 2004 126 5664
66
SELEX(Systematic Evolution of Ligands by Exponential Enrichment)
Selection of ligand sequences that bind to a target
partitioning of aptamers from
non-aptamers via affinity methods
amplification of bound aptamers
35
Targets of Aptamers
- Small Molecules
- Proteins
- Nucleic Acids
- Cells
- Tissues
- Organisms
lysozym Thrombin HIV TAR Hemin Interferon γ Vascular
endotherial growth factor (VEGF) Prostate specific antigen(PSA)
Dopamine hellip 36
37
MFMNP Design
Core Shell
Dumbbell Structure
Multicomponent Hybrid NP
38
39
Polymer Shell
Core Shell
Metal alloy
(FePt)
Magnetic nanolayer
(Fe3O4)
Thermal decomposition
of Fe(acac)3 on FePt
Magnetic NP
(Fe3O4)
Metal alloy
(Au Ag)
(Fe3O4) in Au3+ solution
at reduction condition
40
1 Core Shell structure
41
- Mix of NP during the growth of nanocrystals
- Sol-gel process
Core Shell synthesis
2 Dumbbell structures
42
- Mix of NP during the growth of nanocrystals
- Thermal rearrangement
- self-assemble at a liquid-liquid interface
- Functional sol-gel matrix
3 Multicomponent Hybrid NPs
Self-assembly processes provide an approach to
fabricating multicomponent hybrid NPs with
integrated multifunctionality
43
- Surfactant polymers
- Sol-gel process
44
Poly(lactico-co-glycolicacid)
(PLGA)
Poly(L-lysine)-PEG-folate
(PLL-PEG-FOL)
By mechanical
stirring at RT
in CH3Cl
45
46
W Lu SR Arumugam D Senapati ACSNANO 2010 4(3) 1739ndash1749
GNP GNP
GNP
RNA Aptamer
Anti-HER2C-reb-2 antibody
GNPCTAB
47
Magnetic nanoparticle surface modification Glucose oxidasse
(GOx)
PEGGPS
APSMNP
48Z Lin et al Analytica Chimica Acta 818 (2014) 54ndash60
49
P Sun H Zhang C Liu Langmuir 2010 26(2) 1278ndash1284 50
Immuno-labeling using Fe3O4CdTe nanocomposites which are formed by
linking multiple TGA-stabilized CdTe QDs with the thiol-functionalized silica
coated iron oxide nanoparticles
23-dimercaptosuccinic
acids (DMSA)Dopamine
Mercaptoundecanoic acid
(MUA)
HS COOH
4 Small multifunctional molecules
Glutaraldehyde 51
C J Xu K M Xu H W Gu R K Zheng H Liu X X Zhang Z H Guo B Xu J Am Chem Soc 2004 126 9938
Dopamine
Conditions (a) NaOH tert-butyl dicarbonate dioxane H2O 24 hrs (b) BnBr K2CO3 DMF
RT 24 h (c) 10 CF3COOH CH2Cl2 rt 5 h (d) succinic anhydride pyridine RTt 3 h (e)
NHS DCC DMAP CHCl3 RTt 3 h
NTA
52
3-mercapto trimethoxysilane
(MPS)
3-amino trimethoxysilane
(APS)
5 Silane multifunctional monomers
Glycidoxypropyltrimethoxy-silane
(GPS)
53
54
Multifunctional magnetic nanoparticles
application
Bioseparation
Biodetection(Real time monitoring)
Targeted drug delivery
Magnetic fluid Hyperthermia therapy
55
1 Specific Bio-Separation
bull Magnetic properties
bull Targeting agent
Cell organism molecule
56
His-tagged protein purification as
the first step towards recombinant
protein purification
58
Lysing Binding
Nickel
affinity
nanoparicle
His tag
fusion protein
Washing Elusion
Digestion
Binding
Nickel
affinity
nanoparticle
Washing Target
Recombinant
Protein
1- Preparation of Fe3O4SiO2Layered Double Hydroxide CoreminusShell
Microspheres for Magnetic Separation of Proteins J Am ChemSoc (2012)
18 134(2) 1071-7
Ni2+
2- Magnetic Nanocomposite Spheres Decorated with NiO Nanoparticles for a
Magnetically Recyclable Protein Separation System J Kim Y Piao N Lee Y
Park I-H Lee J-H Lee Adv Mater (2010) 22 57ndash60
His-tagged protein purification
Purification of His6-lipase using Fe3O4AundashLAndashIDAndashCu2+ NPs with various
amounts of NPs (lane 1 01 mg lane 2 02 mg lane 3
63
Synthetic procedure for the coreshell structure spheres
64
glutaraldehyde
(GLH)
Reference[1] H W Gu Z M Yang J H Gao C K Chang B Xu J Am Chem Soc 2005 127 34
[2] A Hutten D Sudfeld I Ennen G Reiss K Wojczykowski P Jutzi J Magn Magn Mater 2005
293 93
[3] G S Chaubey C Barcena N Poudyal C B Rong J M Gao S Sun J P Liu J Am Chem
Soc 2007 129 7214
[4] C W Kim Y H Kim H G Cha D K Lee Y S Kang J NanosciNanotechnol 2006 6 3417
[5] Y W Jun J S Choi J Cheon Angew Chem Int Ed 2006 45 3414
[6] X Michalet F F Pinaud L A Bentolila J M Tsay S Doose J J Li G Sundaresan A M Wu
S S Gambhir S Weiss Science 2005 307 538
[7] M Hu J Y Chen Z Y Li L Au G V Hartland X D Li M Marquez Y N Xia Chem Soc
Rev 2006 35 1084
[8] M Vallet-Regi F Balas D Arcos Angew Chem Int Ed 2007 46 7548
[9] S T Selvan P K Patra C Y Ang J Y Ying Angew Chem Int Ed 2007 46 2448
[10] I S Lee N Lee J Park B H Kim Y W Yi T Kim T K Kim I H Lee S R Paik T
Hyeon J Am Chem Soc 2006 128 10658
[11] Y S Lin S H Wu Y Hung Y H Chou C Chang M L Lin C P Tsai C Y Mou Chem
Mater 2006 18 5170
[12] B H Wu H Zhang C Chen S C Lin N F Zheng Nano Res 2009 2 975
[13] J Kim J E Lee J Lee J H Yu B C Kim K An Y Hwang C Shin J G Park J Kim T
Hyeon J Am Chem Soc 2006 128 688
[14] S Santra H S Yang P H Holloway J T Stanley R A Mericle J Am Chem Soc 2005 127
1656
[15] D K Yi S T Selvan S S Lee G C Papaefthymiou D Kundaliya J Y Ying J Am Chem
Soc 2005 127 4990 65
[16] J H Gao B Zhang Y Gao Y Pan X X Zhang B Xu J Am Chem Soc 2007 129 11928 [17] J H Gao W Zhang P B Huang B Zhang X X Zhang B Xu J Am Chem Soc 2008 130 3710 [18] J H Gao G L Liang B Zhang Y Kuang X X Zhang B Xu J Am Chem Soc 2007 129 1428 [19] J H Gao G L Liang J S Cheung Y Pan Y Kuang F Zhao B Zhang X X Zhang E X Wu B Xu J Am Chem Soc 2008 130 11828 [20] A R Kortan R Hull R L Opila M G Bawendi M L Steigerwald P J Carroll L E Brus JAm Chem Soc 1990 112 1327 [21] X G Peng M C Schlamp A V Kadavanich A P Alivisatos J Am Chem Soc 1997 119 7019 [22] S Kim B Fisher H J Eisler M Bawendi J Am Chem Soc 2003 125 11466[23] H Zeng J Li Z L Wang J P Liu S Sun Nano Lett 2004 4 187 [24] H Zeng S Sun J Li Z L Wang J P Liu Appl Phys Lett 2004 85 792 [25] Z Xu Y Hou S Sun J Am Chem Soc 2007 129 8698 [26] Y H Lin N Kim J Q Wang C J Zhong J Phys Chem B 2005 109 21593 [27] C Wang H Daimon S Sun Nano Lett 2009 9 1493 [28] K W Kwon M Shim J Am Chem Soc 2005 127 10269 [29] H Yu M Chen P M Rice S X Wang R L White S Sun Nano Lett 2005 5 379 [30] J S Choi Y W Jun S I Yeon H C Kim J S Shin J Cheon J Am Chem Soc 2006 128 15982 [31] S H Choi H B Na Y I Park K An S G Kwon Y Jang M Park J Moon J S Son I CSong W K Moon T Hyeon J Am Chem Soc 2008 130 15573 [32] H W Gu R K Zheng X X Zhang B Xu J Am Chem Soc 2004 126 5664
66
Targets of Aptamers
- Small Molecules
- Proteins
- Nucleic Acids
- Cells
- Tissues
- Organisms
lysozym Thrombin HIV TAR Hemin Interferon γ Vascular
endotherial growth factor (VEGF) Prostate specific antigen(PSA)
Dopamine hellip 36
37
MFMNP Design
Core Shell
Dumbbell Structure
Multicomponent Hybrid NP
38
39
Polymer Shell
Core Shell
Metal alloy
(FePt)
Magnetic nanolayer
(Fe3O4)
Thermal decomposition
of Fe(acac)3 on FePt
Magnetic NP
(Fe3O4)
Metal alloy
(Au Ag)
(Fe3O4) in Au3+ solution
at reduction condition
40
1 Core Shell structure
41
- Mix of NP during the growth of nanocrystals
- Sol-gel process
Core Shell synthesis
2 Dumbbell structures
42
- Mix of NP during the growth of nanocrystals
- Thermal rearrangement
- self-assemble at a liquid-liquid interface
- Functional sol-gel matrix
3 Multicomponent Hybrid NPs
Self-assembly processes provide an approach to
fabricating multicomponent hybrid NPs with
integrated multifunctionality
43
- Surfactant polymers
- Sol-gel process
44
Poly(lactico-co-glycolicacid)
(PLGA)
Poly(L-lysine)-PEG-folate
(PLL-PEG-FOL)
By mechanical
stirring at RT
in CH3Cl
45
46
W Lu SR Arumugam D Senapati ACSNANO 2010 4(3) 1739ndash1749
GNP GNP
GNP
RNA Aptamer
Anti-HER2C-reb-2 antibody
GNPCTAB
47
Magnetic nanoparticle surface modification Glucose oxidasse
(GOx)
PEGGPS
APSMNP
48Z Lin et al Analytica Chimica Acta 818 (2014) 54ndash60
49
P Sun H Zhang C Liu Langmuir 2010 26(2) 1278ndash1284 50
Immuno-labeling using Fe3O4CdTe nanocomposites which are formed by
linking multiple TGA-stabilized CdTe QDs with the thiol-functionalized silica
coated iron oxide nanoparticles
23-dimercaptosuccinic
acids (DMSA)Dopamine
Mercaptoundecanoic acid
(MUA)
HS COOH
4 Small multifunctional molecules
Glutaraldehyde 51
C J Xu K M Xu H W Gu R K Zheng H Liu X X Zhang Z H Guo B Xu J Am Chem Soc 2004 126 9938
Dopamine
Conditions (a) NaOH tert-butyl dicarbonate dioxane H2O 24 hrs (b) BnBr K2CO3 DMF
RT 24 h (c) 10 CF3COOH CH2Cl2 rt 5 h (d) succinic anhydride pyridine RTt 3 h (e)
NHS DCC DMAP CHCl3 RTt 3 h
NTA
52
3-mercapto trimethoxysilane
(MPS)
3-amino trimethoxysilane
(APS)
5 Silane multifunctional monomers
Glycidoxypropyltrimethoxy-silane
(GPS)
53
54
Multifunctional magnetic nanoparticles
application
Bioseparation
Biodetection(Real time monitoring)
Targeted drug delivery
Magnetic fluid Hyperthermia therapy
55
1 Specific Bio-Separation
bull Magnetic properties
bull Targeting agent
Cell organism molecule
56
His-tagged protein purification as
the first step towards recombinant
protein purification
58
Lysing Binding
Nickel
affinity
nanoparicle
His tag
fusion protein
Washing Elusion
Digestion
Binding
Nickel
affinity
nanoparticle
Washing Target
Recombinant
Protein
1- Preparation of Fe3O4SiO2Layered Double Hydroxide CoreminusShell
Microspheres for Magnetic Separation of Proteins J Am ChemSoc (2012)
18 134(2) 1071-7
Ni2+
2- Magnetic Nanocomposite Spheres Decorated with NiO Nanoparticles for a
Magnetically Recyclable Protein Separation System J Kim Y Piao N Lee Y
Park I-H Lee J-H Lee Adv Mater (2010) 22 57ndash60
His-tagged protein purification
Purification of His6-lipase using Fe3O4AundashLAndashIDAndashCu2+ NPs with various
amounts of NPs (lane 1 01 mg lane 2 02 mg lane 3
63
Synthetic procedure for the coreshell structure spheres
64
glutaraldehyde
(GLH)
Reference[1] H W Gu Z M Yang J H Gao C K Chang B Xu J Am Chem Soc 2005 127 34
[2] A Hutten D Sudfeld I Ennen G Reiss K Wojczykowski P Jutzi J Magn Magn Mater 2005
293 93
[3] G S Chaubey C Barcena N Poudyal C B Rong J M Gao S Sun J P Liu J Am Chem
Soc 2007 129 7214
[4] C W Kim Y H Kim H G Cha D K Lee Y S Kang J NanosciNanotechnol 2006 6 3417
[5] Y W Jun J S Choi J Cheon Angew Chem Int Ed 2006 45 3414
[6] X Michalet F F Pinaud L A Bentolila J M Tsay S Doose J J Li G Sundaresan A M Wu
S S Gambhir S Weiss Science 2005 307 538
[7] M Hu J Y Chen Z Y Li L Au G V Hartland X D Li M Marquez Y N Xia Chem Soc
Rev 2006 35 1084
[8] M Vallet-Regi F Balas D Arcos Angew Chem Int Ed 2007 46 7548
[9] S T Selvan P K Patra C Y Ang J Y Ying Angew Chem Int Ed 2007 46 2448
[10] I S Lee N Lee J Park B H Kim Y W Yi T Kim T K Kim I H Lee S R Paik T
Hyeon J Am Chem Soc 2006 128 10658
[11] Y S Lin S H Wu Y Hung Y H Chou C Chang M L Lin C P Tsai C Y Mou Chem
Mater 2006 18 5170
[12] B H Wu H Zhang C Chen S C Lin N F Zheng Nano Res 2009 2 975
[13] J Kim J E Lee J Lee J H Yu B C Kim K An Y Hwang C Shin J G Park J Kim T
Hyeon J Am Chem Soc 2006 128 688
[14] S Santra H S Yang P H Holloway J T Stanley R A Mericle J Am Chem Soc 2005 127
1656
[15] D K Yi S T Selvan S S Lee G C Papaefthymiou D Kundaliya J Y Ying J Am Chem
Soc 2005 127 4990 65
[16] J H Gao B Zhang Y Gao Y Pan X X Zhang B Xu J Am Chem Soc 2007 129 11928 [17] J H Gao W Zhang P B Huang B Zhang X X Zhang B Xu J Am Chem Soc 2008 130 3710 [18] J H Gao G L Liang B Zhang Y Kuang X X Zhang B Xu J Am Chem Soc 2007 129 1428 [19] J H Gao G L Liang J S Cheung Y Pan Y Kuang F Zhao B Zhang X X Zhang E X Wu B Xu J Am Chem Soc 2008 130 11828 [20] A R Kortan R Hull R L Opila M G Bawendi M L Steigerwald P J Carroll L E Brus JAm Chem Soc 1990 112 1327 [21] X G Peng M C Schlamp A V Kadavanich A P Alivisatos J Am Chem Soc 1997 119 7019 [22] S Kim B Fisher H J Eisler M Bawendi J Am Chem Soc 2003 125 11466[23] H Zeng J Li Z L Wang J P Liu S Sun Nano Lett 2004 4 187 [24] H Zeng S Sun J Li Z L Wang J P Liu Appl Phys Lett 2004 85 792 [25] Z Xu Y Hou S Sun J Am Chem Soc 2007 129 8698 [26] Y H Lin N Kim J Q Wang C J Zhong J Phys Chem B 2005 109 21593 [27] C Wang H Daimon S Sun Nano Lett 2009 9 1493 [28] K W Kwon M Shim J Am Chem Soc 2005 127 10269 [29] H Yu M Chen P M Rice S X Wang R L White S Sun Nano Lett 2005 5 379 [30] J S Choi Y W Jun S I Yeon H C Kim J S Shin J Cheon J Am Chem Soc 2006 128 15982 [31] S H Choi H B Na Y I Park K An S G Kwon Y Jang M Park J Moon J S Son I CSong W K Moon T Hyeon J Am Chem Soc 2008 130 15573 [32] H W Gu R K Zheng X X Zhang B Xu J Am Chem Soc 2004 126 5664
66
37
MFMNP Design
Core Shell
Dumbbell Structure
Multicomponent Hybrid NP
38
39
Polymer Shell
Core Shell
Metal alloy
(FePt)
Magnetic nanolayer
(Fe3O4)
Thermal decomposition
of Fe(acac)3 on FePt
Magnetic NP
(Fe3O4)
Metal alloy
(Au Ag)
(Fe3O4) in Au3+ solution
at reduction condition
40
1 Core Shell structure
41
- Mix of NP during the growth of nanocrystals
- Sol-gel process
Core Shell synthesis
2 Dumbbell structures
42
- Mix of NP during the growth of nanocrystals
- Thermal rearrangement
- self-assemble at a liquid-liquid interface
- Functional sol-gel matrix
3 Multicomponent Hybrid NPs
Self-assembly processes provide an approach to
fabricating multicomponent hybrid NPs with
integrated multifunctionality
43
- Surfactant polymers
- Sol-gel process
44
Poly(lactico-co-glycolicacid)
(PLGA)
Poly(L-lysine)-PEG-folate
(PLL-PEG-FOL)
By mechanical
stirring at RT
in CH3Cl
45
46
W Lu SR Arumugam D Senapati ACSNANO 2010 4(3) 1739ndash1749
GNP GNP
GNP
RNA Aptamer
Anti-HER2C-reb-2 antibody
GNPCTAB
47
Magnetic nanoparticle surface modification Glucose oxidasse
(GOx)
PEGGPS
APSMNP
48Z Lin et al Analytica Chimica Acta 818 (2014) 54ndash60
49
P Sun H Zhang C Liu Langmuir 2010 26(2) 1278ndash1284 50
Immuno-labeling using Fe3O4CdTe nanocomposites which are formed by
linking multiple TGA-stabilized CdTe QDs with the thiol-functionalized silica
coated iron oxide nanoparticles
23-dimercaptosuccinic
acids (DMSA)Dopamine
Mercaptoundecanoic acid
(MUA)
HS COOH
4 Small multifunctional molecules
Glutaraldehyde 51
C J Xu K M Xu H W Gu R K Zheng H Liu X X Zhang Z H Guo B Xu J Am Chem Soc 2004 126 9938
Dopamine
Conditions (a) NaOH tert-butyl dicarbonate dioxane H2O 24 hrs (b) BnBr K2CO3 DMF
RT 24 h (c) 10 CF3COOH CH2Cl2 rt 5 h (d) succinic anhydride pyridine RTt 3 h (e)
NHS DCC DMAP CHCl3 RTt 3 h
NTA
52
3-mercapto trimethoxysilane
(MPS)
3-amino trimethoxysilane
(APS)
5 Silane multifunctional monomers
Glycidoxypropyltrimethoxy-silane
(GPS)
53
54
Multifunctional magnetic nanoparticles
application
Bioseparation
Biodetection(Real time monitoring)
Targeted drug delivery
Magnetic fluid Hyperthermia therapy
55
1 Specific Bio-Separation
bull Magnetic properties
bull Targeting agent
Cell organism molecule
56
His-tagged protein purification as
the first step towards recombinant
protein purification
58
Lysing Binding
Nickel
affinity
nanoparicle
His tag
fusion protein
Washing Elusion
Digestion
Binding
Nickel
affinity
nanoparticle
Washing Target
Recombinant
Protein
1- Preparation of Fe3O4SiO2Layered Double Hydroxide CoreminusShell
Microspheres for Magnetic Separation of Proteins J Am ChemSoc (2012)
18 134(2) 1071-7
Ni2+
2- Magnetic Nanocomposite Spheres Decorated with NiO Nanoparticles for a
Magnetically Recyclable Protein Separation System J Kim Y Piao N Lee Y
Park I-H Lee J-H Lee Adv Mater (2010) 22 57ndash60
His-tagged protein purification
Purification of His6-lipase using Fe3O4AundashLAndashIDAndashCu2+ NPs with various
amounts of NPs (lane 1 01 mg lane 2 02 mg lane 3
63
Synthetic procedure for the coreshell structure spheres
64
glutaraldehyde
(GLH)
Reference[1] H W Gu Z M Yang J H Gao C K Chang B Xu J Am Chem Soc 2005 127 34
[2] A Hutten D Sudfeld I Ennen G Reiss K Wojczykowski P Jutzi J Magn Magn Mater 2005
293 93
[3] G S Chaubey C Barcena N Poudyal C B Rong J M Gao S Sun J P Liu J Am Chem
Soc 2007 129 7214
[4] C W Kim Y H Kim H G Cha D K Lee Y S Kang J NanosciNanotechnol 2006 6 3417
[5] Y W Jun J S Choi J Cheon Angew Chem Int Ed 2006 45 3414
[6] X Michalet F F Pinaud L A Bentolila J M Tsay S Doose J J Li G Sundaresan A M Wu
S S Gambhir S Weiss Science 2005 307 538
[7] M Hu J Y Chen Z Y Li L Au G V Hartland X D Li M Marquez Y N Xia Chem Soc
Rev 2006 35 1084
[8] M Vallet-Regi F Balas D Arcos Angew Chem Int Ed 2007 46 7548
[9] S T Selvan P K Patra C Y Ang J Y Ying Angew Chem Int Ed 2007 46 2448
[10] I S Lee N Lee J Park B H Kim Y W Yi T Kim T K Kim I H Lee S R Paik T
Hyeon J Am Chem Soc 2006 128 10658
[11] Y S Lin S H Wu Y Hung Y H Chou C Chang M L Lin C P Tsai C Y Mou Chem
Mater 2006 18 5170
[12] B H Wu H Zhang C Chen S C Lin N F Zheng Nano Res 2009 2 975
[13] J Kim J E Lee J Lee J H Yu B C Kim K An Y Hwang C Shin J G Park J Kim T
Hyeon J Am Chem Soc 2006 128 688
[14] S Santra H S Yang P H Holloway J T Stanley R A Mericle J Am Chem Soc 2005 127
1656
[15] D K Yi S T Selvan S S Lee G C Papaefthymiou D Kundaliya J Y Ying J Am Chem
Soc 2005 127 4990 65
[16] J H Gao B Zhang Y Gao Y Pan X X Zhang B Xu J Am Chem Soc 2007 129 11928 [17] J H Gao W Zhang P B Huang B Zhang X X Zhang B Xu J Am Chem Soc 2008 130 3710 [18] J H Gao G L Liang B Zhang Y Kuang X X Zhang B Xu J Am Chem Soc 2007 129 1428 [19] J H Gao G L Liang J S Cheung Y Pan Y Kuang F Zhao B Zhang X X Zhang E X Wu B Xu J Am Chem Soc 2008 130 11828 [20] A R Kortan R Hull R L Opila M G Bawendi M L Steigerwald P J Carroll L E Brus JAm Chem Soc 1990 112 1327 [21] X G Peng M C Schlamp A V Kadavanich A P Alivisatos J Am Chem Soc 1997 119 7019 [22] S Kim B Fisher H J Eisler M Bawendi J Am Chem Soc 2003 125 11466[23] H Zeng J Li Z L Wang J P Liu S Sun Nano Lett 2004 4 187 [24] H Zeng S Sun J Li Z L Wang J P Liu Appl Phys Lett 2004 85 792 [25] Z Xu Y Hou S Sun J Am Chem Soc 2007 129 8698 [26] Y H Lin N Kim J Q Wang C J Zhong J Phys Chem B 2005 109 21593 [27] C Wang H Daimon S Sun Nano Lett 2009 9 1493 [28] K W Kwon M Shim J Am Chem Soc 2005 127 10269 [29] H Yu M Chen P M Rice S X Wang R L White S Sun Nano Lett 2005 5 379 [30] J S Choi Y W Jun S I Yeon H C Kim J S Shin J Cheon J Am Chem Soc 2006 128 15982 [31] S H Choi H B Na Y I Park K An S G Kwon Y Jang M Park J Moon J S Son I CSong W K Moon T Hyeon J Am Chem Soc 2008 130 15573 [32] H W Gu R K Zheng X X Zhang B Xu J Am Chem Soc 2004 126 5664
66
MFMNP Design
Core Shell
Dumbbell Structure
Multicomponent Hybrid NP
38
39
Polymer Shell
Core Shell
Metal alloy
(FePt)
Magnetic nanolayer
(Fe3O4)
Thermal decomposition
of Fe(acac)3 on FePt
Magnetic NP
(Fe3O4)
Metal alloy
(Au Ag)
(Fe3O4) in Au3+ solution
at reduction condition
40
1 Core Shell structure
41
- Mix of NP during the growth of nanocrystals
- Sol-gel process
Core Shell synthesis
2 Dumbbell structures
42
- Mix of NP during the growth of nanocrystals
- Thermal rearrangement
- self-assemble at a liquid-liquid interface
- Functional sol-gel matrix
3 Multicomponent Hybrid NPs
Self-assembly processes provide an approach to
fabricating multicomponent hybrid NPs with
integrated multifunctionality
43
- Surfactant polymers
- Sol-gel process
44
Poly(lactico-co-glycolicacid)
(PLGA)
Poly(L-lysine)-PEG-folate
(PLL-PEG-FOL)
By mechanical
stirring at RT
in CH3Cl
45
46
W Lu SR Arumugam D Senapati ACSNANO 2010 4(3) 1739ndash1749
GNP GNP
GNP
RNA Aptamer
Anti-HER2C-reb-2 antibody
GNPCTAB
47
Magnetic nanoparticle surface modification Glucose oxidasse
(GOx)
PEGGPS
APSMNP
48Z Lin et al Analytica Chimica Acta 818 (2014) 54ndash60
49
P Sun H Zhang C Liu Langmuir 2010 26(2) 1278ndash1284 50
Immuno-labeling using Fe3O4CdTe nanocomposites which are formed by
linking multiple TGA-stabilized CdTe QDs with the thiol-functionalized silica
coated iron oxide nanoparticles
23-dimercaptosuccinic
acids (DMSA)Dopamine
Mercaptoundecanoic acid
(MUA)
HS COOH
4 Small multifunctional molecules
Glutaraldehyde 51
C J Xu K M Xu H W Gu R K Zheng H Liu X X Zhang Z H Guo B Xu J Am Chem Soc 2004 126 9938
Dopamine
Conditions (a) NaOH tert-butyl dicarbonate dioxane H2O 24 hrs (b) BnBr K2CO3 DMF
RT 24 h (c) 10 CF3COOH CH2Cl2 rt 5 h (d) succinic anhydride pyridine RTt 3 h (e)
NHS DCC DMAP CHCl3 RTt 3 h
NTA
52
3-mercapto trimethoxysilane
(MPS)
3-amino trimethoxysilane
(APS)
5 Silane multifunctional monomers
Glycidoxypropyltrimethoxy-silane
(GPS)
53
54
Multifunctional magnetic nanoparticles
application
Bioseparation
Biodetection(Real time monitoring)
Targeted drug delivery
Magnetic fluid Hyperthermia therapy
55
1 Specific Bio-Separation
bull Magnetic properties
bull Targeting agent
Cell organism molecule
56
His-tagged protein purification as
the first step towards recombinant
protein purification
58
Lysing Binding
Nickel
affinity
nanoparicle
His tag
fusion protein
Washing Elusion
Digestion
Binding
Nickel
affinity
nanoparticle
Washing Target
Recombinant
Protein
1- Preparation of Fe3O4SiO2Layered Double Hydroxide CoreminusShell
Microspheres for Magnetic Separation of Proteins J Am ChemSoc (2012)
18 134(2) 1071-7
Ni2+
2- Magnetic Nanocomposite Spheres Decorated with NiO Nanoparticles for a
Magnetically Recyclable Protein Separation System J Kim Y Piao N Lee Y
Park I-H Lee J-H Lee Adv Mater (2010) 22 57ndash60
His-tagged protein purification
Purification of His6-lipase using Fe3O4AundashLAndashIDAndashCu2+ NPs with various
amounts of NPs (lane 1 01 mg lane 2 02 mg lane 3
63
Synthetic procedure for the coreshell structure spheres
64
glutaraldehyde
(GLH)
Reference[1] H W Gu Z M Yang J H Gao C K Chang B Xu J Am Chem Soc 2005 127 34
[2] A Hutten D Sudfeld I Ennen G Reiss K Wojczykowski P Jutzi J Magn Magn Mater 2005
293 93
[3] G S Chaubey C Barcena N Poudyal C B Rong J M Gao S Sun J P Liu J Am Chem
Soc 2007 129 7214
[4] C W Kim Y H Kim H G Cha D K Lee Y S Kang J NanosciNanotechnol 2006 6 3417
[5] Y W Jun J S Choi J Cheon Angew Chem Int Ed 2006 45 3414
[6] X Michalet F F Pinaud L A Bentolila J M Tsay S Doose J J Li G Sundaresan A M Wu
S S Gambhir S Weiss Science 2005 307 538
[7] M Hu J Y Chen Z Y Li L Au G V Hartland X D Li M Marquez Y N Xia Chem Soc
Rev 2006 35 1084
[8] M Vallet-Regi F Balas D Arcos Angew Chem Int Ed 2007 46 7548
[9] S T Selvan P K Patra C Y Ang J Y Ying Angew Chem Int Ed 2007 46 2448
[10] I S Lee N Lee J Park B H Kim Y W Yi T Kim T K Kim I H Lee S R Paik T
Hyeon J Am Chem Soc 2006 128 10658
[11] Y S Lin S H Wu Y Hung Y H Chou C Chang M L Lin C P Tsai C Y Mou Chem
Mater 2006 18 5170
[12] B H Wu H Zhang C Chen S C Lin N F Zheng Nano Res 2009 2 975
[13] J Kim J E Lee J Lee J H Yu B C Kim K An Y Hwang C Shin J G Park J Kim T
Hyeon J Am Chem Soc 2006 128 688
[14] S Santra H S Yang P H Holloway J T Stanley R A Mericle J Am Chem Soc 2005 127
1656
[15] D K Yi S T Selvan S S Lee G C Papaefthymiou D Kundaliya J Y Ying J Am Chem
Soc 2005 127 4990 65
[16] J H Gao B Zhang Y Gao Y Pan X X Zhang B Xu J Am Chem Soc 2007 129 11928 [17] J H Gao W Zhang P B Huang B Zhang X X Zhang B Xu J Am Chem Soc 2008 130 3710 [18] J H Gao G L Liang B Zhang Y Kuang X X Zhang B Xu J Am Chem Soc 2007 129 1428 [19] J H Gao G L Liang J S Cheung Y Pan Y Kuang F Zhao B Zhang X X Zhang E X Wu B Xu J Am Chem Soc 2008 130 11828 [20] A R Kortan R Hull R L Opila M G Bawendi M L Steigerwald P J Carroll L E Brus JAm Chem Soc 1990 112 1327 [21] X G Peng M C Schlamp A V Kadavanich A P Alivisatos J Am Chem Soc 1997 119 7019 [22] S Kim B Fisher H J Eisler M Bawendi J Am Chem Soc 2003 125 11466[23] H Zeng J Li Z L Wang J P Liu S Sun Nano Lett 2004 4 187 [24] H Zeng S Sun J Li Z L Wang J P Liu Appl Phys Lett 2004 85 792 [25] Z Xu Y Hou S Sun J Am Chem Soc 2007 129 8698 [26] Y H Lin N Kim J Q Wang C J Zhong J Phys Chem B 2005 109 21593 [27] C Wang H Daimon S Sun Nano Lett 2009 9 1493 [28] K W Kwon M Shim J Am Chem Soc 2005 127 10269 [29] H Yu M Chen P M Rice S X Wang R L White S Sun Nano Lett 2005 5 379 [30] J S Choi Y W Jun S I Yeon H C Kim J S Shin J Cheon J Am Chem Soc 2006 128 15982 [31] S H Choi H B Na Y I Park K An S G Kwon Y Jang M Park J Moon J S Son I CSong W K Moon T Hyeon J Am Chem Soc 2008 130 15573 [32] H W Gu R K Zheng X X Zhang B Xu J Am Chem Soc 2004 126 5664
66
39
Polymer Shell
Core Shell
Metal alloy
(FePt)
Magnetic nanolayer
(Fe3O4)
Thermal decomposition
of Fe(acac)3 on FePt
Magnetic NP
(Fe3O4)
Metal alloy
(Au Ag)
(Fe3O4) in Au3+ solution
at reduction condition
40
1 Core Shell structure
41
- Mix of NP during the growth of nanocrystals
- Sol-gel process
Core Shell synthesis
2 Dumbbell structures
42
- Mix of NP during the growth of nanocrystals
- Thermal rearrangement
- self-assemble at a liquid-liquid interface
- Functional sol-gel matrix
3 Multicomponent Hybrid NPs
Self-assembly processes provide an approach to
fabricating multicomponent hybrid NPs with
integrated multifunctionality
43
- Surfactant polymers
- Sol-gel process
44
Poly(lactico-co-glycolicacid)
(PLGA)
Poly(L-lysine)-PEG-folate
(PLL-PEG-FOL)
By mechanical
stirring at RT
in CH3Cl
45
46
W Lu SR Arumugam D Senapati ACSNANO 2010 4(3) 1739ndash1749
GNP GNP
GNP
RNA Aptamer
Anti-HER2C-reb-2 antibody
GNPCTAB
47
Magnetic nanoparticle surface modification Glucose oxidasse
(GOx)
PEGGPS
APSMNP
48Z Lin et al Analytica Chimica Acta 818 (2014) 54ndash60
49
P Sun H Zhang C Liu Langmuir 2010 26(2) 1278ndash1284 50
Immuno-labeling using Fe3O4CdTe nanocomposites which are formed by
linking multiple TGA-stabilized CdTe QDs with the thiol-functionalized silica
coated iron oxide nanoparticles
23-dimercaptosuccinic
acids (DMSA)Dopamine
Mercaptoundecanoic acid
(MUA)
HS COOH
4 Small multifunctional molecules
Glutaraldehyde 51
C J Xu K M Xu H W Gu R K Zheng H Liu X X Zhang Z H Guo B Xu J Am Chem Soc 2004 126 9938
Dopamine
Conditions (a) NaOH tert-butyl dicarbonate dioxane H2O 24 hrs (b) BnBr K2CO3 DMF
RT 24 h (c) 10 CF3COOH CH2Cl2 rt 5 h (d) succinic anhydride pyridine RTt 3 h (e)
NHS DCC DMAP CHCl3 RTt 3 h
NTA
52
3-mercapto trimethoxysilane
(MPS)
3-amino trimethoxysilane
(APS)
5 Silane multifunctional monomers
Glycidoxypropyltrimethoxy-silane
(GPS)
53
54
Multifunctional magnetic nanoparticles
application
Bioseparation
Biodetection(Real time monitoring)
Targeted drug delivery
Magnetic fluid Hyperthermia therapy
55
1 Specific Bio-Separation
bull Magnetic properties
bull Targeting agent
Cell organism molecule
56
His-tagged protein purification as
the first step towards recombinant
protein purification
58
Lysing Binding
Nickel
affinity
nanoparicle
His tag
fusion protein
Washing Elusion
Digestion
Binding
Nickel
affinity
nanoparticle
Washing Target
Recombinant
Protein
1- Preparation of Fe3O4SiO2Layered Double Hydroxide CoreminusShell
Microspheres for Magnetic Separation of Proteins J Am ChemSoc (2012)
18 134(2) 1071-7
Ni2+
2- Magnetic Nanocomposite Spheres Decorated with NiO Nanoparticles for a
Magnetically Recyclable Protein Separation System J Kim Y Piao N Lee Y
Park I-H Lee J-H Lee Adv Mater (2010) 22 57ndash60
His-tagged protein purification
Purification of His6-lipase using Fe3O4AundashLAndashIDAndashCu2+ NPs with various
amounts of NPs (lane 1 01 mg lane 2 02 mg lane 3
63
Synthetic procedure for the coreshell structure spheres
64
glutaraldehyde
(GLH)
Reference[1] H W Gu Z M Yang J H Gao C K Chang B Xu J Am Chem Soc 2005 127 34
[2] A Hutten D Sudfeld I Ennen G Reiss K Wojczykowski P Jutzi J Magn Magn Mater 2005
293 93
[3] G S Chaubey C Barcena N Poudyal C B Rong J M Gao S Sun J P Liu J Am Chem
Soc 2007 129 7214
[4] C W Kim Y H Kim H G Cha D K Lee Y S Kang J NanosciNanotechnol 2006 6 3417
[5] Y W Jun J S Choi J Cheon Angew Chem Int Ed 2006 45 3414
[6] X Michalet F F Pinaud L A Bentolila J M Tsay S Doose J J Li G Sundaresan A M Wu
S S Gambhir S Weiss Science 2005 307 538
[7] M Hu J Y Chen Z Y Li L Au G V Hartland X D Li M Marquez Y N Xia Chem Soc
Rev 2006 35 1084
[8] M Vallet-Regi F Balas D Arcos Angew Chem Int Ed 2007 46 7548
[9] S T Selvan P K Patra C Y Ang J Y Ying Angew Chem Int Ed 2007 46 2448
[10] I S Lee N Lee J Park B H Kim Y W Yi T Kim T K Kim I H Lee S R Paik T
Hyeon J Am Chem Soc 2006 128 10658
[11] Y S Lin S H Wu Y Hung Y H Chou C Chang M L Lin C P Tsai C Y Mou Chem
Mater 2006 18 5170
[12] B H Wu H Zhang C Chen S C Lin N F Zheng Nano Res 2009 2 975
[13] J Kim J E Lee J Lee J H Yu B C Kim K An Y Hwang C Shin J G Park J Kim T
Hyeon J Am Chem Soc 2006 128 688
[14] S Santra H S Yang P H Holloway J T Stanley R A Mericle J Am Chem Soc 2005 127
1656
[15] D K Yi S T Selvan S S Lee G C Papaefthymiou D Kundaliya J Y Ying J Am Chem
Soc 2005 127 4990 65
[16] J H Gao B Zhang Y Gao Y Pan X X Zhang B Xu J Am Chem Soc 2007 129 11928 [17] J H Gao W Zhang P B Huang B Zhang X X Zhang B Xu J Am Chem Soc 2008 130 3710 [18] J H Gao G L Liang B Zhang Y Kuang X X Zhang B Xu J Am Chem Soc 2007 129 1428 [19] J H Gao G L Liang J S Cheung Y Pan Y Kuang F Zhao B Zhang X X Zhang E X Wu B Xu J Am Chem Soc 2008 130 11828 [20] A R Kortan R Hull R L Opila M G Bawendi M L Steigerwald P J Carroll L E Brus JAm Chem Soc 1990 112 1327 [21] X G Peng M C Schlamp A V Kadavanich A P Alivisatos J Am Chem Soc 1997 119 7019 [22] S Kim B Fisher H J Eisler M Bawendi J Am Chem Soc 2003 125 11466[23] H Zeng J Li Z L Wang J P Liu S Sun Nano Lett 2004 4 187 [24] H Zeng S Sun J Li Z L Wang J P Liu Appl Phys Lett 2004 85 792 [25] Z Xu Y Hou S Sun J Am Chem Soc 2007 129 8698 [26] Y H Lin N Kim J Q Wang C J Zhong J Phys Chem B 2005 109 21593 [27] C Wang H Daimon S Sun Nano Lett 2009 9 1493 [28] K W Kwon M Shim J Am Chem Soc 2005 127 10269 [29] H Yu M Chen P M Rice S X Wang R L White S Sun Nano Lett 2005 5 379 [30] J S Choi Y W Jun S I Yeon H C Kim J S Shin J Cheon J Am Chem Soc 2006 128 15982 [31] S H Choi H B Na Y I Park K An S G Kwon Y Jang M Park J Moon J S Son I CSong W K Moon T Hyeon J Am Chem Soc 2008 130 15573 [32] H W Gu R K Zheng X X Zhang B Xu J Am Chem Soc 2004 126 5664
66
Core Shell
Metal alloy
(FePt)
Magnetic nanolayer
(Fe3O4)
Thermal decomposition
of Fe(acac)3 on FePt
Magnetic NP
(Fe3O4)
Metal alloy
(Au Ag)
(Fe3O4) in Au3+ solution
at reduction condition
40
1 Core Shell structure
41
- Mix of NP during the growth of nanocrystals
- Sol-gel process
Core Shell synthesis
2 Dumbbell structures
42
- Mix of NP during the growth of nanocrystals
- Thermal rearrangement
- self-assemble at a liquid-liquid interface
- Functional sol-gel matrix
3 Multicomponent Hybrid NPs
Self-assembly processes provide an approach to
fabricating multicomponent hybrid NPs with
integrated multifunctionality
43
- Surfactant polymers
- Sol-gel process
44
Poly(lactico-co-glycolicacid)
(PLGA)
Poly(L-lysine)-PEG-folate
(PLL-PEG-FOL)
By mechanical
stirring at RT
in CH3Cl
45
46
W Lu SR Arumugam D Senapati ACSNANO 2010 4(3) 1739ndash1749
GNP GNP
GNP
RNA Aptamer
Anti-HER2C-reb-2 antibody
GNPCTAB
47
Magnetic nanoparticle surface modification Glucose oxidasse
(GOx)
PEGGPS
APSMNP
48Z Lin et al Analytica Chimica Acta 818 (2014) 54ndash60
49
P Sun H Zhang C Liu Langmuir 2010 26(2) 1278ndash1284 50
Immuno-labeling using Fe3O4CdTe nanocomposites which are formed by
linking multiple TGA-stabilized CdTe QDs with the thiol-functionalized silica
coated iron oxide nanoparticles
23-dimercaptosuccinic
acids (DMSA)Dopamine
Mercaptoundecanoic acid
(MUA)
HS COOH
4 Small multifunctional molecules
Glutaraldehyde 51
C J Xu K M Xu H W Gu R K Zheng H Liu X X Zhang Z H Guo B Xu J Am Chem Soc 2004 126 9938
Dopamine
Conditions (a) NaOH tert-butyl dicarbonate dioxane H2O 24 hrs (b) BnBr K2CO3 DMF
RT 24 h (c) 10 CF3COOH CH2Cl2 rt 5 h (d) succinic anhydride pyridine RTt 3 h (e)
NHS DCC DMAP CHCl3 RTt 3 h
NTA
52
3-mercapto trimethoxysilane
(MPS)
3-amino trimethoxysilane
(APS)
5 Silane multifunctional monomers
Glycidoxypropyltrimethoxy-silane
(GPS)
53
54
Multifunctional magnetic nanoparticles
application
Bioseparation
Biodetection(Real time monitoring)
Targeted drug delivery
Magnetic fluid Hyperthermia therapy
55
1 Specific Bio-Separation
bull Magnetic properties
bull Targeting agent
Cell organism molecule
56
His-tagged protein purification as
the first step towards recombinant
protein purification
58
Lysing Binding
Nickel
affinity
nanoparicle
His tag
fusion protein
Washing Elusion
Digestion
Binding
Nickel
affinity
nanoparticle
Washing Target
Recombinant
Protein
1- Preparation of Fe3O4SiO2Layered Double Hydroxide CoreminusShell
Microspheres for Magnetic Separation of Proteins J Am ChemSoc (2012)
18 134(2) 1071-7
Ni2+
2- Magnetic Nanocomposite Spheres Decorated with NiO Nanoparticles for a
Magnetically Recyclable Protein Separation System J Kim Y Piao N Lee Y
Park I-H Lee J-H Lee Adv Mater (2010) 22 57ndash60
His-tagged protein purification
Purification of His6-lipase using Fe3O4AundashLAndashIDAndashCu2+ NPs with various
amounts of NPs (lane 1 01 mg lane 2 02 mg lane 3
63
Synthetic procedure for the coreshell structure spheres
64
glutaraldehyde
(GLH)
Reference[1] H W Gu Z M Yang J H Gao C K Chang B Xu J Am Chem Soc 2005 127 34
[2] A Hutten D Sudfeld I Ennen G Reiss K Wojczykowski P Jutzi J Magn Magn Mater 2005
293 93
[3] G S Chaubey C Barcena N Poudyal C B Rong J M Gao S Sun J P Liu J Am Chem
Soc 2007 129 7214
[4] C W Kim Y H Kim H G Cha D K Lee Y S Kang J NanosciNanotechnol 2006 6 3417
[5] Y W Jun J S Choi J Cheon Angew Chem Int Ed 2006 45 3414
[6] X Michalet F F Pinaud L A Bentolila J M Tsay S Doose J J Li G Sundaresan A M Wu
S S Gambhir S Weiss Science 2005 307 538
[7] M Hu J Y Chen Z Y Li L Au G V Hartland X D Li M Marquez Y N Xia Chem Soc
Rev 2006 35 1084
[8] M Vallet-Regi F Balas D Arcos Angew Chem Int Ed 2007 46 7548
[9] S T Selvan P K Patra C Y Ang J Y Ying Angew Chem Int Ed 2007 46 2448
[10] I S Lee N Lee J Park B H Kim Y W Yi T Kim T K Kim I H Lee S R Paik T
Hyeon J Am Chem Soc 2006 128 10658
[11] Y S Lin S H Wu Y Hung Y H Chou C Chang M L Lin C P Tsai C Y Mou Chem
Mater 2006 18 5170
[12] B H Wu H Zhang C Chen S C Lin N F Zheng Nano Res 2009 2 975
[13] J Kim J E Lee J Lee J H Yu B C Kim K An Y Hwang C Shin J G Park J Kim T
Hyeon J Am Chem Soc 2006 128 688
[14] S Santra H S Yang P H Holloway J T Stanley R A Mericle J Am Chem Soc 2005 127
1656
[15] D K Yi S T Selvan S S Lee G C Papaefthymiou D Kundaliya J Y Ying J Am Chem
Soc 2005 127 4990 65
[16] J H Gao B Zhang Y Gao Y Pan X X Zhang B Xu J Am Chem Soc 2007 129 11928 [17] J H Gao W Zhang P B Huang B Zhang X X Zhang B Xu J Am Chem Soc 2008 130 3710 [18] J H Gao G L Liang B Zhang Y Kuang X X Zhang B Xu J Am Chem Soc 2007 129 1428 [19] J H Gao G L Liang J S Cheung Y Pan Y Kuang F Zhao B Zhang X X Zhang E X Wu B Xu J Am Chem Soc 2008 130 11828 [20] A R Kortan R Hull R L Opila M G Bawendi M L Steigerwald P J Carroll L E Brus JAm Chem Soc 1990 112 1327 [21] X G Peng M C Schlamp A V Kadavanich A P Alivisatos J Am Chem Soc 1997 119 7019 [22] S Kim B Fisher H J Eisler M Bawendi J Am Chem Soc 2003 125 11466[23] H Zeng J Li Z L Wang J P Liu S Sun Nano Lett 2004 4 187 [24] H Zeng S Sun J Li Z L Wang J P Liu Appl Phys Lett 2004 85 792 [25] Z Xu Y Hou S Sun J Am Chem Soc 2007 129 8698 [26] Y H Lin N Kim J Q Wang C J Zhong J Phys Chem B 2005 109 21593 [27] C Wang H Daimon S Sun Nano Lett 2009 9 1493 [28] K W Kwon M Shim J Am Chem Soc 2005 127 10269 [29] H Yu M Chen P M Rice S X Wang R L White S Sun Nano Lett 2005 5 379 [30] J S Choi Y W Jun S I Yeon H C Kim J S Shin J Cheon J Am Chem Soc 2006 128 15982 [31] S H Choi H B Na Y I Park K An S G Kwon Y Jang M Park J Moon J S Son I CSong W K Moon T Hyeon J Am Chem Soc 2008 130 15573 [32] H W Gu R K Zheng X X Zhang B Xu J Am Chem Soc 2004 126 5664
66
41
- Mix of NP during the growth of nanocrystals
- Sol-gel process
Core Shell synthesis
2 Dumbbell structures
42
- Mix of NP during the growth of nanocrystals
- Thermal rearrangement
- self-assemble at a liquid-liquid interface
- Functional sol-gel matrix
3 Multicomponent Hybrid NPs
Self-assembly processes provide an approach to
fabricating multicomponent hybrid NPs with
integrated multifunctionality
43
- Surfactant polymers
- Sol-gel process
44
Poly(lactico-co-glycolicacid)
(PLGA)
Poly(L-lysine)-PEG-folate
(PLL-PEG-FOL)
By mechanical
stirring at RT
in CH3Cl
45
46
W Lu SR Arumugam D Senapati ACSNANO 2010 4(3) 1739ndash1749
GNP GNP
GNP
RNA Aptamer
Anti-HER2C-reb-2 antibody
GNPCTAB
47
Magnetic nanoparticle surface modification Glucose oxidasse
(GOx)
PEGGPS
APSMNP
48Z Lin et al Analytica Chimica Acta 818 (2014) 54ndash60
49
P Sun H Zhang C Liu Langmuir 2010 26(2) 1278ndash1284 50
Immuno-labeling using Fe3O4CdTe nanocomposites which are formed by
linking multiple TGA-stabilized CdTe QDs with the thiol-functionalized silica
coated iron oxide nanoparticles
23-dimercaptosuccinic
acids (DMSA)Dopamine
Mercaptoundecanoic acid
(MUA)
HS COOH
4 Small multifunctional molecules
Glutaraldehyde 51
C J Xu K M Xu H W Gu R K Zheng H Liu X X Zhang Z H Guo B Xu J Am Chem Soc 2004 126 9938
Dopamine
Conditions (a) NaOH tert-butyl dicarbonate dioxane H2O 24 hrs (b) BnBr K2CO3 DMF
RT 24 h (c) 10 CF3COOH CH2Cl2 rt 5 h (d) succinic anhydride pyridine RTt 3 h (e)
NHS DCC DMAP CHCl3 RTt 3 h
NTA
52
3-mercapto trimethoxysilane
(MPS)
3-amino trimethoxysilane
(APS)
5 Silane multifunctional monomers
Glycidoxypropyltrimethoxy-silane
(GPS)
53
54
Multifunctional magnetic nanoparticles
application
Bioseparation
Biodetection(Real time monitoring)
Targeted drug delivery
Magnetic fluid Hyperthermia therapy
55
1 Specific Bio-Separation
bull Magnetic properties
bull Targeting agent
Cell organism molecule
56
His-tagged protein purification as
the first step towards recombinant
protein purification
58
Lysing Binding
Nickel
affinity
nanoparicle
His tag
fusion protein
Washing Elusion
Digestion
Binding
Nickel
affinity
nanoparticle
Washing Target
Recombinant
Protein
1- Preparation of Fe3O4SiO2Layered Double Hydroxide CoreminusShell
Microspheres for Magnetic Separation of Proteins J Am ChemSoc (2012)
18 134(2) 1071-7
Ni2+
2- Magnetic Nanocomposite Spheres Decorated with NiO Nanoparticles for a
Magnetically Recyclable Protein Separation System J Kim Y Piao N Lee Y
Park I-H Lee J-H Lee Adv Mater (2010) 22 57ndash60
His-tagged protein purification
Purification of His6-lipase using Fe3O4AundashLAndashIDAndashCu2+ NPs with various
amounts of NPs (lane 1 01 mg lane 2 02 mg lane 3
63
Synthetic procedure for the coreshell structure spheres
64
glutaraldehyde
(GLH)
Reference[1] H W Gu Z M Yang J H Gao C K Chang B Xu J Am Chem Soc 2005 127 34
[2] A Hutten D Sudfeld I Ennen G Reiss K Wojczykowski P Jutzi J Magn Magn Mater 2005
293 93
[3] G S Chaubey C Barcena N Poudyal C B Rong J M Gao S Sun J P Liu J Am Chem
Soc 2007 129 7214
[4] C W Kim Y H Kim H G Cha D K Lee Y S Kang J NanosciNanotechnol 2006 6 3417
[5] Y W Jun J S Choi J Cheon Angew Chem Int Ed 2006 45 3414
[6] X Michalet F F Pinaud L A Bentolila J M Tsay S Doose J J Li G Sundaresan A M Wu
S S Gambhir S Weiss Science 2005 307 538
[7] M Hu J Y Chen Z Y Li L Au G V Hartland X D Li M Marquez Y N Xia Chem Soc
Rev 2006 35 1084
[8] M Vallet-Regi F Balas D Arcos Angew Chem Int Ed 2007 46 7548
[9] S T Selvan P K Patra C Y Ang J Y Ying Angew Chem Int Ed 2007 46 2448
[10] I S Lee N Lee J Park B H Kim Y W Yi T Kim T K Kim I H Lee S R Paik T
Hyeon J Am Chem Soc 2006 128 10658
[11] Y S Lin S H Wu Y Hung Y H Chou C Chang M L Lin C P Tsai C Y Mou Chem
Mater 2006 18 5170
[12] B H Wu H Zhang C Chen S C Lin N F Zheng Nano Res 2009 2 975
[13] J Kim J E Lee J Lee J H Yu B C Kim K An Y Hwang C Shin J G Park J Kim T
Hyeon J Am Chem Soc 2006 128 688
[14] S Santra H S Yang P H Holloway J T Stanley R A Mericle J Am Chem Soc 2005 127
1656
[15] D K Yi S T Selvan S S Lee G C Papaefthymiou D Kundaliya J Y Ying J Am Chem
Soc 2005 127 4990 65
[16] J H Gao B Zhang Y Gao Y Pan X X Zhang B Xu J Am Chem Soc 2007 129 11928 [17] J H Gao W Zhang P B Huang B Zhang X X Zhang B Xu J Am Chem Soc 2008 130 3710 [18] J H Gao G L Liang B Zhang Y Kuang X X Zhang B Xu J Am Chem Soc 2007 129 1428 [19] J H Gao G L Liang J S Cheung Y Pan Y Kuang F Zhao B Zhang X X Zhang E X Wu B Xu J Am Chem Soc 2008 130 11828 [20] A R Kortan R Hull R L Opila M G Bawendi M L Steigerwald P J Carroll L E Brus JAm Chem Soc 1990 112 1327 [21] X G Peng M C Schlamp A V Kadavanich A P Alivisatos J Am Chem Soc 1997 119 7019 [22] S Kim B Fisher H J Eisler M Bawendi J Am Chem Soc 2003 125 11466[23] H Zeng J Li Z L Wang J P Liu S Sun Nano Lett 2004 4 187 [24] H Zeng S Sun J Li Z L Wang J P Liu Appl Phys Lett 2004 85 792 [25] Z Xu Y Hou S Sun J Am Chem Soc 2007 129 8698 [26] Y H Lin N Kim J Q Wang C J Zhong J Phys Chem B 2005 109 21593 [27] C Wang H Daimon S Sun Nano Lett 2009 9 1493 [28] K W Kwon M Shim J Am Chem Soc 2005 127 10269 [29] H Yu M Chen P M Rice S X Wang R L White S Sun Nano Lett 2005 5 379 [30] J S Choi Y W Jun S I Yeon H C Kim J S Shin J Cheon J Am Chem Soc 2006 128 15982 [31] S H Choi H B Na Y I Park K An S G Kwon Y Jang M Park J Moon J S Son I CSong W K Moon T Hyeon J Am Chem Soc 2008 130 15573 [32] H W Gu R K Zheng X X Zhang B Xu J Am Chem Soc 2004 126 5664
66
2 Dumbbell structures
42
- Mix of NP during the growth of nanocrystals
- Thermal rearrangement
- self-assemble at a liquid-liquid interface
- Functional sol-gel matrix
3 Multicomponent Hybrid NPs
Self-assembly processes provide an approach to
fabricating multicomponent hybrid NPs with
integrated multifunctionality
43
- Surfactant polymers
- Sol-gel process
44
Poly(lactico-co-glycolicacid)
(PLGA)
Poly(L-lysine)-PEG-folate
(PLL-PEG-FOL)
By mechanical
stirring at RT
in CH3Cl
45
46
W Lu SR Arumugam D Senapati ACSNANO 2010 4(3) 1739ndash1749
GNP GNP
GNP
RNA Aptamer
Anti-HER2C-reb-2 antibody
GNPCTAB
47
Magnetic nanoparticle surface modification Glucose oxidasse
(GOx)
PEGGPS
APSMNP
48Z Lin et al Analytica Chimica Acta 818 (2014) 54ndash60
49
P Sun H Zhang C Liu Langmuir 2010 26(2) 1278ndash1284 50
Immuno-labeling using Fe3O4CdTe nanocomposites which are formed by
linking multiple TGA-stabilized CdTe QDs with the thiol-functionalized silica
coated iron oxide nanoparticles
23-dimercaptosuccinic
acids (DMSA)Dopamine
Mercaptoundecanoic acid
(MUA)
HS COOH
4 Small multifunctional molecules
Glutaraldehyde 51
C J Xu K M Xu H W Gu R K Zheng H Liu X X Zhang Z H Guo B Xu J Am Chem Soc 2004 126 9938
Dopamine
Conditions (a) NaOH tert-butyl dicarbonate dioxane H2O 24 hrs (b) BnBr K2CO3 DMF
RT 24 h (c) 10 CF3COOH CH2Cl2 rt 5 h (d) succinic anhydride pyridine RTt 3 h (e)
NHS DCC DMAP CHCl3 RTt 3 h
NTA
52
3-mercapto trimethoxysilane
(MPS)
3-amino trimethoxysilane
(APS)
5 Silane multifunctional monomers
Glycidoxypropyltrimethoxy-silane
(GPS)
53
54
Multifunctional magnetic nanoparticles
application
Bioseparation
Biodetection(Real time monitoring)
Targeted drug delivery
Magnetic fluid Hyperthermia therapy
55
1 Specific Bio-Separation
bull Magnetic properties
bull Targeting agent
Cell organism molecule
56
His-tagged protein purification as
the first step towards recombinant
protein purification
58
Lysing Binding
Nickel
affinity
nanoparicle
His tag
fusion protein
Washing Elusion
Digestion
Binding
Nickel
affinity
nanoparticle
Washing Target
Recombinant
Protein
1- Preparation of Fe3O4SiO2Layered Double Hydroxide CoreminusShell
Microspheres for Magnetic Separation of Proteins J Am ChemSoc (2012)
18 134(2) 1071-7
Ni2+
2- Magnetic Nanocomposite Spheres Decorated with NiO Nanoparticles for a
Magnetically Recyclable Protein Separation System J Kim Y Piao N Lee Y
Park I-H Lee J-H Lee Adv Mater (2010) 22 57ndash60
His-tagged protein purification
Purification of His6-lipase using Fe3O4AundashLAndashIDAndashCu2+ NPs with various
amounts of NPs (lane 1 01 mg lane 2 02 mg lane 3
63
Synthetic procedure for the coreshell structure spheres
64
glutaraldehyde
(GLH)
Reference[1] H W Gu Z M Yang J H Gao C K Chang B Xu J Am Chem Soc 2005 127 34
[2] A Hutten D Sudfeld I Ennen G Reiss K Wojczykowski P Jutzi J Magn Magn Mater 2005
293 93
[3] G S Chaubey C Barcena N Poudyal C B Rong J M Gao S Sun J P Liu J Am Chem
Soc 2007 129 7214
[4] C W Kim Y H Kim H G Cha D K Lee Y S Kang J NanosciNanotechnol 2006 6 3417
[5] Y W Jun J S Choi J Cheon Angew Chem Int Ed 2006 45 3414
[6] X Michalet F F Pinaud L A Bentolila J M Tsay S Doose J J Li G Sundaresan A M Wu
S S Gambhir S Weiss Science 2005 307 538
[7] M Hu J Y Chen Z Y Li L Au G V Hartland X D Li M Marquez Y N Xia Chem Soc
Rev 2006 35 1084
[8] M Vallet-Regi F Balas D Arcos Angew Chem Int Ed 2007 46 7548
[9] S T Selvan P K Patra C Y Ang J Y Ying Angew Chem Int Ed 2007 46 2448
[10] I S Lee N Lee J Park B H Kim Y W Yi T Kim T K Kim I H Lee S R Paik T
Hyeon J Am Chem Soc 2006 128 10658
[11] Y S Lin S H Wu Y Hung Y H Chou C Chang M L Lin C P Tsai C Y Mou Chem
Mater 2006 18 5170
[12] B H Wu H Zhang C Chen S C Lin N F Zheng Nano Res 2009 2 975
[13] J Kim J E Lee J Lee J H Yu B C Kim K An Y Hwang C Shin J G Park J Kim T
Hyeon J Am Chem Soc 2006 128 688
[14] S Santra H S Yang P H Holloway J T Stanley R A Mericle J Am Chem Soc 2005 127
1656
[15] D K Yi S T Selvan S S Lee G C Papaefthymiou D Kundaliya J Y Ying J Am Chem
Soc 2005 127 4990 65
[16] J H Gao B Zhang Y Gao Y Pan X X Zhang B Xu J Am Chem Soc 2007 129 11928 [17] J H Gao W Zhang P B Huang B Zhang X X Zhang B Xu J Am Chem Soc 2008 130 3710 [18] J H Gao G L Liang B Zhang Y Kuang X X Zhang B Xu J Am Chem Soc 2007 129 1428 [19] J H Gao G L Liang J S Cheung Y Pan Y Kuang F Zhao B Zhang X X Zhang E X Wu B Xu J Am Chem Soc 2008 130 11828 [20] A R Kortan R Hull R L Opila M G Bawendi M L Steigerwald P J Carroll L E Brus JAm Chem Soc 1990 112 1327 [21] X G Peng M C Schlamp A V Kadavanich A P Alivisatos J Am Chem Soc 1997 119 7019 [22] S Kim B Fisher H J Eisler M Bawendi J Am Chem Soc 2003 125 11466[23] H Zeng J Li Z L Wang J P Liu S Sun Nano Lett 2004 4 187 [24] H Zeng S Sun J Li Z L Wang J P Liu Appl Phys Lett 2004 85 792 [25] Z Xu Y Hou S Sun J Am Chem Soc 2007 129 8698 [26] Y H Lin N Kim J Q Wang C J Zhong J Phys Chem B 2005 109 21593 [27] C Wang H Daimon S Sun Nano Lett 2009 9 1493 [28] K W Kwon M Shim J Am Chem Soc 2005 127 10269 [29] H Yu M Chen P M Rice S X Wang R L White S Sun Nano Lett 2005 5 379 [30] J S Choi Y W Jun S I Yeon H C Kim J S Shin J Cheon J Am Chem Soc 2006 128 15982 [31] S H Choi H B Na Y I Park K An S G Kwon Y Jang M Park J Moon J S Son I CSong W K Moon T Hyeon J Am Chem Soc 2008 130 15573 [32] H W Gu R K Zheng X X Zhang B Xu J Am Chem Soc 2004 126 5664
66
3 Multicomponent Hybrid NPs
Self-assembly processes provide an approach to
fabricating multicomponent hybrid NPs with
integrated multifunctionality
43
- Surfactant polymers
- Sol-gel process
44
Poly(lactico-co-glycolicacid)
(PLGA)
Poly(L-lysine)-PEG-folate
(PLL-PEG-FOL)
By mechanical
stirring at RT
in CH3Cl
45
46
W Lu SR Arumugam D Senapati ACSNANO 2010 4(3) 1739ndash1749
GNP GNP
GNP
RNA Aptamer
Anti-HER2C-reb-2 antibody
GNPCTAB
47
Magnetic nanoparticle surface modification Glucose oxidasse
(GOx)
PEGGPS
APSMNP
48Z Lin et al Analytica Chimica Acta 818 (2014) 54ndash60
49
P Sun H Zhang C Liu Langmuir 2010 26(2) 1278ndash1284 50
Immuno-labeling using Fe3O4CdTe nanocomposites which are formed by
linking multiple TGA-stabilized CdTe QDs with the thiol-functionalized silica
coated iron oxide nanoparticles
23-dimercaptosuccinic
acids (DMSA)Dopamine
Mercaptoundecanoic acid
(MUA)
HS COOH
4 Small multifunctional molecules
Glutaraldehyde 51
C J Xu K M Xu H W Gu R K Zheng H Liu X X Zhang Z H Guo B Xu J Am Chem Soc 2004 126 9938
Dopamine
Conditions (a) NaOH tert-butyl dicarbonate dioxane H2O 24 hrs (b) BnBr K2CO3 DMF
RT 24 h (c) 10 CF3COOH CH2Cl2 rt 5 h (d) succinic anhydride pyridine RTt 3 h (e)
NHS DCC DMAP CHCl3 RTt 3 h
NTA
52
3-mercapto trimethoxysilane
(MPS)
3-amino trimethoxysilane
(APS)
5 Silane multifunctional monomers
Glycidoxypropyltrimethoxy-silane
(GPS)
53
54
Multifunctional magnetic nanoparticles
application
Bioseparation
Biodetection(Real time monitoring)
Targeted drug delivery
Magnetic fluid Hyperthermia therapy
55
1 Specific Bio-Separation
bull Magnetic properties
bull Targeting agent
Cell organism molecule
56
His-tagged protein purification as
the first step towards recombinant
protein purification
58
Lysing Binding
Nickel
affinity
nanoparicle
His tag
fusion protein
Washing Elusion
Digestion
Binding
Nickel
affinity
nanoparticle
Washing Target
Recombinant
Protein
1- Preparation of Fe3O4SiO2Layered Double Hydroxide CoreminusShell
Microspheres for Magnetic Separation of Proteins J Am ChemSoc (2012)
18 134(2) 1071-7
Ni2+
2- Magnetic Nanocomposite Spheres Decorated with NiO Nanoparticles for a
Magnetically Recyclable Protein Separation System J Kim Y Piao N Lee Y
Park I-H Lee J-H Lee Adv Mater (2010) 22 57ndash60
His-tagged protein purification
Purification of His6-lipase using Fe3O4AundashLAndashIDAndashCu2+ NPs with various
amounts of NPs (lane 1 01 mg lane 2 02 mg lane 3
63
Synthetic procedure for the coreshell structure spheres
64
glutaraldehyde
(GLH)
Reference[1] H W Gu Z M Yang J H Gao C K Chang B Xu J Am Chem Soc 2005 127 34
[2] A Hutten D Sudfeld I Ennen G Reiss K Wojczykowski P Jutzi J Magn Magn Mater 2005
293 93
[3] G S Chaubey C Barcena N Poudyal C B Rong J M Gao S Sun J P Liu J Am Chem
Soc 2007 129 7214
[4] C W Kim Y H Kim H G Cha D K Lee Y S Kang J NanosciNanotechnol 2006 6 3417
[5] Y W Jun J S Choi J Cheon Angew Chem Int Ed 2006 45 3414
[6] X Michalet F F Pinaud L A Bentolila J M Tsay S Doose J J Li G Sundaresan A M Wu
S S Gambhir S Weiss Science 2005 307 538
[7] M Hu J Y Chen Z Y Li L Au G V Hartland X D Li M Marquez Y N Xia Chem Soc
Rev 2006 35 1084
[8] M Vallet-Regi F Balas D Arcos Angew Chem Int Ed 2007 46 7548
[9] S T Selvan P K Patra C Y Ang J Y Ying Angew Chem Int Ed 2007 46 2448
[10] I S Lee N Lee J Park B H Kim Y W Yi T Kim T K Kim I H Lee S R Paik T
Hyeon J Am Chem Soc 2006 128 10658
[11] Y S Lin S H Wu Y Hung Y H Chou C Chang M L Lin C P Tsai C Y Mou Chem
Mater 2006 18 5170
[12] B H Wu H Zhang C Chen S C Lin N F Zheng Nano Res 2009 2 975
[13] J Kim J E Lee J Lee J H Yu B C Kim K An Y Hwang C Shin J G Park J Kim T
Hyeon J Am Chem Soc 2006 128 688
[14] S Santra H S Yang P H Holloway J T Stanley R A Mericle J Am Chem Soc 2005 127
1656
[15] D K Yi S T Selvan S S Lee G C Papaefthymiou D Kundaliya J Y Ying J Am Chem
Soc 2005 127 4990 65
[16] J H Gao B Zhang Y Gao Y Pan X X Zhang B Xu J Am Chem Soc 2007 129 11928 [17] J H Gao W Zhang P B Huang B Zhang X X Zhang B Xu J Am Chem Soc 2008 130 3710 [18] J H Gao G L Liang B Zhang Y Kuang X X Zhang B Xu J Am Chem Soc 2007 129 1428 [19] J H Gao G L Liang J S Cheung Y Pan Y Kuang F Zhao B Zhang X X Zhang E X Wu B Xu J Am Chem Soc 2008 130 11828 [20] A R Kortan R Hull R L Opila M G Bawendi M L Steigerwald P J Carroll L E Brus JAm Chem Soc 1990 112 1327 [21] X G Peng M C Schlamp A V Kadavanich A P Alivisatos J Am Chem Soc 1997 119 7019 [22] S Kim B Fisher H J Eisler M Bawendi J Am Chem Soc 2003 125 11466[23] H Zeng J Li Z L Wang J P Liu S Sun Nano Lett 2004 4 187 [24] H Zeng S Sun J Li Z L Wang J P Liu Appl Phys Lett 2004 85 792 [25] Z Xu Y Hou S Sun J Am Chem Soc 2007 129 8698 [26] Y H Lin N Kim J Q Wang C J Zhong J Phys Chem B 2005 109 21593 [27] C Wang H Daimon S Sun Nano Lett 2009 9 1493 [28] K W Kwon M Shim J Am Chem Soc 2005 127 10269 [29] H Yu M Chen P M Rice S X Wang R L White S Sun Nano Lett 2005 5 379 [30] J S Choi Y W Jun S I Yeon H C Kim J S Shin J Cheon J Am Chem Soc 2006 128 15982 [31] S H Choi H B Na Y I Park K An S G Kwon Y Jang M Park J Moon J S Son I CSong W K Moon T Hyeon J Am Chem Soc 2008 130 15573 [32] H W Gu R K Zheng X X Zhang B Xu J Am Chem Soc 2004 126 5664
66
44
Poly(lactico-co-glycolicacid)
(PLGA)
Poly(L-lysine)-PEG-folate
(PLL-PEG-FOL)
By mechanical
stirring at RT
in CH3Cl
45
46
W Lu SR Arumugam D Senapati ACSNANO 2010 4(3) 1739ndash1749
GNP GNP
GNP
RNA Aptamer
Anti-HER2C-reb-2 antibody
GNPCTAB
47
Magnetic nanoparticle surface modification Glucose oxidasse
(GOx)
PEGGPS
APSMNP
48Z Lin et al Analytica Chimica Acta 818 (2014) 54ndash60
49
P Sun H Zhang C Liu Langmuir 2010 26(2) 1278ndash1284 50
Immuno-labeling using Fe3O4CdTe nanocomposites which are formed by
linking multiple TGA-stabilized CdTe QDs with the thiol-functionalized silica
coated iron oxide nanoparticles
23-dimercaptosuccinic
acids (DMSA)Dopamine
Mercaptoundecanoic acid
(MUA)
HS COOH
4 Small multifunctional molecules
Glutaraldehyde 51
C J Xu K M Xu H W Gu R K Zheng H Liu X X Zhang Z H Guo B Xu J Am Chem Soc 2004 126 9938
Dopamine
Conditions (a) NaOH tert-butyl dicarbonate dioxane H2O 24 hrs (b) BnBr K2CO3 DMF
RT 24 h (c) 10 CF3COOH CH2Cl2 rt 5 h (d) succinic anhydride pyridine RTt 3 h (e)
NHS DCC DMAP CHCl3 RTt 3 h
NTA
52
3-mercapto trimethoxysilane
(MPS)
3-amino trimethoxysilane
(APS)
5 Silane multifunctional monomers
Glycidoxypropyltrimethoxy-silane
(GPS)
53
54
Multifunctional magnetic nanoparticles
application
Bioseparation
Biodetection(Real time monitoring)
Targeted drug delivery
Magnetic fluid Hyperthermia therapy
55
1 Specific Bio-Separation
bull Magnetic properties
bull Targeting agent
Cell organism molecule
56
His-tagged protein purification as
the first step towards recombinant
protein purification
58
Lysing Binding
Nickel
affinity
nanoparicle
His tag
fusion protein
Washing Elusion
Digestion
Binding
Nickel
affinity
nanoparticle
Washing Target
Recombinant
Protein
1- Preparation of Fe3O4SiO2Layered Double Hydroxide CoreminusShell
Microspheres for Magnetic Separation of Proteins J Am ChemSoc (2012)
18 134(2) 1071-7
Ni2+
2- Magnetic Nanocomposite Spheres Decorated with NiO Nanoparticles for a
Magnetically Recyclable Protein Separation System J Kim Y Piao N Lee Y
Park I-H Lee J-H Lee Adv Mater (2010) 22 57ndash60
His-tagged protein purification
Purification of His6-lipase using Fe3O4AundashLAndashIDAndashCu2+ NPs with various
amounts of NPs (lane 1 01 mg lane 2 02 mg lane 3
63
Synthetic procedure for the coreshell structure spheres
64
glutaraldehyde
(GLH)
Reference[1] H W Gu Z M Yang J H Gao C K Chang B Xu J Am Chem Soc 2005 127 34
[2] A Hutten D Sudfeld I Ennen G Reiss K Wojczykowski P Jutzi J Magn Magn Mater 2005
293 93
[3] G S Chaubey C Barcena N Poudyal C B Rong J M Gao S Sun J P Liu J Am Chem
Soc 2007 129 7214
[4] C W Kim Y H Kim H G Cha D K Lee Y S Kang J NanosciNanotechnol 2006 6 3417
[5] Y W Jun J S Choi J Cheon Angew Chem Int Ed 2006 45 3414
[6] X Michalet F F Pinaud L A Bentolila J M Tsay S Doose J J Li G Sundaresan A M Wu
S S Gambhir S Weiss Science 2005 307 538
[7] M Hu J Y Chen Z Y Li L Au G V Hartland X D Li M Marquez Y N Xia Chem Soc
Rev 2006 35 1084
[8] M Vallet-Regi F Balas D Arcos Angew Chem Int Ed 2007 46 7548
[9] S T Selvan P K Patra C Y Ang J Y Ying Angew Chem Int Ed 2007 46 2448
[10] I S Lee N Lee J Park B H Kim Y W Yi T Kim T K Kim I H Lee S R Paik T
Hyeon J Am Chem Soc 2006 128 10658
[11] Y S Lin S H Wu Y Hung Y H Chou C Chang M L Lin C P Tsai C Y Mou Chem
Mater 2006 18 5170
[12] B H Wu H Zhang C Chen S C Lin N F Zheng Nano Res 2009 2 975
[13] J Kim J E Lee J Lee J H Yu B C Kim K An Y Hwang C Shin J G Park J Kim T
Hyeon J Am Chem Soc 2006 128 688
[14] S Santra H S Yang P H Holloway J T Stanley R A Mericle J Am Chem Soc 2005 127
1656
[15] D K Yi S T Selvan S S Lee G C Papaefthymiou D Kundaliya J Y Ying J Am Chem
Soc 2005 127 4990 65
[16] J H Gao B Zhang Y Gao Y Pan X X Zhang B Xu J Am Chem Soc 2007 129 11928 [17] J H Gao W Zhang P B Huang B Zhang X X Zhang B Xu J Am Chem Soc 2008 130 3710 [18] J H Gao G L Liang B Zhang Y Kuang X X Zhang B Xu J Am Chem Soc 2007 129 1428 [19] J H Gao G L Liang J S Cheung Y Pan Y Kuang F Zhao B Zhang X X Zhang E X Wu B Xu J Am Chem Soc 2008 130 11828 [20] A R Kortan R Hull R L Opila M G Bawendi M L Steigerwald P J Carroll L E Brus JAm Chem Soc 1990 112 1327 [21] X G Peng M C Schlamp A V Kadavanich A P Alivisatos J Am Chem Soc 1997 119 7019 [22] S Kim B Fisher H J Eisler M Bawendi J Am Chem Soc 2003 125 11466[23] H Zeng J Li Z L Wang J P Liu S Sun Nano Lett 2004 4 187 [24] H Zeng S Sun J Li Z L Wang J P Liu Appl Phys Lett 2004 85 792 [25] Z Xu Y Hou S Sun J Am Chem Soc 2007 129 8698 [26] Y H Lin N Kim J Q Wang C J Zhong J Phys Chem B 2005 109 21593 [27] C Wang H Daimon S Sun Nano Lett 2009 9 1493 [28] K W Kwon M Shim J Am Chem Soc 2005 127 10269 [29] H Yu M Chen P M Rice S X Wang R L White S Sun Nano Lett 2005 5 379 [30] J S Choi Y W Jun S I Yeon H C Kim J S Shin J Cheon J Am Chem Soc 2006 128 15982 [31] S H Choi H B Na Y I Park K An S G Kwon Y Jang M Park J Moon J S Son I CSong W K Moon T Hyeon J Am Chem Soc 2008 130 15573 [32] H W Gu R K Zheng X X Zhang B Xu J Am Chem Soc 2004 126 5664
66
45
46
W Lu SR Arumugam D Senapati ACSNANO 2010 4(3) 1739ndash1749
GNP GNP
GNP
RNA Aptamer
Anti-HER2C-reb-2 antibody
GNPCTAB
47
Magnetic nanoparticle surface modification Glucose oxidasse
(GOx)
PEGGPS
APSMNP
48Z Lin et al Analytica Chimica Acta 818 (2014) 54ndash60
49
P Sun H Zhang C Liu Langmuir 2010 26(2) 1278ndash1284 50
Immuno-labeling using Fe3O4CdTe nanocomposites which are formed by
linking multiple TGA-stabilized CdTe QDs with the thiol-functionalized silica
coated iron oxide nanoparticles
23-dimercaptosuccinic
acids (DMSA)Dopamine
Mercaptoundecanoic acid
(MUA)
HS COOH
4 Small multifunctional molecules
Glutaraldehyde 51
C J Xu K M Xu H W Gu R K Zheng H Liu X X Zhang Z H Guo B Xu J Am Chem Soc 2004 126 9938
Dopamine
Conditions (a) NaOH tert-butyl dicarbonate dioxane H2O 24 hrs (b) BnBr K2CO3 DMF
RT 24 h (c) 10 CF3COOH CH2Cl2 rt 5 h (d) succinic anhydride pyridine RTt 3 h (e)
NHS DCC DMAP CHCl3 RTt 3 h
NTA
52
3-mercapto trimethoxysilane
(MPS)
3-amino trimethoxysilane
(APS)
5 Silane multifunctional monomers
Glycidoxypropyltrimethoxy-silane
(GPS)
53
54
Multifunctional magnetic nanoparticles
application
Bioseparation
Biodetection(Real time monitoring)
Targeted drug delivery
Magnetic fluid Hyperthermia therapy
55
1 Specific Bio-Separation
bull Magnetic properties
bull Targeting agent
Cell organism molecule
56
His-tagged protein purification as
the first step towards recombinant
protein purification
58
Lysing Binding
Nickel
affinity
nanoparicle
His tag
fusion protein
Washing Elusion
Digestion
Binding
Nickel
affinity
nanoparticle
Washing Target
Recombinant
Protein
1- Preparation of Fe3O4SiO2Layered Double Hydroxide CoreminusShell
Microspheres for Magnetic Separation of Proteins J Am ChemSoc (2012)
18 134(2) 1071-7
Ni2+
2- Magnetic Nanocomposite Spheres Decorated with NiO Nanoparticles for a
Magnetically Recyclable Protein Separation System J Kim Y Piao N Lee Y
Park I-H Lee J-H Lee Adv Mater (2010) 22 57ndash60
His-tagged protein purification
Purification of His6-lipase using Fe3O4AundashLAndashIDAndashCu2+ NPs with various
amounts of NPs (lane 1 01 mg lane 2 02 mg lane 3
63
Synthetic procedure for the coreshell structure spheres
64
glutaraldehyde
(GLH)
Reference[1] H W Gu Z M Yang J H Gao C K Chang B Xu J Am Chem Soc 2005 127 34
[2] A Hutten D Sudfeld I Ennen G Reiss K Wojczykowski P Jutzi J Magn Magn Mater 2005
293 93
[3] G S Chaubey C Barcena N Poudyal C B Rong J M Gao S Sun J P Liu J Am Chem
Soc 2007 129 7214
[4] C W Kim Y H Kim H G Cha D K Lee Y S Kang J NanosciNanotechnol 2006 6 3417
[5] Y W Jun J S Choi J Cheon Angew Chem Int Ed 2006 45 3414
[6] X Michalet F F Pinaud L A Bentolila J M Tsay S Doose J J Li G Sundaresan A M Wu
S S Gambhir S Weiss Science 2005 307 538
[7] M Hu J Y Chen Z Y Li L Au G V Hartland X D Li M Marquez Y N Xia Chem Soc
Rev 2006 35 1084
[8] M Vallet-Regi F Balas D Arcos Angew Chem Int Ed 2007 46 7548
[9] S T Selvan P K Patra C Y Ang J Y Ying Angew Chem Int Ed 2007 46 2448
[10] I S Lee N Lee J Park B H Kim Y W Yi T Kim T K Kim I H Lee S R Paik T
Hyeon J Am Chem Soc 2006 128 10658
[11] Y S Lin S H Wu Y Hung Y H Chou C Chang M L Lin C P Tsai C Y Mou Chem
Mater 2006 18 5170
[12] B H Wu H Zhang C Chen S C Lin N F Zheng Nano Res 2009 2 975
[13] J Kim J E Lee J Lee J H Yu B C Kim K An Y Hwang C Shin J G Park J Kim T
Hyeon J Am Chem Soc 2006 128 688
[14] S Santra H S Yang P H Holloway J T Stanley R A Mericle J Am Chem Soc 2005 127
1656
[15] D K Yi S T Selvan S S Lee G C Papaefthymiou D Kundaliya J Y Ying J Am Chem
Soc 2005 127 4990 65
[16] J H Gao B Zhang Y Gao Y Pan X X Zhang B Xu J Am Chem Soc 2007 129 11928 [17] J H Gao W Zhang P B Huang B Zhang X X Zhang B Xu J Am Chem Soc 2008 130 3710 [18] J H Gao G L Liang B Zhang Y Kuang X X Zhang B Xu J Am Chem Soc 2007 129 1428 [19] J H Gao G L Liang J S Cheung Y Pan Y Kuang F Zhao B Zhang X X Zhang E X Wu B Xu J Am Chem Soc 2008 130 11828 [20] A R Kortan R Hull R L Opila M G Bawendi M L Steigerwald P J Carroll L E Brus JAm Chem Soc 1990 112 1327 [21] X G Peng M C Schlamp A V Kadavanich A P Alivisatos J Am Chem Soc 1997 119 7019 [22] S Kim B Fisher H J Eisler M Bawendi J Am Chem Soc 2003 125 11466[23] H Zeng J Li Z L Wang J P Liu S Sun Nano Lett 2004 4 187 [24] H Zeng S Sun J Li Z L Wang J P Liu Appl Phys Lett 2004 85 792 [25] Z Xu Y Hou S Sun J Am Chem Soc 2007 129 8698 [26] Y H Lin N Kim J Q Wang C J Zhong J Phys Chem B 2005 109 21593 [27] C Wang H Daimon S Sun Nano Lett 2009 9 1493 [28] K W Kwon M Shim J Am Chem Soc 2005 127 10269 [29] H Yu M Chen P M Rice S X Wang R L White S Sun Nano Lett 2005 5 379 [30] J S Choi Y W Jun S I Yeon H C Kim J S Shin J Cheon J Am Chem Soc 2006 128 15982 [31] S H Choi H B Na Y I Park K An S G Kwon Y Jang M Park J Moon J S Son I CSong W K Moon T Hyeon J Am Chem Soc 2008 130 15573 [32] H W Gu R K Zheng X X Zhang B Xu J Am Chem Soc 2004 126 5664
66
46
W Lu SR Arumugam D Senapati ACSNANO 2010 4(3) 1739ndash1749
GNP GNP
GNP
RNA Aptamer
Anti-HER2C-reb-2 antibody
GNPCTAB
47
Magnetic nanoparticle surface modification Glucose oxidasse
(GOx)
PEGGPS
APSMNP
48Z Lin et al Analytica Chimica Acta 818 (2014) 54ndash60
49
P Sun H Zhang C Liu Langmuir 2010 26(2) 1278ndash1284 50
Immuno-labeling using Fe3O4CdTe nanocomposites which are formed by
linking multiple TGA-stabilized CdTe QDs with the thiol-functionalized silica
coated iron oxide nanoparticles
23-dimercaptosuccinic
acids (DMSA)Dopamine
Mercaptoundecanoic acid
(MUA)
HS COOH
4 Small multifunctional molecules
Glutaraldehyde 51
C J Xu K M Xu H W Gu R K Zheng H Liu X X Zhang Z H Guo B Xu J Am Chem Soc 2004 126 9938
Dopamine
Conditions (a) NaOH tert-butyl dicarbonate dioxane H2O 24 hrs (b) BnBr K2CO3 DMF
RT 24 h (c) 10 CF3COOH CH2Cl2 rt 5 h (d) succinic anhydride pyridine RTt 3 h (e)
NHS DCC DMAP CHCl3 RTt 3 h
NTA
52
3-mercapto trimethoxysilane
(MPS)
3-amino trimethoxysilane
(APS)
5 Silane multifunctional monomers
Glycidoxypropyltrimethoxy-silane
(GPS)
53
54
Multifunctional magnetic nanoparticles
application
Bioseparation
Biodetection(Real time monitoring)
Targeted drug delivery
Magnetic fluid Hyperthermia therapy
55
1 Specific Bio-Separation
bull Magnetic properties
bull Targeting agent
Cell organism molecule
56
His-tagged protein purification as
the first step towards recombinant
protein purification
58
Lysing Binding
Nickel
affinity
nanoparicle
His tag
fusion protein
Washing Elusion
Digestion
Binding
Nickel
affinity
nanoparticle
Washing Target
Recombinant
Protein
1- Preparation of Fe3O4SiO2Layered Double Hydroxide CoreminusShell
Microspheres for Magnetic Separation of Proteins J Am ChemSoc (2012)
18 134(2) 1071-7
Ni2+
2- Magnetic Nanocomposite Spheres Decorated with NiO Nanoparticles for a
Magnetically Recyclable Protein Separation System J Kim Y Piao N Lee Y
Park I-H Lee J-H Lee Adv Mater (2010) 22 57ndash60
His-tagged protein purification
Purification of His6-lipase using Fe3O4AundashLAndashIDAndashCu2+ NPs with various
amounts of NPs (lane 1 01 mg lane 2 02 mg lane 3
63
Synthetic procedure for the coreshell structure spheres
64
glutaraldehyde
(GLH)
Reference[1] H W Gu Z M Yang J H Gao C K Chang B Xu J Am Chem Soc 2005 127 34
[2] A Hutten D Sudfeld I Ennen G Reiss K Wojczykowski P Jutzi J Magn Magn Mater 2005
293 93
[3] G S Chaubey C Barcena N Poudyal C B Rong J M Gao S Sun J P Liu J Am Chem
Soc 2007 129 7214
[4] C W Kim Y H Kim H G Cha D K Lee Y S Kang J NanosciNanotechnol 2006 6 3417
[5] Y W Jun J S Choi J Cheon Angew Chem Int Ed 2006 45 3414
[6] X Michalet F F Pinaud L A Bentolila J M Tsay S Doose J J Li G Sundaresan A M Wu
S S Gambhir S Weiss Science 2005 307 538
[7] M Hu J Y Chen Z Y Li L Au G V Hartland X D Li M Marquez Y N Xia Chem Soc
Rev 2006 35 1084
[8] M Vallet-Regi F Balas D Arcos Angew Chem Int Ed 2007 46 7548
[9] S T Selvan P K Patra C Y Ang J Y Ying Angew Chem Int Ed 2007 46 2448
[10] I S Lee N Lee J Park B H Kim Y W Yi T Kim T K Kim I H Lee S R Paik T
Hyeon J Am Chem Soc 2006 128 10658
[11] Y S Lin S H Wu Y Hung Y H Chou C Chang M L Lin C P Tsai C Y Mou Chem
Mater 2006 18 5170
[12] B H Wu H Zhang C Chen S C Lin N F Zheng Nano Res 2009 2 975
[13] J Kim J E Lee J Lee J H Yu B C Kim K An Y Hwang C Shin J G Park J Kim T
Hyeon J Am Chem Soc 2006 128 688
[14] S Santra H S Yang P H Holloway J T Stanley R A Mericle J Am Chem Soc 2005 127
1656
[15] D K Yi S T Selvan S S Lee G C Papaefthymiou D Kundaliya J Y Ying J Am Chem
Soc 2005 127 4990 65
[16] J H Gao B Zhang Y Gao Y Pan X X Zhang B Xu J Am Chem Soc 2007 129 11928 [17] J H Gao W Zhang P B Huang B Zhang X X Zhang B Xu J Am Chem Soc 2008 130 3710 [18] J H Gao G L Liang B Zhang Y Kuang X X Zhang B Xu J Am Chem Soc 2007 129 1428 [19] J H Gao G L Liang J S Cheung Y Pan Y Kuang F Zhao B Zhang X X Zhang E X Wu B Xu J Am Chem Soc 2008 130 11828 [20] A R Kortan R Hull R L Opila M G Bawendi M L Steigerwald P J Carroll L E Brus JAm Chem Soc 1990 112 1327 [21] X G Peng M C Schlamp A V Kadavanich A P Alivisatos J Am Chem Soc 1997 119 7019 [22] S Kim B Fisher H J Eisler M Bawendi J Am Chem Soc 2003 125 11466[23] H Zeng J Li Z L Wang J P Liu S Sun Nano Lett 2004 4 187 [24] H Zeng S Sun J Li Z L Wang J P Liu Appl Phys Lett 2004 85 792 [25] Z Xu Y Hou S Sun J Am Chem Soc 2007 129 8698 [26] Y H Lin N Kim J Q Wang C J Zhong J Phys Chem B 2005 109 21593 [27] C Wang H Daimon S Sun Nano Lett 2009 9 1493 [28] K W Kwon M Shim J Am Chem Soc 2005 127 10269 [29] H Yu M Chen P M Rice S X Wang R L White S Sun Nano Lett 2005 5 379 [30] J S Choi Y W Jun S I Yeon H C Kim J S Shin J Cheon J Am Chem Soc 2006 128 15982 [31] S H Choi H B Na Y I Park K An S G Kwon Y Jang M Park J Moon J S Son I CSong W K Moon T Hyeon J Am Chem Soc 2008 130 15573 [32] H W Gu R K Zheng X X Zhang B Xu J Am Chem Soc 2004 126 5664
66
47
Magnetic nanoparticle surface modification Glucose oxidasse
(GOx)
PEGGPS
APSMNP
48Z Lin et al Analytica Chimica Acta 818 (2014) 54ndash60
49
P Sun H Zhang C Liu Langmuir 2010 26(2) 1278ndash1284 50
Immuno-labeling using Fe3O4CdTe nanocomposites which are formed by
linking multiple TGA-stabilized CdTe QDs with the thiol-functionalized silica
coated iron oxide nanoparticles
23-dimercaptosuccinic
acids (DMSA)Dopamine
Mercaptoundecanoic acid
(MUA)
HS COOH
4 Small multifunctional molecules
Glutaraldehyde 51
C J Xu K M Xu H W Gu R K Zheng H Liu X X Zhang Z H Guo B Xu J Am Chem Soc 2004 126 9938
Dopamine
Conditions (a) NaOH tert-butyl dicarbonate dioxane H2O 24 hrs (b) BnBr K2CO3 DMF
RT 24 h (c) 10 CF3COOH CH2Cl2 rt 5 h (d) succinic anhydride pyridine RTt 3 h (e)
NHS DCC DMAP CHCl3 RTt 3 h
NTA
52
3-mercapto trimethoxysilane
(MPS)
3-amino trimethoxysilane
(APS)
5 Silane multifunctional monomers
Glycidoxypropyltrimethoxy-silane
(GPS)
53
54
Multifunctional magnetic nanoparticles
application
Bioseparation
Biodetection(Real time monitoring)
Targeted drug delivery
Magnetic fluid Hyperthermia therapy
55
1 Specific Bio-Separation
bull Magnetic properties
bull Targeting agent
Cell organism molecule
56
His-tagged protein purification as
the first step towards recombinant
protein purification
58
Lysing Binding
Nickel
affinity
nanoparicle
His tag
fusion protein
Washing Elusion
Digestion
Binding
Nickel
affinity
nanoparticle
Washing Target
Recombinant
Protein
1- Preparation of Fe3O4SiO2Layered Double Hydroxide CoreminusShell
Microspheres for Magnetic Separation of Proteins J Am ChemSoc (2012)
18 134(2) 1071-7
Ni2+
2- Magnetic Nanocomposite Spheres Decorated with NiO Nanoparticles for a
Magnetically Recyclable Protein Separation System J Kim Y Piao N Lee Y
Park I-H Lee J-H Lee Adv Mater (2010) 22 57ndash60
His-tagged protein purification
Purification of His6-lipase using Fe3O4AundashLAndashIDAndashCu2+ NPs with various
amounts of NPs (lane 1 01 mg lane 2 02 mg lane 3
63
Synthetic procedure for the coreshell structure spheres
64
glutaraldehyde
(GLH)
Reference[1] H W Gu Z M Yang J H Gao C K Chang B Xu J Am Chem Soc 2005 127 34
[2] A Hutten D Sudfeld I Ennen G Reiss K Wojczykowski P Jutzi J Magn Magn Mater 2005
293 93
[3] G S Chaubey C Barcena N Poudyal C B Rong J M Gao S Sun J P Liu J Am Chem
Soc 2007 129 7214
[4] C W Kim Y H Kim H G Cha D K Lee Y S Kang J NanosciNanotechnol 2006 6 3417
[5] Y W Jun J S Choi J Cheon Angew Chem Int Ed 2006 45 3414
[6] X Michalet F F Pinaud L A Bentolila J M Tsay S Doose J J Li G Sundaresan A M Wu
S S Gambhir S Weiss Science 2005 307 538
[7] M Hu J Y Chen Z Y Li L Au G V Hartland X D Li M Marquez Y N Xia Chem Soc
Rev 2006 35 1084
[8] M Vallet-Regi F Balas D Arcos Angew Chem Int Ed 2007 46 7548
[9] S T Selvan P K Patra C Y Ang J Y Ying Angew Chem Int Ed 2007 46 2448
[10] I S Lee N Lee J Park B H Kim Y W Yi T Kim T K Kim I H Lee S R Paik T
Hyeon J Am Chem Soc 2006 128 10658
[11] Y S Lin S H Wu Y Hung Y H Chou C Chang M L Lin C P Tsai C Y Mou Chem
Mater 2006 18 5170
[12] B H Wu H Zhang C Chen S C Lin N F Zheng Nano Res 2009 2 975
[13] J Kim J E Lee J Lee J H Yu B C Kim K An Y Hwang C Shin J G Park J Kim T
Hyeon J Am Chem Soc 2006 128 688
[14] S Santra H S Yang P H Holloway J T Stanley R A Mericle J Am Chem Soc 2005 127
1656
[15] D K Yi S T Selvan S S Lee G C Papaefthymiou D Kundaliya J Y Ying J Am Chem
Soc 2005 127 4990 65
[16] J H Gao B Zhang Y Gao Y Pan X X Zhang B Xu J Am Chem Soc 2007 129 11928 [17] J H Gao W Zhang P B Huang B Zhang X X Zhang B Xu J Am Chem Soc 2008 130 3710 [18] J H Gao G L Liang B Zhang Y Kuang X X Zhang B Xu J Am Chem Soc 2007 129 1428 [19] J H Gao G L Liang J S Cheung Y Pan Y Kuang F Zhao B Zhang X X Zhang E X Wu B Xu J Am Chem Soc 2008 130 11828 [20] A R Kortan R Hull R L Opila M G Bawendi M L Steigerwald P J Carroll L E Brus JAm Chem Soc 1990 112 1327 [21] X G Peng M C Schlamp A V Kadavanich A P Alivisatos J Am Chem Soc 1997 119 7019 [22] S Kim B Fisher H J Eisler M Bawendi J Am Chem Soc 2003 125 11466[23] H Zeng J Li Z L Wang J P Liu S Sun Nano Lett 2004 4 187 [24] H Zeng S Sun J Li Z L Wang J P Liu Appl Phys Lett 2004 85 792 [25] Z Xu Y Hou S Sun J Am Chem Soc 2007 129 8698 [26] Y H Lin N Kim J Q Wang C J Zhong J Phys Chem B 2005 109 21593 [27] C Wang H Daimon S Sun Nano Lett 2009 9 1493 [28] K W Kwon M Shim J Am Chem Soc 2005 127 10269 [29] H Yu M Chen P M Rice S X Wang R L White S Sun Nano Lett 2005 5 379 [30] J S Choi Y W Jun S I Yeon H C Kim J S Shin J Cheon J Am Chem Soc 2006 128 15982 [31] S H Choi H B Na Y I Park K An S G Kwon Y Jang M Park J Moon J S Son I CSong W K Moon T Hyeon J Am Chem Soc 2008 130 15573 [32] H W Gu R K Zheng X X Zhang B Xu J Am Chem Soc 2004 126 5664
66
48Z Lin et al Analytica Chimica Acta 818 (2014) 54ndash60
49
P Sun H Zhang C Liu Langmuir 2010 26(2) 1278ndash1284 50
Immuno-labeling using Fe3O4CdTe nanocomposites which are formed by
linking multiple TGA-stabilized CdTe QDs with the thiol-functionalized silica
coated iron oxide nanoparticles
23-dimercaptosuccinic
acids (DMSA)Dopamine
Mercaptoundecanoic acid
(MUA)
HS COOH
4 Small multifunctional molecules
Glutaraldehyde 51
C J Xu K M Xu H W Gu R K Zheng H Liu X X Zhang Z H Guo B Xu J Am Chem Soc 2004 126 9938
Dopamine
Conditions (a) NaOH tert-butyl dicarbonate dioxane H2O 24 hrs (b) BnBr K2CO3 DMF
RT 24 h (c) 10 CF3COOH CH2Cl2 rt 5 h (d) succinic anhydride pyridine RTt 3 h (e)
NHS DCC DMAP CHCl3 RTt 3 h
NTA
52
3-mercapto trimethoxysilane
(MPS)
3-amino trimethoxysilane
(APS)
5 Silane multifunctional monomers
Glycidoxypropyltrimethoxy-silane
(GPS)
53
54
Multifunctional magnetic nanoparticles
application
Bioseparation
Biodetection(Real time monitoring)
Targeted drug delivery
Magnetic fluid Hyperthermia therapy
55
1 Specific Bio-Separation
bull Magnetic properties
bull Targeting agent
Cell organism molecule
56
His-tagged protein purification as
the first step towards recombinant
protein purification
58
Lysing Binding
Nickel
affinity
nanoparicle
His tag
fusion protein
Washing Elusion
Digestion
Binding
Nickel
affinity
nanoparticle
Washing Target
Recombinant
Protein
1- Preparation of Fe3O4SiO2Layered Double Hydroxide CoreminusShell
Microspheres for Magnetic Separation of Proteins J Am ChemSoc (2012)
18 134(2) 1071-7
Ni2+
2- Magnetic Nanocomposite Spheres Decorated with NiO Nanoparticles for a
Magnetically Recyclable Protein Separation System J Kim Y Piao N Lee Y
Park I-H Lee J-H Lee Adv Mater (2010) 22 57ndash60
His-tagged protein purification
Purification of His6-lipase using Fe3O4AundashLAndashIDAndashCu2+ NPs with various
amounts of NPs (lane 1 01 mg lane 2 02 mg lane 3
63
Synthetic procedure for the coreshell structure spheres
64
glutaraldehyde
(GLH)
Reference[1] H W Gu Z M Yang J H Gao C K Chang B Xu J Am Chem Soc 2005 127 34
[2] A Hutten D Sudfeld I Ennen G Reiss K Wojczykowski P Jutzi J Magn Magn Mater 2005
293 93
[3] G S Chaubey C Barcena N Poudyal C B Rong J M Gao S Sun J P Liu J Am Chem
Soc 2007 129 7214
[4] C W Kim Y H Kim H G Cha D K Lee Y S Kang J NanosciNanotechnol 2006 6 3417
[5] Y W Jun J S Choi J Cheon Angew Chem Int Ed 2006 45 3414
[6] X Michalet F F Pinaud L A Bentolila J M Tsay S Doose J J Li G Sundaresan A M Wu
S S Gambhir S Weiss Science 2005 307 538
[7] M Hu J Y Chen Z Y Li L Au G V Hartland X D Li M Marquez Y N Xia Chem Soc
Rev 2006 35 1084
[8] M Vallet-Regi F Balas D Arcos Angew Chem Int Ed 2007 46 7548
[9] S T Selvan P K Patra C Y Ang J Y Ying Angew Chem Int Ed 2007 46 2448
[10] I S Lee N Lee J Park B H Kim Y W Yi T Kim T K Kim I H Lee S R Paik T
Hyeon J Am Chem Soc 2006 128 10658
[11] Y S Lin S H Wu Y Hung Y H Chou C Chang M L Lin C P Tsai C Y Mou Chem
Mater 2006 18 5170
[12] B H Wu H Zhang C Chen S C Lin N F Zheng Nano Res 2009 2 975
[13] J Kim J E Lee J Lee J H Yu B C Kim K An Y Hwang C Shin J G Park J Kim T
Hyeon J Am Chem Soc 2006 128 688
[14] S Santra H S Yang P H Holloway J T Stanley R A Mericle J Am Chem Soc 2005 127
1656
[15] D K Yi S T Selvan S S Lee G C Papaefthymiou D Kundaliya J Y Ying J Am Chem
Soc 2005 127 4990 65
[16] J H Gao B Zhang Y Gao Y Pan X X Zhang B Xu J Am Chem Soc 2007 129 11928 [17] J H Gao W Zhang P B Huang B Zhang X X Zhang B Xu J Am Chem Soc 2008 130 3710 [18] J H Gao G L Liang B Zhang Y Kuang X X Zhang B Xu J Am Chem Soc 2007 129 1428 [19] J H Gao G L Liang J S Cheung Y Pan Y Kuang F Zhao B Zhang X X Zhang E X Wu B Xu J Am Chem Soc 2008 130 11828 [20] A R Kortan R Hull R L Opila M G Bawendi M L Steigerwald P J Carroll L E Brus JAm Chem Soc 1990 112 1327 [21] X G Peng M C Schlamp A V Kadavanich A P Alivisatos J Am Chem Soc 1997 119 7019 [22] S Kim B Fisher H J Eisler M Bawendi J Am Chem Soc 2003 125 11466[23] H Zeng J Li Z L Wang J P Liu S Sun Nano Lett 2004 4 187 [24] H Zeng S Sun J Li Z L Wang J P Liu Appl Phys Lett 2004 85 792 [25] Z Xu Y Hou S Sun J Am Chem Soc 2007 129 8698 [26] Y H Lin N Kim J Q Wang C J Zhong J Phys Chem B 2005 109 21593 [27] C Wang H Daimon S Sun Nano Lett 2009 9 1493 [28] K W Kwon M Shim J Am Chem Soc 2005 127 10269 [29] H Yu M Chen P M Rice S X Wang R L White S Sun Nano Lett 2005 5 379 [30] J S Choi Y W Jun S I Yeon H C Kim J S Shin J Cheon J Am Chem Soc 2006 128 15982 [31] S H Choi H B Na Y I Park K An S G Kwon Y Jang M Park J Moon J S Son I CSong W K Moon T Hyeon J Am Chem Soc 2008 130 15573 [32] H W Gu R K Zheng X X Zhang B Xu J Am Chem Soc 2004 126 5664
66
49
P Sun H Zhang C Liu Langmuir 2010 26(2) 1278ndash1284 50
Immuno-labeling using Fe3O4CdTe nanocomposites which are formed by
linking multiple TGA-stabilized CdTe QDs with the thiol-functionalized silica
coated iron oxide nanoparticles
23-dimercaptosuccinic
acids (DMSA)Dopamine
Mercaptoundecanoic acid
(MUA)
HS COOH
4 Small multifunctional molecules
Glutaraldehyde 51
C J Xu K M Xu H W Gu R K Zheng H Liu X X Zhang Z H Guo B Xu J Am Chem Soc 2004 126 9938
Dopamine
Conditions (a) NaOH tert-butyl dicarbonate dioxane H2O 24 hrs (b) BnBr K2CO3 DMF
RT 24 h (c) 10 CF3COOH CH2Cl2 rt 5 h (d) succinic anhydride pyridine RTt 3 h (e)
NHS DCC DMAP CHCl3 RTt 3 h
NTA
52
3-mercapto trimethoxysilane
(MPS)
3-amino trimethoxysilane
(APS)
5 Silane multifunctional monomers
Glycidoxypropyltrimethoxy-silane
(GPS)
53
54
Multifunctional magnetic nanoparticles
application
Bioseparation
Biodetection(Real time monitoring)
Targeted drug delivery
Magnetic fluid Hyperthermia therapy
55
1 Specific Bio-Separation
bull Magnetic properties
bull Targeting agent
Cell organism molecule
56
His-tagged protein purification as
the first step towards recombinant
protein purification
58
Lysing Binding
Nickel
affinity
nanoparicle
His tag
fusion protein
Washing Elusion
Digestion
Binding
Nickel
affinity
nanoparticle
Washing Target
Recombinant
Protein
1- Preparation of Fe3O4SiO2Layered Double Hydroxide CoreminusShell
Microspheres for Magnetic Separation of Proteins J Am ChemSoc (2012)
18 134(2) 1071-7
Ni2+
2- Magnetic Nanocomposite Spheres Decorated with NiO Nanoparticles for a
Magnetically Recyclable Protein Separation System J Kim Y Piao N Lee Y
Park I-H Lee J-H Lee Adv Mater (2010) 22 57ndash60
His-tagged protein purification
Purification of His6-lipase using Fe3O4AundashLAndashIDAndashCu2+ NPs with various
amounts of NPs (lane 1 01 mg lane 2 02 mg lane 3
63
Synthetic procedure for the coreshell structure spheres
64
glutaraldehyde
(GLH)
Reference[1] H W Gu Z M Yang J H Gao C K Chang B Xu J Am Chem Soc 2005 127 34
[2] A Hutten D Sudfeld I Ennen G Reiss K Wojczykowski P Jutzi J Magn Magn Mater 2005
293 93
[3] G S Chaubey C Barcena N Poudyal C B Rong J M Gao S Sun J P Liu J Am Chem
Soc 2007 129 7214
[4] C W Kim Y H Kim H G Cha D K Lee Y S Kang J NanosciNanotechnol 2006 6 3417
[5] Y W Jun J S Choi J Cheon Angew Chem Int Ed 2006 45 3414
[6] X Michalet F F Pinaud L A Bentolila J M Tsay S Doose J J Li G Sundaresan A M Wu
S S Gambhir S Weiss Science 2005 307 538
[7] M Hu J Y Chen Z Y Li L Au G V Hartland X D Li M Marquez Y N Xia Chem Soc
Rev 2006 35 1084
[8] M Vallet-Regi F Balas D Arcos Angew Chem Int Ed 2007 46 7548
[9] S T Selvan P K Patra C Y Ang J Y Ying Angew Chem Int Ed 2007 46 2448
[10] I S Lee N Lee J Park B H Kim Y W Yi T Kim T K Kim I H Lee S R Paik T
Hyeon J Am Chem Soc 2006 128 10658
[11] Y S Lin S H Wu Y Hung Y H Chou C Chang M L Lin C P Tsai C Y Mou Chem
Mater 2006 18 5170
[12] B H Wu H Zhang C Chen S C Lin N F Zheng Nano Res 2009 2 975
[13] J Kim J E Lee J Lee J H Yu B C Kim K An Y Hwang C Shin J G Park J Kim T
Hyeon J Am Chem Soc 2006 128 688
[14] S Santra H S Yang P H Holloway J T Stanley R A Mericle J Am Chem Soc 2005 127
1656
[15] D K Yi S T Selvan S S Lee G C Papaefthymiou D Kundaliya J Y Ying J Am Chem
Soc 2005 127 4990 65
[16] J H Gao B Zhang Y Gao Y Pan X X Zhang B Xu J Am Chem Soc 2007 129 11928 [17] J H Gao W Zhang P B Huang B Zhang X X Zhang B Xu J Am Chem Soc 2008 130 3710 [18] J H Gao G L Liang B Zhang Y Kuang X X Zhang B Xu J Am Chem Soc 2007 129 1428 [19] J H Gao G L Liang J S Cheung Y Pan Y Kuang F Zhao B Zhang X X Zhang E X Wu B Xu J Am Chem Soc 2008 130 11828 [20] A R Kortan R Hull R L Opila M G Bawendi M L Steigerwald P J Carroll L E Brus JAm Chem Soc 1990 112 1327 [21] X G Peng M C Schlamp A V Kadavanich A P Alivisatos J Am Chem Soc 1997 119 7019 [22] S Kim B Fisher H J Eisler M Bawendi J Am Chem Soc 2003 125 11466[23] H Zeng J Li Z L Wang J P Liu S Sun Nano Lett 2004 4 187 [24] H Zeng S Sun J Li Z L Wang J P Liu Appl Phys Lett 2004 85 792 [25] Z Xu Y Hou S Sun J Am Chem Soc 2007 129 8698 [26] Y H Lin N Kim J Q Wang C J Zhong J Phys Chem B 2005 109 21593 [27] C Wang H Daimon S Sun Nano Lett 2009 9 1493 [28] K W Kwon M Shim J Am Chem Soc 2005 127 10269 [29] H Yu M Chen P M Rice S X Wang R L White S Sun Nano Lett 2005 5 379 [30] J S Choi Y W Jun S I Yeon H C Kim J S Shin J Cheon J Am Chem Soc 2006 128 15982 [31] S H Choi H B Na Y I Park K An S G Kwon Y Jang M Park J Moon J S Son I CSong W K Moon T Hyeon J Am Chem Soc 2008 130 15573 [32] H W Gu R K Zheng X X Zhang B Xu J Am Chem Soc 2004 126 5664
66
P Sun H Zhang C Liu Langmuir 2010 26(2) 1278ndash1284 50
Immuno-labeling using Fe3O4CdTe nanocomposites which are formed by
linking multiple TGA-stabilized CdTe QDs with the thiol-functionalized silica
coated iron oxide nanoparticles
23-dimercaptosuccinic
acids (DMSA)Dopamine
Mercaptoundecanoic acid
(MUA)
HS COOH
4 Small multifunctional molecules
Glutaraldehyde 51
C J Xu K M Xu H W Gu R K Zheng H Liu X X Zhang Z H Guo B Xu J Am Chem Soc 2004 126 9938
Dopamine
Conditions (a) NaOH tert-butyl dicarbonate dioxane H2O 24 hrs (b) BnBr K2CO3 DMF
RT 24 h (c) 10 CF3COOH CH2Cl2 rt 5 h (d) succinic anhydride pyridine RTt 3 h (e)
NHS DCC DMAP CHCl3 RTt 3 h
NTA
52
3-mercapto trimethoxysilane
(MPS)
3-amino trimethoxysilane
(APS)
5 Silane multifunctional monomers
Glycidoxypropyltrimethoxy-silane
(GPS)
53
54
Multifunctional magnetic nanoparticles
application
Bioseparation
Biodetection(Real time monitoring)
Targeted drug delivery
Magnetic fluid Hyperthermia therapy
55
1 Specific Bio-Separation
bull Magnetic properties
bull Targeting agent
Cell organism molecule
56
His-tagged protein purification as
the first step towards recombinant
protein purification
58
Lysing Binding
Nickel
affinity
nanoparicle
His tag
fusion protein
Washing Elusion
Digestion
Binding
Nickel
affinity
nanoparticle
Washing Target
Recombinant
Protein
1- Preparation of Fe3O4SiO2Layered Double Hydroxide CoreminusShell
Microspheres for Magnetic Separation of Proteins J Am ChemSoc (2012)
18 134(2) 1071-7
Ni2+
2- Magnetic Nanocomposite Spheres Decorated with NiO Nanoparticles for a
Magnetically Recyclable Protein Separation System J Kim Y Piao N Lee Y
Park I-H Lee J-H Lee Adv Mater (2010) 22 57ndash60
His-tagged protein purification
Purification of His6-lipase using Fe3O4AundashLAndashIDAndashCu2+ NPs with various
amounts of NPs (lane 1 01 mg lane 2 02 mg lane 3
63
Synthetic procedure for the coreshell structure spheres
64
glutaraldehyde
(GLH)
Reference[1] H W Gu Z M Yang J H Gao C K Chang B Xu J Am Chem Soc 2005 127 34
[2] A Hutten D Sudfeld I Ennen G Reiss K Wojczykowski P Jutzi J Magn Magn Mater 2005
293 93
[3] G S Chaubey C Barcena N Poudyal C B Rong J M Gao S Sun J P Liu J Am Chem
Soc 2007 129 7214
[4] C W Kim Y H Kim H G Cha D K Lee Y S Kang J NanosciNanotechnol 2006 6 3417
[5] Y W Jun J S Choi J Cheon Angew Chem Int Ed 2006 45 3414
[6] X Michalet F F Pinaud L A Bentolila J M Tsay S Doose J J Li G Sundaresan A M Wu
S S Gambhir S Weiss Science 2005 307 538
[7] M Hu J Y Chen Z Y Li L Au G V Hartland X D Li M Marquez Y N Xia Chem Soc
Rev 2006 35 1084
[8] M Vallet-Regi F Balas D Arcos Angew Chem Int Ed 2007 46 7548
[9] S T Selvan P K Patra C Y Ang J Y Ying Angew Chem Int Ed 2007 46 2448
[10] I S Lee N Lee J Park B H Kim Y W Yi T Kim T K Kim I H Lee S R Paik T
Hyeon J Am Chem Soc 2006 128 10658
[11] Y S Lin S H Wu Y Hung Y H Chou C Chang M L Lin C P Tsai C Y Mou Chem
Mater 2006 18 5170
[12] B H Wu H Zhang C Chen S C Lin N F Zheng Nano Res 2009 2 975
[13] J Kim J E Lee J Lee J H Yu B C Kim K An Y Hwang C Shin J G Park J Kim T
Hyeon J Am Chem Soc 2006 128 688
[14] S Santra H S Yang P H Holloway J T Stanley R A Mericle J Am Chem Soc 2005 127
1656
[15] D K Yi S T Selvan S S Lee G C Papaefthymiou D Kundaliya J Y Ying J Am Chem
Soc 2005 127 4990 65
[16] J H Gao B Zhang Y Gao Y Pan X X Zhang B Xu J Am Chem Soc 2007 129 11928 [17] J H Gao W Zhang P B Huang B Zhang X X Zhang B Xu J Am Chem Soc 2008 130 3710 [18] J H Gao G L Liang B Zhang Y Kuang X X Zhang B Xu J Am Chem Soc 2007 129 1428 [19] J H Gao G L Liang J S Cheung Y Pan Y Kuang F Zhao B Zhang X X Zhang E X Wu B Xu J Am Chem Soc 2008 130 11828 [20] A R Kortan R Hull R L Opila M G Bawendi M L Steigerwald P J Carroll L E Brus JAm Chem Soc 1990 112 1327 [21] X G Peng M C Schlamp A V Kadavanich A P Alivisatos J Am Chem Soc 1997 119 7019 [22] S Kim B Fisher H J Eisler M Bawendi J Am Chem Soc 2003 125 11466[23] H Zeng J Li Z L Wang J P Liu S Sun Nano Lett 2004 4 187 [24] H Zeng S Sun J Li Z L Wang J P Liu Appl Phys Lett 2004 85 792 [25] Z Xu Y Hou S Sun J Am Chem Soc 2007 129 8698 [26] Y H Lin N Kim J Q Wang C J Zhong J Phys Chem B 2005 109 21593 [27] C Wang H Daimon S Sun Nano Lett 2009 9 1493 [28] K W Kwon M Shim J Am Chem Soc 2005 127 10269 [29] H Yu M Chen P M Rice S X Wang R L White S Sun Nano Lett 2005 5 379 [30] J S Choi Y W Jun S I Yeon H C Kim J S Shin J Cheon J Am Chem Soc 2006 128 15982 [31] S H Choi H B Na Y I Park K An S G Kwon Y Jang M Park J Moon J S Son I CSong W K Moon T Hyeon J Am Chem Soc 2008 130 15573 [32] H W Gu R K Zheng X X Zhang B Xu J Am Chem Soc 2004 126 5664
66
23-dimercaptosuccinic
acids (DMSA)Dopamine
Mercaptoundecanoic acid
(MUA)
HS COOH
4 Small multifunctional molecules
Glutaraldehyde 51
C J Xu K M Xu H W Gu R K Zheng H Liu X X Zhang Z H Guo B Xu J Am Chem Soc 2004 126 9938
Dopamine
Conditions (a) NaOH tert-butyl dicarbonate dioxane H2O 24 hrs (b) BnBr K2CO3 DMF
RT 24 h (c) 10 CF3COOH CH2Cl2 rt 5 h (d) succinic anhydride pyridine RTt 3 h (e)
NHS DCC DMAP CHCl3 RTt 3 h
NTA
52
3-mercapto trimethoxysilane
(MPS)
3-amino trimethoxysilane
(APS)
5 Silane multifunctional monomers
Glycidoxypropyltrimethoxy-silane
(GPS)
53
54
Multifunctional magnetic nanoparticles
application
Bioseparation
Biodetection(Real time monitoring)
Targeted drug delivery
Magnetic fluid Hyperthermia therapy
55
1 Specific Bio-Separation
bull Magnetic properties
bull Targeting agent
Cell organism molecule
56
His-tagged protein purification as
the first step towards recombinant
protein purification
58
Lysing Binding
Nickel
affinity
nanoparicle
His tag
fusion protein
Washing Elusion
Digestion
Binding
Nickel
affinity
nanoparticle
Washing Target
Recombinant
Protein
1- Preparation of Fe3O4SiO2Layered Double Hydroxide CoreminusShell
Microspheres for Magnetic Separation of Proteins J Am ChemSoc (2012)
18 134(2) 1071-7
Ni2+
2- Magnetic Nanocomposite Spheres Decorated with NiO Nanoparticles for a
Magnetically Recyclable Protein Separation System J Kim Y Piao N Lee Y
Park I-H Lee J-H Lee Adv Mater (2010) 22 57ndash60
His-tagged protein purification
Purification of His6-lipase using Fe3O4AundashLAndashIDAndashCu2+ NPs with various
amounts of NPs (lane 1 01 mg lane 2 02 mg lane 3
63
Synthetic procedure for the coreshell structure spheres
64
glutaraldehyde
(GLH)
Reference[1] H W Gu Z M Yang J H Gao C K Chang B Xu J Am Chem Soc 2005 127 34
[2] A Hutten D Sudfeld I Ennen G Reiss K Wojczykowski P Jutzi J Magn Magn Mater 2005
293 93
[3] G S Chaubey C Barcena N Poudyal C B Rong J M Gao S Sun J P Liu J Am Chem
Soc 2007 129 7214
[4] C W Kim Y H Kim H G Cha D K Lee Y S Kang J NanosciNanotechnol 2006 6 3417
[5] Y W Jun J S Choi J Cheon Angew Chem Int Ed 2006 45 3414
[6] X Michalet F F Pinaud L A Bentolila J M Tsay S Doose J J Li G Sundaresan A M Wu
S S Gambhir S Weiss Science 2005 307 538
[7] M Hu J Y Chen Z Y Li L Au G V Hartland X D Li M Marquez Y N Xia Chem Soc
Rev 2006 35 1084
[8] M Vallet-Regi F Balas D Arcos Angew Chem Int Ed 2007 46 7548
[9] S T Selvan P K Patra C Y Ang J Y Ying Angew Chem Int Ed 2007 46 2448
[10] I S Lee N Lee J Park B H Kim Y W Yi T Kim T K Kim I H Lee S R Paik T
Hyeon J Am Chem Soc 2006 128 10658
[11] Y S Lin S H Wu Y Hung Y H Chou C Chang M L Lin C P Tsai C Y Mou Chem
Mater 2006 18 5170
[12] B H Wu H Zhang C Chen S C Lin N F Zheng Nano Res 2009 2 975
[13] J Kim J E Lee J Lee J H Yu B C Kim K An Y Hwang C Shin J G Park J Kim T
Hyeon J Am Chem Soc 2006 128 688
[14] S Santra H S Yang P H Holloway J T Stanley R A Mericle J Am Chem Soc 2005 127
1656
[15] D K Yi S T Selvan S S Lee G C Papaefthymiou D Kundaliya J Y Ying J Am Chem
Soc 2005 127 4990 65
[16] J H Gao B Zhang Y Gao Y Pan X X Zhang B Xu J Am Chem Soc 2007 129 11928 [17] J H Gao W Zhang P B Huang B Zhang X X Zhang B Xu J Am Chem Soc 2008 130 3710 [18] J H Gao G L Liang B Zhang Y Kuang X X Zhang B Xu J Am Chem Soc 2007 129 1428 [19] J H Gao G L Liang J S Cheung Y Pan Y Kuang F Zhao B Zhang X X Zhang E X Wu B Xu J Am Chem Soc 2008 130 11828 [20] A R Kortan R Hull R L Opila M G Bawendi M L Steigerwald P J Carroll L E Brus JAm Chem Soc 1990 112 1327 [21] X G Peng M C Schlamp A V Kadavanich A P Alivisatos J Am Chem Soc 1997 119 7019 [22] S Kim B Fisher H J Eisler M Bawendi J Am Chem Soc 2003 125 11466[23] H Zeng J Li Z L Wang J P Liu S Sun Nano Lett 2004 4 187 [24] H Zeng S Sun J Li Z L Wang J P Liu Appl Phys Lett 2004 85 792 [25] Z Xu Y Hou S Sun J Am Chem Soc 2007 129 8698 [26] Y H Lin N Kim J Q Wang C J Zhong J Phys Chem B 2005 109 21593 [27] C Wang H Daimon S Sun Nano Lett 2009 9 1493 [28] K W Kwon M Shim J Am Chem Soc 2005 127 10269 [29] H Yu M Chen P M Rice S X Wang R L White S Sun Nano Lett 2005 5 379 [30] J S Choi Y W Jun S I Yeon H C Kim J S Shin J Cheon J Am Chem Soc 2006 128 15982 [31] S H Choi H B Na Y I Park K An S G Kwon Y Jang M Park J Moon J S Son I CSong W K Moon T Hyeon J Am Chem Soc 2008 130 15573 [32] H W Gu R K Zheng X X Zhang B Xu J Am Chem Soc 2004 126 5664
66
C J Xu K M Xu H W Gu R K Zheng H Liu X X Zhang Z H Guo B Xu J Am Chem Soc 2004 126 9938
Dopamine
Conditions (a) NaOH tert-butyl dicarbonate dioxane H2O 24 hrs (b) BnBr K2CO3 DMF
RT 24 h (c) 10 CF3COOH CH2Cl2 rt 5 h (d) succinic anhydride pyridine RTt 3 h (e)
NHS DCC DMAP CHCl3 RTt 3 h
NTA
52
3-mercapto trimethoxysilane
(MPS)
3-amino trimethoxysilane
(APS)
5 Silane multifunctional monomers
Glycidoxypropyltrimethoxy-silane
(GPS)
53
54
Multifunctional magnetic nanoparticles
application
Bioseparation
Biodetection(Real time monitoring)
Targeted drug delivery
Magnetic fluid Hyperthermia therapy
55
1 Specific Bio-Separation
bull Magnetic properties
bull Targeting agent
Cell organism molecule
56
His-tagged protein purification as
the first step towards recombinant
protein purification
58
Lysing Binding
Nickel
affinity
nanoparicle
His tag
fusion protein
Washing Elusion
Digestion
Binding
Nickel
affinity
nanoparticle
Washing Target
Recombinant
Protein
1- Preparation of Fe3O4SiO2Layered Double Hydroxide CoreminusShell
Microspheres for Magnetic Separation of Proteins J Am ChemSoc (2012)
18 134(2) 1071-7
Ni2+
2- Magnetic Nanocomposite Spheres Decorated with NiO Nanoparticles for a
Magnetically Recyclable Protein Separation System J Kim Y Piao N Lee Y
Park I-H Lee J-H Lee Adv Mater (2010) 22 57ndash60
His-tagged protein purification
Purification of His6-lipase using Fe3O4AundashLAndashIDAndashCu2+ NPs with various
amounts of NPs (lane 1 01 mg lane 2 02 mg lane 3
63
Synthetic procedure for the coreshell structure spheres
64
glutaraldehyde
(GLH)
Reference[1] H W Gu Z M Yang J H Gao C K Chang B Xu J Am Chem Soc 2005 127 34
[2] A Hutten D Sudfeld I Ennen G Reiss K Wojczykowski P Jutzi J Magn Magn Mater 2005
293 93
[3] G S Chaubey C Barcena N Poudyal C B Rong J M Gao S Sun J P Liu J Am Chem
Soc 2007 129 7214
[4] C W Kim Y H Kim H G Cha D K Lee Y S Kang J NanosciNanotechnol 2006 6 3417
[5] Y W Jun J S Choi J Cheon Angew Chem Int Ed 2006 45 3414
[6] X Michalet F F Pinaud L A Bentolila J M Tsay S Doose J J Li G Sundaresan A M Wu
S S Gambhir S Weiss Science 2005 307 538
[7] M Hu J Y Chen Z Y Li L Au G V Hartland X D Li M Marquez Y N Xia Chem Soc
Rev 2006 35 1084
[8] M Vallet-Regi F Balas D Arcos Angew Chem Int Ed 2007 46 7548
[9] S T Selvan P K Patra C Y Ang J Y Ying Angew Chem Int Ed 2007 46 2448
[10] I S Lee N Lee J Park B H Kim Y W Yi T Kim T K Kim I H Lee S R Paik T
Hyeon J Am Chem Soc 2006 128 10658
[11] Y S Lin S H Wu Y Hung Y H Chou C Chang M L Lin C P Tsai C Y Mou Chem
Mater 2006 18 5170
[12] B H Wu H Zhang C Chen S C Lin N F Zheng Nano Res 2009 2 975
[13] J Kim J E Lee J Lee J H Yu B C Kim K An Y Hwang C Shin J G Park J Kim T
Hyeon J Am Chem Soc 2006 128 688
[14] S Santra H S Yang P H Holloway J T Stanley R A Mericle J Am Chem Soc 2005 127
1656
[15] D K Yi S T Selvan S S Lee G C Papaefthymiou D Kundaliya J Y Ying J Am Chem
Soc 2005 127 4990 65
[16] J H Gao B Zhang Y Gao Y Pan X X Zhang B Xu J Am Chem Soc 2007 129 11928 [17] J H Gao W Zhang P B Huang B Zhang X X Zhang B Xu J Am Chem Soc 2008 130 3710 [18] J H Gao G L Liang B Zhang Y Kuang X X Zhang B Xu J Am Chem Soc 2007 129 1428 [19] J H Gao G L Liang J S Cheung Y Pan Y Kuang F Zhao B Zhang X X Zhang E X Wu B Xu J Am Chem Soc 2008 130 11828 [20] A R Kortan R Hull R L Opila M G Bawendi M L Steigerwald P J Carroll L E Brus JAm Chem Soc 1990 112 1327 [21] X G Peng M C Schlamp A V Kadavanich A P Alivisatos J Am Chem Soc 1997 119 7019 [22] S Kim B Fisher H J Eisler M Bawendi J Am Chem Soc 2003 125 11466[23] H Zeng J Li Z L Wang J P Liu S Sun Nano Lett 2004 4 187 [24] H Zeng S Sun J Li Z L Wang J P Liu Appl Phys Lett 2004 85 792 [25] Z Xu Y Hou S Sun J Am Chem Soc 2007 129 8698 [26] Y H Lin N Kim J Q Wang C J Zhong J Phys Chem B 2005 109 21593 [27] C Wang H Daimon S Sun Nano Lett 2009 9 1493 [28] K W Kwon M Shim J Am Chem Soc 2005 127 10269 [29] H Yu M Chen P M Rice S X Wang R L White S Sun Nano Lett 2005 5 379 [30] J S Choi Y W Jun S I Yeon H C Kim J S Shin J Cheon J Am Chem Soc 2006 128 15982 [31] S H Choi H B Na Y I Park K An S G Kwon Y Jang M Park J Moon J S Son I CSong W K Moon T Hyeon J Am Chem Soc 2008 130 15573 [32] H W Gu R K Zheng X X Zhang B Xu J Am Chem Soc 2004 126 5664
66
3-mercapto trimethoxysilane
(MPS)
3-amino trimethoxysilane
(APS)
5 Silane multifunctional monomers
Glycidoxypropyltrimethoxy-silane
(GPS)
53
54
Multifunctional magnetic nanoparticles
application
Bioseparation
Biodetection(Real time monitoring)
Targeted drug delivery
Magnetic fluid Hyperthermia therapy
55
1 Specific Bio-Separation
bull Magnetic properties
bull Targeting agent
Cell organism molecule
56
His-tagged protein purification as
the first step towards recombinant
protein purification
58
Lysing Binding
Nickel
affinity
nanoparicle
His tag
fusion protein
Washing Elusion
Digestion
Binding
Nickel
affinity
nanoparticle
Washing Target
Recombinant
Protein
1- Preparation of Fe3O4SiO2Layered Double Hydroxide CoreminusShell
Microspheres for Magnetic Separation of Proteins J Am ChemSoc (2012)
18 134(2) 1071-7
Ni2+
2- Magnetic Nanocomposite Spheres Decorated with NiO Nanoparticles for a
Magnetically Recyclable Protein Separation System J Kim Y Piao N Lee Y
Park I-H Lee J-H Lee Adv Mater (2010) 22 57ndash60
His-tagged protein purification
Purification of His6-lipase using Fe3O4AundashLAndashIDAndashCu2+ NPs with various
amounts of NPs (lane 1 01 mg lane 2 02 mg lane 3
63
Synthetic procedure for the coreshell structure spheres
64
glutaraldehyde
(GLH)
Reference[1] H W Gu Z M Yang J H Gao C K Chang B Xu J Am Chem Soc 2005 127 34
[2] A Hutten D Sudfeld I Ennen G Reiss K Wojczykowski P Jutzi J Magn Magn Mater 2005
293 93
[3] G S Chaubey C Barcena N Poudyal C B Rong J M Gao S Sun J P Liu J Am Chem
Soc 2007 129 7214
[4] C W Kim Y H Kim H G Cha D K Lee Y S Kang J NanosciNanotechnol 2006 6 3417
[5] Y W Jun J S Choi J Cheon Angew Chem Int Ed 2006 45 3414
[6] X Michalet F F Pinaud L A Bentolila J M Tsay S Doose J J Li G Sundaresan A M Wu
S S Gambhir S Weiss Science 2005 307 538
[7] M Hu J Y Chen Z Y Li L Au G V Hartland X D Li M Marquez Y N Xia Chem Soc
Rev 2006 35 1084
[8] M Vallet-Regi F Balas D Arcos Angew Chem Int Ed 2007 46 7548
[9] S T Selvan P K Patra C Y Ang J Y Ying Angew Chem Int Ed 2007 46 2448
[10] I S Lee N Lee J Park B H Kim Y W Yi T Kim T K Kim I H Lee S R Paik T
Hyeon J Am Chem Soc 2006 128 10658
[11] Y S Lin S H Wu Y Hung Y H Chou C Chang M L Lin C P Tsai C Y Mou Chem
Mater 2006 18 5170
[12] B H Wu H Zhang C Chen S C Lin N F Zheng Nano Res 2009 2 975
[13] J Kim J E Lee J Lee J H Yu B C Kim K An Y Hwang C Shin J G Park J Kim T
Hyeon J Am Chem Soc 2006 128 688
[14] S Santra H S Yang P H Holloway J T Stanley R A Mericle J Am Chem Soc 2005 127
1656
[15] D K Yi S T Selvan S S Lee G C Papaefthymiou D Kundaliya J Y Ying J Am Chem
Soc 2005 127 4990 65
[16] J H Gao B Zhang Y Gao Y Pan X X Zhang B Xu J Am Chem Soc 2007 129 11928 [17] J H Gao W Zhang P B Huang B Zhang X X Zhang B Xu J Am Chem Soc 2008 130 3710 [18] J H Gao G L Liang B Zhang Y Kuang X X Zhang B Xu J Am Chem Soc 2007 129 1428 [19] J H Gao G L Liang J S Cheung Y Pan Y Kuang F Zhao B Zhang X X Zhang E X Wu B Xu J Am Chem Soc 2008 130 11828 [20] A R Kortan R Hull R L Opila M G Bawendi M L Steigerwald P J Carroll L E Brus JAm Chem Soc 1990 112 1327 [21] X G Peng M C Schlamp A V Kadavanich A P Alivisatos J Am Chem Soc 1997 119 7019 [22] S Kim B Fisher H J Eisler M Bawendi J Am Chem Soc 2003 125 11466[23] H Zeng J Li Z L Wang J P Liu S Sun Nano Lett 2004 4 187 [24] H Zeng S Sun J Li Z L Wang J P Liu Appl Phys Lett 2004 85 792 [25] Z Xu Y Hou S Sun J Am Chem Soc 2007 129 8698 [26] Y H Lin N Kim J Q Wang C J Zhong J Phys Chem B 2005 109 21593 [27] C Wang H Daimon S Sun Nano Lett 2009 9 1493 [28] K W Kwon M Shim J Am Chem Soc 2005 127 10269 [29] H Yu M Chen P M Rice S X Wang R L White S Sun Nano Lett 2005 5 379 [30] J S Choi Y W Jun S I Yeon H C Kim J S Shin J Cheon J Am Chem Soc 2006 128 15982 [31] S H Choi H B Na Y I Park K An S G Kwon Y Jang M Park J Moon J S Son I CSong W K Moon T Hyeon J Am Chem Soc 2008 130 15573 [32] H W Gu R K Zheng X X Zhang B Xu J Am Chem Soc 2004 126 5664
66
54
Multifunctional magnetic nanoparticles
application
Bioseparation
Biodetection(Real time monitoring)
Targeted drug delivery
Magnetic fluid Hyperthermia therapy
55
1 Specific Bio-Separation
bull Magnetic properties
bull Targeting agent
Cell organism molecule
56
His-tagged protein purification as
the first step towards recombinant
protein purification
58
Lysing Binding
Nickel
affinity
nanoparicle
His tag
fusion protein
Washing Elusion
Digestion
Binding
Nickel
affinity
nanoparticle
Washing Target
Recombinant
Protein
1- Preparation of Fe3O4SiO2Layered Double Hydroxide CoreminusShell
Microspheres for Magnetic Separation of Proteins J Am ChemSoc (2012)
18 134(2) 1071-7
Ni2+
2- Magnetic Nanocomposite Spheres Decorated with NiO Nanoparticles for a
Magnetically Recyclable Protein Separation System J Kim Y Piao N Lee Y
Park I-H Lee J-H Lee Adv Mater (2010) 22 57ndash60
His-tagged protein purification
Purification of His6-lipase using Fe3O4AundashLAndashIDAndashCu2+ NPs with various
amounts of NPs (lane 1 01 mg lane 2 02 mg lane 3
63
Synthetic procedure for the coreshell structure spheres
64
glutaraldehyde
(GLH)
Reference[1] H W Gu Z M Yang J H Gao C K Chang B Xu J Am Chem Soc 2005 127 34
[2] A Hutten D Sudfeld I Ennen G Reiss K Wojczykowski P Jutzi J Magn Magn Mater 2005
293 93
[3] G S Chaubey C Barcena N Poudyal C B Rong J M Gao S Sun J P Liu J Am Chem
Soc 2007 129 7214
[4] C W Kim Y H Kim H G Cha D K Lee Y S Kang J NanosciNanotechnol 2006 6 3417
[5] Y W Jun J S Choi J Cheon Angew Chem Int Ed 2006 45 3414
[6] X Michalet F F Pinaud L A Bentolila J M Tsay S Doose J J Li G Sundaresan A M Wu
S S Gambhir S Weiss Science 2005 307 538
[7] M Hu J Y Chen Z Y Li L Au G V Hartland X D Li M Marquez Y N Xia Chem Soc
Rev 2006 35 1084
[8] M Vallet-Regi F Balas D Arcos Angew Chem Int Ed 2007 46 7548
[9] S T Selvan P K Patra C Y Ang J Y Ying Angew Chem Int Ed 2007 46 2448
[10] I S Lee N Lee J Park B H Kim Y W Yi T Kim T K Kim I H Lee S R Paik T
Hyeon J Am Chem Soc 2006 128 10658
[11] Y S Lin S H Wu Y Hung Y H Chou C Chang M L Lin C P Tsai C Y Mou Chem
Mater 2006 18 5170
[12] B H Wu H Zhang C Chen S C Lin N F Zheng Nano Res 2009 2 975
[13] J Kim J E Lee J Lee J H Yu B C Kim K An Y Hwang C Shin J G Park J Kim T
Hyeon J Am Chem Soc 2006 128 688
[14] S Santra H S Yang P H Holloway J T Stanley R A Mericle J Am Chem Soc 2005 127
1656
[15] D K Yi S T Selvan S S Lee G C Papaefthymiou D Kundaliya J Y Ying J Am Chem
Soc 2005 127 4990 65
[16] J H Gao B Zhang Y Gao Y Pan X X Zhang B Xu J Am Chem Soc 2007 129 11928 [17] J H Gao W Zhang P B Huang B Zhang X X Zhang B Xu J Am Chem Soc 2008 130 3710 [18] J H Gao G L Liang B Zhang Y Kuang X X Zhang B Xu J Am Chem Soc 2007 129 1428 [19] J H Gao G L Liang J S Cheung Y Pan Y Kuang F Zhao B Zhang X X Zhang E X Wu B Xu J Am Chem Soc 2008 130 11828 [20] A R Kortan R Hull R L Opila M G Bawendi M L Steigerwald P J Carroll L E Brus JAm Chem Soc 1990 112 1327 [21] X G Peng M C Schlamp A V Kadavanich A P Alivisatos J Am Chem Soc 1997 119 7019 [22] S Kim B Fisher H J Eisler M Bawendi J Am Chem Soc 2003 125 11466[23] H Zeng J Li Z L Wang J P Liu S Sun Nano Lett 2004 4 187 [24] H Zeng S Sun J Li Z L Wang J P Liu Appl Phys Lett 2004 85 792 [25] Z Xu Y Hou S Sun J Am Chem Soc 2007 129 8698 [26] Y H Lin N Kim J Q Wang C J Zhong J Phys Chem B 2005 109 21593 [27] C Wang H Daimon S Sun Nano Lett 2009 9 1493 [28] K W Kwon M Shim J Am Chem Soc 2005 127 10269 [29] H Yu M Chen P M Rice S X Wang R L White S Sun Nano Lett 2005 5 379 [30] J S Choi Y W Jun S I Yeon H C Kim J S Shin J Cheon J Am Chem Soc 2006 128 15982 [31] S H Choi H B Na Y I Park K An S G Kwon Y Jang M Park J Moon J S Son I CSong W K Moon T Hyeon J Am Chem Soc 2008 130 15573 [32] H W Gu R K Zheng X X Zhang B Xu J Am Chem Soc 2004 126 5664
66
Multifunctional magnetic nanoparticles
application
Bioseparation
Biodetection(Real time monitoring)
Targeted drug delivery
Magnetic fluid Hyperthermia therapy
55
1 Specific Bio-Separation
bull Magnetic properties
bull Targeting agent
Cell organism molecule
56
His-tagged protein purification as
the first step towards recombinant
protein purification
58
Lysing Binding
Nickel
affinity
nanoparicle
His tag
fusion protein
Washing Elusion
Digestion
Binding
Nickel
affinity
nanoparticle
Washing Target
Recombinant
Protein
1- Preparation of Fe3O4SiO2Layered Double Hydroxide CoreminusShell
Microspheres for Magnetic Separation of Proteins J Am ChemSoc (2012)
18 134(2) 1071-7
Ni2+
2- Magnetic Nanocomposite Spheres Decorated with NiO Nanoparticles for a
Magnetically Recyclable Protein Separation System J Kim Y Piao N Lee Y
Park I-H Lee J-H Lee Adv Mater (2010) 22 57ndash60
His-tagged protein purification
Purification of His6-lipase using Fe3O4AundashLAndashIDAndashCu2+ NPs with various
amounts of NPs (lane 1 01 mg lane 2 02 mg lane 3
63
Synthetic procedure for the coreshell structure spheres
64
glutaraldehyde
(GLH)
Reference[1] H W Gu Z M Yang J H Gao C K Chang B Xu J Am Chem Soc 2005 127 34
[2] A Hutten D Sudfeld I Ennen G Reiss K Wojczykowski P Jutzi J Magn Magn Mater 2005
293 93
[3] G S Chaubey C Barcena N Poudyal C B Rong J M Gao S Sun J P Liu J Am Chem
Soc 2007 129 7214
[4] C W Kim Y H Kim H G Cha D K Lee Y S Kang J NanosciNanotechnol 2006 6 3417
[5] Y W Jun J S Choi J Cheon Angew Chem Int Ed 2006 45 3414
[6] X Michalet F F Pinaud L A Bentolila J M Tsay S Doose J J Li G Sundaresan A M Wu
S S Gambhir S Weiss Science 2005 307 538
[7] M Hu J Y Chen Z Y Li L Au G V Hartland X D Li M Marquez Y N Xia Chem Soc
Rev 2006 35 1084
[8] M Vallet-Regi F Balas D Arcos Angew Chem Int Ed 2007 46 7548
[9] S T Selvan P K Patra C Y Ang J Y Ying Angew Chem Int Ed 2007 46 2448
[10] I S Lee N Lee J Park B H Kim Y W Yi T Kim T K Kim I H Lee S R Paik T
Hyeon J Am Chem Soc 2006 128 10658
[11] Y S Lin S H Wu Y Hung Y H Chou C Chang M L Lin C P Tsai C Y Mou Chem
Mater 2006 18 5170
[12] B H Wu H Zhang C Chen S C Lin N F Zheng Nano Res 2009 2 975
[13] J Kim J E Lee J Lee J H Yu B C Kim K An Y Hwang C Shin J G Park J Kim T
Hyeon J Am Chem Soc 2006 128 688
[14] S Santra H S Yang P H Holloway J T Stanley R A Mericle J Am Chem Soc 2005 127
1656
[15] D K Yi S T Selvan S S Lee G C Papaefthymiou D Kundaliya J Y Ying J Am Chem
Soc 2005 127 4990 65
[16] J H Gao B Zhang Y Gao Y Pan X X Zhang B Xu J Am Chem Soc 2007 129 11928 [17] J H Gao W Zhang P B Huang B Zhang X X Zhang B Xu J Am Chem Soc 2008 130 3710 [18] J H Gao G L Liang B Zhang Y Kuang X X Zhang B Xu J Am Chem Soc 2007 129 1428 [19] J H Gao G L Liang J S Cheung Y Pan Y Kuang F Zhao B Zhang X X Zhang E X Wu B Xu J Am Chem Soc 2008 130 11828 [20] A R Kortan R Hull R L Opila M G Bawendi M L Steigerwald P J Carroll L E Brus JAm Chem Soc 1990 112 1327 [21] X G Peng M C Schlamp A V Kadavanich A P Alivisatos J Am Chem Soc 1997 119 7019 [22] S Kim B Fisher H J Eisler M Bawendi J Am Chem Soc 2003 125 11466[23] H Zeng J Li Z L Wang J P Liu S Sun Nano Lett 2004 4 187 [24] H Zeng S Sun J Li Z L Wang J P Liu Appl Phys Lett 2004 85 792 [25] Z Xu Y Hou S Sun J Am Chem Soc 2007 129 8698 [26] Y H Lin N Kim J Q Wang C J Zhong J Phys Chem B 2005 109 21593 [27] C Wang H Daimon S Sun Nano Lett 2009 9 1493 [28] K W Kwon M Shim J Am Chem Soc 2005 127 10269 [29] H Yu M Chen P M Rice S X Wang R L White S Sun Nano Lett 2005 5 379 [30] J S Choi Y W Jun S I Yeon H C Kim J S Shin J Cheon J Am Chem Soc 2006 128 15982 [31] S H Choi H B Na Y I Park K An S G Kwon Y Jang M Park J Moon J S Son I CSong W K Moon T Hyeon J Am Chem Soc 2008 130 15573 [32] H W Gu R K Zheng X X Zhang B Xu J Am Chem Soc 2004 126 5664
66
1 Specific Bio-Separation
bull Magnetic properties
bull Targeting agent
Cell organism molecule
56
His-tagged protein purification as
the first step towards recombinant
protein purification
58
Lysing Binding
Nickel
affinity
nanoparicle
His tag
fusion protein
Washing Elusion
Digestion
Binding
Nickel
affinity
nanoparticle
Washing Target
Recombinant
Protein
1- Preparation of Fe3O4SiO2Layered Double Hydroxide CoreminusShell
Microspheres for Magnetic Separation of Proteins J Am ChemSoc (2012)
18 134(2) 1071-7
Ni2+
2- Magnetic Nanocomposite Spheres Decorated with NiO Nanoparticles for a
Magnetically Recyclable Protein Separation System J Kim Y Piao N Lee Y
Park I-H Lee J-H Lee Adv Mater (2010) 22 57ndash60
His-tagged protein purification
Purification of His6-lipase using Fe3O4AundashLAndashIDAndashCu2+ NPs with various
amounts of NPs (lane 1 01 mg lane 2 02 mg lane 3
63
Synthetic procedure for the coreshell structure spheres
64
glutaraldehyde
(GLH)
Reference[1] H W Gu Z M Yang J H Gao C K Chang B Xu J Am Chem Soc 2005 127 34
[2] A Hutten D Sudfeld I Ennen G Reiss K Wojczykowski P Jutzi J Magn Magn Mater 2005
293 93
[3] G S Chaubey C Barcena N Poudyal C B Rong J M Gao S Sun J P Liu J Am Chem
Soc 2007 129 7214
[4] C W Kim Y H Kim H G Cha D K Lee Y S Kang J NanosciNanotechnol 2006 6 3417
[5] Y W Jun J S Choi J Cheon Angew Chem Int Ed 2006 45 3414
[6] X Michalet F F Pinaud L A Bentolila J M Tsay S Doose J J Li G Sundaresan A M Wu
S S Gambhir S Weiss Science 2005 307 538
[7] M Hu J Y Chen Z Y Li L Au G V Hartland X D Li M Marquez Y N Xia Chem Soc
Rev 2006 35 1084
[8] M Vallet-Regi F Balas D Arcos Angew Chem Int Ed 2007 46 7548
[9] S T Selvan P K Patra C Y Ang J Y Ying Angew Chem Int Ed 2007 46 2448
[10] I S Lee N Lee J Park B H Kim Y W Yi T Kim T K Kim I H Lee S R Paik T
Hyeon J Am Chem Soc 2006 128 10658
[11] Y S Lin S H Wu Y Hung Y H Chou C Chang M L Lin C P Tsai C Y Mou Chem
Mater 2006 18 5170
[12] B H Wu H Zhang C Chen S C Lin N F Zheng Nano Res 2009 2 975
[13] J Kim J E Lee J Lee J H Yu B C Kim K An Y Hwang C Shin J G Park J Kim T
Hyeon J Am Chem Soc 2006 128 688
[14] S Santra H S Yang P H Holloway J T Stanley R A Mericle J Am Chem Soc 2005 127
1656
[15] D K Yi S T Selvan S S Lee G C Papaefthymiou D Kundaliya J Y Ying J Am Chem
Soc 2005 127 4990 65
[16] J H Gao B Zhang Y Gao Y Pan X X Zhang B Xu J Am Chem Soc 2007 129 11928 [17] J H Gao W Zhang P B Huang B Zhang X X Zhang B Xu J Am Chem Soc 2008 130 3710 [18] J H Gao G L Liang B Zhang Y Kuang X X Zhang B Xu J Am Chem Soc 2007 129 1428 [19] J H Gao G L Liang J S Cheung Y Pan Y Kuang F Zhao B Zhang X X Zhang E X Wu B Xu J Am Chem Soc 2008 130 11828 [20] A R Kortan R Hull R L Opila M G Bawendi M L Steigerwald P J Carroll L E Brus JAm Chem Soc 1990 112 1327 [21] X G Peng M C Schlamp A V Kadavanich A P Alivisatos J Am Chem Soc 1997 119 7019 [22] S Kim B Fisher H J Eisler M Bawendi J Am Chem Soc 2003 125 11466[23] H Zeng J Li Z L Wang J P Liu S Sun Nano Lett 2004 4 187 [24] H Zeng S Sun J Li Z L Wang J P Liu Appl Phys Lett 2004 85 792 [25] Z Xu Y Hou S Sun J Am Chem Soc 2007 129 8698 [26] Y H Lin N Kim J Q Wang C J Zhong J Phys Chem B 2005 109 21593 [27] C Wang H Daimon S Sun Nano Lett 2009 9 1493 [28] K W Kwon M Shim J Am Chem Soc 2005 127 10269 [29] H Yu M Chen P M Rice S X Wang R L White S Sun Nano Lett 2005 5 379 [30] J S Choi Y W Jun S I Yeon H C Kim J S Shin J Cheon J Am Chem Soc 2006 128 15982 [31] S H Choi H B Na Y I Park K An S G Kwon Y Jang M Park J Moon J S Son I CSong W K Moon T Hyeon J Am Chem Soc 2008 130 15573 [32] H W Gu R K Zheng X X Zhang B Xu J Am Chem Soc 2004 126 5664
66
His-tagged protein purification as
the first step towards recombinant
protein purification
58
Lysing Binding
Nickel
affinity
nanoparicle
His tag
fusion protein
Washing Elusion
Digestion
Binding
Nickel
affinity
nanoparticle
Washing Target
Recombinant
Protein
1- Preparation of Fe3O4SiO2Layered Double Hydroxide CoreminusShell
Microspheres for Magnetic Separation of Proteins J Am ChemSoc (2012)
18 134(2) 1071-7
Ni2+
2- Magnetic Nanocomposite Spheres Decorated with NiO Nanoparticles for a
Magnetically Recyclable Protein Separation System J Kim Y Piao N Lee Y
Park I-H Lee J-H Lee Adv Mater (2010) 22 57ndash60
His-tagged protein purification
Purification of His6-lipase using Fe3O4AundashLAndashIDAndashCu2+ NPs with various
amounts of NPs (lane 1 01 mg lane 2 02 mg lane 3
63
Synthetic procedure for the coreshell structure spheres
64
glutaraldehyde
(GLH)
Reference[1] H W Gu Z M Yang J H Gao C K Chang B Xu J Am Chem Soc 2005 127 34
[2] A Hutten D Sudfeld I Ennen G Reiss K Wojczykowski P Jutzi J Magn Magn Mater 2005
293 93
[3] G S Chaubey C Barcena N Poudyal C B Rong J M Gao S Sun J P Liu J Am Chem
Soc 2007 129 7214
[4] C W Kim Y H Kim H G Cha D K Lee Y S Kang J NanosciNanotechnol 2006 6 3417
[5] Y W Jun J S Choi J Cheon Angew Chem Int Ed 2006 45 3414
[6] X Michalet F F Pinaud L A Bentolila J M Tsay S Doose J J Li G Sundaresan A M Wu
S S Gambhir S Weiss Science 2005 307 538
[7] M Hu J Y Chen Z Y Li L Au G V Hartland X D Li M Marquez Y N Xia Chem Soc
Rev 2006 35 1084
[8] M Vallet-Regi F Balas D Arcos Angew Chem Int Ed 2007 46 7548
[9] S T Selvan P K Patra C Y Ang J Y Ying Angew Chem Int Ed 2007 46 2448
[10] I S Lee N Lee J Park B H Kim Y W Yi T Kim T K Kim I H Lee S R Paik T
Hyeon J Am Chem Soc 2006 128 10658
[11] Y S Lin S H Wu Y Hung Y H Chou C Chang M L Lin C P Tsai C Y Mou Chem
Mater 2006 18 5170
[12] B H Wu H Zhang C Chen S C Lin N F Zheng Nano Res 2009 2 975
[13] J Kim J E Lee J Lee J H Yu B C Kim K An Y Hwang C Shin J G Park J Kim T
Hyeon J Am Chem Soc 2006 128 688
[14] S Santra H S Yang P H Holloway J T Stanley R A Mericle J Am Chem Soc 2005 127
1656
[15] D K Yi S T Selvan S S Lee G C Papaefthymiou D Kundaliya J Y Ying J Am Chem
Soc 2005 127 4990 65
[16] J H Gao B Zhang Y Gao Y Pan X X Zhang B Xu J Am Chem Soc 2007 129 11928 [17] J H Gao W Zhang P B Huang B Zhang X X Zhang B Xu J Am Chem Soc 2008 130 3710 [18] J H Gao G L Liang B Zhang Y Kuang X X Zhang B Xu J Am Chem Soc 2007 129 1428 [19] J H Gao G L Liang J S Cheung Y Pan Y Kuang F Zhao B Zhang X X Zhang E X Wu B Xu J Am Chem Soc 2008 130 11828 [20] A R Kortan R Hull R L Opila M G Bawendi M L Steigerwald P J Carroll L E Brus JAm Chem Soc 1990 112 1327 [21] X G Peng M C Schlamp A V Kadavanich A P Alivisatos J Am Chem Soc 1997 119 7019 [22] S Kim B Fisher H J Eisler M Bawendi J Am Chem Soc 2003 125 11466[23] H Zeng J Li Z L Wang J P Liu S Sun Nano Lett 2004 4 187 [24] H Zeng S Sun J Li Z L Wang J P Liu Appl Phys Lett 2004 85 792 [25] Z Xu Y Hou S Sun J Am Chem Soc 2007 129 8698 [26] Y H Lin N Kim J Q Wang C J Zhong J Phys Chem B 2005 109 21593 [27] C Wang H Daimon S Sun Nano Lett 2009 9 1493 [28] K W Kwon M Shim J Am Chem Soc 2005 127 10269 [29] H Yu M Chen P M Rice S X Wang R L White S Sun Nano Lett 2005 5 379 [30] J S Choi Y W Jun S I Yeon H C Kim J S Shin J Cheon J Am Chem Soc 2006 128 15982 [31] S H Choi H B Na Y I Park K An S G Kwon Y Jang M Park J Moon J S Son I CSong W K Moon T Hyeon J Am Chem Soc 2008 130 15573 [32] H W Gu R K Zheng X X Zhang B Xu J Am Chem Soc 2004 126 5664
66
58
Lysing Binding
Nickel
affinity
nanoparicle
His tag
fusion protein
Washing Elusion
Digestion
Binding
Nickel
affinity
nanoparticle
Washing Target
Recombinant
Protein
1- Preparation of Fe3O4SiO2Layered Double Hydroxide CoreminusShell
Microspheres for Magnetic Separation of Proteins J Am ChemSoc (2012)
18 134(2) 1071-7
Ni2+
2- Magnetic Nanocomposite Spheres Decorated with NiO Nanoparticles for a
Magnetically Recyclable Protein Separation System J Kim Y Piao N Lee Y
Park I-H Lee J-H Lee Adv Mater (2010) 22 57ndash60
His-tagged protein purification
Purification of His6-lipase using Fe3O4AundashLAndashIDAndashCu2+ NPs with various
amounts of NPs (lane 1 01 mg lane 2 02 mg lane 3
63
Synthetic procedure for the coreshell structure spheres
64
glutaraldehyde
(GLH)
Reference[1] H W Gu Z M Yang J H Gao C K Chang B Xu J Am Chem Soc 2005 127 34
[2] A Hutten D Sudfeld I Ennen G Reiss K Wojczykowski P Jutzi J Magn Magn Mater 2005
293 93
[3] G S Chaubey C Barcena N Poudyal C B Rong J M Gao S Sun J P Liu J Am Chem
Soc 2007 129 7214
[4] C W Kim Y H Kim H G Cha D K Lee Y S Kang J NanosciNanotechnol 2006 6 3417
[5] Y W Jun J S Choi J Cheon Angew Chem Int Ed 2006 45 3414
[6] X Michalet F F Pinaud L A Bentolila J M Tsay S Doose J J Li G Sundaresan A M Wu
S S Gambhir S Weiss Science 2005 307 538
[7] M Hu J Y Chen Z Y Li L Au G V Hartland X D Li M Marquez Y N Xia Chem Soc
Rev 2006 35 1084
[8] M Vallet-Regi F Balas D Arcos Angew Chem Int Ed 2007 46 7548
[9] S T Selvan P K Patra C Y Ang J Y Ying Angew Chem Int Ed 2007 46 2448
[10] I S Lee N Lee J Park B H Kim Y W Yi T Kim T K Kim I H Lee S R Paik T
Hyeon J Am Chem Soc 2006 128 10658
[11] Y S Lin S H Wu Y Hung Y H Chou C Chang M L Lin C P Tsai C Y Mou Chem
Mater 2006 18 5170
[12] B H Wu H Zhang C Chen S C Lin N F Zheng Nano Res 2009 2 975
[13] J Kim J E Lee J Lee J H Yu B C Kim K An Y Hwang C Shin J G Park J Kim T
Hyeon J Am Chem Soc 2006 128 688
[14] S Santra H S Yang P H Holloway J T Stanley R A Mericle J Am Chem Soc 2005 127
1656
[15] D K Yi S T Selvan S S Lee G C Papaefthymiou D Kundaliya J Y Ying J Am Chem
Soc 2005 127 4990 65
[16] J H Gao B Zhang Y Gao Y Pan X X Zhang B Xu J Am Chem Soc 2007 129 11928 [17] J H Gao W Zhang P B Huang B Zhang X X Zhang B Xu J Am Chem Soc 2008 130 3710 [18] J H Gao G L Liang B Zhang Y Kuang X X Zhang B Xu J Am Chem Soc 2007 129 1428 [19] J H Gao G L Liang J S Cheung Y Pan Y Kuang F Zhao B Zhang X X Zhang E X Wu B Xu J Am Chem Soc 2008 130 11828 [20] A R Kortan R Hull R L Opila M G Bawendi M L Steigerwald P J Carroll L E Brus JAm Chem Soc 1990 112 1327 [21] X G Peng M C Schlamp A V Kadavanich A P Alivisatos J Am Chem Soc 1997 119 7019 [22] S Kim B Fisher H J Eisler M Bawendi J Am Chem Soc 2003 125 11466[23] H Zeng J Li Z L Wang J P Liu S Sun Nano Lett 2004 4 187 [24] H Zeng S Sun J Li Z L Wang J P Liu Appl Phys Lett 2004 85 792 [25] Z Xu Y Hou S Sun J Am Chem Soc 2007 129 8698 [26] Y H Lin N Kim J Q Wang C J Zhong J Phys Chem B 2005 109 21593 [27] C Wang H Daimon S Sun Nano Lett 2009 9 1493 [28] K W Kwon M Shim J Am Chem Soc 2005 127 10269 [29] H Yu M Chen P M Rice S X Wang R L White S Sun Nano Lett 2005 5 379 [30] J S Choi Y W Jun S I Yeon H C Kim J S Shin J Cheon J Am Chem Soc 2006 128 15982 [31] S H Choi H B Na Y I Park K An S G Kwon Y Jang M Park J Moon J S Son I CSong W K Moon T Hyeon J Am Chem Soc 2008 130 15573 [32] H W Gu R K Zheng X X Zhang B Xu J Am Chem Soc 2004 126 5664
66
Lysing Binding
Nickel
affinity
nanoparicle
His tag
fusion protein
Washing Elusion
Digestion
Binding
Nickel
affinity
nanoparticle
Washing Target
Recombinant
Protein
1- Preparation of Fe3O4SiO2Layered Double Hydroxide CoreminusShell
Microspheres for Magnetic Separation of Proteins J Am ChemSoc (2012)
18 134(2) 1071-7
Ni2+
2- Magnetic Nanocomposite Spheres Decorated with NiO Nanoparticles for a
Magnetically Recyclable Protein Separation System J Kim Y Piao N Lee Y
Park I-H Lee J-H Lee Adv Mater (2010) 22 57ndash60
His-tagged protein purification
Purification of His6-lipase using Fe3O4AundashLAndashIDAndashCu2+ NPs with various
amounts of NPs (lane 1 01 mg lane 2 02 mg lane 3
63
Synthetic procedure for the coreshell structure spheres
64
glutaraldehyde
(GLH)
Reference[1] H W Gu Z M Yang J H Gao C K Chang B Xu J Am Chem Soc 2005 127 34
[2] A Hutten D Sudfeld I Ennen G Reiss K Wojczykowski P Jutzi J Magn Magn Mater 2005
293 93
[3] G S Chaubey C Barcena N Poudyal C B Rong J M Gao S Sun J P Liu J Am Chem
Soc 2007 129 7214
[4] C W Kim Y H Kim H G Cha D K Lee Y S Kang J NanosciNanotechnol 2006 6 3417
[5] Y W Jun J S Choi J Cheon Angew Chem Int Ed 2006 45 3414
[6] X Michalet F F Pinaud L A Bentolila J M Tsay S Doose J J Li G Sundaresan A M Wu
S S Gambhir S Weiss Science 2005 307 538
[7] M Hu J Y Chen Z Y Li L Au G V Hartland X D Li M Marquez Y N Xia Chem Soc
Rev 2006 35 1084
[8] M Vallet-Regi F Balas D Arcos Angew Chem Int Ed 2007 46 7548
[9] S T Selvan P K Patra C Y Ang J Y Ying Angew Chem Int Ed 2007 46 2448
[10] I S Lee N Lee J Park B H Kim Y W Yi T Kim T K Kim I H Lee S R Paik T
Hyeon J Am Chem Soc 2006 128 10658
[11] Y S Lin S H Wu Y Hung Y H Chou C Chang M L Lin C P Tsai C Y Mou Chem
Mater 2006 18 5170
[12] B H Wu H Zhang C Chen S C Lin N F Zheng Nano Res 2009 2 975
[13] J Kim J E Lee J Lee J H Yu B C Kim K An Y Hwang C Shin J G Park J Kim T
Hyeon J Am Chem Soc 2006 128 688
[14] S Santra H S Yang P H Holloway J T Stanley R A Mericle J Am Chem Soc 2005 127
1656
[15] D K Yi S T Selvan S S Lee G C Papaefthymiou D Kundaliya J Y Ying J Am Chem
Soc 2005 127 4990 65
[16] J H Gao B Zhang Y Gao Y Pan X X Zhang B Xu J Am Chem Soc 2007 129 11928 [17] J H Gao W Zhang P B Huang B Zhang X X Zhang B Xu J Am Chem Soc 2008 130 3710 [18] J H Gao G L Liang B Zhang Y Kuang X X Zhang B Xu J Am Chem Soc 2007 129 1428 [19] J H Gao G L Liang J S Cheung Y Pan Y Kuang F Zhao B Zhang X X Zhang E X Wu B Xu J Am Chem Soc 2008 130 11828 [20] A R Kortan R Hull R L Opila M G Bawendi M L Steigerwald P J Carroll L E Brus JAm Chem Soc 1990 112 1327 [21] X G Peng M C Schlamp A V Kadavanich A P Alivisatos J Am Chem Soc 1997 119 7019 [22] S Kim B Fisher H J Eisler M Bawendi J Am Chem Soc 2003 125 11466[23] H Zeng J Li Z L Wang J P Liu S Sun Nano Lett 2004 4 187 [24] H Zeng S Sun J Li Z L Wang J P Liu Appl Phys Lett 2004 85 792 [25] Z Xu Y Hou S Sun J Am Chem Soc 2007 129 8698 [26] Y H Lin N Kim J Q Wang C J Zhong J Phys Chem B 2005 109 21593 [27] C Wang H Daimon S Sun Nano Lett 2009 9 1493 [28] K W Kwon M Shim J Am Chem Soc 2005 127 10269 [29] H Yu M Chen P M Rice S X Wang R L White S Sun Nano Lett 2005 5 379 [30] J S Choi Y W Jun S I Yeon H C Kim J S Shin J Cheon J Am Chem Soc 2006 128 15982 [31] S H Choi H B Na Y I Park K An S G Kwon Y Jang M Park J Moon J S Son I CSong W K Moon T Hyeon J Am Chem Soc 2008 130 15573 [32] H W Gu R K Zheng X X Zhang B Xu J Am Chem Soc 2004 126 5664
66
Digestion
Binding
Nickel
affinity
nanoparticle
Washing Target
Recombinant
Protein
1- Preparation of Fe3O4SiO2Layered Double Hydroxide CoreminusShell
Microspheres for Magnetic Separation of Proteins J Am ChemSoc (2012)
18 134(2) 1071-7
Ni2+
2- Magnetic Nanocomposite Spheres Decorated with NiO Nanoparticles for a
Magnetically Recyclable Protein Separation System J Kim Y Piao N Lee Y
Park I-H Lee J-H Lee Adv Mater (2010) 22 57ndash60
His-tagged protein purification
Purification of His6-lipase using Fe3O4AundashLAndashIDAndashCu2+ NPs with various
amounts of NPs (lane 1 01 mg lane 2 02 mg lane 3
63
Synthetic procedure for the coreshell structure spheres
64
glutaraldehyde
(GLH)
Reference[1] H W Gu Z M Yang J H Gao C K Chang B Xu J Am Chem Soc 2005 127 34
[2] A Hutten D Sudfeld I Ennen G Reiss K Wojczykowski P Jutzi J Magn Magn Mater 2005
293 93
[3] G S Chaubey C Barcena N Poudyal C B Rong J M Gao S Sun J P Liu J Am Chem
Soc 2007 129 7214
[4] C W Kim Y H Kim H G Cha D K Lee Y S Kang J NanosciNanotechnol 2006 6 3417
[5] Y W Jun J S Choi J Cheon Angew Chem Int Ed 2006 45 3414
[6] X Michalet F F Pinaud L A Bentolila J M Tsay S Doose J J Li G Sundaresan A M Wu
S S Gambhir S Weiss Science 2005 307 538
[7] M Hu J Y Chen Z Y Li L Au G V Hartland X D Li M Marquez Y N Xia Chem Soc
Rev 2006 35 1084
[8] M Vallet-Regi F Balas D Arcos Angew Chem Int Ed 2007 46 7548
[9] S T Selvan P K Patra C Y Ang J Y Ying Angew Chem Int Ed 2007 46 2448
[10] I S Lee N Lee J Park B H Kim Y W Yi T Kim T K Kim I H Lee S R Paik T
Hyeon J Am Chem Soc 2006 128 10658
[11] Y S Lin S H Wu Y Hung Y H Chou C Chang M L Lin C P Tsai C Y Mou Chem
Mater 2006 18 5170
[12] B H Wu H Zhang C Chen S C Lin N F Zheng Nano Res 2009 2 975
[13] J Kim J E Lee J Lee J H Yu B C Kim K An Y Hwang C Shin J G Park J Kim T
Hyeon J Am Chem Soc 2006 128 688
[14] S Santra H S Yang P H Holloway J T Stanley R A Mericle J Am Chem Soc 2005 127
1656
[15] D K Yi S T Selvan S S Lee G C Papaefthymiou D Kundaliya J Y Ying J Am Chem
Soc 2005 127 4990 65
[16] J H Gao B Zhang Y Gao Y Pan X X Zhang B Xu J Am Chem Soc 2007 129 11928 [17] J H Gao W Zhang P B Huang B Zhang X X Zhang B Xu J Am Chem Soc 2008 130 3710 [18] J H Gao G L Liang B Zhang Y Kuang X X Zhang B Xu J Am Chem Soc 2007 129 1428 [19] J H Gao G L Liang J S Cheung Y Pan Y Kuang F Zhao B Zhang X X Zhang E X Wu B Xu J Am Chem Soc 2008 130 11828 [20] A R Kortan R Hull R L Opila M G Bawendi M L Steigerwald P J Carroll L E Brus JAm Chem Soc 1990 112 1327 [21] X G Peng M C Schlamp A V Kadavanich A P Alivisatos J Am Chem Soc 1997 119 7019 [22] S Kim B Fisher H J Eisler M Bawendi J Am Chem Soc 2003 125 11466[23] H Zeng J Li Z L Wang J P Liu S Sun Nano Lett 2004 4 187 [24] H Zeng S Sun J Li Z L Wang J P Liu Appl Phys Lett 2004 85 792 [25] Z Xu Y Hou S Sun J Am Chem Soc 2007 129 8698 [26] Y H Lin N Kim J Q Wang C J Zhong J Phys Chem B 2005 109 21593 [27] C Wang H Daimon S Sun Nano Lett 2009 9 1493 [28] K W Kwon M Shim J Am Chem Soc 2005 127 10269 [29] H Yu M Chen P M Rice S X Wang R L White S Sun Nano Lett 2005 5 379 [30] J S Choi Y W Jun S I Yeon H C Kim J S Shin J Cheon J Am Chem Soc 2006 128 15982 [31] S H Choi H B Na Y I Park K An S G Kwon Y Jang M Park J Moon J S Son I CSong W K Moon T Hyeon J Am Chem Soc 2008 130 15573 [32] H W Gu R K Zheng X X Zhang B Xu J Am Chem Soc 2004 126 5664
66
1- Preparation of Fe3O4SiO2Layered Double Hydroxide CoreminusShell
Microspheres for Magnetic Separation of Proteins J Am ChemSoc (2012)
18 134(2) 1071-7
Ni2+
2- Magnetic Nanocomposite Spheres Decorated with NiO Nanoparticles for a
Magnetically Recyclable Protein Separation System J Kim Y Piao N Lee Y
Park I-H Lee J-H Lee Adv Mater (2010) 22 57ndash60
His-tagged protein purification
Purification of His6-lipase using Fe3O4AundashLAndashIDAndashCu2+ NPs with various
amounts of NPs (lane 1 01 mg lane 2 02 mg lane 3
63
Synthetic procedure for the coreshell structure spheres
64
glutaraldehyde
(GLH)
Reference[1] H W Gu Z M Yang J H Gao C K Chang B Xu J Am Chem Soc 2005 127 34
[2] A Hutten D Sudfeld I Ennen G Reiss K Wojczykowski P Jutzi J Magn Magn Mater 2005
293 93
[3] G S Chaubey C Barcena N Poudyal C B Rong J M Gao S Sun J P Liu J Am Chem
Soc 2007 129 7214
[4] C W Kim Y H Kim H G Cha D K Lee Y S Kang J NanosciNanotechnol 2006 6 3417
[5] Y W Jun J S Choi J Cheon Angew Chem Int Ed 2006 45 3414
[6] X Michalet F F Pinaud L A Bentolila J M Tsay S Doose J J Li G Sundaresan A M Wu
S S Gambhir S Weiss Science 2005 307 538
[7] M Hu J Y Chen Z Y Li L Au G V Hartland X D Li M Marquez Y N Xia Chem Soc
Rev 2006 35 1084
[8] M Vallet-Regi F Balas D Arcos Angew Chem Int Ed 2007 46 7548
[9] S T Selvan P K Patra C Y Ang J Y Ying Angew Chem Int Ed 2007 46 2448
[10] I S Lee N Lee J Park B H Kim Y W Yi T Kim T K Kim I H Lee S R Paik T
Hyeon J Am Chem Soc 2006 128 10658
[11] Y S Lin S H Wu Y Hung Y H Chou C Chang M L Lin C P Tsai C Y Mou Chem
Mater 2006 18 5170
[12] B H Wu H Zhang C Chen S C Lin N F Zheng Nano Res 2009 2 975
[13] J Kim J E Lee J Lee J H Yu B C Kim K An Y Hwang C Shin J G Park J Kim T
Hyeon J Am Chem Soc 2006 128 688
[14] S Santra H S Yang P H Holloway J T Stanley R A Mericle J Am Chem Soc 2005 127
1656
[15] D K Yi S T Selvan S S Lee G C Papaefthymiou D Kundaliya J Y Ying J Am Chem
Soc 2005 127 4990 65
[16] J H Gao B Zhang Y Gao Y Pan X X Zhang B Xu J Am Chem Soc 2007 129 11928 [17] J H Gao W Zhang P B Huang B Zhang X X Zhang B Xu J Am Chem Soc 2008 130 3710 [18] J H Gao G L Liang B Zhang Y Kuang X X Zhang B Xu J Am Chem Soc 2007 129 1428 [19] J H Gao G L Liang J S Cheung Y Pan Y Kuang F Zhao B Zhang X X Zhang E X Wu B Xu J Am Chem Soc 2008 130 11828 [20] A R Kortan R Hull R L Opila M G Bawendi M L Steigerwald P J Carroll L E Brus JAm Chem Soc 1990 112 1327 [21] X G Peng M C Schlamp A V Kadavanich A P Alivisatos J Am Chem Soc 1997 119 7019 [22] S Kim B Fisher H J Eisler M Bawendi J Am Chem Soc 2003 125 11466[23] H Zeng J Li Z L Wang J P Liu S Sun Nano Lett 2004 4 187 [24] H Zeng S Sun J Li Z L Wang J P Liu Appl Phys Lett 2004 85 792 [25] Z Xu Y Hou S Sun J Am Chem Soc 2007 129 8698 [26] Y H Lin N Kim J Q Wang C J Zhong J Phys Chem B 2005 109 21593 [27] C Wang H Daimon S Sun Nano Lett 2009 9 1493 [28] K W Kwon M Shim J Am Chem Soc 2005 127 10269 [29] H Yu M Chen P M Rice S X Wang R L White S Sun Nano Lett 2005 5 379 [30] J S Choi Y W Jun S I Yeon H C Kim J S Shin J Cheon J Am Chem Soc 2006 128 15982 [31] S H Choi H B Na Y I Park K An S G Kwon Y Jang M Park J Moon J S Son I CSong W K Moon T Hyeon J Am Chem Soc 2008 130 15573 [32] H W Gu R K Zheng X X Zhang B Xu J Am Chem Soc 2004 126 5664
66
His-tagged protein purification
Purification of His6-lipase using Fe3O4AundashLAndashIDAndashCu2+ NPs with various
amounts of NPs (lane 1 01 mg lane 2 02 mg lane 3
63
Synthetic procedure for the coreshell structure spheres
64
glutaraldehyde
(GLH)
Reference[1] H W Gu Z M Yang J H Gao C K Chang B Xu J Am Chem Soc 2005 127 34
[2] A Hutten D Sudfeld I Ennen G Reiss K Wojczykowski P Jutzi J Magn Magn Mater 2005
293 93
[3] G S Chaubey C Barcena N Poudyal C B Rong J M Gao S Sun J P Liu J Am Chem
Soc 2007 129 7214
[4] C W Kim Y H Kim H G Cha D K Lee Y S Kang J NanosciNanotechnol 2006 6 3417
[5] Y W Jun J S Choi J Cheon Angew Chem Int Ed 2006 45 3414
[6] X Michalet F F Pinaud L A Bentolila J M Tsay S Doose J J Li G Sundaresan A M Wu
S S Gambhir S Weiss Science 2005 307 538
[7] M Hu J Y Chen Z Y Li L Au G V Hartland X D Li M Marquez Y N Xia Chem Soc
Rev 2006 35 1084
[8] M Vallet-Regi F Balas D Arcos Angew Chem Int Ed 2007 46 7548
[9] S T Selvan P K Patra C Y Ang J Y Ying Angew Chem Int Ed 2007 46 2448
[10] I S Lee N Lee J Park B H Kim Y W Yi T Kim T K Kim I H Lee S R Paik T
Hyeon J Am Chem Soc 2006 128 10658
[11] Y S Lin S H Wu Y Hung Y H Chou C Chang M L Lin C P Tsai C Y Mou Chem
Mater 2006 18 5170
[12] B H Wu H Zhang C Chen S C Lin N F Zheng Nano Res 2009 2 975
[13] J Kim J E Lee J Lee J H Yu B C Kim K An Y Hwang C Shin J G Park J Kim T
Hyeon J Am Chem Soc 2006 128 688
[14] S Santra H S Yang P H Holloway J T Stanley R A Mericle J Am Chem Soc 2005 127
1656
[15] D K Yi S T Selvan S S Lee G C Papaefthymiou D Kundaliya J Y Ying J Am Chem
Soc 2005 127 4990 65
[16] J H Gao B Zhang Y Gao Y Pan X X Zhang B Xu J Am Chem Soc 2007 129 11928 [17] J H Gao W Zhang P B Huang B Zhang X X Zhang B Xu J Am Chem Soc 2008 130 3710 [18] J H Gao G L Liang B Zhang Y Kuang X X Zhang B Xu J Am Chem Soc 2007 129 1428 [19] J H Gao G L Liang J S Cheung Y Pan Y Kuang F Zhao B Zhang X X Zhang E X Wu B Xu J Am Chem Soc 2008 130 11828 [20] A R Kortan R Hull R L Opila M G Bawendi M L Steigerwald P J Carroll L E Brus JAm Chem Soc 1990 112 1327 [21] X G Peng M C Schlamp A V Kadavanich A P Alivisatos J Am Chem Soc 1997 119 7019 [22] S Kim B Fisher H J Eisler M Bawendi J Am Chem Soc 2003 125 11466[23] H Zeng J Li Z L Wang J P Liu S Sun Nano Lett 2004 4 187 [24] H Zeng S Sun J Li Z L Wang J P Liu Appl Phys Lett 2004 85 792 [25] Z Xu Y Hou S Sun J Am Chem Soc 2007 129 8698 [26] Y H Lin N Kim J Q Wang C J Zhong J Phys Chem B 2005 109 21593 [27] C Wang H Daimon S Sun Nano Lett 2009 9 1493 [28] K W Kwon M Shim J Am Chem Soc 2005 127 10269 [29] H Yu M Chen P M Rice S X Wang R L White S Sun Nano Lett 2005 5 379 [30] J S Choi Y W Jun S I Yeon H C Kim J S Shin J Cheon J Am Chem Soc 2006 128 15982 [31] S H Choi H B Na Y I Park K An S G Kwon Y Jang M Park J Moon J S Son I CSong W K Moon T Hyeon J Am Chem Soc 2008 130 15573 [32] H W Gu R K Zheng X X Zhang B Xu J Am Chem Soc 2004 126 5664
66
Synthetic procedure for the coreshell structure spheres
64
glutaraldehyde
(GLH)
Reference[1] H W Gu Z M Yang J H Gao C K Chang B Xu J Am Chem Soc 2005 127 34
[2] A Hutten D Sudfeld I Ennen G Reiss K Wojczykowski P Jutzi J Magn Magn Mater 2005
293 93
[3] G S Chaubey C Barcena N Poudyal C B Rong J M Gao S Sun J P Liu J Am Chem
Soc 2007 129 7214
[4] C W Kim Y H Kim H G Cha D K Lee Y S Kang J NanosciNanotechnol 2006 6 3417
[5] Y W Jun J S Choi J Cheon Angew Chem Int Ed 2006 45 3414
[6] X Michalet F F Pinaud L A Bentolila J M Tsay S Doose J J Li G Sundaresan A M Wu
S S Gambhir S Weiss Science 2005 307 538
[7] M Hu J Y Chen Z Y Li L Au G V Hartland X D Li M Marquez Y N Xia Chem Soc
Rev 2006 35 1084
[8] M Vallet-Regi F Balas D Arcos Angew Chem Int Ed 2007 46 7548
[9] S T Selvan P K Patra C Y Ang J Y Ying Angew Chem Int Ed 2007 46 2448
[10] I S Lee N Lee J Park B H Kim Y W Yi T Kim T K Kim I H Lee S R Paik T
Hyeon J Am Chem Soc 2006 128 10658
[11] Y S Lin S H Wu Y Hung Y H Chou C Chang M L Lin C P Tsai C Y Mou Chem
Mater 2006 18 5170
[12] B H Wu H Zhang C Chen S C Lin N F Zheng Nano Res 2009 2 975
[13] J Kim J E Lee J Lee J H Yu B C Kim K An Y Hwang C Shin J G Park J Kim T
Hyeon J Am Chem Soc 2006 128 688
[14] S Santra H S Yang P H Holloway J T Stanley R A Mericle J Am Chem Soc 2005 127
1656
[15] D K Yi S T Selvan S S Lee G C Papaefthymiou D Kundaliya J Y Ying J Am Chem
Soc 2005 127 4990 65
[16] J H Gao B Zhang Y Gao Y Pan X X Zhang B Xu J Am Chem Soc 2007 129 11928 [17] J H Gao W Zhang P B Huang B Zhang X X Zhang B Xu J Am Chem Soc 2008 130 3710 [18] J H Gao G L Liang B Zhang Y Kuang X X Zhang B Xu J Am Chem Soc 2007 129 1428 [19] J H Gao G L Liang J S Cheung Y Pan Y Kuang F Zhao B Zhang X X Zhang E X Wu B Xu J Am Chem Soc 2008 130 11828 [20] A R Kortan R Hull R L Opila M G Bawendi M L Steigerwald P J Carroll L E Brus JAm Chem Soc 1990 112 1327 [21] X G Peng M C Schlamp A V Kadavanich A P Alivisatos J Am Chem Soc 1997 119 7019 [22] S Kim B Fisher H J Eisler M Bawendi J Am Chem Soc 2003 125 11466[23] H Zeng J Li Z L Wang J P Liu S Sun Nano Lett 2004 4 187 [24] H Zeng S Sun J Li Z L Wang J P Liu Appl Phys Lett 2004 85 792 [25] Z Xu Y Hou S Sun J Am Chem Soc 2007 129 8698 [26] Y H Lin N Kim J Q Wang C J Zhong J Phys Chem B 2005 109 21593 [27] C Wang H Daimon S Sun Nano Lett 2009 9 1493 [28] K W Kwon M Shim J Am Chem Soc 2005 127 10269 [29] H Yu M Chen P M Rice S X Wang R L White S Sun Nano Lett 2005 5 379 [30] J S Choi Y W Jun S I Yeon H C Kim J S Shin J Cheon J Am Chem Soc 2006 128 15982 [31] S H Choi H B Na Y I Park K An S G Kwon Y Jang M Park J Moon J S Son I CSong W K Moon T Hyeon J Am Chem Soc 2008 130 15573 [32] H W Gu R K Zheng X X Zhang B Xu J Am Chem Soc 2004 126 5664
66
Reference[1] H W Gu Z M Yang J H Gao C K Chang B Xu J Am Chem Soc 2005 127 34
[2] A Hutten D Sudfeld I Ennen G Reiss K Wojczykowski P Jutzi J Magn Magn Mater 2005
293 93
[3] G S Chaubey C Barcena N Poudyal C B Rong J M Gao S Sun J P Liu J Am Chem
Soc 2007 129 7214
[4] C W Kim Y H Kim H G Cha D K Lee Y S Kang J NanosciNanotechnol 2006 6 3417
[5] Y W Jun J S Choi J Cheon Angew Chem Int Ed 2006 45 3414
[6] X Michalet F F Pinaud L A Bentolila J M Tsay S Doose J J Li G Sundaresan A M Wu
S S Gambhir S Weiss Science 2005 307 538
[7] M Hu J Y Chen Z Y Li L Au G V Hartland X D Li M Marquez Y N Xia Chem Soc
Rev 2006 35 1084
[8] M Vallet-Regi F Balas D Arcos Angew Chem Int Ed 2007 46 7548
[9] S T Selvan P K Patra C Y Ang J Y Ying Angew Chem Int Ed 2007 46 2448
[10] I S Lee N Lee J Park B H Kim Y W Yi T Kim T K Kim I H Lee S R Paik T
Hyeon J Am Chem Soc 2006 128 10658
[11] Y S Lin S H Wu Y Hung Y H Chou C Chang M L Lin C P Tsai C Y Mou Chem
Mater 2006 18 5170
[12] B H Wu H Zhang C Chen S C Lin N F Zheng Nano Res 2009 2 975
[13] J Kim J E Lee J Lee J H Yu B C Kim K An Y Hwang C Shin J G Park J Kim T
Hyeon J Am Chem Soc 2006 128 688
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